Legend:
Sequences with E-value BETTER than threshold 401 e-111
400 e-110
399 e-110
395 e-109
393 e-108
378 e-104
195 5e-49
59 8e-08
52 1e-05
48 2e-04
45 0.001
Sequences with E-value WORSE than threshold ref|NP_014324.1| Imp4p [Saccharomyces cerevisiae] >gi|1730744|sp... 44 0.003
emb|CAC11120.1| (AL357374) bA353C18.4 (novel protein) [Homo sapi... 41 0.022
emb|CAB77726.1| (AL161492) hypothetical protein [Arabidopsis tha... 41 0.028
dbj|BAB14983.1| (AK024740) unnamed protein product [Homo sapiens] 40 0.052
gb|AAD14602.1| (AF092910) stage specific peptide 24 [Trypanosoma... 39 0.060
gb|AAG52427.1|AC011622_15 (AC011622) putative U3 small nucleolar... 39 0.063
pir||T39008 probable mating protein - fission yeast (Schizosacch... 39 0.087
pir||T50616 hypothetical protein DKFZp761G0415.1 - human (fragment) 39 0.093
pir||T01938 hypothetical protein F11O4.6 - Arabidopsis thaliana ... 38 0.16
gb|AAF53162.1| (AE003635) CG6712 gene product [Drosophila melano... 37 0.22
dbj|BAB14086.1| (AK022537) unnamed protein product [Homo sapiens] 37 0.39
gb|AAD02810.1| (AF062396) protein phosphatase 2A regulatory subu... 37 0.40
gb|AAD16459.1| (AF102805) Peter Pan [Drosophila melanogaster] 36 0.66
gb|AAF55851.1| (AE003733) ppan gene product [Drosophila melanoga... 36 0.75
sp|O14180|YDS4_SCHPO HYPOTHETICAL 35.8 KD PROTEIN C4F8.04 IN CHR... 34 2.6
gb|AAG52369.1|AC011765_21 (AC011765) putative cytochrome P450; 6... 34 2.6
sp|P07788|COTA_BACSU SPORE COAT PROTEIN A >gi|7475834|pir||F6960... 34 4.0
dbj|BAA22774.1| (AB007638) spore coat proein A [Bacillus subtilis] 34 4.0
pir||T32923 hypothetical protein K09H9.6 - Caenorhabditis elegan... 33 5.4
Alignments
>emb|CAC01521.1| (AL391034) conserved hypothetical protein [Schizosaccharomyces
pombe]
Length = 295
Score = 401 bits (1021), Expect = e-111
Identities = 124/287 (43%), Positives = 177/287 (61%), Gaps = 16/287 (5%)
Query: 28 SEQEATAGEEFIVPPPVSEGKWKN--------KERVLIFSSRGINFRTRHLMQDLRSLMP 79
SE+ +E PV G+ K++VL+ SSRG+ +R RHL+ DL S+MP
Sbjct: 10 SERAPKNEDEDEEYVPVQNGQGNKHSAGFVPIKQKVLVLSSRGVTYRQRHLLNDLVSMMP 69
Query: 80 HSRAETKMDRKDKLFVVNEVCEMKNCNKCIYFEAKKKQDLYMWLSNSPEGPSAKFLVQNI 139
HS+ ++K+D KD+L+ +NE+ E+ NCN +FE+++++DLY+ ++ +P GP+ KF V+N+
Sbjct: 70 HSKKDSKLDSKDRLYQLNELAELYNCNNIFFFESRRREDLYLHIARAPNGPTVKFHVENL 129
Query: 140 HTLAELKMSGNCLKGSRPILSFDPAFDREPQYALLKELLTQIFGTPRYHPRSQPFVDHIF 199
HT+ EL M+GN LKGSRPILSFD FD P ++KELL Q FG P+ RS+PF+D +
Sbjct: 130 HTMDELNMTGNALKGSRPILSFDKTFDTAPHLKVVKELLQQTFGIPKGARRSKPFIDRVC 189
Query: 200 TFSIADNRIWFRNYQIIEED------AALVEIGPRFVLNLIKIFKGSFGGPTLYENPHYQ 253
T +IAD +IWFRNY+I E + L+EIGPRFV+ +I I +GSFGGP +Y+N +
Sbjct: 190 TLTIADGKIWFRNYEIRENEDKSKDPVTLIEIGPRFVMTIINILEGSFGGPVIYKNDTFV 249
Query: 254 SPNMHRRMIRLATAAXXXXXXXXXXXXXXXXXXDRPVIPVDPTEAVF 300
S M R IR A + VIP DP + VF
Sbjct: 250 SSTMVRAAIR--NQAAQRYVNRQESKLERQVRAQQNVIPEDPLDNVF 294
>gb|AAF47877.2| (AE003480) CG11583 gene product [Drosophila melanogaster]
Length = 359
Score = 400 bits (1017), Expect = e-110
Identities = 135/230 (58%), Positives = 179/230 (77%), Gaps = 2/230 (0%)
Query: 39 IVPPPVSEGKWKNKERVLIFSSRGINFRTRHLMQDLRSLMPHSRAETKMDRKDKLFVVNE 98
+VP + KW NK+RVL+FS+RGI+ R RHLM+D+++LMPH R E+KM+R L VVNE
Sbjct: 36 VVPKKARKEKWVNKQRVLVFSARGISHRDRHLMKDIKTLMPHHRPESKMERSKTLSVVNE 95
Query: 99 VCEMKNCNKCIYFEAKKKQDLYMWLSNS--PEGPSAKFLVQNIHTLAELKMSGNCLKGSR 156
+CEMK+CNK + FE ++K+DLYMW+SN+ GPSAKFL++NIHT+AELKM+GNCL+GSR
Sbjct: 96 MCEMKHCNKAMLFEGRRKRDLYMWISNTSGSTGPSAKFLIENIHTMAELKMTGNCLRGSR 155
Query: 157 PILSFDPAFDREPQYALLKELLTQIFGTPRYHPRSQPFVDHIFTFSIADNRIWFRNYQII 216
