BLASTP 2.1.1 [Aug-8-2000]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|12580787|emb|CAC27105.1| hypothetical protein
[Guillardia theta]
         (186 letters)

Database: nr
           618,844 sequences; 195,544,254 total letters

Searching..................................................

E-value threshold for inclusion in PSI-Blast iteration 1: 0.002 
E-value threshold for inclusion in PSI-Blast iteration 2:


Distribution of 14 Blast Hits on the Query Sequence




Sequences with E-value BETTER than threshold
Score E Sequences producing significant alignments: (bits) Value emb|CAC27105.1| (AJ010592) hypothetical protein [Guillardia t... 230 7e-60 gb|AAF53162.1| (AE003635) CG6712 gene product [Drosophila mel... 49 5e-05 sp|P54073|YUY1_CAEEL HYPOTHETICAL 87.9 KDA PROTEIN F44G4.1 IN... 44 0.001 pir||T19409 hypothetical protein F44G4.1 - Caenorhabditis ele... 44 0.001
Sequences with E-value WORSE than threshold
ref|NP_011956.1| Rpf1p [Saccharomyces cerevisiae] >gi|731684|... 42 0.006 dbj|BAB14086.1| (AK022537) unnamed protein product [Homo sapi... 41 0.012 emb|CAB55338.1| (AJ006754) hypothetical protein [Yarrowia lip... 40 0.023 pir||T50616 hypothetical protein DKFZp761G0415.1 - human (fra... 40 0.025 sp|O14180|YDS4_SCHPO HYPOTHETICAL 35.8 KD PROTEIN C4F8.04 IN ... 38 0.065 gb|AAG38541.1|AF309805_6 (AF309805) coiled-coil protein [Pneu... 37 0.17 emb|CAB77726.1| (AL161492) hypothetical protein [Arabidopsis ... 36 0.40 emb|CAB77655.1| (AJ390518) hypothetical protein [Candida albi... 35 0.92 pir||T01938 hypothetical protein F11O4.6 - Arabidopsis thalia... 32 4.7 gb|AAD14602.1| (AF092910) stage specific peptide 24 [Trypanos... 32 6.3
Alignments
>emb|CAC27105.1| (AJ010592) hypothetical protein [Guillardia theta]
          Length = 186

 Score =  230 bits (582), Expect = 7e-60
 Identities = 160/186 (86%), Positives = 160/186 (86%)

Query: 1   MKFYXXXXXXXXXXXXXXXXXXXXXXXXXXPRYYKRNKFKFRHLMIYLKRKNINNMIYLF 60
           MKFY                          PRYYKRNKFKFRHLMIYLKRKNINNMIYLF
Sbjct: 1   MKFYSIITTSKKPSKTLLKILHLLKKYLITPRYYKRNKFKFRHLMIYLKRKNINNMIYLF 60

Query: 61  ENNNRYFVSIVNFQENIHIKFGINNLLITSRISSIIYKDYPEIIYLNFKAQHEIYIQKVL 120
           ENNNRYFVSIVNFQENIHIKFGINNLLITSRISSIIYKDYPEIIYLNFKAQHEIYIQKVL
Sbjct: 61  ENNNRYFVSIVNFQENIHIKFGINNLLITSRISSIIYKDYPEIIYLNFKAQHEIYIQKVL 120

Query: 121 TQILYQVSPLTNRELLCIYSNKGIIYFRSFRYIFSKNLRDVKIQEIGPRLNFKILNILSF 180
           TQILYQVSPLTNRELLCIYSNKGIIYFRSFRYIFSKNLRDVKIQEIGPRLNFKILNILSF
Sbjct: 121 TQILYQVSPLTNRELLCIYSNKGIIYFRSFRYIFSKNLRDVKIQEIGPRLNFKILNILSF 180

Query: 181 KINKSI 186
           KINKSI
Sbjct: 181 KINKSI 186
>gb|AAF53162.1| (AE003635) CG6712 gene product [Drosophila melanogaster]
          Length = 394

 Score = 48.9 bits (115), Expect = 5e-05
 Identities = 33/143 (23%), Positives = 70/143 (48%), Gaps = 4/143 (2%)

