Sequences with E-value BETTER than threshold 230 7e-60
49 5e-05
44 0.001
44 0.001
Sequences with E-value WORSE than threshold ref|NP_011956.1| Rpf1p [Saccharomyces cerevisiae] >gi|731684|... 42 0.006
dbj|BAB14086.1| (AK022537) unnamed protein product [Homo sapi... 41 0.012
emb|CAB55338.1| (AJ006754) hypothetical protein [Yarrowia lip... 40 0.023
pir||T50616 hypothetical protein DKFZp761G0415.1 - human (fra... 40 0.025
sp|O14180|YDS4_SCHPO HYPOTHETICAL 35.8 KD PROTEIN C4F8.04 IN ... 38 0.065
gb|AAG38541.1|AF309805_6 (AF309805) coiled-coil protein [Pneu... 37 0.17
emb|CAB77726.1| (AL161492) hypothetical protein [Arabidopsis ... 36 0.40
emb|CAB77655.1| (AJ390518) hypothetical protein [Candida albi... 35 0.92
pir||T01938 hypothetical protein F11O4.6 - Arabidopsis thalia... 32 4.7
gb|AAD14602.1| (AF092910) stage specific peptide 24 [Trypanos... 32 6.3
Alignments
>emb|CAC27105.1| (AJ010592) hypothetical protein [Guillardia theta]
Length = 186
Score = 230 bits (582), Expect = 7e-60
Identities = 160/186 (86%), Positives = 160/186 (86%)
Query: 1 MKFYXXXXXXXXXXXXXXXXXXXXXXXXXXPRYYKRNKFKFRHLMIYLKRKNINNMIYLF 60
MKFY PRYYKRNKFKFRHLMIYLKRKNINNMIYLF
Sbjct: 1 MKFYSIITTSKKPSKTLLKILHLLKKYLITPRYYKRNKFKFRHLMIYLKRKNINNMIYLF 60
Query: 61 ENNNRYFVSIVNFQENIHIKFGINNLLITSRISSIIYKDYPEIIYLNFKAQHEIYIQKVL 120
ENNNRYFVSIVNFQENIHIKFGINNLLITSRISSIIYKDYPEIIYLNFKAQHEIYIQKVL
Sbjct: 61 ENNNRYFVSIVNFQENIHIKFGINNLLITSRISSIIYKDYPEIIYLNFKAQHEIYIQKVL 120
Query: 121 TQILYQVSPLTNRELLCIYSNKGIIYFRSFRYIFSKNLRDVKIQEIGPRLNFKILNILSF 180
TQILYQVSPLTNRELLCIYSNKGIIYFRSFRYIFSKNLRDVKIQEIGPRLNFKILNILSF
Sbjct: 121 TQILYQVSPLTNRELLCIYSNKGIIYFRSFRYIFSKNLRDVKIQEIGPRLNFKILNILSF 180
Query: 181 KINKSI 186
KINKSI
Sbjct: 181 KINKSI 186
>gb|AAF53162.1| (AE003635) CG6712 gene product [Drosophila melanogaster]
Length = 394
Score = 48.9 bits (115), Expect = 5e-05
Identities = 33/143 (23%), Positives = 70/143 (48%), Gaps = 4/143 (2%)
Query: 36 RNKFKFRHLMIYLKRKNINNMIYLFENNNR-YFVSIVNFQENIHIKFGINNLLITSRIS- 93
RNK + + +R+ +++ + E+ + + +++ F ++N+ +TS I
Sbjct: 218 RNKSSVKKICKSAEREEFTDVVIVNEDRRKPNGLLVIHLPNGPTAHFKLSNVKLTSDIKR 277
Query: 94 --SIIYKDYPEIIYLNFKAQHEIYIQKVLTQILYQVSPLTNRELLCIYSNKGIIYFRSFR 151
I K PE+I NF + + + ++L + + R + ++ + I+FR R
Sbjct: 278 DHKEITKHRPEVILNNFTTRLGLTVGRMLGALFHHDPEFRGRRAVTFHNQRDYIFFRHHR 337
Query: 152 YIFSKNLRDVKIQEIGPRLNFKI 174
Y F+K + VK++E+GPR K+
Sbjct: 338 YEFTKEGKRVKLRELGPRFTLKL 360