P+LSFD FD P LLKEL Q + P +HP+SQPFVDH+FTF+ D RIWFRN+QI+
Sbjct: 156 PLLSFDSKFDELPHLKLLKELFVQTYSVPNHHPKSQPFVDHVFTFTYLDKRIWFRNFQIL 215
Query: 217 EEDAALVEIGPRFVLNLIKIFKGSFGGPTLYENPHYQSPNMHRRMIRLAT 266
ED L E+GPR+V+N +KIF GSF G T++ENP Y SP+ R++++ A
Sbjct: 216 SEDGGLSEVGPRYVMNPVKIFDGSFTGKTIWENPDYVSPSKQRQVLKKAA 265
>gb|AAF35412.1| (AC024081) unknown protein [Arabidopsis thaliana]
Length = 315
Score = 399 bits (1015), Expect = e-110
Identities = 122/281 (43%), Positives = 170/281 (60%), Gaps = 14/281 (4%)
Query: 1 MSAYKRKRGSLPEVATNTKKAKKQLAGSEQEATAGEEFIVPPPVSEGKWKNKERVLIFSS 60
M ++ ++ ++ E+ P ++NKE+VL+ S
Sbjct: 1 MGRKRKHSETVTAAPVKDSAPERPQRTLLGWKDKKEDAENSKPAYASVFRNKEKVLVTCS 60
Query: 61 RGINFRTRHLMQDLRSLMPHSRAETKMDRK-DKLFVVNEVCEMKNCNKCIYFEAKKKQDL 119
R INFR RHLM ++ SL+PH + ++K++ K + +NE+ E+K + C++FE +K +DL
Sbjct: 61 RRINFRYRHLMLNMVSLLPHCKKDSKVEAKSSRGATLNELIELKGSSSCLFFECRKHKDL 120
Query: 120 YMWLSNSPEGPSAKFLVQNIHTLAELKMSGNCLKGSRPILSFDPAFDREPQYALLKELLT 179
YMW+ SP GPS KFLV +HT+ ELK++GN LKGSRP+L+F F+ + + LLKE+LT
Sbjct: 121 YMWMVKSPGGPSVKFLVNAVHTMEELKLTGNHLKGSRPLLTFSSNFENDAHWKLLKEMLT 180
Query: 180 QIFGTPRYHPRSQPFVDHIFTFSIADNRIWFRNYQII----EEDA---------ALVEIG 226
QIFG P H +S+P+ DH+F FSI D+ IWFRNYQI E D L+E+G
Sbjct: 181 QIFGIPEGHRKSKPYHDHVFVFSIVDDHIWFRNYQISVPHNESDKIARGDLDKMTLIEVG 240
Query: 227 PRFVLNLIKIFKGSFGGPTLYENPHYQSPNMHRRMIRLATA 267
PRF LN IKIF GSFGG TLYENP Y SPN R + + A
Sbjct: 241 PRFCLNPIKIFGGSFGGTTLYENPFYVSPNQIRALEKRNKA 281
>ref|NP_014565.1| Essential nucleolar protein required for biogenesis of the 60S
ribosomal subunit; homologue of BRIX (Biogenesis of
ribosomes in Xenopus); Brx1p [Saccharomyces cerevisiae]
pir||S66770 probable membrane protein YOL077c - yeast (Saccharomyces
cerevisiae)
emb|CAA99087.1| (Z74819) ORF YOL077c [Saccharomyces cerevisiae]
Length = 291
Score = 395 bits (1005), Expect = e-109
Identities = 124/263 (47%), Positives = 164/263 (62%), Gaps = 14/263 (5%)
Query: 48 KWKNKERVLIFSSRGINFRTRHLMQDLRSLMPHSRAETKMDRKDKLFVVNEVCEMKNCNK 107
++ NK+R L+ SSRG+N+R RHL+QDL L+PHSR E K+D K L +NE+ E+ NCN
Sbjct: 26 QFMNKQRTLLISSRGVNYRHRHLIQDLSGLLPHSRKEPKLDTKKDLQQLNEIAELYNCNN 85
Query: 108 CIYFEAKKKQDLYMWLSNSPEGPSAKFLVQNIHTLAELKMSGNCLKGSRPILSFDPAFDR 167
++FEA+K QDLY+WLS P GP+ KF +QN+HT+ EL +GNCLKGSRP+LSFD F+
Sbjct: 86 VLFFEARKHQDLYLWLSKPPNGPTIKFYIQNLHTMDELNFTGNCLKGSRPVLSFDQRFES 145
Query: 168 EPQYALLKELLTQIFGTPRYHPRSQPFVDHIFTFSIADNRIWFRNYQII----------- 216
P Y L+KELL F P +S+PF+DH+ +FSI D++IW R Y+I
Sbjct: 146 SPHYQLIKELLVHNFCVPPNARKSKPFIDHVMSFSIVDDKIWVRTYEISHSTKNKEEYED 205
Query: 217 -EEDAALVEIGPRFVLNLIKIFKGSFGGPTLYENPHYQSPNMHRRMIRLATAAXXXXXXX 275
EED +LVEIGPRFV+ +I I +GSFGGP +YEN Y SPN+ R I+ A
Sbjct: 206 GEEDISLVEIGPRFVMTVILILEGSFGGPKIYENKQYVSPNVVRAQIKQQAA--EEAKSR 263
Query: 276 XXXXXXXXXXXDRPVIPVDPTEA 298
V+ DP
Sbjct: 264 AEAAVERKIKRRENVLAADPLSN 286
>gb|AAG52272.1|AC019018_9 (AC019018) unknown protein; 80333-82175 [Arabidopsis thaliana]
Length = 320
Score = 393 bits (999), Expect = e-108
Identities = 124/262 (47%), Positives = 173/262 (65%), Gaps = 16/262 (6%)
Query: 20 KAKKQLAGSEQEATAGEEFIVPPPVSEGKWKNKERVLIFSSRGINFRTRHLMQDLRSLMP 79
+ K+ L G + ++ E ++ +KNKE+VL+ SR I+FR R LM ++ SL+P
Sbjct: 26 RPKRTLLGWKDKSEGEAEKA--KALTSSGFKNKEKVLVTCSRRISFRYRSLMLNIVSLLP 83
Query: 80 HSRAETKMDRK-DKLFVVNEVCEMKNCNKCIYFEAKKKQDLYMWLSNSPEGPSAKFLVQN 138
H + ++K++ K K +NE+ E+KN N C++FE +K +DLYMW+ SP GPS KFLV+
Sbjct: 84 HCKKDSKVEAKSSKGATLNELIELKNSNSCLFFECRKHKDLYMWMVKSPNGPSVKFLVKA 143
Query: 139 IHTLAELKMSGNCLKGSRPILSFDPAFDREPQYALLKELLTQIFGTPRYHPRSQPFVDHI 198