Query: 36  RNKFKFRHLMIYLKRKNINNMIYLFENNNR-YFVSIVNFQENIHIKFGINNLLITSRIS- 93
           RNK   + +    +R+   +++ + E+  +   + +++        F ++N+ +TS I  
Sbjct: 218 RNKSSVKKICKSAEREEFTDVVIVNEDRRKPNGLLVIHLPNGPTAHFKLSNVKLTSDIKR 277

Query: 94  --SIIYKDYPEIIYLNFKAQHEIYIQKVLTQILYQVSPLTNRELLCIYSNKGIIYFRSFR 151
               I K  PE+I  NF  +  + + ++L  + +       R  +  ++ +  I+FR  R
Sbjct: 278 DHKEITKHRPEVILNNFTTRLGLTVGRMLGALFHHDPEFRGRRAVTFHNQRDYIFFRHHR 337

Query: 152 YIFSKNLRDVKIQEIGPRLNFKI 174
           Y F+K  + VK++E+GPR   K+
Sbjct: 338 YEFTKEGKRVKLRELGPRFTLKL 360
>sp|P54073|YUY1_CAEEL HYPOTHETICAL 87.9 KDA PROTEIN F44G4.1 IN CHROMOSOME II PRECURSOR
          Length = 754

 Score = 43.9 bits (102), Expect = 0.001
 Identities = 33/140 (23%), Positives = 65/140 (45%), Gaps = 9/140 (6%)

Query: 44  LMIYLKRKNINNMIY--LFENNNRYF-VSIVNFQENIHIKFGINNLLITSRISSIIYK-- 98
           L+++  RK  N +I+  L E    YF ++ + F +++ + +  N  +   +   + YK  
Sbjct: 582 LVVHEDRKKPNGIIFCHLPEGPTAYFKINSLTFTQDLKVCYFDNFFMYCLKSLKLFYKFG 641

Query: 99  ----DYPEIIYLNFKAQHEIYIQKVLTQILYQVSPLTNRELLCIYSNKGIIYFRSFRYIF 154
                +PE+I  NF  +    I ++L  +       T R ++  ++ +  I+FR  RY F
Sbjct: 642 ESTSHFPEVILNNFNTRLGHNIARMLACLFPHDPKFTGRRVVTFHNQRDYIFFRHHRYEF 701

Query: 155 SKNLRDVKIQEIGPRLNFKI 174
            K      + E+GPR   ++
Sbjct: 702 KKEGSKAALLELGPRFTLRL 721
>pir||T19409 hypothetical protein F44G4.1 - Caenorhabditis elegans
 emb|CAA93858.2| (Z70034) similarity to 35.1KD hypothetical yeast protein (Swiss
           Prot accession number P38805), contains similarity to
           Pfam domain: PF01945 (Domain of unknown function),
           Score=96.8, E-value=1.3e-25, N=1~cDNA EST CEMSE65F comes
           from this gene~cDNA EST EM>
 emb|CAA90124.2| (Z49910) similarity to 35.1KD hypothetical yeast protein (Swiss
           Prot accession number P38805), contains similarity to
           Pfam domain: PF01945 (Domain of unknown function),
           Score=96.8, E-value=1.3e-25, N=1~cDNA EST CEMSE65F comes
           from this gene~cDNA EST EM>
          Length = 746

 Score = 43.9 bits (102), Expect = 0.001
 Identities = 33/140 (23%), Positives = 65/140 (45%), Gaps = 9/140 (6%)

Query: 44  LMIYLKRKNINNMIY--LFENNNRYF-VSIVNFQENIHIKFGINNLLITSRISSIIYK-- 98
           L+++  RK  N +I+  L E    YF ++ + F +++ + +  N  +   +   + YK  
Sbjct: 574 LVVHEDRKKPNGIIFCHLPEGPTAYFKINSLTFTQDLKVCYFDNFFMYCLKSLKLFYKFG 633

Query: 99  ----DYPEIIYLNFKAQHEIYIQKVLTQILYQVSPLTNRELLCIYSNKGIIYFRSFRYIF 154
                +PE+I  NF  +    I ++L  +       T R ++  ++ +  I+FR  RY F
Sbjct: 634 ESTSHFPEVILNNFNTRLGHNIARMLACLFPHDPKFTGRRVVTFHNQRDYIFFRHHRYEF 693

