BLAST Search Results

BLASTP 2.1.1 [Aug-8-2000]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|2496063|sp|Q58012|Y594_METJA HYPOTHETICAL PROTEIN
MJ0594 gi|2128398|pir||B64374 hypothetical protein
MJ0594|gi|1591303|gb|AAB98595.1| (U67508) M. jannaschii predicted
coding region MJ0594 [Methanococcus jannaschii]
         (169 letters)

Database: nr
           618,844 sequences; 195,544,254 total letters

Searching..................................................

E-value threshold for inclusion in PSI-Blast iteration 1: 0.002 
E-value threshold for inclusion in PSI-Blast iteration 2:

Distribution of 9 Blast Hits on the Query Sequence


Sequences with E-value BETTER than threshold 
                                                                     Score    E
Sequences producing significant alignments:                          (bits) Value

 sp|Q58012|Y594_METJA  HYPOTHETICAL PROTEIN MJ0594 >gi|2128398|...   302  1e-81
 pir||F69190  conserved hypothetical protein MTH680 - Methanoba...    59  3e-08
 ref|NP_069935.1|  conserved hypothetical protein [Archaeoglobu...    58  6e-08
 pir||B72623  hypothetical protein APE1443 - Aeropyrum pernix (...    54  1e-06
 pir||H71203  hypothetical protein PH1900 - Pyrococcus horikosh...    47  1e-04
 pir||G75218  hypothetical protein PAB2357 - Pyrococcus abyssi ...    45  4e-04

 
Sequences with E-value WORSE than threshold 
 emb|CAB57572.1|  (Y18930) hypothetical protein [Sulfolobus sol...    35  0.61
 sp|O62518|YHPK_CAEEL  HYPOTHETICAL 34.0 KDA PROTEIN ZK795.3 IN...    33  2.3
 ref|NP_014227.1|  subunit of ubiquitin- protein ligase; Apc1p ...    32  4.3


Alignments
>sp|Q58012|Y594_METJA HYPOTHETICAL PROTEIN MJ0594
 pir||B64374 hypothetical protein MJ0594 - Methanococcus jannaschii
 gb|AAB98595.1| (U67508) M. jannaschii predicted coding region MJ0594
           [Methanococcus jannaschii]
          Length = 169

 Score =  302 bits (767), Expect = 1e-81
 Identities = 169/169 (100%), Positives = 169/169 (100%)

Query: 1   MILTTSRKPSQRTRSFARDLERTLNIPYVQRGKLSLKEIFEIDKHVLLIGEFKANPGTLV 60
           MILTTSRKPSQRTRSFARDLERTLNIPYVQRGKLSLKEIFEIDKHVLLIGEFKANPGTLV
Sbjct: 1   MILTTSRKPSQRTRSFARDLERTLNIPYVQRGKLSLKEIFEIDKHVLLIGEFKANPGTLV 60

Query: 61  VYDVENEKRLSSFISVKLQREICGEKIYNDDGIRIKISRELKDNEEFQKYYEIYDEFLFQ 120
           VYDVENEKRLSSFISVKLQREICGEKIYNDDGIRIKISRELKDNEEFQKYYEIYDEFLFQ
Sbjct: 61  VYDVENEKRLSSFISVKLQREICGEKIYNDDGIRIKISRELKDNEEFQKYYEIYDEFLFQ 120

Query: 121 HLNINEDSDITLRLEKDPKYLFAIQFYKGRVKIGPLIRIKSIKLFDSLL 169
           HLNINEDSDITLRLEKDPKYLFAIQFYKGRVKIGPLIRIKSIKLFDSLL
Sbjct: 121 HLNINEDSDITLRLEKDPKYLFAIQFYKGRVKIGPLIRIKSIKLFDSLL 169



>pir||F69190 conserved hypothetical protein MTH680 - Methanobacterium
          thermoautotrophicum (strain Delta H)
 gb|AAB85185.1| (AE000848) conserved protein [Methanothermobacter
          thermautotrophicus]
          Length = 155

 Score = 59.1 bits (141), Expect = 3e-08
 Identities = 30/70 (42%), Positives = 43/70 (60%), Gaps = 1/70 (1%)