+H + E+K++GN LKGSRP+L+F FD++ + LLKE+LTQ+FG P+ H +S+P+ DH+
Sbjct: 144 VHAMEEMKLTGNHLKGSRPLLTFSSNFDKDAHWKLLKEMLTQVFGIPKEHRKSKPYHDHV 203
Query: 199 FTFSIADNRIWFRNYQII----EEDA---------ALVEIGPRFVLNLIKIFKGSFGGPT 245
F FSI D IWFRNYQI E D L+E+GPRF LN IKIF GSFGGPT
Sbjct: 204 FVFSIVDEHIWFRNYQISVPHNESDKIAKGGLDKMTLIEVGPRFCLNPIKIFAGSFGGPT 263
Query: 246 LYENPHYQSPNMHRRMIRLATA 267
LYENP Y SPN R + + A
Sbjct: 264 LYENPLYVSPNQIRALEKRNKA 285
>sp|P34524|YM63_CAEEL HYPOTHETICAL 40.2 KD PROTEIN K12H4.3 IN CHROMOSOME III
pir||S44853 K12H4.3 protein - Caenorhabditis elegans
gb|AAA28097.1| (L14331) coded for by C. elegans cDNAs GenBank: CE5D1 (Z14791),
CEL01F1 (M88817), CEL04B5(M88849), and CEL04C1(M75812);
putative [Caenorhabditis elegans]
Length = 352
Score = 378 bits (961), Expect = e-104
Identities = 125/264 (47%), Positives = 184/264 (69%), Gaps = 4/264 (1%)
Query: 2 SAYKRKRGSLPEVATNTKKAKKQLAGSEQEATAGEEFIVPPPVSEGKWKNKERVLIFSSR 61
SA+++ ++ + ++ + E W N+ERVL+ SR
Sbjct: 15 SAHQKMEWEAAGAKDSSSDDSSDESDNDDQPKQATEET---RKRAELWTNRERVLVLCSR 71
Query: 62 GINFRTRHLMQDLRSLMPHSRAETKMDRKDKLFVVNEVCEMKNCNKCIYFEAKKKQDLYM 121
G + RTR+LM+D++ L+PH++ ++K+D++ L V+NE+ EMKNC K +YFE++K++D Y+
Sbjct: 72 GADVRTRYLMKDIKDLLPHAKGDSKLDQQKSLNVLNEIAEMKNCTKVMYFESRKRKDTYL 131
Query: 122 WLSNSPEGPSAKFLVQNIHTLAELKMSGNCLKGSRPILSFDPAFDREPQYALLKELLTQI 181
W+SN +GPS KFLV N+HT+ ELKMSGNCL+ SRP+LSFD AFD++PQ L+K +L Q
Sbjct: 132 WMSNVEKGPSIKFLVHNVHTMKELKMSGNCLRASRPVLSFDDAFDKKPQLKLIKAVLMQT 191
Query: 182 FGTPRYHPRSQPFVDHIFTFSIAD-NRIWFRNYQIIEEDAALVEIGPRFVLNLIKIFKGS 240
GTP +HPRSQPFVDH+F FS+ + ++IWFRN+QI++E L E+GPRFVL ++++F GS
Sbjct: 192 LGTPHHHPRSQPFVDHVFNFSVGEGDKIWFRNFQIVDESLQLQEVGPRFVLEMVRLFAGS 251
Query: 241 FGGPTLYENPHYQSPNMHRRMIRL 264
F G LY+NP+Y SPN+ RR R
Sbjct: 252 FEGAVLYDNPNYVSPNVIRREHRK 275
>ref|NP_060791.1| hypothetical protein FLJ11100 [Homo sapiens]
ref|XP_003882.1| hypothetical protein FLJ11100 [Homo sapiens]
dbj|BAA92003.1| (AK001962) unnamed protein product [Homo sapiens]
Length = 129
Score = 195 bits (493), Expect = 5e-49
Identities = 89/131 (67%), Positives = 99/131 (74%), Gaps = 11/131 (8%)
Query: 1 MSAYKRKRGSLPEVATNTKKAKKQLAGSE----QEATAGEEFI-----VPPPVSEGKWKN 51
M+A KRKR A KK K+ +E + ATA E +P PV +GKWKN
Sbjct: 1 MAATKRKRRG--GFAVQAKKPKRNEIDAEPPAKRHATAEEVEEEERDRIPGPVCKGKWKN 58
Query: 52 KERVLIFSSRGINFRTRHLMQDLRSLMPHSRAETKMDRKDKLFVVNEVCEMKNCNKCIYF 111
KER+LIFSSRGINFRTRHLMQDLR LMPHS+A+TKMDRKDKLFV+NEVCEMKNCNKCIYF
Sbjct: 59 KERILIFSSRGINFRTRHLMQDLRMLMPHSKADTKMDRKDKLFVINEVCEMKNCNKCIYF 118
Query: 112 EAKKKQDLYMW 122
EAKKKQDLYMW
Sbjct: 119 EAKKKQDLYMW 129
>emb|CAC18315.1| (AL451022) probable IMP4 protein [Neurospora crassa]
Length = 295
Score = 58.8 bits (141), Expect = 8e-08
Identities = 49/198 (24%), Positives = 88/198 (43%), Gaps = 25/198 (12%)
Query: 54 RVLIFSSRGINFRTRHLMQDLRSLMPHSRAETKMDRKDKLFVVNE----VCEMKNCNKCI 109
R+L+ +SR + R +++R L+P S R ++ +V E + +N +
Sbjct: 85 RILVTTSRDPSSRLGQFSKEIRLLLPTSV------RLNRGNLVLEDLVGAAKAQNLTDVV 138
Query: 110 YFEAKKKQDLYMWLSNSPEGPSAKFLVQNIHTLAELKMSGNCLKG----SRPILSFDPAF 165
+ M +S+ P GP+ + N+ A++ S +KG S P L F+ F
Sbjct: 139 LLHEHRGVPTAMTISHFPHGPTLMVSLHNVVLRADIPKS---IKGTVSESYPRLIFE-GF 194
Query: 166 DREPQYALLKELLTQIFGTPRYHPRSQPFV-DHIFTFSIADNRIWFRNY---QIIEEDAA 221
+ ++K +L +F P P +P V + + TF D+ I R++ + +
Sbjct: 195 STKLGERVVK-ILKHLF--PPREPTQKPNVGNRVITFVNNDDTIEVRHHVYVRTSYDSVE 251
Query: 222 LVEIGPRFVLNLIKIFKG 239
L E+GPRF + KI G
Sbjct: 252 LSEVGPRFTMKPFKITMG 269
>gb|AAF56395.1| (AE003750) CG11920 gene product [Drosophila melanogaster]
Length = 298
