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GPI Anchor Biosynthesis Report: NP_035952.1 (PIG-Q family, Mus musculus)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query=
         (581 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!

 Results of PSI-Blast iteration 1

Distribution of 19 Blast Hits on the Query Sequence

Sequences with E-value BETTER than threshold (0.002)




 dbj|BAA84658.1|  (AB008921) mGpi1 [Mus musculus] >gi|15679960|gb|...   990  0.0

 ref|NP_035952.1|  (NM_011822) glycosylphosphatidylinositol 1 homo...   987  0.0

 gb|AAC79574.1|  (AF030178) N-acetylglucosaminyl transferase compo...   979  0.0

 gb|AAK61235.1|AE006464_3  (AE006464) N-acetylglucosamyl transfera...   975  0.0

 ref|NP_004195.1|  (NM_004204) phosphatidylinositol glycan, class ...   973  0.0

 emb|CAB56148.1|  (Z98883) c407A10.1 (GPI1 (N-acetylglucosaminyl t...   970  0.0

 ref|XP_027458.2|  (XM_027458) similar to phosphatidylinositol gly...   861  0.0

 gb|AAH03917.1|AAH03917  (BC003917) Unknown (protein for IMAGE:348...   548  e-155

 dbj|BAB30464.1|  (AK016852) data source:MGD, source key:MGI:13331...   541  e-152

 gb|AAH10094.1|AAH10094  (BC010094) Similar to phosphatidylinosito...   515  e-145

 sp|O14357|GPI1_SCHPO  N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL B...   361  2e-98

 gb|AAC49650.1|  (U77355) Gpi1+ [Schizosaccharomyces pombe]             353  4e-96

 ref|NP_011732.1|  (NC_001139) Participates in synthesis of N-acet...   341  2e-92

 emb|CAC10160.1|  (AJ249657) N-acetylglucosamine transferase [Plas...   308  1e-82

 emb|CAC10056.1|  (AJ298995) GPI1 protein [Drosophila melanogaster]     304  3e-81

 emb|CAC10057.1|  (AJ298996) GPI1 protein [Drosophila virilis]          283  7e-75

 ref|NP_502086.1|  (NM_069685) F01G4.5.p [Caenorhabditis elegans] ...   262  1e-68

 ref|NP_191276.1|  (NM_115577) putative protein [Arabidopsis thali...   238  2e-61

 pir||T45794  hypothetical protein F28O9.10 - Arabidopsis thaliana...   124  4e-27


Alignments


>dbj|BAA84658.1| (AB008921) mGpi1 [Mus musculus]
 gb|AAH14287.1|AAH14287 (BC014287) Similar to glycosylphosphatidylinositol 1 homolog
           (human) [Mus musculus]
          Length = 581

 Score =  990 bits (2532), Expect = 0.0
 Identities = 580/581 (99%), Positives = 580/581 (99%)

Query: 1   MVLKVFFPTCCASADSGLLVGRWVPGQSSAVILAVVHFPFIPIQVKELLAQVQKASQVPV 60
           MVLKVFFPTCCASADSGLLVGRWVPGQSSAVILAVVHFPFIPIQVKELLAQVQKASQVPV
Sbjct: 1   MVLKVFFPTCCASADSGLLVGRWVPGQSSAVILAVVHFPFIPIQVKELLAQVQKASQVPV 60

Query: 61  AVLGTWCHRQQEPQESLGNFLEGLGTIFSHDPWLQLCRERGTRLWSCKATYPQMSNPLDM 120
           AVLGTWCHRQQEPQESLGNFLEGLGTIFSHDPWLQLCRERGTRLWSCKATYPQMSNPLDM
Sbjct: 61  AVLGTWCHRQQEPQESLGNFLEGLGTIFSHDPWLQLCRERGTRLWSCKATYPQMSNPLDM 120

Query: 121 HPEEQVMLIFYDQRKLLLSWLHPPPVLPACQMGDTTASTGGLADIFDTVARSEVLFRNDQ 180
           HPEEQVMLIFYDQRKLLLSWLHPPPVLPACQMGDTTASTGGLADIFDTVARSEVLFRNDQ
Sbjct: 121 HPEEQVMLIFYDQRKLLLSWLHPPPVLPACQMGDTTASTGGLADIFDTVARSEVLFRNDQ 180

Query: 181 FDERPVRLSHWQSEGVEASILVELAKRASGPVCLLLASLLSLISAASACRLWKLWPLSFI 240
           FDERPVRLSHWQSEGVEASILVELAKRASGPVCLLLASLLSLISAASACRLWKLWPLSFI
Sbjct: 181 FDERPVRLSHWQSEGVEASILVELAKRASGPVCLLLASLLSLISAASACRLWKLWPLSFI 240

