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GPI Anchor Biosynthesis Report: T24913 (PIG-S family, Caenorhabditis elegans)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query=
         (544 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!


Results of PSI-Blast iteration 3

Distribution of 13 Blast Hits on the Query Sequence


Sequences with E-value BETTER than threshold (0.002)

ref|NP_501852.1| (NM_069451) T14G10.7.p [Caenorhabditis elegans]... 773 0.0
ref|NP_149975.1| (NM_033198) phosphatidylinositol glycan, class ... 585 e-166
gb|AAL27645.1| (AY061834) SD10476p [Drosophila melanogaster] 564 e-159
ref|NP_010722.1| (NC_001136) Glycosyl Phosphatidyl Inositol 17; ... 509 e-143
sp|Q10351|YDA9_SCHPO HYPOTHETICAL 63.9 KD PROTEIN C1F12.09 IN CH... 489 e-137
gb|AAL32707.1| (AY062629) Unknown protein [Arabidopsis thaliana] 367 e-100
gb|AAF56365.1| (AE003749) CG13644 gene product [Drosophila melan... 356 6e-97
ref|NP_187374.1| (NM_111598) unknown protein [Arabidopsis thalia... 338 2e-91
ref|XP_091596.1| (XM_091596) similar to phosphatidylinositol gly... 230 5e-59
gb|AAF56366.1| (AE003749) CG6031 gene product [Drosophila melano... 186 7e-46
dbj|BAB70938.1| (AK055512) unnamed protein product [Homo sapiens] 160 4e-38
Sequences with E-value WORSE than threshold

ref|NP_540827.1| (NC_003317) RECOMBINATION PROTEIN RECR [Brucell... 33 9.4
Alignments
>ref|NP_501852.1| (NM_069451) T14G10.7.p [Caenorhabditis elegans]
 pir||T24913 hypothetical protein T14G10.7 - Caenorhabditis elegans
 emb|CAA93093.1| (Z68880) predicted using Genefinder~cDNA EST EMBL:M89453 comes from
           this gene~cDNA EST yk111h3.3 comes from this gene~cDNA
           EST yk111h3.5 comes from this gene~cDNA EST yk457b10.3
           comes from this gene~cDNA EST yk457b10.5 comes from this
           gene~cDNA EST yk3>
          Length = 544

 Score =  773 bits (1976), Expect = 0.0
 Identities = 544/544 (100%), Positives = 544/544 (100%)

Query: 1   MPQESSKSFKQKALDFLLGRQTETEEYGEITQSFINYVHSERPYKLAASLSFVILVFFIG 60
           MPQESSKSFKQKALDFLLGRQTETEEYGEITQSFINYVHSERPYKLAASLSFVILVFFIG
Sbjct: 1   MPQESSKSFKQKALDFLLGRQTETEEYGEITQSFINYVHSERPYKLAASLSFVILVFFIG 60

Query: 61  MPMWHFTTSTYRSNFPTFPSNQSITVPIRVIFVITSEDLRNDVKTLMNELEVNMTKKEVV 120
           MPMWHFTTSTYRSNFPTFPSNQSITVPIRVIFVITSEDLRNDVKTLMNELEVNMTKKEVV
Sbjct: 61  MPMWHFTTSTYRSNFPTFPSNQSITVPIRVIFVITSEDLRNDVKTLMNELEVNMTKKEVV 120

Query: 121 APLDFTWTTQFIGLRSFEHLEQDHSIQIEENKEFFDVFVALVPEKEWTHYSATRIQLGIG 180
           APLDFTWTTQFIGLRSFEHLEQDHSIQIEENKEFFDVFVALVPEKEWTHYSATRIQLGIG
Sbjct: 121 APLDFTWTTQFIGLRSFEHLEQDHSIQIEENKEFFDVFVALVPEKEWTHYSATRIQLGIG 180

Query: 181 KWNFIQWPTKSEEKQKASTRVTDLVSETLIDVPHLNSIVRRDLRQKMQPWQIAALPLSHQ 240
           KWNFIQWPTKSEEKQKASTRVTDLVSETLIDVPHLNSIVRRDLRQKMQPWQIAALPLSHQ
Sbjct: 181 KWNFIQWPTKSEEKQKASTRVTDLVSETLIDVPHLNSIVRRDLRQKMQPWQIAALPLSHQ 240

Query: 241 KRLVWDSAPLATNYHIQVIHLHENSEITPESLKEQEKTARALRKFAEKVKEITNVEVSFE 300
           KRLVWDSAPLATNYHIQVIHLHENSEITPESLKEQEKTARALRKFAEKVKEITNVEVSFE
Sbjct: 241 KRLVWDSAPLATNYHIQVIHLHENSEITPESLKEQEKTARALRKFAEKVKEITNVEVSFE 300