Query: 1  MILTTSRKPSQRTRSFARDLERTLNIPYVQRGKLSLKEIF-EIDKHVLLIGEFKANPGTL 59
          M+LTTSRKPSQRTRSF++ L R +   Y+ RGK+SL+++  E    V ++ E   NP  +
Sbjct: 1  MLLTTSRKPSQRTRSFSQRLSRIMGWRYINRGKMSLRDVLIEARGPVAVVSERHGNPARI 60

Query: 60 VVYDVENEKR 69
             D    +R
Sbjct: 61 TFLDERGGER 70



>ref|NP_069935.1| conserved hypothetical protein [Archaeoglobus fulgidus]
 pir||A69388 conserved hypothetical protein AF1106 - Archaeoglobus fulgidus
 gb|AAB90132.1| (AE001027) conserved hypothetical protein [Archaeoglobus
          fulgidus]
          Length = 153

 Score = 57.9 bits (138), Expect = 6e-08
 Identities = 30/62 (48%), Positives = 41/62 (65%)

Query: 2  ILTTSRKPSQRTRSFARDLERTLNIPYVQRGKLSLKEIFEIDKHVLLIGEFKANPGTLVV 61
          +LTTSRKP ++TR FA+ L R  N  YV RGKLSL+++  I +   +I E K NP  L +
Sbjct: 3  VLTTSRKPGRKTRRFAKVLARFFNWKYVNRGKLSLEDLAGIAERFWIISEVKGNPAILNL 62

Query: 62 YD 63
          Y+
Sbjct: 63 YE 64



>pir||B72623 hypothetical protein APE1443 - Aeropyrum pernix (strain K1)
 dbj|BAA80440.1| (AP000061) 197aa long hypothetical protein [Aeropyrum pernix]
          Length = 197

 Score = 54.0 bits (128), Expect = 1e-06
 Identities = 33/92 (35%), Positives = 51/92 (54%), Gaps = 11/92 (11%)

Query: 1   MILTTSRKPSQRTRSFARDLERTLNIPY-VQRGKLSLKE-----IFEIDKHVLLIGEFKA 54
           +++TTSR+PS R RSF +DL  T+   +   RG  S++E     I      ++++GE + 
Sbjct: 16  ILVTTSRRPSPRIRSFVKDLSATIPGAFRFTRGHYSMEELAREAIIRGADRIVVVGERRG 75

Query: 55  NPGTLVVYDVENEKRLSSFIS-----VKLQRE 81
           NPG + VY VE  +R  + +S     V L RE
Sbjct: 76  NPGIIRVYAVEGPERPDNIVSFIVKGVSLSRE 107



>pir||H71203 hypothetical protein PH1900 - Pyrococcus horikoshii
 dbj|BAA31023.1| (AP000007) 334aa long hypothetical protein [Pyrococcus
          horikoshii]
          Length = 224

 Score = 47.0 bits (110), Expect = 1e-04
 Identities = 34/92 (36%), Positives = 51/92 (54%), Gaps = 10/92 (10%)

Query: 1  MILTTSRKPSQRTRSFARDLERTL-NIPYVQRGKLSLKEIF--EIDK---HVLLIGEFKA 54
          M++TTS +P++RTRSF  DLER + N  Y+ RGK +++E+     D+    +L+I  +K 
Sbjct: 2  MLITTSHRPTRRTRSFGHDLERVIPNSLYLTRGKKTIQELLMEAYDRGYERLLIINVWKG 61

Query: 55 NPGTLVVYDVENEK----RLSSFISVKLQREI 82
          NP  +    V  E            VKLQRE+
Sbjct: 62 NPLKMTFIKVHPEDWGYLGYLYLHGVKLQREM 93



>pir||G75218 hypothetical protein PAB2357 - Pyrococcus abyssi (strain Orsay)
 emb|CAB49198.1| (AJ248283) hypothetical protein [Pyrococcus abyssi]
          Length = 224

 Score = 45.4 bits (106), Expect = 4e-04
 Identities = 33/92 (35%), Positives = 50/92 (53%), Gaps = 10/92 (10%)

Query: 1  MILTTSRKPSQRTRSFARDLERTL-NIPYVQRGKLSLKEIF--EIDK---HVLLIGEFKA 54
          M++TTS +P++RTRSF  DLER   N  Y+ RGK +++E+     D+    +L+I  +K 
Sbjct: 2  MLITTSHRPTRRTRSFGHDLERVFPNSLYMTRGKKTIQELLMEAYDRGYERLLIINVWKG 61