Query: 241 RSKLSTCEQLHHRLKHLSFIFSTEKAQNPMQLMRKANMLVSVLLDVALGLLLLSWLHSNN 300
           RSKLSTCEQLHHRLKHLSFIFSTEKAQNPMQLMRKANMLVSVLLDVALGLLLLSWLHSNN
Sbjct: 241 RSKLSTCEQLHHRLKHLSFIFSTEKAQNPMQLMRKANMLVSVLLDVALGLLLLSWLHSNN 300

Query: 301 RIGQLANALVPVADRVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIH 360
           RIGQLANALVPVADRVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIH
Sbjct: 301 RIGQLANALVPVADRVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIH 360

Query: 361 LMSPFIEHILWHVGLSACLGLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSL 420
           LMSPFIEHILWHVGLSACLGLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSL
Sbjct: 361 LMSPFIEHILWHVGLSACLGLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSL 420

Query: 421 WRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILVFLLPTTALYCLVFTLLRLLVITV 480
           WRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILVFLLPTTALY LVFTLLRLLVITV
Sbjct: 421 WRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILVFLLPTTALYYLVFTLLRLLVITV 480

Query: 481 QGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLEKEAGRPLRLLMQINPLSYNHV 540
           QGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLEKEAGRPLRLLMQINPLSYNHV
Sbjct: 481 QGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLEKEAGRPLRLLMQINPLSYNHV 540

Query: 541 MHIYRLPRCGCHPKHSWGTLCRKLVFGELIYPWRQREDKQD 581
           MHIYRLPRCGCHPKHSWGTLCRKLVFGELIYPWRQREDKQD
Sbjct: 541 MHIYRLPRCGCHPKHSWGTLCRKLVFGELIYPWRQREDKQD 581





>ref|NP_035952.1| (NM_011822) glycosylphosphatidylinositol 1 homolog (human);
           glycosylphosphatidylinositol homolog (S. cerevisiae)
           [Mus musculus]
 dbj|BAA23615.1| (AB008895) mGpi1p [Mus musculus]
          Length = 581

 Score =  987 bits (2526), Expect = 0.0
 Identities = 581/581 (100%), Positives = 581/581 (100%)

Query: 1   MVLKVFFPTCCASADSGLLVGRWVPGQSSAVILAVVHFPFIPIQVKELLAQVQKASQVPV 60
           MVLKVFFPTCCASADSGLLVGRWVPGQSSAVILAVVHFPFIPIQVKELLAQVQKASQVPV
Sbjct: 1   MVLKVFFPTCCASADSGLLVGRWVPGQSSAVILAVVHFPFIPIQVKELLAQVQKASQVPV 60

Query: 61  AVLGTWCHRQQEPQESLGNFLEGLGTIFSHDPWLQLCRERGTRLWSCKATYPQMSNPLDM 120
           AVLGTWCHRQQEPQESLGNFLEGLGTIFSHDPWLQLCRERGTRLWSCKATYPQMSNPLDM
Sbjct: 61  AVLGTWCHRQQEPQESLGNFLEGLGTIFSHDPWLQLCRERGTRLWSCKATYPQMSNPLDM 120

Query: 121 HPEEQVMLIFYDQRKLLLSWLHPPPVLPACQMGDTTASTGGLADIFDTVARSEVLFRNDQ 180
           HPEEQVMLIFYDQRKLLLSWLHPPPVLPACQMGDTTASTGGLADIFDTVARSEVLFRNDQ
Sbjct: 121 HPEEQVMLIFYDQRKLLLSWLHPPPVLPACQMGDTTASTGGLADIFDTVARSEVLFRNDQ 180

Query: 181 FDERPVRLSHWQSEGVEASILVELAKRASGPVCLLLASLLSLISAASACRLWKLWPLSFI 240
           FDERPVRLSHWQSEGVEASILVELAKRASGPVCLLLASLLSLISAASACRLWKLWPLSFI
Sbjct: 181 FDERPVRLSHWQSEGVEASILVELAKRASGPVCLLLASLLSLISAASACRLWKLWPLSFI 240

Query: 241 RSKLSTCEQLHHRLKHLSFIFSTEKAQNPMQLMRKANMLVSVLLDVALGLLLLSWLHSNN 300
           RSKLSTCEQLHHRLKHLSFIFSTEKAQNPMQLMRKANMLVSVLLDVALGLLLLSWLHSNN
Sbjct: 241 RSKLSTCEQLHHRLKHLSFIFSTEKAQNPMQLMRKANMLVSVLLDVALGLLLLSWLHSNN 300

Query: 301 RIGQLANALVPVADRVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIH 360
           RIGQLANALVPVADRVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIH
Sbjct: 301 RIGQLANALVPVADRVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIH 360

Query: 361 LMSPFIEHILWHVGLSACLGLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSL 420
           LMSPFIEHILWHVGLSACLGLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSL
Sbjct: 361 LMSPFIEHILWHVGLSACLGLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSL 420

