BLASTP 2.2.6 [Apr-09-2003]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

RID: 1056541962-015436-31287

Query= SPBC146.01 ||SPBP35G2.15|RNA polymerase II holoenzyme
component (predicted)|Schizosaccharomyces pombe||chr 2||Manual
         (1063 letters)


Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF 
           1,459,100 sequences; 468,613,799 total letters

 
If you have any problems or questions with the results of this search 
please refer to the BLAST FAQs

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|19112182|ref|NP_595390.1|  putative transcriptional regul...   825   0.0  
gi|7522544|pir||T39415  probable transcription regulator SPB...   748   0.0  
gi|7493813|pir||T18235  transcription activator GAL11 homolo...    54   2e-05
gi|1169834|sp|P32257|GALY_KLULA  TRANSCRIPTION REGULATORY PR...    50   3e-04
gi|171550|gb|AAA34622.1|  auxiliary activator                      45   0.008
gi|6324522|ref|NP_014591.1|  Regulates transcription of a di...    45   0.009
gi|984193|emb|CAA62537.1|  transcriptional activator [Saccha...    44   0.016

Alignments

>gi|19112182|ref|NP_595390.1| putative transcriptional regulatory protein [Schizosaccharomyces
           pombe]
 gi|7573208|emb|CAB87377.1| SPBP35G2.15 [Schizosaccharomyces pombe]
          Length = 653

 Score =  825 bits (2132), Expect = 0.0
 Identities = 491/653 (75%), Positives = 491/653 (75%)

Query: 1   MRDRALPCKCMPSFFKTLHLTFVFSAQTLRIISPSISEVQLMNMALSFERQAFDGASSKN 60
           MRDRALPCKCMPSFFKTLHLTFVFSAQTLRIISPSISEVQLMNMALSFERQAFDGASSKN
Sbjct: 1   MRDRALPCKCMPSFFKTLHLTFVFSAQTLRIISPSISEVQLMNMALSFERQAFDGASSKN 60

Query: 61  EYLTTCGKKTAQLRDQIRDTLQATQMKQMPSVYNNAGNVGALPTAGPNRLANNPRVMPRL 120
           EYLTTCGKKTAQLRDQIRDTLQATQMKQMPSVYNNAGNVGALPTAGPNRLANNPRVMPRL
Sbjct: 61  EYLTTCGKKTAQLRDQIRDTLQATQMKQMPSVYNNAGNVGALPTAGPNRLANNPRVMPRL 120

Query: 121 QNQNVPMQAGMQQFARNMKLTPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXASSPT 180
           QNQNVPMQAGMQQFARNMKLTP                                 ASSPT
Sbjct: 121 QNQNVPMQAGMQQFARNMKLTPQQRQFLLQQSQIQQQRQQQQQQSQQPQQTQQPQASSPT 180

Query: 181 APNTEANQQRSGSVPGRIVPAXXXXXXXXXXXXITALLARNGNPPIPMQKLQSMPPARLI 240
           APNTEANQQRSGSVPGRIVPA            ITALLARNGNPPIPMQKLQSMPPARLI
Sbjct: 181 APNTEANQQRSGSVPGRIVPALTQQQLNNLCNQITALLARNGNPPIPMQKLQSMPPARLI 240

Query: 241 SIYQNQIQKFRSLQHMXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKQAPQNAFFPN 300
           SIYQNQIQKFRSLQHM                                 KQAPQNAFFPN
Sbjct: 241 SIYQNQIQKFRSLQHMQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQKQAPQNAFFPN 300

Query: 301 PQGNVGAQSLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEMLNIPVPKQLF 360
           PQGNVGAQSL                                      EMLNIPVPKQLF
Sbjct: 301 PQGNVGAQSLQSMSPQDQPSTQQQQPQRTAAPPNNPNVNATNNNRINIEMLNIPVPKQLF 360