Score = 51.8 bits (123), Expect = 1e-05
Identities = 44/213 (20%), Positives = 98/213 (45%), Gaps = 19/213 (8%)
Query: 54 RVLIFSSRGINFRTRHLMQDLRSLMPHSRAETKMDRKD-KLFVVNEVCEMKNCNKCIYFE 112
++++ +S + R + M++LR ++P+++ +M+R + +L + C N +
Sbjct: 82 KIMLTTSHNPSSRLKMFMKELRLIIPNAQ---QMNRGNYQLTTLMHACRANNVTDFLIVH 138
Query: 113 AKKKQDLYMWLSNSPEGPSAKFLVQNIHTLAELKMSGNCLKGSRPILSFDPAFDREPQYA 172
+ + + + P GP+A F + ++ ++ G + +P L F+ F
Sbjct: 139 EHRGIPDSLVVCHLPYGPTAFFNISDVVMRHDIPDIG-HMSEQKPHLIFN-NFKTPIGLR 196
Query: 173 LLKELLTQIFGTPRYHPRSQPFVDHIFTFSIADNRIWFRN--YQIIEEDAALVEIGPRFV 230
+K +L +F P+ + + + +F ++ I FR+ Y+ + ++ L E+GPRF
Sbjct: 197 TVK-ILKHLFPVPKENSQ------RVMSFLNHNDSIIFRHHQYKYVNKELELTEVGPRFS 249
Query: 231 LNLIKIFKGSFG----GPTLYENPHYQSPNMHR 259
L L +I G+ T + N Y + + R
Sbjct: 250 LKLYQIKLGTLENIKAADTEWINRPYMNTSQKR 282
>sp|O13823|YEE7_SCHPO HYPOTHETICAL 33.4 KDA PROTEIN C19A8.07C IN CHROMOSOME I
pir||T37954 hypothetical protein SPAC19A8.07c - fission yeast
(Schizosaccharomyces pombe)
emb|CAB11643.1| (Z98974) hypothetical protein [Schizosaccharomyces pombe]
Length = 289
Score = 47.9 bits (113), Expect = 2e-04
Identities = 51/219 (23%), Positives = 92/219 (41%), Gaps = 23/219 (10%)
Query: 29 EQEATAGEEFIVPPPVSEGKWKNKERVLIFSSRGINFRTRHLMQDLRSLMPHSRAETKMD 88
E AT E + + +VL+ +SR + R +++R L+P+S
Sbjct: 60 ESRATQEETETNLDDEYHRLGEREPKVLVTTSREPSSRLAQFAKEVRLLIPNSY------ 113
Query: 89 RKDKLF-VVNEVCEMKNCNK----CIYFEAKKKQDLYMWLSNSPEGPSAKFLVQNIHTLA 143
R ++ VV + E N I E + D + +S+ P GP+ F + N+
Sbjct: 114 RLNRGNIVVGSLVEAARANDITDIVILHEHRGIPD-GLVISHLPYGPTLSFSLHNVVLRH 172
Query: 144 ELKMSGNCLKGSRPILSFDPAFDREPQYALLKELLTQIFGTPRYHPRSQPFVDHIFTFSI 203
++ +G + + P L F+ + +K L+ +F P + P + TF+
Sbjct: 173 DIPNTGT-MSEAYPHLIFENLTSKLG--KRVKTALSALF--PPDPKDTTP---RVVTFAN 224
Query: 204 ADNRIWFRNY---QIIEEDAALVEIGPRFVLNLIKIFKG 239
D+ I FR++ + + L E GPRF + L +I G
Sbjct: 225 TDDYISFRHHIYAKTGPKQIILSEAGPRFEMKLFEITLG 263
>sp|O62518|YHPK_CAEEL HYPOTHETICAL 34.0 KDA PROTEIN ZK795.3 IN CHROMOSOME IV
pir||T27998 hypothetical protein ZK795.3 - Caenorhabditis elegans
emb|CAB05841.1| (Z83246) predicted using Genefinder~contains similarity to Pfam
domain: PF01945 (Domain of unknown function),
Score=306.9, E-value=7.7e-89, N=1~cDNA EST EMBL:M79771
comes from this gene [Caenorhabditis elegans]
Length = 292
Score = 45.2 bits (106), Expect = 0.001
Identities = 44/193 (22%), Positives = 86/193 (43%), Gaps = 16/193 (8%)
Query: 54 RVLIFSSRGINFRTRHLMQDLRSLMPHSRAETKMDRKDKLFVVNEVCEMKNCNKCIYF-E 112
+++I +SR + R + ++++ + P+++ + + V + + ++ I F E
Sbjct: 79 KIVITTSRDPSSRLKMFAKEMKLIFPNAQRINR--GHYDVKQVVQASKAQDSTDLIIFTE 136
Query: 113 AKKKQDLYMWLSNSPEGPSAKFLVQNIHTLAELKMSGNCLKGSRPILSFDPAFDREPQYA 172
+ D M + + P GP+A F + N+ ++ G + P L FD +
Sbjct: 137 TRGNPD-GMLVCHLPFGPTAFFSMANVVMRHDIPNCGT-MSEQYPHLIFDNLNSKLGHR- 193
Query: 173 LLKELLTQIFGTPRYHPRSQPFVDHIFTFSIADNRIWFRNYQIIEE---DAALVEIGPRF 229
+L +F P+ P S+ I TFS +++ I FR++ E + L E GPRF
Sbjct: 194 -FTTILKHLFPVPK--PDSK----RIITFSNSEDYISFRHHVYKTENDGEVELTEAGPRF 246
Query: 230 VLNLIKIFKGSFG 242
L +I G+
Sbjct: 247 ELKPYQIKLGTLE 259
>ref|NP_014324.1| Imp4p [Saccharomyces cerevisiae]
sp|P53941|IMP4_YEAST U3 SMALL NUCLEOLAR RIBONUCLEOPROTEIN PROTEIN IMP4
pir||S53904 hypothetical protein YNL075w - yeast (Saccharomyces cerevisiae)
emb|CAA60184.1| (X86470) unknown [Saccharomyces cerevisiae]
emb|CAA95949.1| (Z71351) ORF YNL075w [Saccharomyces cerevisiae]
Length = 290
Score = 44.0 bits (103), Expect = 0.003
Identities = 45/218 (20%), Positives = 92/218 (41%), Gaps = 24/218 (11%)