Query: 421 WRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILVFLLPTTALYCLVFTLLRLLVITV 480
           WRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILVFLLPTTALYCLVFTLLRLLVITV
Sbjct: 421 WRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILVFLLPTTALYCLVFTLLRLLVITV 480

Query: 481 QGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLEKEAGRPLRLLMQINPLSYNHV 540
           QGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLEKEAGRPLRLLMQINPLSYNHV
Sbjct: 481 QGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLEKEAGRPLRLLMQINPLSYNHV 540

Query: 541 MHIYRLPRCGCHPKHSWGTLCRKLVFGELIYPWRQREDKQD 581
           MHIYRLPRCGCHPKHSWGTLCRKLVFGELIYPWRQREDKQD
Sbjct: 541 MHIYRLPRCGCHPKHSWGTLCRKLVFGELIYPWRQREDKQD 581





>gb|AAC79574.1| (AF030178) N-acetylglucosaminyl transferase component Gpi1 [Mus
           musculus]
          Length = 581

 Score =  979 bits (2505), Expect = 0.0
 Identities = 549/581 (94%), Positives = 561/581 (96%)

Query: 1   MVLKVFFPTCCASADSGLLVGRWVPGQSSAVILAVVHFPFIPIQVKELLAQVQKASQVPV 60
           MVLK FFPTCCASADSGLLVGRWVPGQ+SAVILAVVHFPFIPIQVKELLAQVQKASQV V
Sbjct: 1   MVLKAFFPTCCASADSGLLVGRWVPGQNSAVILAVVHFPFIPIQVKELLAQVQKASQVQV 60

Query: 61  AVLGTWCHRQQEPQESLGNFLEGLGTIFSHDPWLQLCRERGTRLWSCKATYPQMSNPLDM 120
            VLGTWCHRQQEP+ESLG FLEGLG IFSH+PWLQLCRE+GTR WSCKATY QMS+ LD 
Sbjct: 61  TVLGTWCHRQQEPEESLGKFLEGLGAIFSHNPWLQLCREKGTRFWSCKATYHQMSSTLDT 120

Query: 121 HPEEQVMLIFYDQRKLLLSWLHPPPVLPACQMGDTTASTGGLADIFDTVARSEVLFRNDQ 180
             E+QVMLIFYDQRKLLLSWLHPPPVLP CQ+GD+TASTGGLADIFDTVARSEVLFRNDQ
Sbjct: 121 PTEDQVMLIFYDQRKLLLSWLHPPPVLPDCQIGDSTASTGGLADIFDTVARSEVLFRNDQ 180

Query: 181 FDERPVRLSHWQSEGVEASILVELAKRASGPVCLLLASLLSLISAASACRLWKLWPLSFI 240
           FDE PVRLSHWQSEGVEASILVELAKRASGPVCLLLASLLSLISAASACRLWKL PL+FI
Sbjct: 181 FDEGPVRLSHWQSEGVEASILVELAKRASGPVCLLLASLLSLISAASACRLWKLGPLAFI 240

Query: 241 RSKLSTCEQLHHRLKHLSFIFSTEKAQNPMQLMRKANMLVSVLLDVALGLLLLSWLHSNN 300
           RSKLSTCEQL HRLKHLSFIFSTEKAQ+PMQLMRKANMLVSVLLDVALGLLLLSWLHSNN
Sbjct: 241 RSKLSTCEQLQHRLKHLSFIFSTEKAQSPMQLMRKANMLVSVLLDVALGLLLLSWLHSNN 300

Query: 301 RIGQLANALVPVADRVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIH 360
           RIGQLANALVPVADRVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIH
Sbjct: 301 RIGQLANALVPVADRVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIH 360

Query: 361 LMSPFIEHILWHVGLSACLGLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSL 420
           LMSPFIEHILWHVGLSACLGLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSL
Sbjct: 361 LMSPFIEHILWHVGLSACLGLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSL 420

Query: 421 WRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILVFLLPTTALYCLVFTLLRLLVITV 480
           WRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILVFLLPTTALY LVFTLLRLLVITV
Sbjct: 421 WRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILVFLLPTTALYYLVFTLLRLLVITV 480

Query: 481 QGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLEKEAGRPLRLLMQINPLSYNHV 540
           QGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLEKEAGRPLRLLMQINPL Y+HV
Sbjct: 481 QGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLEKEAGRPLRLLMQINPLPYSHV 540

Query: 541 MHIYRLPRCGCHPKHSWGTLCRKLVFGELIYPWRQREDKQD 581
           +H YRLP CGCHPKHSWGTLCRKL FGELIYPWRQREDKQD
Sbjct: 541 VHTYRLPSCGCHPKHSWGTLCRKLFFGELIYPWRQREDKQD 581





