ID NEF_HV2D1 STANDARD; PRT; 257 AA. AC P17753; DT 01-AUG-1990 (Rel. 15, Created) DT 01-AUG-1990 (Rel. 15, Last sequence update) DT 01-JUL-1993 (Rel. 26, Last annotation update) DE NEGATIVE FACTOR (F-PROTEIN) (27 KDA PROTEIN) (3'ORF). GN NEF. OS Human immunodeficiency virus type 2 (isolate D194) (HIV-2). OC Viruses; Retroid viruses; Retroviridae; Lentivirus. OX NCBI_TaxID=11713; RN [1] RP SEQUENCE FROM N.A. RX MEDLINE=89184631; PubMed=2467304; RA Kuehnel H., von Briesen H., Dietrich U., Adamski M., Mix D., RA Biesert L., Kreutz R., Immelmann A., Henco K., Meichsner C., RA Andreesen R., Gelderblom H., Ruebsamen-Waigmann H.; RT "Molecular cloning of two west African human immunodeficiency virus RT type 2 isolates that replicate well in macrophages: a Gambian isolate, RT from a patient with neurologic acquired immunodeficiency syndrome, and RT a highly divergent Ghanian isolate."; RL Proc. Natl. Acad. Sci. U.S.A. 86:2383-2387(1989). RN [2] RP SEQUENCE FROM N.A. RX MEDLINE=91045094; PubMed=2235509; RA Kuehnel H., Kreutz R., Ruebsamen-Waigmann H.; RT "Nucleotide sequence of HIV-2D194, an isolate from a Gambian case of RT 'neuro-AIDS', which showed excellent growth in macrophages."; RL Nucleic Acids Res. 18:6142-6142(1990). CC -!- FUNCTION: NEF HAS GTPASE, GTP-BINDING AND AUTOPHOSPHORYLATING CC ACTIVITIES, IT SEEM TO DOWN REGULATE THE CD4(T4) ANTIGEN. CC -!- MISCELLANEOUS: THIS ISOLATE IS FROM A GAMBIAN CASE OF CC 'NEURO-AIDS'. CC -------------------------------------------------------------------------- CC This SWISS-PROT entry is copyright. It is produced through a collaboration CC between the Swiss Institute of Bioinformatics and the EMBL outstation - CC the European Bioinformatics Institute. There are no restrictions on its CC use by non-profit institutions as long as its content is in no way CC modified and this statement is not removed. Usage by and for commercial CC entities requires a license agreement (See http://www.isb-sib.ch/announce/ CC or send an email to license@isb-sib.ch). CC -------------------------------------------------------------------------- DR EMBL; J04542; AAA76848.1; -. DR EMBL; X52223; CAA36472.1; -. DR PIR; S12160; S12160. DR HSSP; P03406; 1EFN. DR HIV; J04542; NEF$2D194. DR INTERPRO; IPR001558; -. DR PFAM; PF00469; F-protein; 1. KW AIDS; Myristate; GTP-binding. FT LIPID 2 2 MYRISTATE (BY SIMILARITY). SQ SEQUENCE 257 AA; 29449 MW; 2C5E1EB5C8ABBCF3 CRC64; MGASGSKKRS EHSQGLRERL LRARGGGYVK QRNASGGESS QSQEGSGREQ KSPSCEGQQY QQGEFMNTPW RTPAAIGQKN SYKQQNMDDV DSDDDDLVGV PVMPRVPLRE MTYKLAIDMS HFIKEKGGLE GIFYSRERHR ILDLFLEKEE GIIPDWQNYT HGPGTRYPMY FGWLWKLVPV DISQEAEEVE TNCLVHPAQT SRYDDEHGET LVWRFDPMLA YSYKAFILHP EEFGHKSGLP EKEWKAKLKA RGIPYSE //