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GPI Anchor Biosynthesis Report: EAA07616.1(PIG-S family, Anopheles gambiae str. PEST)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 
         (590 letters)

Database: nr
           1,315,992 sequences; 421,297,171 total letters

Searching..................................................

Converged !!!


Results of PSI-Blast iteration 1

Distribution of 20 Blast Hits on the Query Sequence


Sequences with E-value BETTER than threshold (0.002)

gb|EAA07616.1| agCP1277 [Anopheles gambiae str. PEST] 891 0.0
ref|NP_733060.1| CG31120-PB [Drosophila melanogaster] >gi|169020... 704 0.0
ref|XP_203412.1| similar to phosphatidylinositol glycan class S ... 670 0.0
ref|NP_149975.1| phosphatidylinositol glycan class S [Homo sapie... 669 0.0
dbj|BAC11615.1| unnamed protein product [Homo sapiens] 661 0.0
ref|XP_220633.1| similar to phosphatidylinositol glycan class S ... 566 e-160
ref|NP_187374.2| unknown protein; protein id: At3g07180.1, suppo... 444 e-123
gb|AAF20228.1|AC012395_15 unknown protein [Arabidopsis thaliana] 398 e-109
ref|NP_010722.1| Glycosyl Phosphatidyl Inositol 17; Gpi17p [Sacc... 393 e-108
ref|NP_594335.1| hypothetical protein [Schizosaccharomyces pombe... 349 1e-94
dbj|BAB92563.1| P0497A05.6 [Oryza sativa (japonica cultivar-group)] 326 1e-87
ref|XP_142436.2| similar to phosphatidylinositol glycan class S ... 278 4e-73
ref|XP_194600.1| similar to phosphatidylinositol glycan class S ... 171 5e-41
ref|NP_501852.1| Putative endoplasmic reticulum membrane protein... 141 6e-32
ref|XP_220635.1| similar to phosphatidylinositol glycan class S ... 126 1e-27
Alignments
>gb|EAA07616.1| agCP1277 [Anopheles gambiae str. PEST]
          Length = 590

 Score =  891 bits (2278), Expect = 0.0
 Identities = 590/590 (100%), Positives = 590/590 (100%)

Query: 1   TVSMSAKTPSEETKAQTKASSGDMATDKRRVQQDDDASGEQCEAGSSSAAREDAAIHIYA 60
           TVSMSAKTPSEETKAQTKASSGDMATDKRRVQQDDDASGEQCEAGSSSAAREDAAIHIYA
Sbjct: 1   TVSMSAKTPSEETKAQTKASSGDMATDKRRVQQDDDASGEQCEAGSSSAAREDAAIHIYA 60

Query: 61  TIAFIVIIIVIGVPMWWKTTEVYRVSLPYSEIRALNEDPIKATFRLGLYCQSAERREILS 120
           TIAFIVIIIVIGVPMWWKTTEVYRVSLPYSEIRALNEDPIKATFRLGLYCQSAERREILS
Sbjct: 61  TIAFIVIIIVIGVPMWWKTTEVYRVSLPYSEIRALNEDPIKATFRLGLYCQSAERREILS 120

Query: 121 FELRKKFEQNFVFELDLQLLSLDADTLSTIKTPARLEAELQKHYPSTLGHFLFIEWPKLD 180
           FELRKKFEQNFVFELDLQLLSLDADTLSTIKTPARLEAELQKHYPSTLGHFLFIEWPKLD
Sbjct: 121 FELRKKFEQNFVFELDLQLLSLDADTLSTIKTPARLEAELQKHYPSTLGHFLFIEWPKLD 180

Query: 181 DDILVTSDRTAFISESASSLKAYQVLSSWLLQEYKLKAILGSRDAQQTLMGRQLRLNSAP 240
           DDILVTSDRTAFISESASSLKAYQVLSSWLLQEYKLKAILGSRDAQQTLMGRQLRLNSAP
Sbjct: 181 DDILVTSDRTAFISESASSLKAYQVLSSWLLQEYKLKAILGSRDAQQTLMGRQLRLNSAP 240

Query: 241 LQAQYEVLITVLNPRPELQKVHWNARAAAENYIEPFLSELSGITNFTIKTQWIYQVGLEG 300
           LQAQYEVLITVLNPRPELQKVHWNARAAAENYIEPFLSELSGITNFTIKTQWIYQVGLEG
Sbjct: 241 LQAQYEVLITVLNPRPELQKVHWNARAAAENYIEPFLSELSGITNFTIKTQWIYQVGLEG 300

Query: 301 AGAQPKQVPDDSKLGRHYALAEDSLPHIITSLEKKLGTQITDNPCIHLVVYVPPCAQAPL 360
           AGAQPKQVPDDSKLGRHYALAEDSLPHIITSLEKKLGTQITDNPCIHLVVYVPPCAQAPL
Sbjct: 301 AGAQPKQVPDDSKLGRHYALAEDSLPHIITSLEKKLGTQITDNPCIHLVVYVPPCAQAPL 360

Query: 361 RIYRKDGQRAAPLASNVEAFTSAKWGGIVFANPAEATCVRYMETEQFSDVYIHAQDVMPV 420
           RIYRKDGQRAAPLASNVEAFTSAKWGGIVFANPAEATCVRYMETEQFSDVYIHAQDVMPV
Sbjct: 361 RIYRKDGQRAAPLASNVEAFTSAKWGGIVFANPAEATCVRYMETEQFSDVYIHAQDVMPV 420

Query: 421 LLYQLRKIFDLENNTPLLDATLVPYSTIEPRAWEVDTFMRTNTIYLVHSATTTLQSLIQL 480
           LLYQLRKIFDLENNTPLLDATLVPYSTIEPRAWEVDTFMRTNTIYLVHSATTTLQSLIQL
Sbjct: 421 LLYQLRKIFDLENNTPLLDATLVPYSTIEPRAWEVDTFMRTNTIYLVHSATTTLQSLIQL 480

Query: 481 LGGIEYIVINDEVGAAIQTAHRKIVAAKQQLRAGRLQQAALLAREAYTSAERAFFDPSML 540
           LGGIEYIVINDEVGAAIQTAHRKIVAAKQQLRAGRLQQAALLAREAYTSAERAFFDPSML
Sbjct: 481 LGGIEYIVINDEVGAAIQTAHRKIVAAKQQLRAGRLQQAALLAREAYTSAERAFFDPSML 540