Query: 54 RVLIFSSRGINFRTRHLMQDLRSLMPHSRAETKMDRKD-KLFVVNEVCEMKNCNKCIYFE 112
R+++ +SR + R ++++ L P++ +++R + + + + C+ +
Sbjct: 87 RIIVTTSRDPSTRLSQFAKEIKLLFPNA---VRLNRGNYVMPNLVDACKKSGTTDLVVLH 143
Query: 113 AKKKQDLYMWLSNSPEGPSAKFLVQNIHTLAELKMSGNCLKGSRPILSFDPAFDREPQYA 172
+ + +S+ P GP+A+F + N+ ++ +GN + + P L FD F
Sbjct: 144 EHRGVPTSLTISHFPHGPTAQFSLHNVVMRHDIINAGNQSEVN-PHLIFD-NFTTA---- 197
Query: 173 LLKELLTQIFGTPRYHPRSQPFVD--HIFTFSIADNRIWFRN--YQIIEEDAALVEIGPR 228
L ++ ++ + P D + TF+ + I R Y E + E+GPR
Sbjct: 198 ----LGKRVVCILKHLFNAGPKKDSERVITFANRGDFISVRQHVYVRTREGVEIAEVGPR 253
Query: 229 FVLNLIKIFKGSFGGPTLYENPHYQSPNMHRRMIRLAT 266
F + L ++ G+ EN RR IR A
Sbjct: 254 FEMRLFELRLGTL------ENKDADVEWQLRRFIRTAN 285
>emb|CAC11120.1| (AL357374) bA353C18.4 (novel protein) [Homo sapiens]
Length = 257
Score = 40.9 bits (95), Expect = 0.022
Identities = 28/100 (28%), Positives = 48/100 (48%), Gaps = 9/100 (9%)
Query: 136 VQNIHTLAELKMSGNCLKGSRPILSFD-PAFDREPQYALLKELLTQIFGTPRYHPRSQPF 194
++N +L ++K S C +G++P+L F F+ Y LK LLT F P
Sbjct: 110 IENFVSLKDIKNS-KCPEGTKPMLIFAGDDFNVTEDYRRLKSLLTDFFRGPTVSNICLAG 168
Query: 195 VDHIFTFSIADNRIWFRNYQI-------IEEDAALVEIGP 227
++++ F+ + +I+FR Y++ L EIGP
Sbjct: 169 LEYVLHFTALNGKIYFRRYKLLLKKSGCRTPRIELEEIGP 208
>emb|CAB77726.1| (AL161492) hypothetical protein [Arabidopsis thaliana]
Length = 343
Score = 40.5 bits (94), Expect = 0.028
Identities = 45/204 (22%), Positives = 82/204 (40%), Gaps = 28/204 (13%)
Query: 54 RVLIFSSRGINFRTRHLMQDLRSLMP--HSRAETKMDRKDKLFVVNEVCEMKNCNKCIYF 111
+VL+ + R + R L+ +L S++P H + D K + E K+ I
Sbjct: 128 KVLLTTCRFNSTRGPALISELLSVIPNSHYQKRGTYDLKK----IVEYATKKDFTSLIVV 183
Query: 112 EAKKKQDLYMWLSNSPEGPSAKFLVQNIHTLAELKMSGNCLKGSRPILSFDPAFDREPQY 171
+++ + + P GP+A F + N+ ++K GN +P L + F
Sbjct: 184 HTNRREPDALLIIGLPNGPTAHFKLSNLVLRKDIKNHGNP-TSHQPELVLN-NFTTRLGN 241
Query: 172 ALLKELLTQIFGTPRYHPRSQPFVDHIFTFSIADNRIWFRNYQI------IEEDAA---- 221
+ + Q P + R + + TF + I+FR+++ + D
Sbjct: 242 RVGR--FFQSLFPPDPNFRGR----RVVTFHNQRDFIFFRHHRYIFETKESKSDKGKEET 295
Query: 222 ----LVEIGPRFVLNLIKIFKGSF 241
L E GPRF L L+ + G+F
Sbjct: 296 IKPRLQECGPRFTLKLVTLQHGTF 319
>dbj|BAB14983.1| (AK024740) unnamed protein product [Homo sapiens]
Length = 253
Score = 39.7 bits (92), Expect = 0.052
Identities = 27/100 (27%), Positives = 48/100 (48%), Gaps = 9/100 (9%)
Query: 136 VQNIHTLAELKMSGNCLKGSRPILSFD-PAFDREPQYALLKELLTQIFGTPRYHPRSQPF 194
++N +L ++K S C +G++P+L F FD Y LK LL F P
Sbjct: 121 IENFVSLKDIKNS-KCPEGTKPMLIFAGDDFDVTEDYRRLKSLLIDFFRGPTVSNIRLAG 179
Query: 195 VDHIFTFSIADNRIWFRNYQI-------IEEDAALVEIGP 227
++++ F+ + +I+FR+Y++ L E+GP
Sbjct: 180 LEYVLHFTALNGKIYFRSYKLLLKKSGCRTPRIELEEMGP 219
>gb|AAD14602.1| (AF092910) stage specific peptide 24 [Trypanosoma cruzi]
Length = 287
Score = 39.3 bits (91), Expect = 0.060
Identities = 38/209 (18%), Positives = 82/209 (39%), Gaps = 30/209 (14%)
Query: 45 SEGKWKNKERVLIFSSRGINFRTRHLMQDLRSLMPHSRAETK--------MDRKDKLFVV 96
RVL+ +SR + + +++R ++P + + MD +
Sbjct: 75 YANAGVEDPRVLVTTSREPSQKLLEFAKEIRLVIPSAVRMNRGNLSVRQLMDAARRGQ-- 132
Query: 97 NEVCEMKNCNKCIYFEAKKKQDLYMWLSNSPEGPSAKFLVQNIHTLAELKMSGNCLKGSR 156
+ + E++ D + +S+ P GP+ F + N+ T +++ G +
Sbjct: 133 -------YSDVVVLQESQGVPDS-LTVSHLPLGPTVVFTIHNLVTRHDIQDVGT-MSEQH 183
Query: 157 PILSFDPAFDREPQYALLKELLTQIFGTPRYHPRSQPFVDHIFTFSIADNRIWFRNYQII 216
P L F+ F ++++L +F P +P + TF ++ + FR++
Sbjct: 184 PHLIFE-NFTTRLGRR-VRDVLKFLFPVP------KPRPTRVLTFDNQNDFVSFRHHTFR 235
Query: 217 E---EDAALVEIGPRFVLNLIKIFKGSFG 242
+ L E+GPR + +I G+
Sbjct: 236 AVKGREVQLTEVGPRMDVAPYRITLGTLE 264
>gb|AAG52427.1|AC011622_15 (AC011622) putative U3 small nucleolar ribonucleoprotein protein;