Query: 301 HLWDFEPTRGFLIKDVQERWALTHSAMEELVKKVDSQLQTSLQHGAVLRFVVLETVEPVI 360
           HLWDFEPTRGFLIKDVQERWALTHSAMEELVKKVDSQLQTSLQHGAVLRFVVLETVEPVI
Sbjct: 301 HLWDFEPTRGFLIKDVQERWALTHSAMEELVKKVDSQLQTSLQHGAVLRFVVLETVEPVI 360

Query: 361 VLDDTGEDSHGVAVASWGAVLPRNEATESRAIAAIRIQMGMDAELSHGWHRPPVAICQWE 420
           VLDDTGEDSHGVAVASWGAVLPRNEATESRAIAAIRIQMGMDAELSHGWHRPPVAICQWE
Sbjct: 361 VLDDTGEDSHGVAVASWGAVLPRNEATESRAIAAIRIQMGMDAELSHGWHRPPVAICQWE 420

Query: 421 VARARLRASVDNAMRAASAVRALDQLAQKISNIVINDDVADRATRAVEILNDVVRPGRML 480
           VARARLRASVDNAMRAASAVRALDQLAQKISNIVINDDVADRATRAVEILNDVVRPGRML
Sbjct: 421 VARARLRASVDNAMRAASAVRALDQLAQKISNIVINDDVADRATRAVEILNDVVRPGRML 480

Query: 481 DFERLLEARRLADSANSDHSLLAMLYFPMDQRTAVLIPLAVPIVLPICKLVYEIAKITVF 540
           DFERLLEARRLADSANSDHSLLAMLYFPMDQRTAVLIPLAVPIVLPICKLVYEIAKITVF
Sbjct: 481 DFERLLEARRLADSANSDHSLLAMLYFPMDQRTAVLIPLAVPIVLPICKLVYEIAKITVF 540

Query: 541 WGYL 544
           WGYL
Sbjct: 541 WGYL 544
>ref|NP_149975.1| (NM_033198) phosphatidylinositol glycan, class S [Homo sapiens]
 dbj|BAB60853.1| (AB057723) phosphatidyl inositol glycan class S [Homo sapiens]
          Length = 555

 Score =  585 bits (1494), Expect = e-166
 Identities = 114/535 (21%), Positives = 206/535 (38%), Gaps = 53/535 (9%)

Query: 48  ASLSFVILVFFIGMPMWHFTTSTYRSNFPTFPSNQSITVPIRVIFVITSEDLRNDVKTLM 107
           A+L F  +   +G+P+W  TT TYR++ P    +    + +R++  +T    R  V  L 
Sbjct: 19  AALFFAAVAIVLGLPLWWKTTETYRASLPYSQISGLNALQLRLMVPVTVVFTRESVP-LD 77

Query: 108 NELEVNMT---KKEVVAPLDFTWTTQFIG--LRSFEHLEQDHSIQIEENKEFFDVFVALV 162
           ++ ++  T   ++E+          +F     R+ +H E+  S    +  E         
Sbjct: 78  DQEKLPFTVVHEREIPLKYKMKIKCRFQKAYRRALDHEEEALSSGSVQEAEAMLDEPQEQ 137

Query: 163 PEKEWT-HYSATRIQLGIGKWNFIQWPTKSEEKQKASTRVT-DLVSETLIDVPHLNSIVR 220
            E   T +  +    L          P ++   +    R   +++   ++ V    S+  
Sbjct: 138 AEGSLTVYVISEHSSLLPQDMMSYIGPKRTAVVRGIMHREAFNIIGRRIVQVAQAMSLTE 197

Query: 221 RDLRQKMQPWQIAALPLSHQKRLVWDSAPLATN--YHIQVIHLHENSEITPESLKEQEKT 278
             L   +      A  L   K       PL ++  Y I    L+ + +        +   
Sbjct: 198 DVLAAAL------ADHLPEDKWSAEKRRPLKSSLGYEITFSLLNPDPKSHDVYWDIEGAV 251

Query: 279 ARALRKFAEKVKEITNVEVSFEHLWDFEPTRGFLIKDVQERWALTHSAMEELVKKVDSQL 338
            R ++ F   +    N  V  + L+                + L   ++  ++  V+S+L
Sbjct: 252 RRYVQPFLNALGAAGNFSVDSQILYYAMLGVNPRFDSASSSYYLDMHSLPHVINPVESRL 311