Query: 541 ALLYFPDEQKYAIYIPLFLPIMIPVVFSFNTILKYFRKRKATTATKAKEE 590
           ALLYFPDEQKYAIYIPLFLPIMIPVVFSFNTILKYFRKRKATTATKAKEE
Sbjct: 541 ALLYFPDEQKYAIYIPLFLPIMIPVVFSFNTILKYFRKRKATTATKAKEE 590
>ref|NP_733060.1| CG31120-PB [Drosophila melanogaster]
 gb|AAL27645.1| SD10476p [Drosophila melanogaster]
 gb|AAF56366.2| CG31120-PB [Drosophila melanogaster]
          Length = 536

 Score =  704 bits (1799), Expect = 0.0
 Identities = 233/531 (43%), Positives = 344/531 (63%), Gaps = 14/531 (2%)

Query: 51  REDAAIHIYATIAFIVIIIVIGVPMWWKTTEVYRVSLPYSEIRALNEDPIKATFRLGLYC 110
           +++    I AT+AFIV+II IGVPMWW+TT VYRV+LP +EI +L++ PIK   ++ +Y 
Sbjct: 16  KDEDKFRIGATLAFIVVIIGIGVPMWWRTTTVYRVNLPSTEILSLSKIPIKTAVQVAIYT 75

Query: 111 QSAERREILSFELRKKFEQNFVFELDLQLLSLDADTLSTIKTPARLEAELQKHYPSTLGH 170
           Q   R ++L  EL+  F  N ++ ++ + LS    T     TPA LE  L +++  ++G 
Sbjct: 76  QQTSRGQLLIAELQNAFSDNEIWSVEFKQLSPTPMT-QEAHTPAALEKLLLENHVQSVGD 134

Query: 171 FLFIEWPKLDDDILVTSDRTAFISESASSLKAYQVLSSWLLQEYKLKAILGSRDAQQTLM 230
           F+FIEWPKL +++L+T++R+A +     S K  Q+L + +LQ Y++  IL + +     M
Sbjct: 135 FMFIEWPKLQEELLLTTERSALMRSDTPSNKIAQLLHAKILQTYRINQILSTDER----M 190

Query: 231 GRQLRLNSAPLQAQYEVLITVLNPRPELQKVHWNARAAAENYIEPFLSELSGITNFTIKT 290
           G +   + AP Q  Y+V+++VLNP+P L    WN   A + YIEP+L+++SG++N+T+++
Sbjct: 191 GAK---SEAP-QPAYDVIVSVLNPKPRLTHAKWNIAMAVKTYIEPWLAKVSGVSNYTVRS 246

Query: 291 QWIYQVGLEGAGAQPKQVPDDSKLGRHYALAEDSLPHIITSLEKKLGTQITDNPCIHLVV 350
           QW Y+V +E   A  KQV D SKLGRHYAL E +LPH++TS+ + L    TD P I+LVV
Sbjct: 247 QWKYRVAIE---ADLKQVRDQSKLGRHYALQETALPHLLTSIAQNLSASTTDKPAINLVV 303

Query: 351 YVPPCAQAPLRIYRKDGQRAAPLASNVEAFTSAKWGGIVFANPAEATCVRYMETEQFSDV 410
           Y+PPC  APL IY    QR     ++V+AF S  WGG + ANP E  C+  M  ++    
Sbjct: 304 YIPPCHIAPLHIYNSKDQRLT--RNDVDAFISPPWGGFIIANPPEHVCLAAMSDQEAVPY 361

Query: 411 YIHAQDVMPVLLYQLRKIFDLENNTPLLDATLVPYSTIEPRAWEVDTFMRTNTIYLVHSA 470
           ++   D M V+L QL K+ D+ +   +    +V    +EPR WE + ++R + I  + +A
Sbjct: 362 HVSTTDNMQVMLDQLHKLLDISSELQMEGVKVVDIEQLEPRRWEYEAYLRRSAIRHISTA 421

Query: 471 TTTLQSLIQLLGGIEYIVINDEVGAAIQTAHRKIVAAKQQLRAGRLQQAALLAREAYTSA 530
           ++TLQSLI+LL  I YIVI+DEVGAAI  ++  I+AAK  L   RL  A++LA+ A+ ++
Sbjct: 422 SSTLQSLIKLLDQISYIVIDDEVGAAITNSYADILAAKAALLEHRLADASVLAKRAFVAS 481

Query: 531 ERAFFDPSMLALLYFPDEQKYAIYIPLFLPIMIPVVFSFNTILKYFRKRKA 581
           ER FFD S+LA LYFPDEQKYAIYIPLFLPIM+PV+ SFN +    + R+ 
Sbjct: 482 ERGFFDASLLAQLYFPDEQKYAIYIPLFLPIMVPVLSSFNMLRGVLQARRK 532
>ref|XP_203412.1| similar to phosphatidylinositol glycan class S [Homo sapiens] [Mus
           musculus]
          Length = 555

 Score =  670 bits (1712), Expect = 0.0
 Identities = 184/571 (32%), Positives = 279/571 (48%), Gaps = 55/571 (9%)

Query: 44  AGSSSAAREDAAIHIYATIAFIVIIIVIGVPMWWKTTEVYRVSLPYSEIRALNEDPIKAT 103
           A  ++A   +      + + F  + I++G+P+WWKTTE YR  LPYS+I  LN       
Sbjct: 3   AAGAAATDLEVVRGKRSALFFAAVAILLGLPLWWKTTETYRAPLPYSDISGLNA----LL 58

Query: 104 FRLGLYCQSAERREILSFELRKKFEQNFVFELDLQLL-------------------SLDA 144
            RL +       R+ +  + ++K     V E ++ L                      +A
Sbjct: 59  LRLMVPVTVVFTRDSVPLDDQEKLPFTVVHEREIPLKYKMKIKCRFQKAYRRALEHEEEA 118

Query: 145 DTLSTIKTPARLEAELQKHYPSTLGHFLFIEWPK-LDDDIL--VTSDRTA----FISESA 197
            +L ++     + AE +K    +L  ++  E    L  D++  +  +RTA     I   A
Sbjct: 119 LSLGSVHEAEAMLAEPEKQAEGSLTVYVISEHSSLLPQDMMSYIGPERTAVVRGLIHREA 178

Query: 198 SSL---KAYQVLSSWLLQEYKLKAILGSRDAQQTLMGRQLRLNSAPLQAQ--YEVLITVL 252
            ++   +  QV  +  L E  L A L     +      + R    PL++   YE+  ++L
Sbjct: 179 FNIIGRRIVQVAQAMSLTEDVLAAALADHLPEDKWSSDKRR----PLKSSLGYEITFSLL 234