>sp|P54073|YUY1_CAEEL HYPOTHETICAL 87.9 KDA PROTEIN F44G4.1 IN CHROMOSOME II PRECURSOR
Length = 754
Score = 43.9 bits (102), Expect = 0.001
Identities = 33/140 (23%), Positives = 65/140 (45%), Gaps = 9/140 (6%)
Query: 44 LMIYLKRKNINNMIY--LFENNNRYF-VSIVNFQENIHIKFGINNLLITSRISSIIYK-- 98
L+++ RK N +I+ L E YF ++ + F +++ + + N + + + YK
Sbjct: 582 LVVHEDRKKPNGIIFCHLPEGPTAYFKINSLTFTQDLKVCYFDNFFMYCLKSLKLFYKFG 641
Query: 99 ----DYPEIIYLNFKAQHEIYIQKVLTQILYQVSPLTNRELLCIYSNKGIIYFRSFRYIF 154
+PE+I NF + I ++L + T R ++ ++ + I+FR RY F
Sbjct: 642 ESTSHFPEVILNNFNTRLGHNIARMLACLFPHDPKFTGRRVVTFHNQRDYIFFRHHRYEF 701
Query: 155 SKNLRDVKIQEIGPRLNFKI 174
K + E+GPR ++
Sbjct: 702 KKEGSKAALLELGPRFTLRL 721
>pir||T19409 hypothetical protein F44G4.1 - Caenorhabditis elegans
emb|CAA93858.2| (Z70034) similarity to 35.1KD hypothetical yeast protein (Swiss
Prot accession number P38805), contains similarity to
Pfam domain: PF01945 (Domain of unknown function),
Score=96.8, E-value=1.3e-25, N=1~cDNA EST CEMSE65F comes
from this gene~cDNA EST EM>
emb|CAA90124.2| (Z49910) similarity to 35.1KD hypothetical yeast protein (Swiss
Prot accession number P38805), contains similarity to
Pfam domain: PF01945 (Domain of unknown function),
Score=96.8, E-value=1.3e-25, N=1~cDNA EST CEMSE65F comes
from this gene~cDNA EST EM>
Length = 746
Score = 43.9 bits (102), Expect = 0.001
Identities = 33/140 (23%), Positives = 65/140 (45%), Gaps = 9/140 (6%)
Query: 44 LMIYLKRKNINNMIY--LFENNNRYF-VSIVNFQENIHIKFGINNLLITSRISSIIYK-- 98
L+++ RK N +I+ L E YF ++ + F +++ + + N + + + YK
Sbjct: 574 LVVHEDRKKPNGIIFCHLPEGPTAYFKINSLTFTQDLKVCYFDNFFMYCLKSLKLFYKFG 633
Query: 99 ----DYPEIIYLNFKAQHEIYIQKVLTQILYQVSPLTNRELLCIYSNKGIIYFRSFRYIF 154
+PE+I NF + I ++L + T R ++ ++ + I+FR RY F
Sbjct: 634 ESTSHFPEVILNNFNTRLGHNIARMLACLFPHDPKFTGRRVVTFHNQRDYIFFRHHRYEF 693
Query: 155 SKNLRDVKIQEIGPRLNFKI 174
K + E+GPR ++
Sbjct: 694 KKEGSKAALLELGPRFTLRL 713
>ref|NP_011956.1| Rpf1p [Saccharomyces cerevisiae]
sp|P38805|YHO8_YEAST HYPOTHETICAL 35.1 KDA PROTEIN IN NAM8-GAR1 INTERGENIC REGION
pir||S46718 hypothetical protein YHR088w - yeast (Saccharomyces cerevisiae)
gb|AAB68926.1| (U00060) Yhr088wp [Saccharomyces cerevisiae]
Length = 295
Score = 41.9 bits (97), Expect = 0.006
Identities = 23/74 (31%), Positives = 43/74 (58%), Gaps = 1/74 (1%)
Query: 101 PEIIYLNFKAQHEIYIQKVLTQILYQVSPLTNRELLCIYSNKGIIYFRSFRYIFSKNLRD 160