>gb|AAK61235.1|AE006464_3 (AE006464) N-acetylglucosamyl transferase component GPI1 [Homo
           sapiens]
          Length = 581

 Score =  975 bits (2495), Expect = 0.0
 Identities = 485/581 (83%), Positives = 523/581 (89%)

Query: 1   MVLKVFFPTCCASADSGLLVGRWVPGQSSAVILAVVHFPFIPIQVKELLAQVQKASQVPV 60
           MVLK FFPTCC S DSGLLVGRWVP QSSAV+LAV+HFPFIPIQVK+LLAQV++ASQV V
Sbjct: 1   MVLKAFFPTCCVSTDSGLLVGRWVPEQSSAVVLAVLHFPFIPIQVKQLLAQVRQASQVGV 60

Query: 61  AVLGTWCHRQQEPQESLGNFLEGLGTIFSHDPWLQLCRERGTRLWSCKATYPQMSNPLDM 120
           AVLGTWCH +QEP+ESLG FLE LG +F H+PWL+LCRERG   WSC+AT+ Q       
Sbjct: 61  AVLGTWCHCRQEPEESLGRFLESLGAVFPHEPWLRLCRERGGTFWSCEATHRQAPTAPGA 120

Query: 121 HPEEQVMLIFYDQRKLLLSWLHPPPVLPACQMGDTTASTGGLADIFDTVARSEVLFRNDQ 180
             E+QVMLIFYDQR++LLS LH P VLP  Q G TTASTGGLA +FDTVARSEVLFR+D+
Sbjct: 121 PGEDQVMLIFYDQRQVLLSQLHLPTVLPDRQAGATTASTGGLAAVFDTVARSEVLFRSDR 180

Query: 181 FDERPVRLSHWQSEGVEASILVELAKRASGPVCLLLASLLSLISAASACRLWKLWPLSFI 240
           FDE PVRLSHWQSEGVEASIL ELA+RASGP+CLLLASLLSL+SA SACR++KLWPLSF+
Sbjct: 181 FDEGPVRLSHWQSEGVEASILAELARRASGPICLLLASLLSLVSAVSACRVFKLWPLSFL 240

Query: 241 RSKLSTCEQLHHRLKHLSFIFSTEKAQNPMQLMRKANMLVSVLLDVALGLLLLSWLHSNN 300
            SKLSTCEQL HRL+HL+ IFST KA+NP QLMRKAN + SVLLDVALGL+LLSWLH  +
Sbjct: 241 GSKLSTCEQLRHRLEHLTLIFSTRKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRS 300

Query: 301 RIGQLANALVPVADRVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIH 360
           RIG LA+ALVPVAD VAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIH
Sbjct: 301 RIGHLADALVPVADHVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIH 360

Query: 361 LMSPFIEHILWHVGLSACLGLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSL 420
           LMSPF+EHILWHVGLSACLGLTVALS+ SDIIALLTFHIYCFYVYGARLYCLKI+GLSSL
Sbjct: 361 LMSPFVEHILWHVGLSACLGLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSL 420

Query: 421 WRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILVFLLPTTALYCLVFTLLRLLVITV 480
           WRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTIL+FLLPTTALY LVFTLLRLLV+ V
Sbjct: 421 WRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAV 480

Query: 481 QGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLEKEAGRPLRLLMQINPLSYNHV 540
           QGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVL  EAGRPLRLLMQINPL Y+ V
Sbjct: 481 QGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLRHEAGRPLRLLMQINPLPYSRV 540

Query: 541 MHIYRLPRCGCHPKHSWGTLCRKLVFGELIYPWRQREDKQD 581
           +H YRLP CGCHPKHSWG LCRKL  GELIYPWRQR DKQD
Sbjct: 541 VHTYRLPSCGCHPKHSWGALCRKLFLGELIYPWRQRGDKQD 581





>ref|NP_004195.1| (NM_004204) phosphatidylinositol glycan, class Q;
           N-acetylglucosaminyl transferase component Gpi1 [Homo
           sapiens]
 gb|AAC32661.1| (AF030177) N-acetylglucosaminyl transferase component Gpi1 [Homo
           sapiens]
 dbj|BAA24948.1| (AB003723) GPI1 [Homo sapiens]
          Length = 581

 Score =  973 bits (2490), Expect = 0.0
 Identities = 485/581 (83%), Positives = 523/581 (89%)

Query: 1   MVLKVFFPTCCASADSGLLVGRWVPGQSSAVILAVVHFPFIPIQVKELLAQVQKASQVPV 60
           MVLK FFPTCC SADSGLLVGRWVP QSSAV+LAV+HFPFIPIQVK+LLAQV++ASQV V
Sbjct: 1   MVLKAFFPTCCVSADSGLLVGRWVPEQSSAVVLAVLHFPFIPIQVKQLLAQVRQASQVGV 60