Query: 253 NPRPELQKVHWNARAAAENYIEPFLSELSGITNFTIKTQWIYQVGLEGAGAQPKQVPDDS 312
           NP P+   VHW+   A + +++PFL+ LS   NF++ +Q +Y   L   G  P+  P  S
Sbjct: 235 NPDPKSHDVHWDIEGAVQRFVQPFLNRLSVAGNFSVDSQILYYAML---GVNPRFDPASS 291

Query: 313 KLGRHYALAEDSLPHIITSLEKKLGTQITD-NPCIHLVVYVPPCAQAPLRIYRKDGQRAA 371
                Y+LA  SLPH+I  +E +LG+      P +H ++YVP  A +PL I  KDG   A
Sbjct: 292 S----YSLAMHSLPHVINPVESRLGSSAASLYPVLHFLLYVPELAHSPLYIQDKDG---A 344

Query: 372 PLASNVEAFTSAKWGGIVFANPAEATCVRYMETEQFSDVYIHAQDVMPVLLYQLRKIFDL 431
           P+A+N  AF S +WGGI+  N        Y  +E    V +    VM V L QLR +F +
Sbjct: 345 PVATN--AFHSPRWGGIMVYNVDPKI---YNASELPVRVEVDMVRVMEVFLAQLRLLFGI 399

Query: 432 ENNTPLLDATLVPYSTIEPRAWEVDTFMRTNTIYLVHSATTTLQSLIQLLGGIEYIVIND 491
                     L    +     WE+D  +   ++  + +ATTTL SL QLLG I  IVI D
Sbjct: 400 AQPQVPPKCLLSGPKSEGLMTWELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKD 459

Query: 492 EVGAAIQTAHRKIVAAKQQLRAGRLQQAALLAREAYTSAERAFFDPSMLALLYFPDEQKY 551
           +V + +  A   +  A + L  G L  A   ++EA TS+ERAFFDPS+L LLYFPD+QK+
Sbjct: 460 DVASEVYRAVAAVQKAAKALALGHLSSAFAASQEAVTSSERAFFDPSLLHLLYFPDDQKF 519

Query: 552 AIYIPLFLPIMIPVVFSFNTILKYFRKRKAT 582
           AIYIPLFLP+ +P++ S   I +  RK    
Sbjct: 520 AIYIPLFLPMAVPILLSLVKIFQETRKSWKK 550
>ref|NP_149975.1| phosphatidylinositol glycan class S [Homo sapiens]
 sp|Q96S52|PIGS_HUMAN GPI transamidase component PIG-S (Phosphatidylinositol-glycan
           biosynthesis, class S protein)
 dbj|BAB60853.1| phosphatidyl inositol glycan class S [Homo sapiens]
          Length = 555

 Score =  669 bits (1708), Expect = 0.0
 Identities = 180/567 (31%), Positives = 276/567 (47%), Gaps = 55/567 (9%)

Query: 44  AGSSSAAREDAAIHIYATIAFIVIIIVIGVPMWWKTTEVYRVSLPYSEIRALNEDPIKAT 103
           A  ++A   + A    A + F  + IV+G+P+WWKTTE YR SLPYS+I  LN       
Sbjct: 3   AAGAAATHLEVARGKRAALFFAAVAIVLGLPLWWKTTETYRASLPYSQISGLNA----LQ 58

Query: 104 FRLGLYCQSAERREILSFELRKKFEQNFVFELDLQLL-------------------SLDA 144
            RL +       RE +  + ++K     V E ++ L                      +A
Sbjct: 59  LRLMVPVTVVFTRESVPLDDQEKLPFTVVHEREIPLKYKMKIKCRFQKAYRRALDHEEEA 118

Query: 145 DTLSTIKTPARLEAELQKHYPSTLGHFLFIEWPK-LDDDIL--VTSDRTA----FISESA 197
            +  +++    +  E Q+    +L  ++  E    L  D++  +   RTA     +   A
Sbjct: 119 LSSGSVQEAEAMLDEPQEQAEGSLTVYVISEHSSLLPQDMMSYIGPKRTAVVRGIMHREA 178

Query: 198 SSL---KAYQVLSSWLLQEYKLKAILGSRDAQQTLMGRQLRLNSAPLQAQ--YEVLITVL 252
            ++   +  QV  +  L E  L A L     +      + R    PL++   YE+  ++L
Sbjct: 179 FNIIGRRIVQVAQAMSLTEDVLAAALADHLPEDKWSAEKRR----PLKSSLGYEITFSLL 234

Query: 253 NPRPELQKVHWNARAAAENYIEPFLSELSGITNFTIKTQWIYQVGLEGAGAQPKQVPDDS 312
           NP P+   V+W+   A   Y++PFL+ L    NF++ +Q +Y   L   G  P+     S
Sbjct: 235 NPDPKSHDVYWDIEGAVRRYVQPFLNALGAAGNFSVDSQILYYAML---GVNPRFDSASS 291

Query: 313 KLGRHYALAEDSLPHIITSLEKKLGTQITD-NPCIHLVVYVPPCAQAPLRIYRKDGQRAA 371
                Y L   SLPH+I  +E +LG+      P ++ ++YVP  A +PL I  KDG   A
Sbjct: 292 S----YYLDMHSLPHVINPVESRLGSSAASLYPVLNFLLYVPELAHSPLYIQDKDG---A 344

Query: 372 PLASNVEAFTSAKWGGIVFANPAEATCVRYMETEQFSDVYIHAQDVMPVLLYQLRKIFDL 431
           P+A+N  AF S +WGGI+  N    T   Y  +     V +    VM V L QLR +F +
Sbjct: 345 PVATN--AFHSPRWGGIMVYNVDSKT---YNASVLPVRVEVDMVRVMEVFLAQLRLLFGI 399

Query: 432 ENNTPLLDATLVPYSTIEPRAWEVDTFMRTNTIYLVHSATTTLQSLIQLLGGIEYIVIND 491
                     L   ++     WE+D  +   ++  + +ATTTL SL QLLG I  IVI D
Sbjct: 400 AQPQLPPKCLLSGPTSEGLMTWELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKD 459

Query: 492 EVGAAIQTAHRKIVAAKQQLRAGRLQQAALLAREAYTSAERAFFDPSMLALLYFPDEQKY 551
           +V + +  A   +  + ++L +G L  A + ++EA TS+E AFFDPS+L LLYFPD+QK+
Sbjct: 460 DVASEVYKAVAAVQKSAEELASGHLASAFVASQEAVTSSELAFFDPSLLHLLYFPDDQKF 519