Query: 339 QTSLQH-GAVLRFVVLET--VEPVIVLDDTGE---DSHGVAVASWGAVLPRN--EATESR 390
            +S      VL F++         + + D       ++      WG ++  N    T + 
Sbjct: 312 GSSAASLYPVLNFLLYVPELAHSPLYIQDKDGAPVATNAFHSPRWGGIMVYNVDSKTYNA 371

Query: 391 AIAAIRIQMGM---------DAELSHGWHRPPV------------AICQWEVARARLRAS 429
           ++  +R+++ M            L  G  +P +             +  WE+ R     S
Sbjct: 372 SVLPVRVEVDMVRVMEVFLAQLRLLFGIAQPQLPPKCLLSGPTSEGLMTWELDRLLWARS 431

Query: 430 VDNAMRAASAVRALDQLAQKISNIVINDDVAD---RATRAVEILNDVVRPGRMLD-FERL 485
           V+N   A + + +L QL  KISNIVI DDVA    +A  AV+   + +  G +   F   
Sbjct: 432 VENLATATTTLTSLAQLLGKISNIVIKDDVASEVYKAVAAVQKSAEELASGHLASAFVAS 491

Query: 486 LEARRLADSANSDHSLLAMLYFPMDQRTAVLIPLA----VPIVLPICKLVYEIAK 536
            EA   ++ A  D SLL +LYFP DQ+ A+ IPL     VPI+L + K+  E  K
Sbjct: 492 QEAVTSSELAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPILLSLVKIFLETRK 546
>gb|AAL27645.1| (AY061834) SD10476p [Drosophila melanogaster]
          Length = 536

 Score =  564 bits (1439), Expect = e-159
 Identities = 116/543 (21%), Positives = 215/543 (39%), Gaps = 92/543 (16%)

Query: 41  ERPYKLAASLSFVILVFFIGMPMWHFTTSTYRSNFPTFPSNQSITVPIRV---IFVITSE 97
           E  +++ A+L+F++++  IG+PMW  TT+ YR N P+        +PI+    + + T +
Sbjct: 18  EDKFRIGATLAFIVVIIGIGVPMWWRTTTVYRVNLPSTEILSLSKIPIKTAVQVAIYTQQ 77

Query: 98  DLRNDVKTLMNELEVNMTKKEVVAPLDFTWTTQFIGLRSFEHLEQDHSIQIEENKEFFDV 157
             R  +  L+ EL+   +  E+       W+ +F  L      ++ H+    E       
Sbjct: 78  TSRGQL--LIAELQNAFSDNEI-------WSVEFKQLSPTPMTQEAHTPAALEK------ 122

Query: 158 FVALVPEKEWTHYSATRIQLGIGKWNFIQWPTKSEEKQKASTRVTDLVSETLIDVPHLNS 217
              L+ E    H  +      +G + FI+WP   EE    + R   + S+T       N 
Sbjct: 123 ---LLLE---NHVQS------VGDFMFIEWPKLQEELLLTTERSALMRSDTP-----SNK 165

Query: 218 IVRRDLRQKMQPWQIAALPLSHQKRLVWDSAPLATNYHIQVIHLHENSEITPESLKEQEK 277
           I +    + +Q ++I  + LS  +R+   S      Y + V  L+    +T         
Sbjct: 166 IAQLLHAKILQTYRINQI-LSTDERMGAKSEAPQPAYDVIVSVLNPKPRLTHAKWNIAMA 224

Query: 278 TARALRKFAEKVKEITNVEVSFEHLWDFEPTRGFLIKDVQER------WALTHSAMEELV 331
               +  +  KV  ++N  V  +  W +       +K V+++      +AL  +A+  L+
Sbjct: 225 VKTYIEPWLAKVSGVSNYTVRSQ--WKYRVAIEADLKQVRDQSKLGRHYALQETALPHLL 282

Query: 332 KKVDSQLQTSLQHGAVLRFVVLET---VEPVIVLDDTGE-----DSHGVAVASWGAVLPR 383
             +   L  S      +  VV      + P+ + +   +     D        WG  +  
Sbjct: 283 TSIAQNLSASTTDKPAINLVVYIPPCHIAPLHIYNSKDQRLTRNDVDAFISPPWGGFIIA 342

Query: 384 N--------------------EATES--------RAIAAIRIQMGMDAELSHGWHRPPVA 415
           N                      T++          +  I  ++ M+           + 
Sbjct: 343 NPPEHVCLAAMSDQEAVPYHVSTTDNMQVMLDQLHKLLDISSELQMEGVKVVDIE--QLE 400