1537-3735 [Arabidopsis thaliana]
gb|AAG52449.1|AC010852_6 (AC010852) putative U3 small nucleolar ribonucleoprotein protein;
73469-75667 [Arabidopsis thaliana]
Length = 294
Score = 39.3 bits (91), Expect = 0.063
Identities = 53/229 (23%), Positives = 99/229 (43%), Gaps = 36/229 (15%)
Query: 17 NTKKAKKQLAGSEQEATAGEEFIVPPPVSEGKWKNKE----RVLIFSSRGINFRTRHLMQ 72
N + +Q E + TA VP + ++ N ++L+ +SR + +
Sbjct: 48 NVEAKLRQEIDLEDQNTA-----VPRSHIDDEYANATEADPKILLTTSRNPSAPLIRFTK 102
Query: 73 DLRSLMPHSRAETKMDRKDKLFVVNEVCEMKNCNK----CIYFEAKKKQDLYMWLSNSPE 128
+L+ + P+S+ +++R V++E+ E + + E + D + +S+ P
Sbjct: 103 ELKFVFPNSQ---RINRGS--QVISEIIETARSHDFTDVILVHEHRGVPD-GLIISHLPF 156
Query: 129 GPSAKFLVQNI---HTLAELKMSGNCLKGSRPILSFDPAFDREPQYALLKELLTQIFGTP 185
GP+A F + N+ H +++ K G + P L F+ F + + +L IF P
Sbjct: 157 GPTAYFGLLNVVTRHDISDKKSIGK-MPEQYPHLIFN-NFTTQMG-QRVGNILKHIFPAP 213
Query: 186 RYHPRSQPFVDHIFTFSIADNRIWFRNY-----QIIEEDAALVEIGPRF 229
+ + I TFS + I FRN+ + + L EIGPRF
Sbjct: 214 KLDAK------RIVTFSNQSDYISFRNHVYDKGEGGPKSIELKEIGPRF 256
>pir||T39008 probable mating protein - fission yeast (Schizosaccharomyces pombe)
emb|CAB11063.1| (Z98531) putative mating protein [Schizosaccharomyces pombe]
Length = 361
Score = 39.0 bits (90), Expect = 0.087
Identities = 16/42 (38%), Positives = 24/42 (57%)
Query: 220 AALVEIGPRFVLNLIKIFKGSFGGPTLYENPHYQSPNMHRRM 261
L+EIGPR L LIKI + + GG LY + ++S ++
Sbjct: 259 IKLIEIGPRMTLELIKITEDAMGGKVLYHSHVHKSKEEIKQQ 300
>pir||T50616 hypothetical protein DKFZp761G0415.1 - human (fragment)
Length = 256
Score = 39.0 bits (90), Expect = 0.093
Identities = 53/242 (21%), Positives = 95/242 (38%), Gaps = 39/242 (16%)
Query: 19 KKAKKQLAGSEQEATAGEEFIVPPPVSEG--KWKNKE---RVLIFSSRGINFRTRHLMQD 73
K Q E +E + ++ + NK+ ++LI +S + RT L +
Sbjct: 10 KTIDNQRVYDETTVDPNDEEVAYDEATDEFASYFNKQTSPKILITTSDRPHGRTVRLCEQ 69
Query: 74 LRSLMPHSRAETKMDRKDKLFVVNEVCEMKNCNKCIYFEAKKKQDLYMWLSNSPEGPSAK 133
L +++P+S + R L + C ++ I +K + LS+ P GP+A
Sbjct: 70 LSTVIPNSHVYYR--RGLALKKIIPQCIARDFTDLIVINEDRKTPNGLILSHLPNGPTAH 127
Query: 134 FLVQNIHTLAELKMSGNCLKGSRPILSFDPAFDREPQYALLKELLTQIFGTPRYH----- 188
F + ++ E+K G D + E++ F T H
Sbjct: 128 FKMSSVRLRKEIKRRGK---------------DPTEH---IPEIILNNFTTRLGHSIGRM 169
Query: 189 -----PRSQPFVDH-IFTFSIADNRIWFRNYQI---IEEDAALVEIGPRFVLNLIKIFKG 239
P + F+ + TF + I+FR ++ E+ + E+GPRF L L + KG
Sbjct: 170 FASLFPHNPQFIGRQVATFHNQRDYIFFRFHRYIFRSEKKVGIQELGPRFTLKLRSLQKG 229
Query: 240 SF 241
+F
Sbjct: 230 TF 231
>pir||T01938 hypothetical protein F11O4.6 - Arabidopsis thaliana
gb|AAC62782.1| (AF096370) contains similarity to a C. elegans hypothetical protein
F44G4.1 (GB:Z49910) and several yeast hypothetical
proteins such as 35.1 KD protein in NAM8-GAR1 intergenic
region (SP:P38805) [Arabidopsis thaliana]
Length = 434
Score = 38.2 bits (88), Expect = 0.16
Identities = 51/259 (19%), Positives = 96/259 (36%), Gaps = 49/259 (18%)
Query: 20 KAKKQLAGSEQEATAGEEFIVPPPVSEGKWKNKE--------------RVLIFSSRGINF 65
K +++ E T + V P E + + + +VL+ + R +
Sbjct: 93 KPPQKMIPKTIENTRESDETVCRPDDEELFADIDADEFNPVLRREIAPKVLLTTCRFNST 152
Query: 66 RTRHLMQDLRSLMP--HSRAETKMDRKDKLFVVNEVCEMKNCNKCIYFEAKKKQDLY--- 120
R L+ +L S++P H + D K + E K+ I +++ +
Sbjct: 153 RGPALISELLSVIPNSHYQKRGTYDLKK----IVEYATKKDFTSLIVVHTNRREPAFAIS 208
Query: 121 ----MWLSNSPEGPSAKFLVQNIHTLAELKMSGNCLKGSRPILSFDPAFDREPQYALLKE 176
+ + P GP+A F + N+ ++K GN +P L + F + +
Sbjct: 209 YVDALLIIGLPNGPTAHFKLSNLVLRKDIKNHGNP-TSHQPELVLN-NFTTRLGNRVGR- 265
Query: 177 LLTQIFGTPRYHPRSQPFVDHIFTFSIADNRIWFRNYQI------IEEDAA--------L 222