Query: 61  AVLGTWCHRQQEPQESLGNFLEGLGTIFSHDPWLQLCRERGTRLWSCKATYPQMSNPLDM 120
           AVLGTWCH +QEP+ESLG FLE LG +F H+PWL+LCRERG   WSC+AT+ Q       
Sbjct: 61  AVLGTWCHCRQEPEESLGRFLESLGAVFPHEPWLRLCRERGGTFWSCEATHRQAPTAPGA 120

Query: 121 HPEEQVMLIFYDQRKLLLSWLHPPPVLPACQMGDTTASTGGLADIFDTVARSEVLFRNDQ 180
             E+QVMLIFYDQR++LLS LH P VLP  Q G TTASTGGLA +FDTVARSEVLFR+D+
Sbjct: 121 PGEDQVMLIFYDQRQVLLSQLHLPTVLPDRQAGATTASTGGLAAVFDTVARSEVLFRSDR 180

Query: 181 FDERPVRLSHWQSEGVEASILVELAKRASGPVCLLLASLLSLISAASACRLWKLWPLSFI 240
           FDE PVRLSHWQSEGVEASIL ELA+RASGP+CLLLASLLSL+SA SACR++KLWPLSF+
Sbjct: 181 FDEGPVRLSHWQSEGVEASILAELARRASGPICLLLASLLSLVSAVSACRVFKLWPLSFL 240

Query: 241 RSKLSTCEQLHHRLKHLSFIFSTEKAQNPMQLMRKANMLVSVLLDVALGLLLLSWLHSNN 300
            SKLSTCEQL HRL+HL+ IFST KA+NP QLMRKAN + SVLLDVALGL+LLSWLH  +
Sbjct: 241 GSKLSTCEQLRHRLEHLTLIFSTRKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRS 300

Query: 301 RIGQLANALVPVADRVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIH 360
           RIG LA+ALVPVAD VAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIH
Sbjct: 301 RIGHLADALVPVADHVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIH 360

Query: 361 LMSPFIEHILWHVGLSACLGLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSL 420
           LMSPF+EHILWHVGLSACLGLTVALS+ SDIIALLTFHIYCFYVYGARLYCLKI+GLSSL
Sbjct: 361 LMSPFVEHILWHVGLSACLGLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSL 420

Query: 421 WRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILVFLLPTTALYCLVFTLLRLLVITV 480
           WRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTIL+FLLPTTALY LVFTLLRLLV+ V
Sbjct: 421 WRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAV 480

Query: 481 QGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLEKEAGRPLRLLMQINPLSYNHV 540
           QGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVL  EA RPLRLLMQINPL Y+ V
Sbjct: 481 QGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLRHEASRPLRLLMQINPLPYSRV 540

Query: 541 MHIYRLPRCGCHPKHSWGTLCRKLVFGELIYPWRQREDKQD 581
           +H YRLP CGCHPKHSWG LCRKL  GELIYPWRQR DKQD
Sbjct: 541 VHTYRLPSCGCHPKHSWGALCRKLFLGELIYPWRQRGDKQD 581





>emb|CAB56148.1| (Z98883) c407A10.1 (GPI1 (N-acetylglucosaminyl transferase
           component)) [Homo sapiens]
          Length = 583

 Score =  970 bits (2482), Expect = 0.0
 Identities = 485/583 (83%), Positives = 523/583 (89%), Gaps = 2/583 (0%)

Query: 1   MVLKVFFPTCCASADSGLLVGRWVPGQSSAVILAVVHFPFIPIQVKELLAQVQKASQVPV 60
           MVLK FFPTCC S DSGLLVGRWVP QSSAV+LAV+HFPFIPIQVK+LLAQV++ASQV V
Sbjct: 1   MVLKAFFPTCCVSTDSGLLVGRWVPEQSSAVVLAVLHFPFIPIQVKQLLAQVRQASQVGV 60

Query: 61  AVLGTWCHRQQEPQESLGNFLEGLGTIFSHDPWLQLCRERGTRLWSCKATYPQMSNPLDM 120
           AVLGTWCH +QEP+ESLG FLE LG +F H+PWL+LCRERG   WSC+AT+ Q       
Sbjct: 61  AVLGTWCHCRQEPEESLGRFLESLGAVFPHEPWLRLCRERGGTFWSCEATHRQAPTAPGA 120

Query: 121 HPEEQVMLIFYDQRKLLLSWLHPPPVLPACQMGDTTASTGGLADIFDTVARSEVLFRNDQ 180
             E+QVMLIFYDQR++LLS LH P VLP  Q G TTASTGGLA +FDTVARSEVLFR+D+
Sbjct: 121 PGEDQVMLIFYDQRQVLLSQLHLPTVLPDRQAGATTASTGGLAAVFDTVARSEVLFRSDR 180