Query: 55 NPGTLVVYDVENEK----RLSSFISVKLQREI 82
          NP  +    V  +            VKLQRE+
Sbjct: 62 NPLKMTFIKVHPDDWGYLGYLYLHGVKLQREM 93



>emb|CAB57572.1| (Y18930) hypothetical protein [Sulfolobus solfataricus]
 emb|CAC23146.1| (AL512964) hypothetical [Sulfolobus solfataricus]
          Length = 170

 Score = 34.9 bits (79), Expect = 0.61
 Identities = 26/72 (36%), Positives = 40/72 (55%), Gaps = 6/72 (8%)

Query: 1  MILTTSRKPSQRTRSFARDLERTL-NIPYVQRGKLSLKEIFEI-----DKHVLLIGEFKA 54
          +++T+SR PS RTR+F   L   L +   + RGK S  EIFE        ++L +     
Sbjct: 8  IVITSSRDPSIRTRNFLNVLTFVLPDSVKITRGKKSKIEIFERAINLGALYLLFVLAKNG 67

Query: 55 NPGTLVVYDVEN 66
          NP  ++VYD+E+
Sbjct: 68 NPLRIIVYDLES 79



>sp|O62518|YHPK_CAEEL HYPOTHETICAL 34.0 KDA PROTEIN ZK795.3 IN CHROMOSOME IV
 pir||T27998 hypothetical protein ZK795.3 - Caenorhabditis elegans
 emb|CAB05841.1| (Z83246) predicted using Genefinder~contains similarity to Pfam
           domain: PF01945 (Domain of unknown function),
           Score=306.9, E-value=7.7e-89, N=1~cDNA EST EMBL:M79771
           comes from this gene [Caenorhabditis elegans]
          Length = 292

 Score = 33.0 bits (74), Expect = 2.3
 Identities = 18/67 (26%), Positives = 37/67 (54%), Gaps = 6/67 (8%)

Query: 1   MILTTSRKPSQRTRSFARDLERTL-NIPYVQRGKLSLKEIFEIDK-----HVLLIGEFKA 54
           +++TTSR PS R + FA++++    N   + RG   +K++ +  K      +++  E + 
Sbjct: 80  IVITTSRDPSSRLKMFAKEMKLIFPNAQRINRGHYDVKQVVQASKAQDSTDLIIFTETRG 139

Query: 55  NPGTLVV 61
           NP  ++V
Sbjct: 140 NPDGMLV 146



>ref|NP_014227.1| subunit of ubiquitin- protein ligase; Apc1p [Saccharomyces
           cerevisiae]
 sp|P53886|YNR2_YEAST HYPOTHETICAL 196.1 KD PROTEIN IN RPS3-PSD1 INTERGENIC REGION
 pir||S63127 probable membrane protein YNL172w - yeast (Saccharomyces
           cerevisiae)
 emb|CAA96060.1| (Z71448) ORF YNL172w [Saccharomyces cerevisiae]
          Length = 1748

 Score = 32.2 bits (72), Expect = 4.3
 Identities = 15/27 (55%), Positives = 21/27 (77%), Gaps = 2/27 (7%)

Query: 105 EEFQKYYEIYDEFLFQHLNINEDSDIT 131
           +E+Q+Y EIY+  LFQHL   +DS+IT
Sbjct: 596 QEYQEYKEIYERDLFQHL--KQDSEIT 620



CPU time:    24.16 user secs.	    0.51 sys. secs	   24.67 total secs.

  Database: nr
    Posted date:  Feb 10, 2001  7:10 PM
  Number of letters in database: 195,544,254
  Number of sequences in database:  618,844
  
Lambda     K      H
   0.322    0.143    0.395 

Gapped
Lambda     K      H
   0.270   0.0470    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 60539884
Number of Sequences: 618844
Number of extensions: 2467118
Number of successful extensions: 6119
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 13
Number of HSP's that attempted gapping in prelim test: 6108
Number of HSP's gapped (non-prelim): 23
length of query: 169
length of database: 195,544,254
effective HSP length: 54
effective length of query: 115
effective length of database: 162,126,678
effective search space: 18644567970
effective search space used: 18644567970
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.8 bits)
S2: 68 (30.9 bits)