Query: 552 AIYIPLFLPIMIPVVFSFNTILKYFRK 578
           AIYIPLFLP+ +P++ S   I    RK
Sbjct: 520 AIYIPLFLPMAVPILLSLVKIFLETRK 546
>dbj|BAC11615.1| unnamed protein product [Homo sapiens]
          Length = 555

 Score =  661 bits (1689), Expect = 0.0
 Identities = 179/567 (31%), Positives = 274/567 (47%), Gaps = 55/567 (9%)

Query: 44  AGSSSAAREDAAIHIYATIAFIVIIIVIGVPMWWKTTEVYRVSLPYSEIRALNEDPIKAT 103
           A  ++A   + A    A + F  + IV+G+P+WWKTTE YR SLPYS+I  LN       
Sbjct: 3   AAGAAATHLEVARGKRAALFFAAVAIVLGLPLWWKTTETYRASLPYSQISGLNA----LQ 58

Query: 104 FRLGLYCQSAERREILSFELRKKFEQNFVFELDLQLL-------------------SLDA 144
            RL +       RE +  + ++K     V E ++ L                      +A
Sbjct: 59  LRLMVPVTVVFTRESVPLDDQEKLPFTVVHEREIPLKYKMKIKCRFQKAYRRALDHEEEA 118

Query: 145 DTLSTIKTPARLEAELQKHYPSTLGHFLFIEWPK-LDDDIL--VTSDRTA----FISESA 197
            +  +++    +  E Q+    +L  ++  E    L  D++  +   RTA     +   A
Sbjct: 119 LSSGSVQEAEAMLDEPQEQAEGSLTVYVISEHSSLLPQDMMSYIGPKRTAVVRGIMHREA 178

Query: 198 SSL---KAYQVLSSWLLQEYKLKAILGSRDAQQTLMGRQLRLNSAPLQAQ--YEVLITVL 252
            ++   +  QV  +  L E  L A L     +      + R    PL++   YE+  ++L
Sbjct: 179 FNIIGRRIVQVAQAMSLTEDVLAAALADHLPEDKWSAEKRR----PLKSSLGYEITFSLL 234

Query: 253 NPRPELQKVHWNARAAAENYIEPFLSELSGITNFTIKTQWIYQVGLEGAGAQPKQVPDDS 312
           NP P+   V+W+   A   Y++P L+ L    NF++ +Q +Y   L   G  P+     S
Sbjct: 235 NPDPKSHDVYWDIEGAVRRYVQPSLNALGAAGNFSVDSQILYYAML---GVNPRFDSASS 291

Query: 313 KLGRHYALAEDSLPHIITSLEKKLGTQITD-NPCIHLVVYVPPCAQAPLRIYRKDGQRAA 371
                Y L   SLPH+I  +E +LG+      P ++ ++YVP  A +PL I  KDG   A
Sbjct: 292 S----YYLDMHSLPHVINPVESRLGSSAASLYPVLNFLLYVPELAHSPLYIQDKDG---A 344

Query: 372 PLASNVEAFTSAKWGGIVFANPAEATCVRYMETEQFSDVYIHAQDVMPVLLYQLRKIFDL 431
           P+A+N  AF S +WGGI+  N    T   Y  +     V +    VM V L QLR +F +
Sbjct: 345 PVATN--AFHSPRWGGIMVYNVDSKT---YNASVLPVRVEVDMVRVMEVFLAQLRLLFGI 399

Query: 432 ENNTPLLDATLVPYSTIEPRAWEVDTFMRTNTIYLVHSATTTLQSLIQLLGGIEYIVIND 491
                     L   ++     WE+D  +   ++  + +ATTTL SL QLLG I  IVI D
Sbjct: 400 AQPQLPPKCLLSGPTSEGLMTWELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKD 459

Query: 492 EVGAAIQTAHRKIVAAKQQLRAGRLQQAALLAREAYTSAERAFFDPSMLALLYFPDEQKY 551
           +V + +  A   +  + ++L +G L  A + ++EA TS+E AFFDPS+L LLYFPD QK+
Sbjct: 460 DVASEVYKAVAAVQKSAEELASGHLASAFVASQEAVTSSELAFFDPSLLHLLYFPDYQKF 519

Query: 552 AIYIPLFLPIMIPVVFSFNTILKYFRK 578
           AIYIPLFLP+ +P++ S   I    RK
Sbjct: 520 AIYIPLFLPMAVPILLSLVKIFLETRK 546
>ref|XP_220633.1| similar to phosphatidylinositol glycan class S [Homo sapiens]
           [Rattus norvegicus]
          Length = 496

 Score =  566 bits (1445), Expect = e-160
 Identities = 155/486 (31%), Positives = 233/486 (47%), Gaps = 45/486 (9%)

Query: 120 SFELRKKFEQNFVFELDLQLLSLDADTLSTIKTPARLEAELQKHYPSTLGHFLFIEWPK- 178
             +++ +F++ +   L+ +  +L   +L ++     L AE +K    +L  ++  E    
Sbjct: 28  KMKIKCRFQKAYRRALEHEEEAL---SLGSVHEAEALLAEPEKQAEGSLTVYVISEHSSL 84

Query: 179 LDDDIL--VTSDRTA----FISESASSL---KAYQVLSSWLLQEYKLKAILGSRDAQQTL 229
           L  D++  +  +RTA     I   A ++   +   V  +  L E  L A L     +   
Sbjct: 85  LPQDMMSYIGPERTAIVRGMIHREAFNIIGRRIIHVAQAMSLTEDVLAAALADHLPEDKW 144

Query: 230 MGRQLRLNSAPLQAQ--YEVLITVLNPRPELQKVHWNARAAAEN----YIEP------FL 277
              + R    PL++   YE+  ++LNP P+   VHW+           ++EP      FL
Sbjct: 145 SSDKRR----PLKSSLGYEITFSLLNPDPKSHDVHWDIGGGCPTLCAAFLEPPQRCRQFL 200

Query: 278 SELSGITNFTIKTQWIYQVGLEGAGAQPKQVPDDSKLGRHYALAEDSLPHIITSLEKKLG 337
             LS I       Q +Y   L   G  P+  P  S     Y+LA  SLPH+I  +E +LG
Sbjct: 201 CGLSAICRAVSCEQILYYAML---GVNPRFDPASSS----YSLAMHSLPHVINPVESRLG 253

