ID HYA1_HUMAN STANDARD; PRT; 509 AA. AC P38567; DT 01-OCT-1994 (REL. 30, CREATED) DT 01-OCT-1994 (REL. 30, LAST SEQUENCE UPDATE) DT 15-JUL-1998 (REL. 36, LAST ANNOTATION UPDATE) DE HYALURONIDASE PRECURSOR (EC 3.2.1.35) (SPERM SURFACE PROTEIN PH-20) DE (SPERM ADHESION MOLECULE 1). GN SPAM1 OR HYAL1 OR PH20. OS HOMO SAPIENS (HUMAN). OC EUKARYOTA; METAZOA; CHORDATA; VERTEBRATA; TETRAPODA; MAMMALIA; OC EUTHERIA; PRIMATES. RN [1] RP SEQUENCE FROM N.A. RC TISSUE=TESTIS; RX MEDLINE; 94052105. RA LIN Y., KIMMEL L.H., MYLES D.G., PRIMAKOFF P.; RL PROC. NATL. ACAD. SCI. U.S.A. 90:10071-10075(1993). RN [2] RP SEQUENCE FROM N.A. RC TISSUE=TESTIS; RX MEDLINE; 94109604. RA GMACHL M., SAGAN S., KETTER S., KREIL G.; RL FEBS LETT. 336:545-548(1993). RN [3] RP SEQUENCE FROM N.A. RC TISSUE=TESTIS; RX MEDLINE; 96121399. RA JONES M.H., DAVEY P.M., APLIN H., AFFARA N.A.; RL GENOMICS 29:796-800(1995). CC -!- FUNCTION: INVOLVED IN SPERM-EGG ADHESION. UPON FERTILIZATION CC SPERM MUST FIRST PENETRATE A LAYER OF CUMULUS CELLS THAT CC SURROUNDS THE EGG BEFORE REACHING THE ZONA PELLUCIDA. THE CC CUMULUS CELLS ARE EMBEDDED IN A MATRIX CONTAINING HYALURONIC CC ACID WHICH IS FORMED PRIOR TO OVULATION. THIS PROTEIN AIDS IN CC PENETRATING THE LAYER OF CUMULUS CELLS BY DIGESTING HYALURONIC CC ACID. CC -!- CATALYTIC ACTIVITY: RANDOM HYDROLYSIS OF 1,4-LINKAGES BETWEEN CC N-ACETYL-BETA-D-GLUCOSAMINE AND D-GLUCURONATE RESIDUES IN CC HYALURONATE. CC -!- SUBCELLULAR LOCATION: ATTACHED TO THE MEMBRANE BY A GPI-ANCHOR. CC -!- TISSUE SPECIFICITY: TESTIS. CC -!- SIMILARITY: BELONGS TO FAMILY 56 OF GLYCOSYL HYDROLASES. DR EMBL; L13781; -; NOT_ANNOTATED_CDS. DR EMBL; S67798; G459387; -. DR EMBL; X84347; E256805; -. DR PIR; A48795; A48795. DR MIM; 600930; -. KW HYDROLASE; GLYCOSIDASE; SPERM; GLYCOPROTEIN; SIGNAL; GPI-ANCHOR. FT SIGNAL 1 35 BY SIMILARITY. FT CHAIN 36 490 HYALURONIDASE. FT PROPEP 491 509 REMOVED IN MATURE FORM (POTENTIAL). FT CARBOHYD 82 82 POTENTIAL. FT CARBOHYD 166 166 POTENTIAL. FT CARBOHYD 235 235 POTENTIAL. FT CARBOHYD 254 254 POTENTIAL. FT CARBOHYD 368 368 POTENTIAL. FT CARBOHYD 393 393 POTENTIAL. FT LIPID 490 490 GPI-ANCHOR (POTENTIAL). FT CONFLICT 48 48 P -> A (IN REF. 2). FT CONFLICT 499 499 L -> W (IN REF. 2). SQ SEQUENCE 509 AA; 57847 MW; 8807D1C5 CRC32; MGVLKFKHIF FRSFVKSSGV SQIVFTFLLI PCCLTLNFRA PPVIPNVPFL WAWNAPSEFC LGKFDEPLDM SLFSFIGSPR INATGQGVTI FYVDRLGYYP YIDSITGVTV NGGIPQKISL QDHLDKAKKD ITFYMPVDNL GMAVIDWEEW RPTWARNWKP KDVYKNRSIE LVQQQNVQLS LTEATEKAKQ EFEKAGKDFL VETIKLGKLL RPNHLWGYYL FPDCYNHHYK KPGYNGSCFN VEIKRNDDLS WLWNESTALY PSIYLNTQQS PVAATLYVRN RVREAIRVSK IPDAKSPLPV FAYTRIVFTD QVLKFLSQDE LVYTFGETVA LGASGIVIWG TLSIMRSMKS CLLLDNYMET ILNPYIINVT LAAKMCSQVL CQEQGVCIRK NWNSSDYLHL NPDNFAIQLE KGGKFTVRGK PTLEDLEQFS EKFYCSCYST LSCKEKADVK DTDAVDVCIA DGVCIDAFLK PPMETEEPQI FYNASPSTLS ATMFIVSILF LIISSVASL //