Q P + R + + TF + I+FR+++ + D L
Sbjct: 266 -FFQSLFPPDPNFRGR----RVVTFHNQRDFIFFRHHRYIFETKESKSDKGKEETIKPRL 320
Query: 223 VEIGPRFVLNLIKIFKGSF 241
E GPRF L L+ + G+F
Sbjct: 321 QECGPRFTLKLVTLQHGTF 339
>gb|AAF53162.1| (AE003635) CG6712 gene product [Drosophila melanogaster]
Length = 394
Score = 37.4 bits (86), Expect = 0.22
Identities = 41/196 (20%), Positives = 83/196 (41%), Gaps = 13/196 (6%)
Query: 54 RVLIFSSRGINFRTRHLMQDLRSLMPHSRAETKMDRKDKLFVVNEVCEMKNCNKCIYFEA 113
+VLI + +TR +L + P++ K+ K + + + E + +
Sbjct: 186 KVLITFADNPVTKTRKFGLELSRIFPNAL--VKIRNKSSVKKICKSAEREEFTDVVIVNE 243
Query: 114 KKKQDLYMWLSNSPEGPSAKFLVQNIHTLAELKMSGNCLKGSRPILSFDPAFDREPQYAL 173
+++ + + + P GP+A F + N+ +++K + RP + + F
Sbjct: 244 DRRKPNGLLVIHLPNGPTAHFKLSNVKLTSDIKRDHKEITKHRPEVILN-NFTTRLGLT- 301
Query: 174 LKELLTQIFGTPRYHPRSQPFVDHIFTFSIADNRIWFRN--YQIIEEDA--ALVEIGPRF 229
+ +L +F + P + TF + I+FR+ Y+ +E L E+GPRF
Sbjct: 302 VGRMLGALF---HHDPEFRG--RRAVTFHNQRDYIFFRHHRYEFTKEGKRVKLRELGPRF 356
Query: 230 VLNLIKIFKGSFGGPT 245
L L + +G+F T
Sbjct: 357 TLKLRSLQEGTFDSKT 372
>dbj|BAB14086.1| (AK022537) unnamed protein product [Homo sapiens]
Length = 349
Score = 36.6 bits (84), Expect = 0.39
Identities = 57/259 (22%), Positives = 103/259 (39%), Gaps = 42/259 (16%)
Query: 5 KRKRGSLPEVATNTKKAK---KQLAGSEQEATAGEEFIVPPPVSEG--KWKNKE---RVL 56
K++R +L + A K Q E +E + ++ + NK+ ++L
Sbjct: 86 KKEREALGDKAPPKPVPKTIDNQRVYDETTVDPNDEEVAYDEATDEFASYFNKQTSPKIL 145
Query: 57 IFSSRGINFRTRHLMQDLRSLMPHSRAETKMDRKDKLFVVNEVCEMKNCNKCIYFEAKKK 116
I +S + RT L + L +++P+S + R L + C ++ I +K
Sbjct: 146 ITTSDRPHGRTVRLCEQLSTVIPNSHVYYR--RGLALKKIIPQCIARDFTDLIVINEDRK 203
Query: 117 QDLYMWLSNSPEGPSAKFLVQNIHTLAELKMSGNCLKGSRPILSFDPAFDREPQYALLKE 176
+ LS+ P GP+A F + ++ E+K G D + E
Sbjct: 204 TPNGLILSHLPNGPTAHFKMSSVRLRKEIKRRGK---------------DPTEH---IPE 245
Query: 177 LLTQIFGTPRYH----------PRSQPFVDH-IFTFSIADNRIWFRNYQI---IEEDAAL 222
++ F T H P + F+ + TF + I+FR ++ E+ +
Sbjct: 246 IILNNFTTRLGHSIGRMFASLFPHNPQFIGRQVATFHNQRDYIFFRFHRYIFRSEKKVGI 305
Query: 223 VEIGPRFVLNLIKIFKGSF 241
E+GPRF L L + KG+F
Sbjct: 306 QELGPRFTLKLRSLQKGTF 324
>gb|AAD02810.1| (AF062396) protein phosphatase 2A regulatory subunit isoform B'
delta [Arabidopsis thaliana]
dbj|BAB01066.1| (AB023041) protein phosphatase 2A regulatory subunit B'
[Arabidopsis thaliana]
Length = 477
Score = 36.6 bits (84), Expect = 0.40
Identities = 11/38 (28%), Positives = 20/38 (51%), Gaps = 1/38 (2%)
Query: 169 PQYALLKELLTQIFGTPRYHPR-SQPFVDHIFTFSIAD 205
P ++ E L +I +P P+ S+ ++DH F + D
Sbjct: 167 PHLQIVYEFLLRIVASPNTDPKISKKYIDHTFVLKLLD 204
>gb|AAD16459.1| (AF102805) Peter Pan [Drosophila melanogaster]
Length = 460
Score = 35.8 bits (82), Expect = 0.66
Identities = 15/36 (41%), Positives = 19/36 (52%)
Query: 212 NYQIIEEDAALVEIGPRFVLNLIKIFKGSFGGPTLY 247
N + + L EIGPR + LIKI +G G LY
Sbjct: 260 NLEDKKSSIKLHEIGPRLTMQLIKIEEGLLTGEVLY 295
>gb|AAF55851.1| (AE003733) ppan gene product [Drosophila melanogaster]
Length = 460
Score = 35.8 bits (82), Expect = 0.75
Identities = 15/36 (41%), Positives = 19/36 (52%)
Query: 212 NYQIIEEDAALVEIGPRFVLNLIKIFKGSFGGPTLY 247
N + + L EIGPR + LIKI +G G LY
Sbjct: 260 NLEDKKSSIKLHEIGPRLTMQLIKIEEGLLTGEVLY 295
>sp|O14180|YDS4_SCHPO HYPOTHETICAL 35.8 KD PROTEIN C4F8.04 IN CHROMOSOME I
pir||T38834 hypothetical coiled-coil protein - fission yeast
(Schizosaccharomyces pombe)
emb|CAB11051.1| (Z98530) hypothetical coiled-coil protein [Schizosaccharomyces
pombe]
Length = 306
Score = 33.9 bits (77), Expect = 2.6
Identities = 41/209 (19%), Positives = 78/209 (36%), Gaps = 26/209 (12%)