Query: 338 TQITD-NPCIHLVVYVPPCAQAPLRIYRKDGQRAAPLASNVEAFTSAKWGGIVFANPAEA 396
           +      P ++ ++YVP  A +PL I  KDG   AP+A+N  AF S +WGGI+  N    
Sbjct: 254 SSAASLYPVLNFLLYVPELAHSPLYIQDKDG---APVATN--AFHSPRWGGIMVYNVDPK 308

Query: 397 TCVRYMETEQFSDVYIHAQDVMPVLLYQLRKIFDLENNTPLLDATLVPYSTIEPRAWEVD 456
               Y  +E    V +    VM V L QLR +F +          L    +     WE+D
Sbjct: 309 I---YNASELPVRVEVDMAQVMEVFLAQLRLLFGIAQPQVPPKCLLSGPKSEGLMTWELD 365

Query: 457 TFMRTNTIYLVHSATTTLQSLIQLLGGIEYIVINDEVGAAIQTAHRKIVAAKQQLRAGRL 516
             +   ++  + +ATTTL SL QLLG I  IVI D+V + +  A   +  A + L  G L
Sbjct: 366 RLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYRAVAAVQKAAEALALGHL 425

Query: 517 QQAALLAREAYTSAERAFFDPSMLALLYFPDEQKYAIYIPLFLPIMIPVVFSFNTILKYF 576
             A   ++EA TS+ERAFFDPS+L LLYFPD+QK+AIYIPLFLP+ +P++ S   I    
Sbjct: 426 SSAFAASQEAVTSSERAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPILLSLVKIFLET 485

Query: 577 RKRKAT 582
            K    
Sbjct: 486 HKSWKK 491
>ref|NP_187374.2| unknown protein; protein id: At3g07180.1, supported by cDNA:
           gi_17065105 [Arabidopsis thaliana]
 gb|AAL32707.1| Unknown protein [Arabidopsis thaliana]
 gb|AAM91117.1| unknown protein [Arabidopsis thaliana]
          Length = 599

 Score =  444 bits (1132), Expect = e-123
 Identities = 93/346 (26%), Positives = 157/346 (44%), Gaps = 25/346 (7%)

Query: 249 ITVLNPRPELQKVHWNARAAAENYIEPFLSELSGITNFTIKTQWIYQVGLEGAGAQPKQV 308
            ++LN  P      W+ +   E  + P    L+ I N T+++Q +Y        +  K++
Sbjct: 262 FSLLNSNPRDWVYDWDFQRIDEALLAPVTKALAPIANITVESQVLYHTPKSSFSSWDKKL 321

Query: 309 PDDSKLGRHYALAEDSLPHIITSLEKKLGTQI---TDNPCIHLVVYVPPCAQAPLRIYRK 365
                  + Y      LP  + S E  L T       +  +  VVY+P   + PL +   
Sbjct: 322 -------QSYIFRTSDLPFFVNSNEWHLDTSAGASGRSKILQFVVYIPSGKECPLLL--- 371

Query: 366 DGQRAAPLASNVEAFTSAKWGGIVFANPAEATCVRYMETEQFSDVYIHAQD---VMPVLL 422
             Q      S    F S  WGG++  NP    C +  ++E  S   I  QD   ++ + L
Sbjct: 372 --QLPNVEISKTNGFISPMWGGVIVWNP--GNCDK--DSESPSRNTISLQDLEQIVEIFL 425

Query: 423 YQLRKIFDLENN---TPLLDATLVPYSTIEPRAWEVDTFMRTNTIYLVHSATTTLQSLIQ 479
            Q R++F  ++    T  L    +  S      WE+D   R +T + +HS +TTL SL +
Sbjct: 426 GQFRQLFGFKSEAKYTTGLGTYKILTSERGFTEWELDVLSRKHTCFNLHSCSTTLGSLSR 485

Query: 480 LLGGIEYIVINDEVGAAIQTAHRKIVAAKQQLRAGRLQQAALLAREAYTSAERAFFDPSM 539
           L+  +  ++I DE+G  ++ + +    A+     G    +A  +REA + AE AFF PS+
Sbjct: 486 LVRSLPRMIIKDEIGEQVKYSLKAAKLAQSNASLGGYSSSASSSREARSLAENAFFHPSI 545

Query: 540 LALLYFPDEQKYAIYIPLFLPIMIPVVFSFNTILKYFRKRKATTAT 585
           +++ YF  E  +A+Y P FLP++  VV +     K +++ KA   T
Sbjct: 546 MSVSYFSYEHCFAVYSPFFLPVVGHVVLAAVREWKRYKQEKAKYLT 591
 Score = 40.9 bits (95), Expect = 0.096
 Identities = 13/36 (36%), Positives = 22/36 (61%)

Query: 70  VIGVPMWWKTTEVYRVSLPYSEIRALNEDPIKATFR 105
           ++GVP  WK+ E+YR  LP+ +I +L++       R
Sbjct: 42  LLGVPFLWKSVEIYRSPLPFHDIDSLSDQLESTPLR 77
>gb|AAF20228.1|AC012395_15 unknown protein [Arabidopsis thaliana]
          Length = 605

 Score =  398 bits (1014), Expect = e-109
 Identities = 83/315 (26%), Positives = 141/315 (44%), Gaps = 25/315 (7%)

Query: 249 ITVLNPRPELQKVHWNARAAAENYIEPFLSELSGITNFTIKTQWIYQVGLEGAGAQPKQV 308
            ++LN  P      W+ +   E  + P    L+ I N T+++Q +Y        +  K++
Sbjct: 285 FSLLNSNPRDWVYDWDFQRIDEALLAPVTKALAPIANITVESQVLYHTPKSSFSSWDKKL 344

Query: 309 PDDSKLGRHYALAEDSLPHIITSLEKKLGTQI---TDNPCIHLVVYVPPCAQAPLRIYRK 365
                  + Y      LP  + S E  L T       +  +  VVY+P   + PL +   
Sbjct: 345 -------QSYIFRTSDLPFFVNSNEWHLDTSAGASGRSKILQFVVYIPSGKECPLLL--- 394

Query: 366 DGQRAAPLASNVEAFTSAKWGGIVFANPAEATCVRYMETEQFSDVYIHAQD---VMPVLL 422
             Q      S    F S  WGG++  NP    C +  ++E  S   I  QD   ++ + L
Sbjct: 395 --QLPNVEISKTNGFISPMWGGVIVWNP--GNCDK--DSESPSRNTISLQDLEQIVEIFL 448

