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GPI Lipid Anchor Project Sequence analysis of GPI-anchored proteins on the proprotein level Prediction of potential GPI-modification sites in proprotein sequences Big-PI predictor Occurence of potentially GPI modified proteins in complete genomes Prediction of GPI lipid anchors in plants Big-PI Plant Predictor Prediction of GPI lipid anchors in fungi Big-PI Fungal Predictor

Occurence of potentially GPI modified proteins in fungi

Contact:
Birgit Eisenhaber (IMP/Austria)
Georg Schneider (IMP/Austria)
Michael Wildpaner (IMP/Austria)
Frank Eisenhaber (IMP/Austria)

Link to the big-PI fungal predictor

Data sheets: Learning set and prediction results

1. Derivation of motif properties

Comparison with amino acid property scales
Analysis of volume compensation in the omega-site region

2. Details of prediction method derivation and validation

Prediction function parametrization
The fungal learning set - a list of 254 proteins
The self-consistency-test of the fungal learning set
The jack-knife-test I over the whole fungal learning set
The jack-knife-test II over the largest subset of non-homologous sequences only
The prediction of the GAS1 mutation set (Nuoffer C. et al, J Biol Chem. 1993 May 15;268(14):10558-63.)

3. Prediction results: Occurence of potentially GPI modified proteins in

the Aspergillus nidulans genome (August 2003)
the Candida albicans genome (June 2003)
the Neurospora crassa genome (June 2003)
the Saccharomyces cerevisiae genome (June 2003)
the Schizosaccharomyces pombe genome (June 2003)
the SPTrEMBL database (June 2003, fun.dat)
the SWISSPROT database (rel. 41, Fungi)

4. Functional classification of

Aspergillus nidulans hits
Candida albicans hits
Neurospora crassa hits
Saccharomyces cerevisiae hits
Schizosaccharomyces pombe hits

5. Comparison with previous published data

comparison with S.cerevisiae protein list created by Caro L.H. et al. (1997)
comparison with S.cerevisiae protein list created by Hamada K. et al. (1998)
comparison with C.albicans, N.crassa, S.cerevisiae, and S.pombe protein lists created by de Groot et al. (2003)

Acknowledgement:

Aspergillus nidulans:
http://www-genome.wi.mit.edu/cgi-bin/annotation/aspergillus/
Candida albicans:
http://www-sequence.stanford.edu/group/candida/download.html
Neurospora crassa:
http://www-genome.wi.mit.edu/ftp/pub/annotation/neurospora/assembly3/
Saccharomyces cerevisiae:
ftp://genome-ftp.stanford.edu/pub/yeast/data_download/sequence/genomic_sequence/orf_protein/
Schizosaccharomyces pombe :
ftp://ftp.sanger.ac.uk/pub/yeast/pombe/Protein_data/pompep

Last modified: 17th September 2003