Query: 44 VSEGKWKNKERVLIFSSRGINFRTRHLMQDLRSLMPHSRAETKMDRKDKLFV-VNEVCEM 102
+ + ++L+ +S+ + + +L P++ RK + V+E+ E
Sbjct: 85 AYFSEERKVPKLLVTTSKRASRKCYDFASELLDCFPNAEF-----RKRTGDIEVHEIAEA 139
Query: 103 ---KNCNKCIYFEAKKKQDLYMWLSNSPEGPSAKFLVQNIHTLAELKMSGNCLKGSRPIL 159
+ + +K+ + L + P GPS F + N+ T E+ G G P L
Sbjct: 140 AAKRGYTDLLVLNEDRKKTNALTLVHLPNGPSFYFTLSNLQTAKEISNHGRS-TGHIPEL 198
Query: 160 SFDPAFDREPQYALLKELLTQIFGTPRYHPRSQPFVDHIFTFSIADNRIWFR--NYQIIE 217
+ F + + + TP+ R + T + ++FR Y E
Sbjct: 199 IIN-NFSTRLGMTVARAFQSLFIQTPQIQGR------QVVTIHCQRDFLFFRRHRYAFRE 251
Query: 218 ED-------AALVEIGPRFVLNLIKIFKG 239
+ L E+GPRF + L + KG
Sbjct: 252 KSNMPDGIGTGLQELGPRFTMRLRMVQKG 280
>gb|AAG52369.1|AC011765_21 (AC011765) putative cytochrome P450; 69682-71175 [Arabidopsis
thaliana]
Length = 497
Score = 33.9 bits (77), Expect = 2.6
Identities = 12/24 (50%), Positives = 15/24 (62%)
Query: 122 WLSNSPEGPSAKFLVQNIHTLAEL 145
W SN P GP KFL+ N+H + L
Sbjct: 23 WRSNIPPGPKPKFLLGNLHQMKPL 46
>sp|P07788|COTA_BACSU SPORE COAT PROTEIN A
pir||F69604 spore coat protein (outer) cotA - Bacillus subtilis
emb|CAB12449.1| (Z99107) spore coat protein (outer) [Bacillus subtilis]
Length = 513
Score = 33.5 bits (76), Expect = 4.0
Identities = 24/94 (25%), Positives = 39/94 (40%), Gaps = 9/94 (9%)
Query: 136 VQNIHTLAELKMSGNCLKGSRPILSFDPAFDREPQYALLKELLTQIFGTPRYHPRSQPFV 195
+QNI T LK++G + RP+L + +P K T+I+ + P
Sbjct: 366 IQNIRT---LKLAGTQDEYGRPVLLLNNKRWHDPVTETPKVGTTEIWSIINPTRGTHPIH 422
Query: 196 DHIFTFSIADNRIWFRNYQII--EEDAALVEIGP 227
H+ +F + D R + I +E L GP
Sbjct: 423 LHLVSFRVLDR----RPFDIARYQESGELSYTGP 452
>dbj|BAA22774.1| (AB007638) spore coat proein A [Bacillus subtilis]
Length = 515
Score = 33.5 bits (76), Expect = 4.0
Identities = 24/94 (25%), Positives = 39/94 (40%), Gaps = 9/94 (9%)
Query: 136 VQNIHTLAELKMSGNCLKGSRPILSFDPAFDREPQYALLKELLTQIFGTPRYHPRSQPFV 195
+QNI T LK++G + RP+L + +P K T+I+ + P
Sbjct: 368 IQNIRT---LKLAGTQDEYGRPVLLLNNKRWHDPVTETPKVGTTEIWSIINPTRGTHPIH 424
Query: 196 DHIFTFSIADNRIWFRNYQII--EEDAALVEIGP 227
H+ +F + D R + I +E L GP
Sbjct: 425 LHLVSFRVLDR----RPFDIARYQESGELSYTGP 454
>pir||T32923 hypothetical protein K09H9.6 - Caenorhabditis elegans
gb|AAB97575.1| (AF043700) contains similarity to human RNA-binding protein FUS/TLS
(SW:Q28009) [Caenorhabditis elegans]
Length = 608
Score = 33.1 bits (75), Expect = 5.4
Identities = 15/39 (38%), Positives = 22/39 (55%)
Query: 218 EDAALVEIGPRFVLNLIKIFKGSFGGPTLYENPHYQSPN 256
+ L EIGPR L L+KI +G G LY + ++P+
Sbjct: 336 SNVRLHEIGPRLTLELVKIEEGIDEGEVLYHKHNAKTPD 374
CPU time: 38.87 user secs. 1.51 sys. secs 40.38 total secs.
Database: nr
Posted date: Feb 10, 2001 7:10 PM
Number of letters in database: 195,544,254
Number of sequences in database: 618,844
Lambda K H
0.312 0.159 0.497
Gapped
Lambda K H
0.270 0.0558 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 109967341
Number of Sequences: 618844
Number of extensions: 4278597
Number of successful extensions: 11771
Number of sequences better than 10.0: 82
Number of HSP's better than 10.0 without gapping: 16
Number of HSP's successfully gapped in prelim test: 66
Number of HSP's that attempted gapping in prelim test: 11695
Number of HSP's gapped (non-prelim): 101
length of query: 339
length of database: 195,544,254
effective HSP length: 56
effective length of query: 283
effective length of database: 160,888,990
effective search space: 45531584170
effective search space used: 45531584170
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.1 bits)
S2: 72 (32.2 bits)