PE+I NF+ + + ++ IL Q + R+++ +++ + I+FR RY+F N R
Sbjct: 195 PELILNNFQTRLGQTVGRLFQSILPQNPDIEGRQVITLHNQRDYIFFRRHRYVFKDNER- 253
Query: 161 VKIQEIGPRLNFKI 174
V +QE+GP+ K+
Sbjct: 254 VGLQELGPQFTLKL 267
>dbj|BAB14086.1| (AK022537) unnamed protein product [Homo sapiens]
Length = 349
Score = 40.8 bits (94), Expect = 0.012
Identities = 23/74 (31%), Positives = 38/74 (51%), Gaps = 1/74 (1%)
Query: 101 PEIIYLNFKAQHEIYIQKVLTQILYQVSPLTNRELLCIYSNKGIIYFRSFRYIFSKNLRD 160
PEII NF + I ++ + R++ ++ + I+FR RYIF ++ +
Sbjct: 244 PEIILNNFTTRLGHSIGRMFASLFPHNPQFIGRQVATFHNQRDYIFFRFHRYIF-RSEKK 302
Query: 161 VKIQEIGPRLNFKI 174
V IQE+GPR K+
Sbjct: 303 VGIQELGPRFTLKL 316
>emb|CAB55338.1| (AJ006754) hypothetical protein [Yarrowia lipolytica]
Length = 333
Score = 40.0 bits (92), Expect = 0.023
Identities = 20/74 (27%), Positives = 40/74 (54%), Gaps = 1/74 (1%)
Query: 101 PEIIYLNFKAQHEIYIQKVLTQILYQVSPLTNRELLCIYSNKGIIYFRSFRYIFSKNLRD 160
PE+I NF + + ++ + Q R+++ +++ + I+FR RY+F KN
Sbjct: 233 PELILNNFSTRLGKTVGRLFQSMFPQQPEFVGRQVVTLHNQRDWIFFRRHRYVF-KNEER 291
Query: 161 VKIQEIGPRLNFKI 174
V +QE+GP+ ++
Sbjct: 292 VGLQELGPQFTLRL 305
>pir||T50616 hypothetical protein DKFZp761G0415.1 - human (fragment)
Length = 256
Score = 39.6 bits (91), Expect = 0.025
Identities = 23/74 (31%), Positives = 38/74 (51%), Gaps = 1/74 (1%)
Query: 101 PEIIYLNFKAQHEIYIQKVLTQILYQVSPLTNRELLCIYSNKGIIYFRSFRYIFSKNLRD 160
PEII NF + I ++ + R++ ++ + I+FR RYIF ++ +
Sbjct: 151 PEIILNNFTTRLGHSIGRMFASLFPHNPQFIGRQVATFHNQRDYIFFRFHRYIF-RSEKK 209
Query: 161 VKIQEIGPRLNFKI 174
V IQE+GPR K+
Sbjct: 210 VGIQELGPRFTLKL 223
>sp|O14180|YDS4_SCHPO HYPOTHETICAL 35.8 KD PROTEIN C4F8.04 IN CHROMOSOME I
pir||T38834 hypothetical coiled-coil protein - fission yeast
(Schizosaccharomyces pombe)
emb|CAB11051.1| (Z98530) hypothetical coiled-coil protein [Schizosaccharomyces
pombe]
Length = 306
Score = 38.4 bits (88), Expect = 0.065
Identities = 26/114 (22%), Positives = 53/114 (45%), Gaps = 7/114 (6%)
Query: 68 VSIVNFQENIHIKFGINNLLITSRISS--IIYKDYPEIIYLNFKAQHEIYIQKVLTQILY 125
+++V+ F ++NL IS+ PE+I NF + + + + +
Sbjct: 161 LTLVHLPNGPSFYFTLSNLQTAKEISNHGRSTGHIPELIINNFSTRLGMTVARAFQSLFI 220
Query: 126 QVSPLTNRELLCIYSNKGIIYFRSFRYIFSK--NLRD---VKIQEIGPRLNFKI 174
Q + R+++ I+ + ++FR RY F + N+ D +QE+GPR ++
Sbjct: 221 QTPQIQGRQVVTIHCQRDFLFFRRHRYAFREKSNMPDGIGTGLQELGPRFTMRL 274
>gb|AAG38541.1|AF309805_6 (AF309805) coiled-coil protein [Pneumocystis carinii f. sp.