Query: 416 ICQWEVARARLRASVDNAMRAASAVRALDQLAQKISNIVINDDVADRATRAVEILNDVVR 475
             +WE      R+++ +   A+S +++L +L  +IS IVI+D+V    T +     D++ 
Sbjct: 401 PRRWEYEAYLRRSAIRHISTASSTLQSLIKLLDQISYIVIDDEVGAAITNSY---ADILA 457

Query: 476 PGRMLDFERLLEARRLADSA-------NSDHSLLAMLYFPMDQRTAVLIPLAVPIVLPIC 528
               L   RL +A  LA  A         D SLLA LYFP +Q+ A+ IPL +PI++P+ 
Sbjct: 458 AKAALLEHRLADASVLAKRAFVASERGFFDASLLAQLYFPDEQKYAIYIPLFLPIMVPVL 517

Query: 529 KLV 531
              
Sbjct: 518 SSF 520
>ref|NP_010722.1| (NC_001136) Glycosyl Phosphatidyl Inositol 17; Gpi17p
           [Saccharomyces cerevisiae]
 pir||S69714 hypothetical protein YDR434w - yeast (Saccharomyces cerevisiae)
 gb|AAB64866.1| (U33007) Ydr434wp; CAI: 0.14 [Saccharomyces cerevisiae]
          Length = 534

 Score =  509 bits (1297), Expect = e-143
 Identities = 75/528 (14%), Positives = 171/528 (32%), Gaps = 87/528 (16%)

Query: 52  FVILVFFIGMPMWHFTTSTYRSNFP--TFPSNQSITVP-IRVIFVITSEDLRNDVKTLMN 108
           FV +  F+G+P+W+  T+ YR++ P     S Q+     I ++  +  +        + +
Sbjct: 14  FVAIYLFLGVPLWYKLTTVYRASLPINYIESLQNNKFQDIHLVIPVYVKSDTYRFPDVHD 73

Query: 109 ELEVNMTKKEVVAPLDFTWTTQFIGLRSFEHLEQDHSIQIEENKEFFDVFVALVPEKEWT 168
            ++V                     +    + ++       +   + +    +  E    
Sbjct: 74  AIQV--------------------QVNHLLNSQEQRVPWSLQVLPYNETIEQMESEGNQF 113

Query: 169 HYSATRIQLGIGKWNFIQWPTKSEEKQKASTRVTDLVSETLIDVPHLNSIVRRDLRQKMQ 228
           H    ++   IG  +       ++E          L ++    V    ++V         
Sbjct: 114 HVVTLKLDEFIGYSSAYD----TKETLVYYDDAAVLSNDLPFFVAQ--TLVE-------H 160

Query: 229 PWQIAALPLSHQKRLVWDSAPLATNY----HIQVIHLHENSEITPESLKEQEKTARALRK 284
            +Q+    L+     V  +  +A +Y    H+ V  L  +    P + + +         
Sbjct: 161 TFQLEWTHLNKTCEGVSTNNDVAISYDPNIHLSVTLLSGDGN--PVAWEIEPTLTDYFSP 218

Query: 285 FAEKVKEITNVEVSFEHLWDFEPTRGFLIKDVQER--WALTHS-AMEELVKKVDSQLQTS 341
           F + +  + N  V    ++  +     L         + L+H+  + EL         +S
Sbjct: 219 FRKFLSPLVNFTVDSSIVYHNDLNLHSLNGSCTSVTWFDLSHTIDLSEL---------SS 269

Query: 342 LQHGA---VLRFVVLETVEP-----VIVLDDT---GE----DSHGVAVASWGAVLPR--- 383
           + +      L   ++          +  ++ T    E    D +   V  WG ++     
Sbjct: 270 MAYYPEDSALNLAIVFPSASSSPDGLAFINGTRISDEITTLDWNSYLVPQWGVIIINKMP 329

Query: 384 ---NEATESRAIAAIRIQMGMDAELSHGWHRPPVAICQ--WEVARARLRASVDNAMRAAS 438
              N       +  +  +   D     G       +      +   +    + N  +A  
Sbjct: 330 LKPNSVISEDYLEPMMYRFATDIFQLLGLTEGSQDLLSPYITIDSFKRLTILQNLDKATE 389

Query: 439 AVRALDQLAQKISNIVINDDVADRATRAVEILNDV----VRPGRMLD------FERLLEA 488
            + +L +L Q+   + I  +V+D    A+++   +      PG+  D           E 
Sbjct: 390 TLWSLVKLTQQFQGMSIPREVSDNVIEALDLRLQIIDLLNDPGKGGDIVWNNALHLSNEL 449