Query: 361 DQIPNLPPNVKIWRDVLELGQSQRLPPEQLKLIGMLYRKXXXXXXXXXXXXXNKIQNARM 420
           DQIPNLPPNVKIWRDVLELGQSQRLPPEQLKLIGMLYRK             NKIQNARM
Sbjct: 361 DQIPNLPPNVKIWRDVLELGQSQRLPPEQLKLIGMLYRKHLQIILQHRQQQLNKIQNARM 420

Query: 421 NSQNAPNTNKLGNPQPDNTGNPXXXXXXXXXXXXXXXXXXLHRTSNPTSASVTSQNGQQP 480
           NSQNAPNTNKLGNPQPDNTGNP                  LHRTSNPTSASVTSQNGQQP
Sbjct: 421 NSQNAPNTNKLGNPQPDNTGNPQAFSQQAFAQQQQQQQQQLHRTSNPTSASVTSQNGQQP 480

Query: 481 INTKLSANAAKTNYQSYLTNKARNATQPTQPPVSQVDYSNNLPPNLDXXXXXXXXXXXXX 540
           INTKLSANAAKTNYQSYLTNKARNATQPTQPPVSQVDYSNNLPPNLD             
Sbjct: 481 INTKLSANAAKTNYQSYLTNKARNATQPTQPPVSQVDYSNNLPPNLDTSSTFRSSASPPS 540

Query: 541 XXTKAGNEALSVPLSGARNTAASRPTNLAAGNSSASIVQQLLECGGTMGQQKMTSRIREL 600
             TKAGNEALSVPLSGARNTAASRPTNLAAGNSSASIVQQLLECGGTMGQQKMTSRIREL
Sbjct: 541 AFTKAGNEALSVPLSGARNTAASRPTNLAAGNSSASIVQQLLECGGTMGQQKMTSRIREL 600

Query: 601 TERVMHSLMRPVPLDLPHDQKVMIASLIKSAYPMFSRTNQLICLFYCLTGNEE 653
           TERVMHSLMRPVPLDLPHDQKVMIASLIKSAYPMFSRTNQLICLFYCLTGNEE
Sbjct: 601 TERVMHSLMRPVPLDLPHDQKVMIASLIKSAYPMFSRTNQLICLFYCLTGNEE 653



>gi|7522544|pir||T39415 probable transcription regulator SPBC146.01 - fission yeast
            (Schizosaccharomyces pombe) (fragment)
 gi|5441483|emb|CAB46754.1| SPBC146.01 [Schizosaccharomyces pombe]
          Length = 445

 Score =  748 bits (1930), Expect = 0.0
 Identities = 419/445 (94%), Positives = 419/445 (94%)

Query: 619  DQKVMIASLIKSAYPMFSRTNQLICLFYCLTGNEEATIQLIQMRHIFKLQLEGLQQGVFT 678
            DQKVMIASLIKSAYPMFSRTNQLICLFYCLTGNEEATIQLIQMRHIFKLQLEGLQQGVFT
Sbjct: 1    DQKVMIASLIKSAYPMFSRTNQLICLFYCLTGNEEATIQLIQMRHIFKLQLEGLQQGVFT 60

Query: 679  CAPQTLAKIKEKTSRYFAFVKAQLLRLHHEVNNNNMSIQNALAHISSLRTASINXXXXXX 738
            CAPQTLAKIKEKTSRYFAFVKAQLLRLHHEVNNNNMSIQNALAHISSLRTASIN      
Sbjct: 61   CAPQTLAKIKEKTSRYFAFVKAQLLRLHHEVNNNNMSIQNALAHISSLRTASINQQQQPQ 120

Query: 739  XXXXXXXXXXXXXXXXXXXXNVRPINGSIPNAQPSVPGQAQPAAKANSVGNTSFPVDSKL 798
                                NVRPINGSIPNAQPSVPGQAQPAAKANSVGNTSFPVDSKL
Sbjct: 121  PQSQQQQQASQFPQAPSVSSNVRPINGSIPNAQPSVPGQAQPAAKANSVGNTSFPVDSKL 180