carinii]
Length = 277
Score = 36.9 bits (84), Expect = 0.17
Identities = 33/144 (22%), Positives = 65/144 (44%), Gaps = 7/144 (4%)
Query: 33 YYKRNKFKFRHLMIYLKRKNINNMIYLFENNNRYFVSIVNFQENIHIKFGINNLLITSRI 92
Y K+N R Y K + +F++ + + I++ F I+++ TS I
Sbjct: 106 YQKKN----RQKNTYFFAKEWPGLFPIFQSWLKDSLIIIHLPSGPSFYFTISSITPTSCI 161
Query: 93 --SSIIYKDYPEIIYLNFKAQHEIYIQKVLTQILYQVSPLTNRELLCIYSNKGIIYFRSF 150
PE+I NF + ++ + + + R+++ I++ + I+ R
Sbjct: 162 YRHGRATSHIPELIINNFTTYLGLTVENMFRSLFPTQADFEGRQVVTIHNQRDFIFIRRH 221
Query: 151 RYIFSKNLRDVKIQEIGPRLNFKI 174
RYIF +++ V +QE+GPR K+
Sbjct: 222 RYIFKNDIK-VSLQELGPRFTLKL 244
>emb|CAB77726.1| (AL161492) hypothetical protein [Arabidopsis thaliana]
Length = 343
Score = 35.7 bits (81), Expect = 0.40
Identities = 32/158 (20%), Positives = 67/158 (42%), Gaps = 13/158 (8%)
Query: 33 YYKRNKFKFRHLMIYLKRKNINNMIYLFENNNRY-FVSIVNFQENIHIKFGINNLLITSR 91
Y KR + + ++ Y +K+ ++I + N + I+ F ++NL++
Sbjct: 157 YQKRGTYDLKKIVEYATKKDFTSLIVVHTNRREPDALLIIGLPNGPTAHFKLSNLVLRKD 216
Query: 92 ISS--IIYKDYPEIIYLNFKAQHEIYIQKVLTQILYQVSPLTNRELLCIYSNKGIIYFRS 149
I + PE++ NF + + + + R ++ ++ + I+FR
Sbjct: 217 IKNHGNPTSHQPELVLNNFTTRLGNRVGRFFQSLFPPDPNFRGRRVVTFHNQRDFIFFRH 276
Query: 150 FRYIF----SKNLR------DVKIQEIGPRLNFKILNI 177
RYIF SK+ + ++QE GPR K++ +
Sbjct: 277 HRYIFETKESKSDKGKEETIKPRLQECGPRFTLKLVTL 314
>emb|CAB77655.1| (AJ390518) hypothetical protein [Candida albicans]
Length = 97
Score = 34.5 bits (78), Expect = 0.92
Identities = 19/68 (27%), Positives = 37/68 (53%), Gaps = 1/68 (1%)
Query: 107 NFKAQHEIYIQKVLTQILYQVSPLTNRELLCIYSNKGIIYFRSFRYIFSKNLRDVKIQEI 166
NF ++ + ++ I L R+++ +++ + I+FR RYIF +N V +QE+
Sbjct: 3 NFNSRLGKTVGRLFQSIFPHKPELQGRQVITLHNQRDYIFFRRHRYIF-RNEEKVGLQEL 61
Query: 167 GPRLNFKI 174
GP+ K+
Sbjct: 62 GPQFTLKL 69
>pir||T01938 hypothetical protein F11O4.6 - Arabidopsis thaliana
gb|AAC62782.1| (AF096370) contains similarity to a C. elegans hypothetical protein
F44G4.1 (GB:Z49910) and several yeast hypothetical
proteins such as 35.1 KD protein in NAM8-GAR1 intergenic
region (SP:P38805) [Arabidopsis thaliana]
Length = 434
Score = 32.2 bits (72), Expect = 4.7