Query: 489 RRLADSANSDHSLLAMLYFPMDQRTAVLIPLAVPIVLPICKLVYEIAK 536
            +L + A  +  ++   +FP +   AV +PL  PI   +    Y + K
Sbjct: 450 VKLCEKAFFNGEMVQQNFFPQEHMIAVYLPLLGPISAVMFFGFYNVMK 497
>sp|Q10351|YDA9_SCHPO HYPOTHETICAL 63.9 KD PROTEIN C1F12.09 IN CHROMOSOME I
 pir||S67452 hypothetical protein SPAC1F12.09 - fission yeast
           (Schizosaccharomyces pombe)
 pir||T38067 hypothetical protein SPAC1F12.09 - fission yeast
           (Schizosaccharomyces pombe)
 emb|CAA93813.1| (Z69944) hypothetical protein [Schizosaccharomyces pombe]
          Length = 554

 Score =  489 bits (1248), Expect = e-137
 Identities = 64/461 (13%), Positives = 151/461 (31%), Gaps = 50/461 (10%)

Query: 119 VVAPLDFTWTTQFIGLRSFEHLEQDHSIQIEE--------------NKEFFDVFVALVPE 164
           ++  +   W T      S    + +++    +                      V  V E
Sbjct: 83  ILLAIPVWWKTTHYERSSLPFEDMENAPSTVQTHLRFSPTFRILDDKGNNLTKEVQKVLE 142

Query: 165 KEWTHYSATRIQLGIGKWNFIQWPTKSEEKQKASTRVTDLVSETLIDVPH------LNSI 218
            E   Y +  +++        +   +     +      + + +T    P       +N +
Sbjct: 143 AEPQIY-SYNLKVLEDDPVDYRIVLRESTDLQWFWDENNFIIDTPSKGPSELAILIVNCL 201

Query: 219 VRRDLRQKMQPWQIAALPLSHQKRL-VWDSAPLA--TNYHIQVIHLHENSEITPESLKEQ 275
                 Q M+ W       S  +         +     Y + +  L       P +   +
Sbjct: 202 WEAFSPQVMEVWSKFTRFSSTVEPSRAETKRTVQFSPQYRVLLSLLVGEGNHEPINWDIE 261

Query: 276 EKTARALRKFAEKVKEITNVEVSFEHLWDFEPTRGFLIKDVQERWALTHSAMEELVKKVD 335
               +      E++  +  + +  +  +  E      IKD  +++   H+ +  LV   +
Sbjct: 262 NAIQKYFNPLIEQLASLAKLNIETQIQYFVEDA-EAYIKD--DKFCTKHADLPNLVNNFE 318

Query: 336 SQLQTSLQ-HGAVLRFVVLETVEPVIVLDDTGEDSHGVAV-----ASWGAVLPRN-EATE 388
             L  S       + FV+      +  L    EDS+ +         WG++   N   TE
Sbjct: 319 KYLSFSPHIREPTIHFVLYVPSPQIQPLWLENEDSNIIPTNSMLLPQWGSITTINFNVTE 378

Query: 389 SRAIAAIRIQ-----MGMDAELSHGWHRPPVAICQWEV-ARARLRASVDNAMRAASAVRA 442
            + +  + ++     +  D  L  G +  PV+     +  R   +   ++ + A+  ++ 
Sbjct: 379 KKLLHDVDLKDYFRVISRDLLLLLGINDVPVSSLSATLADRLLRQRIAESCIEASDTLQN 438

Query: 443 LDQLAQKISNIVINDDVADRATRAVEILNDVVRPGRMLDFERLLEARRLADSAN------ 496
           L +L   + ++ +  ++       +  L+      + L    L EA   +++A       
Sbjct: 439 LAKLVHSMQSMAVPKEIQMYVKDTLLSLD---MAYKALSQNNLNEALSYSNNAFSKSQEA 495

Query: 497 -SDHSLLAMLYFPMDQRTAVLIPLAVPIVLPICKLVYEIAK 536
               S++  +YFP + +  +  PL  PI++P+     +  K
Sbjct: 496 LFHPSMVTTIYFPDESKYGIYAPLFAPILIPLLISFIKEVK 536
 Score = 38.9 bits (90), Expect = 0.21
 Identities = 19/65 (29%), Positives = 30/65 (45%), Gaps = 2/65 (3%)

Query: 29  EITQSFINYVHSERPYKLAASLSFVILVFFIGMPMWHFTTSTYRSNFPTFPS-NQSITVP 87
           E+ +S       E+  K  A LSF +++  + +P+W  TT   RS+ P     N   TV 
Sbjct: 56  EVERSNFQLNKPEKSLKRYALLSFYVIIL-LAIPVWWKTTHYERSSLPFEDMENAPSTVQ 114