Query: 799  AFQSLDVSQPDLQAKQKIASQVMKHGLKPEDLKLPPSKKKKIENLSTVQKPKDSVVNTPD 858
            AFQSLDVSQPDLQAKQKIASQVMKHGLKPEDLKLPPSKKKKIENLSTVQKPKDSVVNTPD
Sbjct: 181  AFQSLDVSQPDLQAKQKIASQVMKHGLKPEDLKLPPSKKKKIENLSTVQKPKDSVVNTPD 240

Query: 859  VIMSSVDEIPSSVSPGTIAKEEGMAKAREEAIANPLKYAIDAFVAVDHEEEVSAIKSSQT 918
            VIMSSVDEIPSSVSPGTIAKEEGMAKAREEAIANPLKYAIDAFVAVDHEEEVSAIKSSQT
Sbjct: 241  VIMSSVDEIPSSVSPGTIAKEEGMAKAREEAIANPLKYAIDAFVAVDHEEEVSAIKSSQT 300

Query: 919  PSSILKTPQSFFIPPSTPDLSFTDNKNSLSPSNILSLDGKFSFNDDSELWADLGNEINSE 978
            PSSILKTPQSFFIPPSTPDLSFTDNKNSLSPSNILSLDGKFSFNDDSELWADLGNEINSE
Sbjct: 301  PSSILKTPQSFFIPPSTPDLSFTDNKNSLSPSNILSLDGKFSFNDDSELWADLGNEINSE 360

Query: 979  IGFLKEPDTMNLALDADKDKTKMQNKLTQINFDESCFLDPAIDDKDPWNEMLHEKKVLLK 1038
            IGFLKEPDTMNLALDADKDKTKMQNKLTQINFDESCFLDPAIDDKDPWNEMLHEKKVLLK
Sbjct: 361  IGFLKEPDTMNLALDADKDKTKMQNKLTQINFDESCFLDPAIDDKDPWNEMLHEKKVLLK 420

Query: 1039 QLQVGNEDEDNIAFPTSTNIWQVVI 1063
            QLQVGNEDEDNIAFPTSTNIWQVVI
Sbjct: 421  QLQVGNEDEDNIAFPTSTNIWQVVI 445



>gi|7493813|pir||T18235 transcription activator GAL11 homolog - yeast (Candida albicans)
 gi|3859719|emb|CAA21993.1| possible regulatory protein [Candida albicans]
          Length = 1145

 Score = 53.9 bits (128), Expect = 2e-05
 Identities = 39/146 (26%), Positives = 72/146 (49%), Gaps = 10/146 (6%)

Query: 559 NTAASRPTNLAAGNSSASIVQQLLECGGTMGQQKMTSRIRELTERVMHS--LMRPVPLDL 616
           N    RP    A  + +SI+  L +        ++  R+R+L E V  +  ++R V   L
Sbjct: 579 NINRGRPVTAPANITMSSILPPLTD--------EVKLRLRQLIEEVSRNNVVLRDVTSLL 630

Query: 617 PHDQKVMIASLIKSAYPMFSRTNQLICLFYCLTGNEEATIQLIQMRHIFKLQLEGLQQGV 676
            +  K  +   +      +   + +I  FY LT N + T +LIQM+++ K  L+ L +G+
Sbjct: 631 SNQDKTTVRDSMNRIQEQYGNVDSIISYFYLLTKNGDGTKRLIQMKYMTKNILDNLHRGI 690

Query: 677 FTCAPQTLAKIKEKTSRYFAFVKAQL 702
           +   P  L K++ + ++YF +VK Q+
Sbjct: 691 YLAGPDLLEKVRSQYAKYFEYVKEQI 716



>gi|1169834|sp|P32257|GALY_KLULA TRANSCRIPTION REGULATORY PROTEIN GAL11
 gi|101140|pir||S19033 transcription activator GAL11 - yeast (Kluyveromyces marxianus var.
           lactis) (fragment)
 gi|295684|gb|AAA35254.1| GAL11 transcription activator
          Length = 1008

