ID   PONA_DICDI     STANDARD;      PRT;   143 AA.
AC   P54660;
DT   01-OCT-1996 (REL. 34, CREATED)
DT   01-OCT-1996 (REL. 34, LAST SEQUENCE UPDATE)
DT   01-NOV-1997 (REL. 35, LAST ANNOTATION UPDATE)
DE   PONTICULIN PRECURSOR.
GN   PONA.
OS   DICTYOSTELIUM DISCOIDEUM (SLIME MOLD).
OC   EUKARYOTA; PROTOZOA; SARCOMASTIGOPHORA; SARCODINA; RHIZOPODA;
OC   EUMYCETOZOA; DICTYOSTELIA.
RN   [1]
RP   SEQUENCE FROM N.A., AND PARTIAL SEQUENCE.
RC   STRAIN=AX3;
RX   MEDLINE; 94375519.
RA   HITT A.L., LU T.H., LUNA E.J.;
RL   J. CELL BIOL. 126:1421-1431(1994).
RN   [2]
RP   SEQUENCE OF 23-45.
RX   MEDLINE; 91238542.
RA   WUESTEHUBE L.J., SPEICHER D.W., SHARIFF A., LUNA E.J.;
RL   METH. ENZYMOL. 196:47-65(1991).
RN   [3]
RP   CHARACTERIZATION.
RX   MEDLINE; 94375520.
RA   HITT A.L., HARTWIG J.H., LUNA E.J.;
RL   J. CELL BIOL. 126:1433-1444(1994).
CC   -!- FUNCTION: BINDS F-ACTIN AND NUCLEATES ACTIN ASSEMBLY. MAJOR HIGH
CC       AFFINITY LINK BETWEEN THE PLASMA MEMBRANE AND THE CORTICAL ACTIN
CC       NETWORK.
CC   -!- SUBUNIT: MONOMER.
CC   -!- SUBCELLULAR LOCATION: INTEGRAL MEMBRANE PROTEIN ALSO ATTACHED TO
CC       THE PLASMA MEMBRANE BY A GPI-ANCHOR. PREFERENTIALLY LOCALIZED IN
CC       SOME ACTIN-RICH MEMBRANE STRUCTURES. MUST SPAN THE PLASMA
CC       MEMBRANE, PROBABLY VIA BETA-STRANDS.
CC   -!- DEVELOPMENTAL STAGE: HIGH EXPRESSION DURING GROWTH AND EARLY
CC       DEVELOPMENT WHICH DROPS PRECIPITOUSLY DURING CELL STREAMING AT
CC       CIRCA 8 HOURS OF DEVELOPMENT.
CC   -!- PTM: DISULFIDE BOND(S) STABILIZE THE NATIVE, ACTIN-BINDING
CC       CONFORMATION OF PONTICULIN.
DR   EMBL; Z36528; G530016; -.
DR   EMBL; Z36534; G531358; -.
DR   EMBL; Z36535; G531360; -.
DR   DICTYDB; DD01106; PONA.
KW   GLYCOPROTEIN; MEMBRANE; GPI-ANCHOR; ACTIN-BINDING; SIGNAL;
KW   TRANSMEMBRANE.
FT   SIGNAL        1     22
FT   CHAIN        23    118       PONTICULIN.
FT   PROPEP      119    143       REMOVED IN MATURE FORM (POTENTIAL).
FT   CARBOHYD    111    111       POTENTIAL.
FT   LIPID       118    118       GPI-ANCHOR (POTENTIAL).
SQ   SEQUENCE   143 AA;  14770 MW;  FDAD6A41 CRC32;
     MLVLRNLLAL VTLALLFTLS SAQYTLSVSN SASGSKCTTA VSAKLNACNT GCLNSFNIVE
     SSNGKGLVFK TFINAACSGE YESLSQFTCA ANQKIPTTSY IVSCNSTPSS NSTTDSDSSS
     GSTVMIGLAS SLLFAFATLL ALF
//