Query: 88  IRVIF 92
             + F
Sbjct: 115 THLRF 119
>gb|AAL32707.1| (AY062629) Unknown protein [Arabidopsis thaliana]
          Length = 599

 Score =  367 bits (933), Expect = e-100
 Identities = 49/321 (15%), Positives = 101/321 (31%), Gaps = 40/321 (12%)

Query: 256 IQVIHLHENSEITPESLKEQEKTARALRKFAEKVKEITNVEVSFEHLWDFEPTRGFLIKD 315
           +    L+ N          Q      L    + +  I N+ V  + L+    +       
Sbjct: 260 LSFSLLNSNPRDWVYDWDFQRIDEALLAPVTKALAPIANITVESQVLYHTPKSSFSSWDK 319

Query: 316 VQERWALTHSAMEELVKKVDSQLQTSL---QHGAVLRFVVLETVEPVIVL-----DDTGE 367
             + +    S +   V   +  L TS        +L+FVV         L     +    
Sbjct: 320 KLQSYIFRTSDLPFFVNSNEWHLDTSAGASGRSKILQFVVYIPSGKECPLLLQLPNVEIS 379

Query: 368 DSHGVAVASWGAVLPRNEAT-ESRAIAAIRIQMGM------------DAELSHG------ 408
            ++G     WG V+  N    +  + +  R  + +                  G      
Sbjct: 380 KTNGFISPMWGGVIVWNPGNCDKDSESPSRNTISLQDLEQIVEIFLGQFRQLFGFKSEAK 439

Query: 409 ---------WHRPPVAICQWEVARARLRASVDNAMRAASAVRALDQLAQKISNIVINDDV 459
                             +WE+     + +  N    ++ + +L +L + +  ++I D++
Sbjct: 440 YTTGLGTYKILTSERGFTEWELDVLSRKHTCFNLHSCSTTLGSLSRLVRSLPRMIIKDEI 499

Query: 460 ADRATRAV--EILNDVVRP--GRMLDFERLLEARRLADSANSDHSLLAMLYFPMDQRTAV 515
            ++   ++    L        G         EAR LA++A    S++++ YF  +   AV
Sbjct: 500 GEQVKYSLKAAKLAQSNASLGGYSSSASSSREARSLAENAFFHPSIMSVSYFSYEHCFAV 559

Query: 516 LIPLAVPIVLPICKLVYEIAK 536
             P  +P+V  +        K
Sbjct: 560 YSPFFLPVVGHVVLAAVREWK 580
>gb|AAF56365.1| (AE003749) CG13644 gene product [Drosophila melanogaster]
          Length = 347

 Score =  356 bits (905), Expect = 6e-97
 Identities = 68/338 (20%), Positives = 125/338 (36%), Gaps = 56/338 (16%)

Query: 243 LVWDSAPLATNYHIQVIHLHENSEITPESLKEQEKTARALRKFAEKVKEITNVEVSFEHL 302
           +   S      Y + V  L+    +T             +  +  KV  ++N  V  +  
Sbjct: 1   MGAKSEAPQPAYDVIVSVLNPKPRLTHAKWNIAMAVKTYIEPWLAKVSGVSNYTVRSQ-- 58

Query: 303 WDFEPTRGFLIKDVQER------WALTHSAMEELVKKVDSQLQTSLQHGAVLRFVVLET- 355
           W +       +K V+++      +AL  +A+  L+  +   L  S      +  VV    
Sbjct: 59  WKYRVAIEADLKQVRDQSKLGRHYALQETALPHLLTSIAQNLSASTTDKPAINLVVYIPP 118

Query: 356 --VEPVIVLDDTGE-----DSHGVAVASWGAVLPRN--------------------EATE 388
             + P+ + +   +     D        WG  +  N                      T+
Sbjct: 119 CHIAPLHIYNSKDQRLTRNDVDAFISPPWGGFIIANPPEHVCLAAMSDQEAVPYHVSTTD 178

Query: 389 S--------RAIAAIRIQMGMDAELSHGWHRPPVAICQWEVARARLRASVDNAMRAASAV 440
           +          +  I  ++ M+           +   +WE      R+++ +   A+S +
Sbjct: 179 NMQVMLDQLHKLLDISSELQMEGVKVVDIE--QLEPRRWEYEAYLRRSAIRHISTASSTL 236