 Score = 49.7 bits (117), Expect = 3e-04
 Identities = 31/94 (32%), Positives = 49/94 (52%)

Query: 616 LPHDQKVMIASLIKSAYPMFSRTNQLICLFYCLTGNEEATIQLIQMRHIFKLQLEGLQQG 675
           L +DQK  I S + +   +FS     I   Y LT NEE   QL+Q+R + +  +E   +G
Sbjct: 501 LTNDQKQQIRSKLHANQQLFSSVESFIPTLYMLTRNEEHIRQLLQIRMLTREIMEKAVRG 560

Query: 676 VFTCAPQTLAKIKEKTSRYFAFVKAQLLRLHHEV 709
           VF   P  + K+  +  +Y+ + K QLLR   ++
Sbjct: 561 VFLVEPNVVDKVIFRYQKYYEYTKEQLLRRQQQL 594



>gi|171550|gb|AAA34622.1| auxiliary activator
          Length = 1081

 Score = 44.7 bits (104), Expect = 0.008
 Identities = 19/50 (38%), Positives = 31/50 (62%)

Query: 349 EMLNIPVPKQLFDQIPNLPPNVKIWRDVLELGQSQRLPPEQLKLIGMLYR 398
           +M   P+PKQL  +IPN+PPN+  W+ V  L Q + L P+ ++    +Y+
Sbjct: 172 QMKVAPIPKQLLQRIPNIPPNINTWQQVTALAQQKLLTPQDMEAAKEVYK 221



>gi|6324522|ref|NP_014591.1| Regulates transcription of a diverse array of genes. Required for
           mating and sporulation.; Gal11p [Saccharomyces
           cerevisiae]
 gi|2507028|sp|P19659|GALY_YEAST TRANSCRIPTION REGULATORY PROTEIN GAL11
 gi|2133177|pir||S66736 transcription activator GAL11 - yeast (Saccharomyces cerevisiae)
 gi|1419856|emb|CAA99056.1| ORF YOL051w [Saccharomyces cerevisiae]
          Length = 1081

 Score = 44.7 bits (104), Expect = 0.009
 Identities = 19/50 (38%), Positives = 31/50 (62%)

Query: 349 EMLNIPVPKQLFDQIPNLPPNVKIWRDVLELGQSQRLPPEQLKLIGMLYR 398
           +M   P+PKQL  +IPN+PPN+  W+ V  L Q + L P+ ++    +Y+
Sbjct: 172 QMKVAPIPKQLLQRIPNIPPNINTWQQVTALAQQKLLTPQDMEAAKEVYK 221



>gi|984193|emb|CAA62537.1| transcriptional activator [Saccharomyces cerevisiae]
          Length = 351

 Score = 43.9 bits (102), Expect = 0.016
 Identities = 19/50 (38%), Positives = 31/50 (62%)

Query: 349 EMLNIPVPKQLFDQIPNLPPNVKIWRDVLELGQSQRLPPEQLKLIGMLYR 398
           +M   P+PKQL  +IPN+PPN+  W+ V  L Q + L P+ ++    +Y+
Sbjct: 172 QMKVAPIPKQLLQRIPNIPPNINTWQQVTALAQQKLLTPQDMEAAKEVYK 221





  Database: All non-redundant GenBank CDS
  translations+PDB+SwissProt+PIR+PRF
    Posted date:  Jun 25, 2003  2:03 AM
  Number of letters in database: 468,613,799
  Number of sequences in database:  1,459,100
  
Lambda     K      H
   0.313    0.129    0.362 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 780,418,599
Number of Sequences: 1459100
Number of extensions: 27772833
Number of successful extensions: 65277
Number of sequences better than  1.0: 23
Number of HSP's better than  1.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 16
Number of HSP's that attempted gapping in prelim test: 65232
Number of HSP's gapped (non-prelim): 64
length of query: 1063
length of database: 468,613,799
effective HSP length: 134
effective length of query: 929
effective length of database: 273,094,399
effective search space: 253704696671
effective search space used: 253704696671
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 87 (38.1 bits)