Query: 181 FDERPVRLSHWQSEGVEASILVELAKRASGPVCLLLASLLSLISAASAC--RLWKLWPLS 238
           FDE PVRLSHWQSEGVEASIL ELA+RASGP+CLLLASLLSL+SA SAC  R++KLWPLS
Sbjct: 181 FDEGPVRLSHWQSEGVEASILAELARRASGPICLLLASLLSLVSAVSACRRRVFKLWPLS 240

Query: 239 FIRSKLSTCEQLHHRLKHLSFIFSTEKAQNPMQLMRKANMLVSVLLDVALGLLLLSWLHS 298
           F+ SKLSTCEQL HRL+HL+ IFST KA+NP QLMRKAN + SVLLDVALGL+LLSWLH 
Sbjct: 241 FLGSKLSTCEQLRHRLEHLTLIFSTRKAENPAQLMRKANTVASVLLDVALGLMLLSWLHG 300

Query: 299 NNRIGQLANALVPVADRVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISY 358
            +RIG LA+ALVPVAD VAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISY
Sbjct: 301 RSRIGHLADALVPVADHVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISY 360

Query: 359 IHLMSPFIEHILWHVGLSACLGLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLS 418
           IHLMSPF+EHILWHVGLSACLGLTVALS+ SDIIALLTFHIYCFYVYGARLYCLKI+GLS
Sbjct: 361 IHLMSPFVEHILWHVGLSACLGLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLS 420

Query: 419 SLWRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILVFLLPTTALYCLVFTLLRLLVI 478
           SLWRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTIL+FLLPTTALY LVFTLLRLLV+
Sbjct: 421 SLWRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVV 480

Query: 479 TVQGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLEKEAGRPLRLLMQINPLSYN 538
            VQGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVL  EAGRPLRLLMQINPL Y+
Sbjct: 481 AVQGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLRHEAGRPLRLLMQINPLPYS 540

Query: 539 HVMHIYRLPRCGCHPKHSWGTLCRKLVFGELIYPWRQREDKQD 581
            V+H YRLP CGCHPKHSWG LCRKL  GELIYPWRQR DKQD
Sbjct: 541 RVVHTYRLPSCGCHPKHSWGALCRKLFLGELIYPWRQRGDKQD 583





>ref|XP_027458.2| (XM_027458) similar to phosphatidylinositol glycan, class Q [Homo
           sapiens]
 gb|AAH06377.1|AAH06377 (BC006377) Similar to phosphatidylinositol glycan, class Q [Homo
           sapiens]
          Length = 760

 Score =  861 bits (2201), Expect = 0.0
 Identities = 426/510 (83%), Positives = 462/510 (90%)

Query: 1   MVLKVFFPTCCASADSGLLVGRWVPGQSSAVILAVVHFPFIPIQVKELLAQVQKASQVPV 60
           MVLK FFPTCC S DSGLLVGRWVP QSSAV+LAV+HFPFIPIQVK+LLAQV++ASQV V
Sbjct: 1   MVLKAFFPTCCVSTDSGLLVGRWVPEQSSAVVLAVLHFPFIPIQVKQLLAQVRQASQVGV 60

Query: 61  AVLGTWCHRQQEPQESLGNFLEGLGTIFSHDPWLQLCRERGTRLWSCKATYPQMSNPLDM 120
           AVLGTWCH +QEP+ESLG FLE LG +F H+PWL+LCRERG   WSC+AT+ Q       
Sbjct: 61  AVLGTWCHCRQEPEESLGRFLESLGAVFPHEPWLRLCRERGGTFWSCEATHRQAPTAPGA 120

Query: 121 HPEEQVMLIFYDQRKLLLSWLHPPPVLPACQMGDTTASTGGLADIFDTVARSEVLFRNDQ 180
             E+QVMLIFYDQR++LLS LH P VLP  Q G TTASTGGLA +FDTVARSEVLFR+D+
Sbjct: 121 PGEDQVMLIFYDQRQVLLSQLHLPTVLPDRQAGATTASTGGLAAVFDTVARSEVLFRSDR 180

Query: 181 FDERPVRLSHWQSEGVEASILVELAKRASGPVCLLLASLLSLISAASACRLWKLWPLSFI 240
           FDE PVRLSHWQSEGVEASIL ELA+RASGP+CLLLASLLSL+SA SACR++KLWPLSF+
Sbjct: 181 FDEGPVRLSHWQSEGVEASILAELARRASGPICLLLASLLSLVSAVSACRVFKLWPLSFL 240

Query: 241 RSKLSTCEQLHHRLKHLSFIFSTEKAQNPMQLMRKANMLVSVLLDVALGLLLLSWLHSNN 300
            SKLSTCEQL HRL+HL+ IFST KA+NP QLMRKAN + SVLLDVALGL+LLSWLH  +
Sbjct: 241 GSKLSTCEQLRHRLEHLTLIFSTRKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRS 300

Query: 301 RIGQLANALVPVADRVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIH 360
           RIG LA+ALVPVAD VAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIH
Sbjct: 301 RIGHLADALVPVADHVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIH 360

Query: 361 LMSPFIEHILWHVGLSACLGLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSL 420
           LMSPF+EHILWHVGLSACLGLTVALS+ SDIIALLTFHIYCFYVYGARLYCLKI+GLSSL
Sbjct: 361 LMSPFVEHILWHVGLSACLGLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSL 420

Query: 421 WRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILVFLLPTTALYCLVFTLLRLLVITV 480
           WRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTIL+FLLPTTALY LVFTLLRLLV+ V
Sbjct: 421 WRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAV 480

Query: 481 QGLIHLLVDLINSLPLYSLGLRLCRPYRLA 510
           QGLIHLLVDLINSLPLYSLGLRLCRPYRLA
Sbjct: 481 QGLIHLLVDLINSLPLYSLGLRLCRPYRLA 510





>gb|AAH03917.1|AAH03917 (BC003917) Unknown (protein for IMAGE:3485029) [Mus musculus]
          Length = 339

 Score =  548 bits (1398), Expect = e-155
 Identities = 338/339 (99%), Positives = 338/339 (99%)

Query: 243 KLSTCEQLHHRLKHLSFIFSTEKAQNPMQLMRKANMLVSVLLDVALGLLLLSWLHSNNRI 302
           KLSTCEQLHHRLKHLSFIFSTEKAQNPMQLMRKANMLVSVLLDVALGLLLLSWLHSNNRI
Sbjct: 1   KLSTCEQLHHRLKHLSFIFSTEKAQNPMQLMRKANMLVSVLLDVALGLLLLSWLHSNNRI 60

Query: 303 GQLANALVPVADRVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLM 362
           GQLANALVPVADRVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLM
Sbjct: 61  GQLANALVPVADRVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLM 120

Query: 363 SPFIEHILWHVGLSACLGLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSLWR 422
           SPFIEHILWHVGLSACLGLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSLWR
Sbjct: 121 SPFIEHILWHVGLSACLGLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSLWR 180

Query: 423 LFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILVFLLPTTALYCLVFTLLRLLVITVQG 482
           LFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILVFLLPTTALY LVFTLLRLLVITVQG
Sbjct: 181 LFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILVFLLPTTALYYLVFTLLRLLVITVQG 240

Query: 483 LIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLEKEAGRPLRLLMQINPLSYNHVMH 542
           LIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLEKEAGRPLRLLMQINPLSYNHVMH
Sbjct: 241 LIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLEKEAGRPLRLLMQINPLSYNHVMH 300

Query: 543 IYRLPRCGCHPKHSWGTLCRKLVFGELIYPWRQREDKQD 581
           IYRLPRCGCHPKHSWGTLCRKLVFGELIYPWRQREDKQD
Sbjct: 301 IYRLPRCGCHPKHSWGTLCRKLVFGELIYPWRQREDKQD 339





>dbj|BAB30464.1| (AK016852) data source:MGD, source key:MGI:1333114,
           evidence:ISS~phosphatidylinositol glycan, class
           Q~putative [Mus musculus]
          Length = 378

 Score =  541 bits (1380), Expect = e-152
 Identities = 314/314 (100%), Positives = 314/314 (100%)

Query: 1   MVLKVFFPTCCASADSGLLVGRWVPGQSSAVILAVVHFPFIPIQVKELLAQVQKASQVPV 60
           MVLKVFFPTCCASADSGLLVGRWVPGQSSAVILAVVHFPFIPIQVKELLAQVQKASQVPV
Sbjct: 1   MVLKVFFPTCCASADSGLLVGRWVPGQSSAVILAVVHFPFIPIQVKELLAQVQKASQVPV 60

Query: 61  AVLGTWCHRQQEPQESLGNFLEGLGTIFSHDPWLQLCRERGTRLWSCKATYPQMSNPLDM 120
           AVLGTWCHRQQEPQESLGNFLEGLGTIFSHDPWLQLCRERGTRLWSCKATYPQMSNPLDM
Sbjct: 61  AVLGTWCHRQQEPQESLGNFLEGLGTIFSHDPWLQLCRERGTRLWSCKATYPQMSNPLDM 120

Query: 121 HPEEQVMLIFYDQRKLLLSWLHPPPVLPACQMGDTTASTGGLADIFDTVARSEVLFRNDQ 180
           HPEEQVMLIFYDQRKLLLSWLHPPPVLPACQMGDTTASTGGLADIFDTVARSEVLFRNDQ
Sbjct: 121 HPEEQVMLIFYDQRKLLLSWLHPPPVLPACQMGDTTASTGGLADIFDTVARSEVLFRNDQ 180