Query: 441 RALDQLAQKISNIVINDDVADRATRAVEILNDVVRPGRMLDFERLLEARRLADSA----- 495
           ++L +L  +IS IVI+D+V    T +     D++     L   RL +A  LA  A     
Sbjct: 237 QSLIKLLDQISYIVIDDEVGAAITNSY---ADILAAKAALLEHRLADASVLAKRAFVASE 293

Query: 496 --NSDHSLLAMLYFPMDQRTAVLIPLAVPIVLPICKLV 531
               D SLLA LYFP +Q+ A+ IPL +PI++P+    
Sbjct: 294 RGFFDASLLAQLYFPDEQKYAIYIPLFLPIMVPVLSSF 331
>ref|NP_187374.1| (NM_111598) unknown protein [Arabidopsis thaliana]
 gb|AAF20228.1|AC012395_15 (AC012395) unknown protein [Arabidopsis thaliana]
          Length = 605

 Score =  338 bits (859), Expect = 2e-91
 Identities = 45/302 (14%), Positives = 94/302 (30%), Gaps = 40/302 (13%)

Query: 256 IQVIHLHENSEITPESLKEQEKTARALRKFAEKVKEITNVEVSFEHLWDFEPTRGFLIKD 315
           +    L+ N          Q      L    + +  I N+ V  + L+    +       
Sbjct: 283 LSFSLLNSNPRDWVYDWDFQRIDEALLAPVTKALAPIANITVESQVLYHTPKSSFSSWDK 342

Query: 316 VQERWALTHSAMEELVKKVDSQLQTSL---QHGAVLRFVVLETVEPVIVL-----DDTGE 367
             + +    S +   V   +  L TS        +L+FVV         L     +    
Sbjct: 343 KLQSYIFRTSDLPFFVNSNEWHLDTSAGASGRSKILQFVVYIPSGKECPLLLQLPNVEIS 402

Query: 368 DSHGVAVASWGAVLPRNEAT-ESRAIAAIRIQMGM------------DAELSHG------ 408
            ++G     WG V+  N    +  + +  R  + +                  G      
Sbjct: 403 KTNGFISPMWGGVIVWNPGNCDKDSESPSRNTISLQDLEQIVEIFLGQFRQLFGFKSEAK 462

Query: 409 ---------WHRPPVAICQWEVARARLRASVDNAMRAASAVRALDQLAQKISNIVINDDV 459
                             +WE+     + +  N    ++ + +L +L + +  ++I D++
Sbjct: 463 YTTGLGTYKILTSERGFTEWELDVLSRKHTCFNLHSCSTTLGSLSRLVRSLPRMIIKDEI 522

Query: 460 ADRATRAV--EILNDVVRP--GRMLDFERLLEARRLADSANSDHSLLAMLYFPMDQRTAV 515
            ++   ++    L        G         EAR LA++A    S++++ YF  +   AV
Sbjct: 523 GEQVKYSLKAAKLAQSNASLGGYSSSASSSREARSLAENAFFHPSIMSVSYFSYEHCFAV 582

Query: 516 LI 517
             
Sbjct: 583 YS 584
>ref|XP_091596.1| (XM_091596) similar to phosphatidylinositol glycan, class S (H.
           sapiens) [Homo sapiens]
          Length = 204

 Score =  230 bits (582), Expect = 5e-59
 Identities = 27/186 (14%), Positives = 54/186 (28%), Gaps = 12/186 (6%)

Query: 122 PLDFTWTTQFIG--LRSFEHLEQDHSIQIEENKEFFDVFVALVPEKEWT-HYSATRIQLG 178
           P       +F     R+ +H E+  S    +  E          E   T +  +    L 
Sbjct: 20  PNKMKIKCRFQKAYRRALDHEEEALSSGSVQEAEAMLDEPQEQAEGSLTVYVISEHSSLL 79

Query: 179 IGKWNFIQWPTKSEEKQKASTRVT-DLVSETLIDVPHLNSIVRRDLRQKMQPWQIAALPL 237
                    P ++   +    R   +++   ++ V    S+    L   +      A  L
Sbjct: 80  PQDMMSYIGPKRTAVVRGIMHREAFNIIGRRIVQVAQAMSLTEDVLAAAL------ADHL 133

Query: 238 SHQKRLVWDSAPLATN--YHIQVIHLHENSEITPESLKEQEKTARALRKFAEKVKEITNV 295
              K       PL ++  Y I    L+ + +        +    R ++ F   +    N 
Sbjct: 134 PEDKWSAEKRRPLKSSLGYEITFSLLNPDPKSHDVYWDIEGAVRRYVQPFLNALGAAGNF 193