Identities = 31/165 (18%), Positives = 66/165 (39%), Gaps = 20/165 (12%)
Query: 33 YYKRNKFKFRHLMIYLKRKNINNMIYLFENNNR--YFVS------IVNFQENIHIKFGIN 84
Y KR + + ++ Y +K+ ++I + N + +S I+ F ++
Sbjct: 170 YQKRGTYDLKKIVEYATKKDFTSLIVVHTNRREPAFAISYVDALLIIGLPNGPTAHFKLS 229
Query: 85 NLLITSRISS--IIYKDYPEIIYLNFKAQHEIYIQKVLTQILYQVSPLTNRELLCIYSNK 142
NL++ I + PE++ NF + + + + R ++ ++ +
Sbjct: 230 NLVLRKDIKNHGNPTSHQPELVLNNFTTRLGNRVGRFFQSLFPPDPNFRGRRVVTFHNQR 289
Query: 143 GIIYFRSFRYIFS----------KNLRDVKIQEIGPRLNFKILNI 177
I+FR RYIF + ++QE GPR K++ +
Sbjct: 290 DFIFFRHHRYIFETKESKSDKGKEETIKPRLQECGPRFTLKLVTL 334
>gb|AAD14602.1| (AF092910) stage specific peptide 24 [Trypanosoma cruzi]
Length = 287
Score = 31.8 bits (71), Expect = 6.3
Identities = 30/139 (21%), Positives = 64/139 (45%), Gaps = 5/139 (3%)
Query: 36 RNKFKFRHLMIYLKRKNINNMIYLFENNN-RYFVSIVNFQENIHIKFGINNLLITSRISS 94
R R LM +R ++++ L E+ +++ + + F I+NL+ I
Sbjct: 116 RGNLSVRQLMDAARRGQYSDVVVLQESQGVPDSLTVSHLPLGPTVVFTIHNLVTRHDIQD 175
Query: 95 I--IYKDYPEIIYLNFKAQHEIYIQKVLTQILYQVSPLTNRELLCIYSNKGIIYFRSFRY 152
+ + + +P +I+ NF + ++ VL + L+ V +L + + FR +
Sbjct: 176 VGTMSEQHPHLIFENFTTRLGRRVRDVL-KFLFPVPKPRPTRVLTFDNQNDFVSFRHHTF 234
Query: 153 IFSKNLRDVKIQEIGPRLN 171
K R+V++ E+GPR++
Sbjct: 235 RAVKG-REVQLTEVGPRMD 252
CPU time: 24.04 user secs. 0.78 sys. secs 24.82 total secs.
Database: nr
Posted date: Feb 10, 2001 7:10 PM
Number of letters in database: 195,544,254
Number of sequences in database: 618,844
Lambda K H
0.330 0.146 0.423
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 57857298
Number of Sequences: 618844
Number of extensions: 2325059
Number of successful extensions: 6877
Number of sequences better than 10.0: 47
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 39
Number of HSP's that attempted gapping in prelim test: 6829
Number of HSP's gapped (non-prelim): 74
length of query: 186
length of database: 195,544,254
effective HSP length: 51
effective length of query: 135
effective length of database: 163,983,210
effective search space: 22137733350
effective search space used: 22137733350
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (21.8 bits)
S2: 69 (31.3 bits)