Query: 423 YQLRKIFDLENN---TPLLDATLVPYSTIEPRAWEVDTFMRTNTIYLVHSATTTLQSLIQ 479
            Q R++F  ++    T  L    +  S      WE+D   R +T + +HS +TTL SL +
Sbjct: 449 GQFRQLFGFKSEAKYTTGLGTYKILTSERGFTEWELDVLSRKHTCFNLHSCSTTLGSLSR 508

Query: 480 LLGGIEYIVINDEVGAAIQTAHRKIVAAKQQLRAGRLQQAALLAREAYTSAERAFFDPSM 539
           L+  +  ++I DE+G  ++ + +    A+     G    +A  +REA + AE AFF PS+
Sbjct: 509 LVRSLPRMIIKDEIGEQVKYSLKAAKLAQSNASLGGYSSSASSSREARSLAENAFFHPSI 568

Query: 540 LALLYFPDEQKYAIY 554
           +++ YF  E  +A+Y
Sbjct: 569 MSVSYFSYEHCFAVY 583
 Score = 38.2 bits (88), Expect = 0.51
 Identities = 13/34 (38%), Positives = 20/34 (58%)

Query: 72  GVPMWWKTTEVYRVSLPYSEIRALNEDPIKATFR 105
           GVP  WK+ E+YR  LP+ +I +L++       R
Sbjct: 67  GVPFLWKSVEIYRSPLPFHDIDSLSDQLESTPLR 100
>ref|NP_010722.1| Glycosyl Phosphatidyl Inositol 17; Gpi17p [Saccharomyces
           cerevisiae]
 sp|Q04080|GP17_YEAST GPI transamidase component GPI17
 pir||S69714 hypothetical protein YDR434w - yeast (Saccharomyces cerevisiae)
 gb|AAB64866.1| Ydr434wp; CAI: 0.14 [Saccharomyces cerevisiae]
          Length = 534

 Score =  393 bits (999), Expect = e-108
 Identities = 116/554 (20%), Positives = 214/554 (37%), Gaps = 58/554 (10%)

Query: 54  AAIHIYATIAFIVIIIVIGVPMWWKTTEVYRVSLPYSEIRALNEDPIKATFRLGLYCQSA 113
           A +  +    F+ I + +GVP+W+K T VYR SLP + I +L  +  +    L +     
Sbjct: 4   ANLRKWVGFCFVAIYLFLGVPLWYKLTTVYRASLPINYIESLQNNKFQ-DIHLVIPVYVK 62

Query: 114 ERREILSFELRKKFEQNFVFELDLQLLSLDADTLSTI---KTPARLEAELQKHYPSTLGH 170
                   ++    +      L+         +L  +   +T  ++E+E  + +  TL  
Sbjct: 63  SDTYRFP-DVHDAIQVQVNHLLN-SQEQRVPWSLQVLPYNETIEQMESEGNQFHVVTLKL 120

Query: 171 FLFIEWPKLDD--DILVTSDRTAFISESASSLKAYQVLSSWLLQEYKLKAILGSRDAQQT 228
             FI +    D  + LV  D  A +S        + V  + +   ++L+    +   +  
Sbjct: 121 DEFIGYSSAYDTKETLVYYDDAAVLSNDLP----FFVAQTLVEHTFQLEW---THLNKTC 173

Query: 229 LMGRQLRLNSAPLQAQYEVLITVLNPRPELQKVHWNARAAAENYIEPFLSELSGITNFTI 288
                    +        + +T+L+       V W       +Y  PF   LS + NFT+
Sbjct: 174 EGVSTNNDVAISYDPNIHLSVTLLSGDGN--PVAWEIEPTLTDYFSPFRKFLSPLVNFTV 231

Query: 289 KTQWIYQVGLEGAGAQPKQVPDDSKLGRHYALAEDSLPHIITSLEKKLGTQITDNPCIHL 348
            +  +Y   L             S  G   ++    L H I   E        ++  ++L
Sbjct: 232 DSSIVYHNDLN----------LHSLNGSCTSVTWFDLSHTIDLSELSSMAYYPEDSALNL 281

Query: 349 VVYVPPCAQAPLRIYRKDGQRAAPLASNVE--AFTSAKWGGIVFANPAEATCVRYMETEQ 406
            +  P  + +P  +   +G R +   + ++  ++   +WG I+         +  M  + 
Sbjct: 282 AIVFPSASSSPDGLAFINGTRISDEITTLDWNSYLVPQWGVII---------INKMPLKP 332

Query: 407 FSDVYIHAQDVMPVLLYQL-RKIFDLENNTPLLDATLVPYSTIEPRAWEVDTFMRTNTIY 465
            S +   ++D +  ++Y+    IF L   T      L PY TI       D+F R   + 
Sbjct: 333 NSVI---SEDYLEPMMYRFATDIFQLLGLTEGSQDLLSPYITI-------DSFKRLTILQ 382

Query: 466 LVHSATTTLQSLIQLLGGIEYIVIN----DEVGAAIQTAHRKIVAAKQQLRAGRL--QQA 519
            +  AT TL SL++L    + + I     D V  A+    + I       + G +    A
Sbjct: 383 NLDKATETLWSLVKLTQQFQGMSIPREVSDNVIEALDLRLQIIDLLNDPGKGGDIVWNNA 442

Query: 520 ALLAREAYTSAERAFFDPSMLALLYFPDEQKYAIYIPLFLPIMIPVVFSFNTILKYFRKR 579
             L+ E     E+AFF+  M+   +FP E   A+Y+PL  PI   + F F  ++K   ++
Sbjct: 443 LHLSNELVKLCEKAFFNGEMVQQNFFPQEHMIAVYLPLLGPISAVMFFGFYNVMKEKNQK 502

Query: 580 KATTATK---AKEE 590
                T+   AKE+
Sbjct: 503 SKKNGTEREVAKEK 516
>ref|NP_594335.1| hypothetical protein [Schizosaccharomyces pombe]
 sp|Q10351|PIGS_SCHPO GPI transamidase component PIG-S homolog
 pir||S67452 hypothetical protein SPAC1F12.09 - fission yeast
           (Schizosaccharomyces pombe)
 emb|CAA93813.1| hypothetical protein [Schizosaccharomyces pombe]
          Length = 554

 Score =  349 bits (888), Expect = 1e-94
 Identities = 94/364 (25%), Positives = 158/364 (42%), Gaps = 42/364 (11%)