Query: 296 EVSFEH 301
            V  + 
Sbjct: 194 SVDSQV 199
>gb|AAF56366.1| (AE003749) CG6031 gene product [Drosophila melanogaster]
          Length = 164

 Score =  186 bits (470), Expect = 7e-46
 Identities = 41/171 (23%), Positives = 76/171 (43%), Gaps = 30/171 (17%)

Query: 41  ERPYKLAASLSFVILVFFIGMPMWHFTTSTYRSNFPTFPSNQSITVPIRV---IFVITSE 97
           E  +++ A+L+F++++  IG+PMW  TT+ YR N P+        +PI+    + + T +
Sbjct: 18  EDKFRIGATLAFIVVIIGIGVPMWWRTTTVYRVNLPSTEILSLSKIPIKTAVQVAIYTQQ 77

Query: 98  DLRNDVKTLMNELEVNMTKKEVVAPLDFTWTTQFIGLRSFEHLEQDHSIQIEENKEFFDV 157
             R  +  L+ EL+   +  E+       W+ +F  L      ++ H+    E       
Sbjct: 78  TSRGQL--LIAELQNAFSDNEI-------WSVEFKQLSPTPMTQEAHTPAALEK------ 122

Query: 158 FVALVPEKEWTHYSATRIQLGIGKWNFIQWPTKSEEKQKASTRVTDLVSET 208
              L+ E    H  +      +G + FI+WP   EE    + R   + S+T
Sbjct: 123 ---LLLE---NHVQS------VGDFMFIEWPKLQEELLLTTERSALMRSDT 161
>dbj|BAB70938.1| (AK055512) unnamed protein product [Homo sapiens]
          Length = 184

 Score =  160 bits (403), Expect = 4e-38
 Identities = 54/172 (31%), Positives = 78/172 (44%), Gaps = 29/172 (16%)

Query: 394 AIRIQMGM---------DAELSHGWHRPPV------------AICQWEVARARLRASVDN 432
            +R+++ M            L  G  +P +             +  WE+ R     SV+N
Sbjct: 4   PVRVEVDMVRVMEVFLAQLRLLFGIAQPQLPPKCLLSGPTSEGLMTWELDRLLWARSVEN 63

Query: 433 AMRAASAVRALDQLAQKISNIVINDDVAD---RATRAVEILNDVVRPGRMLD-FERLLEA 488
              A + + +L QL  KISNIVI DDVA    +A  AV+   + +  G +   F    E 
Sbjct: 64  LATATTTLTSLAQLLGKISNIVIKDDVASEVYKAVAAVQKSAEELASGHLASAFVASQEV 123

Query: 489 RRLADSANSDHSLLAMLYFPMDQRTAVLIPLA----VPIVLPICKLVYEIAK 536
              ++ A  D SLL +LYFP DQ+  + IPL     VPI+L + KL  E  K
Sbjct: 124 VTSSELAFFDPSLLHLLYFPDDQKFPIYIPLFLPMAVPILLSLVKLFLETRK 175
>ref|NP_540827.1| (NC_003317) RECOMBINATION PROTEIN RECR [Brucella melitensis]
 gb|AAL53091.1| (AE009624) RECOMBINATION PROTEIN RECR [Brucella melitensis]
          Length = 224

 Score = 33.4 bits (76), Expect = 9.4
 Identities = 12/90 (13%), Positives = 26/90 (28%), Gaps = 7/90 (7%)

Query: 240 QKRLVWDSAPLATNYHIQVIHLHENSEITPESLKEQEKTARALRKFAEKVKEITNVEVSF 299
               +  +  +   YH+    L     I P+ L  +    R      ++V    N  V  
Sbjct: 116 DLWALERADTMNVRYHVLGGRLSPLDGIGPDDLNIKGLVERVASGAIKEVILAVNATVEG 175

Query: 300 EHLWDFEPTRGFLIKDVQERWALTHSAMEE 329
           +    +       I D    + +  + +  
Sbjct: 176 QTTAHY-------ITDQLSNFDVRVTRLAH 198
CPU time:   101.90 user secs.	    2.21 sys. secs	  104.11 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.312    0.166    0.450 

Gapped
Lambda     K      H
   0.270   0.0583    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 268430665
Number of Sequences: 887402
Number of extensions: 10658701
Number of successful extensions: 38060
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 37963
Number of HSP's gapped (non-prelim): 26
length of query: 544
length of database: 277,845,442
effective HSP length: 59
effective length of query: 485
effective length of database: 225,488,724
effective search space: 109362031140
effective search space used: 109362031140
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.1 bits)
S2: 76 (33.7 bits)