Query: 155 SKNLRDVKIQEIGPRLNFKI 174
            K      + E+GPR   ++
Sbjct: 694 KKEGSKAALLELGPRFTLRL 713
>ref|NP_011956.1| Rpf1p [Saccharomyces cerevisiae]
 sp|P38805|YHO8_YEAST HYPOTHETICAL 35.1 KDA PROTEIN IN NAM8-GAR1 INTERGENIC REGION
 pir||S46718 hypothetical protein YHR088w - yeast (Saccharomyces cerevisiae)
 gb|AAB68926.1| (U00060) Yhr088wp [Saccharomyces cerevisiae]
          Length = 295

 Score = 41.9 bits (97), Expect = 0.006
 Identities = 23/74 (31%), Positives = 43/74 (58%), Gaps = 1/74 (1%)

Query: 101 PEIIYLNFKAQHEIYIQKVLTQILYQVSPLTNRELLCIYSNKGIIYFRSFRYIFSKNLRD 160
           PE+I  NF+ +    + ++   IL Q   +  R+++ +++ +  I+FR  RY+F  N R 
Sbjct: 195 PELILNNFQTRLGQTVGRLFQSILPQNPDIEGRQVITLHNQRDYIFFRRHRYVFKDNER- 253

Query: 161 VKIQEIGPRLNFKI 174
           V +QE+GP+   K+
Sbjct: 254 VGLQELGPQFTLKL 267
>dbj|BAB14086.1| (AK022537) unnamed protein product [Homo sapiens]
          Length = 349

 Score = 40.8 bits (94), Expect = 0.012
 Identities = 23/74 (31%), Positives = 38/74 (51%), Gaps = 1/74 (1%)

Query: 101 PEIIYLNFKAQHEIYIQKVLTQILYQVSPLTNRELLCIYSNKGIIYFRSFRYIFSKNLRD 160
           PEII  NF  +    I ++   +         R++   ++ +  I+FR  RYIF ++ + 
Sbjct: 244 PEIILNNFTTRLGHSIGRMFASLFPHNPQFIGRQVATFHNQRDYIFFRFHRYIF-RSEKK 302

Query: 161 VKIQEIGPRLNFKI 174
           V IQE+GPR   K+
Sbjct: 303 VGIQELGPRFTLKL 316
>emb|CAB55338.1| (AJ006754) hypothetical protein [Yarrowia lipolytica]
          Length = 333

 Score = 40.0 bits (92), Expect = 0.023
 Identities = 20/74 (27%), Positives = 40/74 (54%), Gaps = 1/74 (1%)

Query: 101 PEIIYLNFKAQHEIYIQKVLTQILYQVSPLTNRELLCIYSNKGIIYFRSFRYIFSKNLRD 160
           PE+I  NF  +    + ++   +  Q      R+++ +++ +  I+FR  RY+F KN   
Sbjct: 233 PELILNNFSTRLGKTVGRLFQSMFPQQPEFVGRQVVTLHNQRDWIFFRRHRYVF-KNEER 291

Query: 161 VKIQEIGPRLNFKI 174
           V +QE+GP+   ++
Sbjct: 292 VGLQELGPQFTLRL 305
>pir||T50616 hypothetical protein DKFZp761G0415.1 - human (fragment)
          Length = 256

 Score = 39.6 bits (91), Expect = 0.025
 Identities = 23/74 (31%), Positives = 38/74 (51%), Gaps = 1/74 (1%)

Query: 101 PEIIYLNFKAQHEIYIQKVLTQILYQVSPLTNRELLCIYSNKGIIYFRSFRYIFSKNLRD 160
           PEII  NF  +    I ++   +         R++   ++ +  I+FR  RYIF ++ + 
Sbjct: 151 PEIILNNFTTRLGHSIGRMFASLFPHNPQFIGRQVATFHNQRDYIFFRFHRYIF-RSEKK 209

Query: 161 VKIQEIGPRLNFKI 174
           V IQE+GPR   K+
Sbjct: 210 VGIQELGPRFTLKL 223
>sp|O14180|YDS4_SCHPO HYPOTHETICAL 35.8 KD PROTEIN C4F8.04 IN CHROMOSOME I
 pir||T38834 hypothetical coiled-coil protein - fission yeast
           (Schizosaccharomyces pombe)
 emb|CAB11051.1| (Z98530) hypothetical coiled-coil protein [Schizosaccharomyces
           pombe]
          Length = 306