Query: 222 SRDAQQTLMGRQLRLNSAPLQAQYEVLITVLNPRPELQKVHWNARAAAENYIEPFLSELS 281
           +R +      R     +     QY VL+++L      + ++W+   A + Y  P + +L+
Sbjct: 217 TRFSSTVEPSRAETKRTVQFSPQYRVLLSLLVGEGNHEPINWDIENAIQKYFNPLIEQLA 276

Query: 282 GITNFTIKTQWIYQVGLEGAGAQPKQVPDDSKLGRHYALAEDSLPHIITSLEKKLG-TQI 340
            +    I+TQ  Y V  E A A  K   DD    +H       LP+++ + EK L  +  
Sbjct: 277 SLAKLNIETQIQYFV--EDAEAYIK---DDKFCTKH-----ADLPNLVNNFEKYLSFSPH 326

Query: 341 TDNPCIHLVVYVPPCAQAPLRIYRKDGQRAAPLASNVEAFTS---AKWGGIVFANPAEAT 397
              P IH V+YVP     PL +  +D        SN+    S    +WG I        T
Sbjct: 327 IREPTIHFVLYVPSPQIQPLWLENED--------SNIIPTNSMLLPQWGSI--------T 370

Query: 398 CVRYMETEQFSDVYIHAQDVMPVLLYQLRKIFDLENNTPLLDATLVPYSTIEPRAWEVDT 457
            + +  TE+     +  +D   V+   L  +  + N+ P+              A   D 
Sbjct: 371 TINFNVTEKKLLHDVDLKDYFRVISRDLLLLLGI-NDVPVSS----------LSATLADR 419

Query: 458 FMRTNTIYLVHSATTTLQSLIQLLGGIEYIVINDEVGAAIQTAHRKIVAAKQQLRAGRLQ 517
            +R         A+ TLQ+L +L+  ++ + +  E+   ++     +  A + L    L 
Sbjct: 420 LLRQRIAESCIEASDTLQNLAKLVHSMQSMAVPKEIQMYVKDTLLSLDMAYKALSQNNLN 479

Query: 518 QAALLAREAYTSAERAFFDPSMLALLYFPDEQKYAIYIPLFLPIMIPVVFSF-NTILKYF 576
           +A   +  A++ ++ A F PSM+  +YFPDE KY IY PLF PI+IP++ SF   +    
Sbjct: 480 EALSYSNNAFSKSQEALFHPSMVTTIYFPDESKYGIYAPLFAPILIPLLISFIKEVKDML 539

Query: 577 RKRK 580
           R+RK
Sbjct: 540 RERK 543
 Score = 39.0 bits (90), Expect = 0.30
 Identities = 16/55 (29%), Positives = 32/55 (58%), Gaps = 1/55 (1%)

Query: 37  ASGEQCEAGSSSAAREDAAIHIYATIAFIVIIIVIGVPMWWKTTEVYRVSLPYSE 91
           +   + E  +    + + ++  YA ++F  +II++ +P+WWKTT   R SLP+ +
Sbjct: 52  SDSHEVERSNFQLNKPEKSLKRYALLSF-YVIILLAIPVWWKTTHYERSSLPFED 105
>dbj|BAB92563.1| P0497A05.6 [Oryza sativa (japonica cultivar-group)]
          Length = 557

 Score =  326 bits (828), Expect = 1e-87
 Identities = 73/300 (24%), Positives = 122/300 (40%), Gaps = 38/300 (12%)

Query: 249 ITVLNPRPELQKVHWNARAAAENYIEPFLSELSGITNFTIKTQWIYQVGLEGAGAQPKQV 308
            ++LN  P      W      +  + P +  L  I N  I++Q +Y              
Sbjct: 277 FSLLNADPSDWVYDWEFENIGQRMLTPVIEALRPIANINIESQVLYHTPKSS------YS 330

Query: 309 PDDSKLGRHYALAEDSLPHIITSLEKKLGTQI---TDNPCIHLVVYVPPCAQAPLRIYRK 365
             D KLG +  L+   +P  + S E  L T I     +  +  VVY+P   + PL +   
Sbjct: 331 YSDDKLGGN-VLSVGDIPFFVNSNEWHLDTSISATGRSKVLQFVVYIPSARECPLYLQLP 389

Query: 366 DGQRAAPLASNVEAFTSAKWGGIVFANPAEAT-------CVRYMETEQFSDVYIHAQDVM 418
           DG       S   AF S  WGG+V  NP   +        +  M +E+         + +
Sbjct: 390 DG-----ELSKTNAFISPMWGGVVIWNPPGCSFGSKPHGALDKMSSEE-------LMETI 437

Query: 419 PVLLYQLRKIFDLENNTPLLDATLVPYSTIEPRA---WEVDTFMRTNTIYLVHSATTTLQ 475
            + + QLR++F L+++        V      P+    WE+D   R +    + S  TTL+
Sbjct: 438 EIFIGQLRQLFGLKSSYHTQSMDGVTKFITSPKGFAQWELDLLYRHHACSNLLSCLTTLE 497

Query: 476 SLIQLLGGIEYIVINDEVGA----AIQTAHRKIVAAKQQLR--AGRLQQAALLAREAYTS 529
           SL  L+  +  +++ DE+G     A  + H  +   K+  R    R + +A LA +A +S
Sbjct: 498 SLSSLVQSLPRMIVMDEIGRQPFFAPVSLHVLLAVMKELKRYMVERRKYSAFLASQATSS 557
 Score = 38.2 bits (88), Expect = 0.55
 Identities = 23/96 (23%), Positives = 42/96 (42%), Gaps = 6/96 (6%)

Query: 2  VSMSAKTPSEETKAQTKASSGDMATDKRRVQQDDDASGEQCEAGSSSAAREDAAIHIYAT 61
          +++ A+   +E+      S G  A+         DA G     G   + R          
Sbjct: 1  MAVLAEIAGDESPPPLPPSEGGEASGAPSTSSPSDADG-----GGKPSPRTSKPGRKRLV 55

Query: 62 I-AFIVIIIVIGVPMWWKTTEVYRVSLPYSEIRALN 96
          + A +++  +IG+P+  K+TE++R  LP   I AL+
Sbjct: 56 LTASVLLSFLIGLPLLLKSTEIHRSPLPSDAIAALS 91
>ref|XP_142436.2| similar to phosphatidylinositol glycan class S [Homo sapiens] [Mus
           musculus]
          Length = 207

 Score =  278 bits (704), Expect = 4e-73
 Identities = 73/215 (33%), Positives = 111/215 (50%), Gaps = 26/215 (12%)

Query: 185 VTSDRTA----FISESASSL---KAYQVLSSWLLQEYKLKAILGSRDAQQTLMGRQLRLN 237
           +  +RTA     I   A ++   +  QV  +  L E  L A L     +      + R  
Sbjct: 5   IGPERTAVVRGLIHREAFNIIGRRIVQVAQAMSLTEDVLAAALADHLPEDKWSSDKRR-- 62

Query: 238 SAPLQAQ--YEVLITVLNPRPELQKVHWNARAAAENYIEPFLSELSGITNFTIKTQWIYQ 295
             PL++   YE+  ++LNP P+   VHW+   A + +++PFL+ LS   NF++ +Q +Y 
Sbjct: 63  --PLKSSLGYEITFSLLNPDPKSHDVHWDIEGAVQRFVQPFLNRLSVAGNFSVDSQILYY 120

Query: 296 VGLEGAGAQPKQVPDDSKLGRHYALAEDSLPHIITSLEKKLGTQITD-NPCIHLVVYVPP 354
             L   G  P+  P  S     Y+LA  SLPH+I  +E +LG+      P +H ++YVP 
Sbjct: 121 AML---GVNPRFDPASSS----YSLAMHSLPHVINPVESRLGSSAASLYPVLHFLLYVPE 173

Query: 355 CAQAPLRIYRKDGQRAAPLASNVEAFTSAKWGGIV 389
            A +PL I  KDG   AP+A+N  AF S +WGGI+
Sbjct: 174 LAHSPLYIQDKDG---APVATN--AFHSPRWGGIM 203
>ref|XP_194600.1| similar to phosphatidylinositol glycan class S [Homo sapiens] [Mus
           musculus]
          Length = 137

 Score =  171 bits (429), Expect = 5e-41
 Identities = 61/132 (46%), Positives = 83/132 (62%)

Query: 451 RAWEVDTFMRTNTIYLVHSATTTLQSLIQLLGGIEYIVINDEVGAAIQTAHRKIVAAKQQ 510
             WE+D  +   ++  + +ATTTL SL QLLG I  IVI D+V + +  A   +  A + 
Sbjct: 1   MTWELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYRAVAAVQKAAKA 60

Query: 511 LRAGRLQQAALLAREAYTSAERAFFDPSMLALLYFPDEQKYAIYIPLFLPIMIPVVFSFN 570
           L  G L  A   ++EA TS+ERAFFDPS+L LLYFPD+QK+AIYIPLFLP+ +P++ S  
Sbjct: 61  LALGHLSSAFAASQEAVTSSERAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPILLSLV 120

Query: 571 TILKYFRKRKAT 582
            I +  RK    
Sbjct: 121 KIFQETRKSWKK 132
>ref|NP_501852.1| Putative endoplasmic reticulum membrane protein, with at least 2
           transmembrane domains, a coiled coil-4 domain, of
           bilaterial origin [Caenorhabditis elegans]
 pir||T24913 hypothetical protein T14G10.7 - Caenorhabditis elegans
 emb|CAA93093.1| Hypothetical protein T14G10.7 [Caenorhabditis elegans]
          Length = 544

 Score =  141 bits (352), Expect = 6e-32
 Identities = 42/124 (33%), Positives = 62/124 (49%), Gaps = 4/124 (3%)

Query: 453 WEVDTFMRTNTIYLVHSATTTLQSLIQLLGGIEYIVINDEVGAAIQTAHRKIVAAKQQLR 512
           WEV       ++     A + +++L QL   I  IVIND+V      A R +      +R
Sbjct: 419 WEVARARLRASVDNAMRAASAVRALDQLAQKISNIVINDDVA---DRATRAVEILNDVVR 475

Query: 513 AGRLQQAALLAREAYTSAERAFFDPSMLALLYFPDEQKYAIYIPLFLPIMIPVVFSFNTI 572
            GR+     L  EA   A+ A  D S+LA+LYFP +Q+ A+ IPL +PI++P+      I
Sbjct: 476 PGRMLDFERLL-EARRLADSANSDHSLLAMLYFPMDQRTAVLIPLAVPIVLPICKLVYEI 534

Query: 573 LKYF 576
            K  
Sbjct: 535 AKIT 538
 Score = 40.5 bits (94), Expect = 0.11
 Identities = 15/54 (27%), Positives = 27/54 (49%)

Query: 51  REDAAIHIYATIAFIVIIIVIGVPMWWKTTEVYRVSLPYSEIRALNEDPIKATF 104
             +    + A+++F++++  IG+PMW  TT  YR + P          PI+  F
Sbjct: 39  HSERPYKLAASLSFVILVFFIGMPMWHFTTSTYRSNFPTFPSNQSITVPIRVIF 92
>ref|XP_220635.1| similar to phosphatidylinositol glycan class S [Homo sapiens]
           [Rattus norvegicus]
          Length = 108

 Score =  126 bits (315), Expect = 1e-27
 Identities = 28/87 (32%), Positives = 43/87 (49%), Gaps = 4/87 (4%)

Query: 53  DAAIHIYATIAFIVIIIVIGVPMWWKTTEVYRVSLPYSEIRALNEDPIKATFRLGLYCQS 112
           +      A + F  + I++G+P+WWKTTE YR  LPYSEI  LN        RL +    
Sbjct: 21  EVVRGKRAALFFAAVAILLGLPLWWKTTETYRAPLPYSEISGLNA----LQLRLMVPVTV 76

Query: 113 AERREILSFELRKKFEQNFVFELDLQL 139
              R+ +  + ++K     V E ++ L
Sbjct: 77  VFTRDSVPLDDQEKLPFTVVHEREIPL 103
CPU time:   136.71 user secs.	    2.84 sys. secs	  139.55 total secs.

  Database: nr
    Posted date:  Jan 18, 2003  1:23 PM
  Number of letters in database: 421,297,171
  Number of sequences in database:  1,315,992
  
Lambda     K      H
   0.311    0.159    0.433 

Gapped
Lambda     K      H
   0.270   0.0556    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 406660635
Number of Sequences: 1315992
Number of extensions: 14880584
Number of successful extensions: 44679
Number of sequences better than 10.0: 31
Number of HSP's better than 10.0 without gapping: 16
Number of HSP's successfully gapped in prelim test: 15
Number of HSP's that attempted gapping in prelim test: 44575
Number of HSP's gapped (non-prelim): 38
length of query: 590
length of database: 421,297,171
effective HSP length: 62
effective length of query: 528
effective length of database: 339,705,667
effective search space: 179364592176
effective search space used: 179364592176
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.0 bits)
S2: 77 (34.2 bits)