 Score = 38.4 bits (88), Expect = 0.065
 Identities = 26/114 (22%), Positives = 53/114 (45%), Gaps = 7/114 (6%)

Query: 68  VSIVNFQENIHIKFGINNLLITSRISS--IIYKDYPEIIYLNFKAQHEIYIQKVLTQILY 125
           +++V+        F ++NL     IS+        PE+I  NF  +  + + +    +  
Sbjct: 161 LTLVHLPNGPSFYFTLSNLQTAKEISNHGRSTGHIPELIINNFSTRLGMTVARAFQSLFI 220

Query: 126 QVSPLTNRELLCIYSNKGIIYFRSFRYIFSK--NLRD---VKIQEIGPRLNFKI 174
           Q   +  R+++ I+  +  ++FR  RY F +  N+ D     +QE+GPR   ++
Sbjct: 221 QTPQIQGRQVVTIHCQRDFLFFRRHRYAFREKSNMPDGIGTGLQELGPRFTMRL 274
>gb|AAG38541.1|AF309805_6 (AF309805) coiled-coil protein [Pneumocystis carinii f. sp.
           carinii]
          Length = 277

 Score = 36.9 bits (84), Expect = 0.17
 Identities = 33/144 (22%), Positives = 65/144 (44%), Gaps = 7/144 (4%)

Query: 33  YYKRNKFKFRHLMIYLKRKNINNMIYLFENNNRYFVSIVNFQENIHIKFGINNLLITSRI 92
           Y K+N    R    Y   K    +  +F++  +  + I++        F I+++  TS I
Sbjct: 106 YQKKN----RQKNTYFFAKEWPGLFPIFQSWLKDSLIIIHLPSGPSFYFTISSITPTSCI 161

Query: 93  --SSIIYKDYPEIIYLNFKAQHEIYIQKVLTQILYQVSPLTNRELLCIYSNKGIIYFRSF 150
                     PE+I  NF     + ++ +   +    +    R+++ I++ +  I+ R  
Sbjct: 162 YRHGRATSHIPELIINNFTTYLGLTVENMFRSLFPTQADFEGRQVVTIHNQRDFIFIRRH 221

Query: 151 RYIFSKNLRDVKIQEIGPRLNFKI 174
           RYIF  +++ V +QE+GPR   K+
Sbjct: 222 RYIFKNDIK-VSLQELGPRFTLKL 244
>emb|CAB77726.1| (AL161492) hypothetical protein [Arabidopsis thaliana]
          Length = 343

 Score = 35.7 bits (81), Expect = 0.40
 Identities = 32/158 (20%), Positives = 67/158 (42%), Gaps = 13/158 (8%)

Query: 33  YYKRNKFKFRHLMIYLKRKNINNMIYLFENNNRY-FVSIVNFQENIHIKFGINNLLITSR 91
           Y KR  +  + ++ Y  +K+  ++I +  N      + I+         F ++NL++   
Sbjct: 157 YQKRGTYDLKKIVEYATKKDFTSLIVVHTNRREPDALLIIGLPNGPTAHFKLSNLVLRKD 216

Query: 92  ISS--IIYKDYPEIIYLNFKAQHEIYIQKVLTQILYQVSPLTNRELLCIYSNKGIIYFRS 149
           I +        PE++  NF  +    + +    +         R ++  ++ +  I+FR 
Sbjct: 217 IKNHGNPTSHQPELVLNNFTTRLGNRVGRFFQSLFPPDPNFRGRRVVTFHNQRDFIFFRH 276

Query: 150 FRYIF----SKNLR------DVKIQEIGPRLNFKILNI 177
            RYIF    SK+ +        ++QE GPR   K++ +
Sbjct: 277 HRYIFETKESKSDKGKEETIKPRLQECGPRFTLKLVTL 314
>emb|CAB77655.1| (AJ390518) hypothetical protein [Candida albicans]
          Length = 97

 Score = 34.5 bits (78), Expect = 0.92
 Identities = 19/68 (27%), Positives = 37/68 (53%), Gaps = 1/68 (1%)

Query: 107 NFKAQHEIYIQKVLTQILYQVSPLTNRELLCIYSNKGIIYFRSFRYIFSKNLRDVKIQEI 166
           NF ++    + ++   I      L  R+++ +++ +  I+FR  RYIF +N   V +QE+
Sbjct: 3   NFNSRLGKTVGRLFQSIFPHKPELQGRQVITLHNQRDYIFFRRHRYIF-RNEEKVGLQEL 61

Query: 167 GPRLNFKI 174
           GP+   K+
Sbjct: 62  GPQFTLKL 69
>pir||T01938 hypothetical protein F11O4.6 - Arabidopsis thaliana
 gb|AAC62782.1| (AF096370) contains similarity to a C. elegans hypothetical protein
           F44G4.1 (GB:Z49910) and several yeast hypothetical
           proteins such as 35.1 KD protein in NAM8-GAR1 intergenic
           region (SP:P38805) [Arabidopsis thaliana]
          Length = 434

 Score = 32.2 bits (72), Expect = 4.7
 Identities = 31/165 (18%), Positives = 66/165 (39%), Gaps = 20/165 (12%)

Query: 33  YYKRNKFKFRHLMIYLKRKNINNMIYLFENNNR--YFVS------IVNFQENIHIKFGIN 84
           Y KR  +  + ++ Y  +K+  ++I +  N     + +S      I+         F ++
Sbjct: 170 YQKRGTYDLKKIVEYATKKDFTSLIVVHTNRREPAFAISYVDALLIIGLPNGPTAHFKLS 229

Query: 85  NLLITSRISS--IIYKDYPEIIYLNFKAQHEIYIQKVLTQILYQVSPLTNRELLCIYSNK 142
           NL++   I +        PE++  NF  +    + +    +         R ++  ++ +
Sbjct: 230 NLVLRKDIKNHGNPTSHQPELVLNNFTTRLGNRVGRFFQSLFPPDPNFRGRRVVTFHNQR 289

Query: 143 GIIYFRSFRYIFS----------KNLRDVKIQEIGPRLNFKILNI 177
             I+FR  RYIF           +     ++QE GPR   K++ +
Sbjct: 290 DFIFFRHHRYIFETKESKSDKGKEETIKPRLQECGPRFTLKLVTL 334
>gb|AAD14602.1| (AF092910) stage specific peptide 24 [Trypanosoma cruzi]
          Length = 287

 Score = 31.8 bits (71), Expect = 6.3
 Identities = 30/139 (21%), Positives = 64/139 (45%), Gaps = 5/139 (3%)

Query: 36  RNKFKFRHLMIYLKRKNINNMIYLFENNN-RYFVSIVNFQENIHIKFGINNLLITSRISS 94
           R     R LM   +R   ++++ L E+      +++ +      + F I+NL+    I  
Sbjct: 116 RGNLSVRQLMDAARRGQYSDVVVLQESQGVPDSLTVSHLPLGPTVVFTIHNLVTRHDIQD 175

Query: 95  I--IYKDYPEIIYLNFKAQHEIYIQKVLTQILYQVSPLTNRELLCIYSNKGIIYFRSFRY 152
           +  + + +P +I+ NF  +    ++ VL + L+ V       +L   +    + FR   +
Sbjct: 176 VGTMSEQHPHLIFENFTTRLGRRVRDVL-KFLFPVPKPRPTRVLTFDNQNDFVSFRHHTF 234

Query: 153 IFSKNLRDVKIQEIGPRLN 171
              K  R+V++ E+GPR++
Sbjct: 235 RAVKG-REVQLTEVGPRMD 252
CPU time:    24.04 user secs.	    0.78 sys. secs	   24.82 total secs.

  Database: nr
    Posted date:  Feb 10, 2001  7:10 PM
  Number of letters in database: 195,544,254
  Number of sequences in database:  618,844
  
Lambda     K      H
   0.330    0.146    0.423 

Gapped
Lambda     K      H
   0.270   0.0470    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 57857298
Number of Sequences: 618844
Number of extensions: 2325059
Number of successful extensions: 6877
Number of sequences better than 10.0: 47
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 39
Number of HSP's that attempted gapping in prelim test: 6829
Number of HSP's gapped (non-prelim): 74
length of query: 186
length of database: 195,544,254
effective HSP length: 51
effective length of query: 135
effective length of database: 163,983,210
effective search space: 22137733350
effective search space used: 22137733350
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (21.8 bits)
S2: 69 (31.3 bits)