Query: 181 FDERPVRLSHWQSEGVEASILVELAKRASGPVCLLLASLLSLISAASACRLWKLWPLSFI 240
           FDERPVRLSHWQSEGVEASILVELAKRASGPVCLLLASLLSLISAASACRLWKLWPLSFI
Sbjct: 181 FDERPVRLSHWQSEGVEASILVELAKRASGPVCLLLASLLSLISAASACRLWKLWPLSFI 240

Query: 241 RSKLSTCEQLHHRLKHLSFIFSTEKAQNPMQLMRKANMLVSVLLDVALGLLLLSWLHSNN 300
           RSKLSTCEQLHHRLKHLSFIFSTEKAQNPMQLMRKANMLVSVLLDVALGLLLLSWLHSNN
Sbjct: 241 RSKLSTCEQLHHRLKHLSFIFSTEKAQNPMQLMRKANMLVSVLLDVALGLLLLSWLHSNN 300

Query: 301 RIGQLANALVPVAD 314
           RIGQLANALVPVAD
Sbjct: 301 RIGQLANALVPVAD 314





>gb|AAH10094.1|AAH10094 (BC010094) Similar to phosphatidylinositol glycan, class Q [Homo
           sapiens]
          Length = 299

 Score =  515 bits (1313), Expect = e-145
 Identities = 212/281 (75%), Positives = 239/281 (84%)

Query: 1   MVLKVFFPTCCASADSGLLVGRWVPGQSSAVILAVVHFPFIPIQVKELLAQVQKASQVPV 60
           MVLK FFPTCC SADSGLLVGRWVP QSSAV+LAV+HFPFIPIQVK+LLAQV++ASQV V
Sbjct: 1   MVLKAFFPTCCVSADSGLLVGRWVPEQSSAVVLAVLHFPFIPIQVKQLLAQVRQASQVGV 60

Query: 61  AVLGTWCHRQQEPQESLGNFLEGLGTIFSHDPWLQLCRERGTRLWSCKATYPQMSNPLDM 120
           AVLGTWCH +QEP+ESLG FLE LG +F H+PWL+LCRERG   WSC+AT+ Q       
Sbjct: 61  AVLGTWCHCRQEPEESLGRFLESLGAVFPHEPWLRLCRERGGTFWSCEATHRQAPTAPGA 120

Query: 121 HPEEQVMLIFYDQRKLLLSWLHPPPVLPACQMGDTTASTGGLADIFDTVARSEVLFRNDQ 180
             E+QVMLIFYDQR++LLS LH P VLP  Q G TTASTGGLA +FDTVARSEVLFR+D+
Sbjct: 121 PGEDQVMLIFYDQRQVLLSQLHLPTVLPDRQAGATTASTGGLAAVFDTVARSEVLFRSDR 180

Query: 181 FDERPVRLSHWQSEGVEASILVELAKRASGPVCLLLASLLSLISAASACRLWKLWPLSFI 240
           FDE PVRLSHWQSEGVEASIL ELA+RASGP+CLLLASLLSL+SA SACR++KLWPLSF+
Sbjct: 181 FDEGPVRLSHWQSEGVEASILAELARRASGPICLLLASLLSLVSAVSACRVFKLWPLSFL 240

Query: 241 RSKLSTCEQLHHRLKHLSFIFSTEKAQNPMQLMRKANMLVS 281
            SKLSTCEQL HRL+HL+ IFST KA+NP QLMR    LVS
Sbjct: 241 GSKLSTCEQLRHRLEHLTLIFSTRKAENPAQLMRCGPALVS 281





>sp|O14357|GPI1_SCHPO N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN GPI1
 pir||T40185 n-acetylglucosaminyl-phosphatidylinositol biosynthetic protein gpi1
           - fission yeast (Schizosaccharomyces pombe)
 emb|CAB10806.1| (Z97992) n-acetylglucosaminyl-phosphatidylinositol biosynthetic
           protein gpi1. [Schizosaccharomyces pombe]
          Length = 653

 Score =  361 bits (917), Expect = 2e-98
 Identities = 92/320 (28%), Positives = 168/320 (51%), Gaps = 17/320 (5%)

Query: 259 FIFSTEKA-QNPMQLMRKANMLVSVLLDVALGLLLLSWLHSNNRIGQLANALVPVADRVA 317
           F  S   A ++  + +R  N L  V  D+  G+ + S++  N  +      +V + + + 
Sbjct: 298 FRKSKRVAIEDYKEYIRFYNNLWLVANDMIFGITMSSFILENLHL------VVKLIENIT 351

Query: 318 EE-----LQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFIEHILWH 372
            E     ++ ++ WL+  PAGLK+N  + + + +  ++ I +W +++    P+   ++  
Sbjct: 352 FEYAIKNVRSMVIWLVDTPAGLKLNNDICKFIMKLSVWVIDVWSNFLLHCLPWTPFLVQV 411

Query: 373 VGLSACLGLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSLWRLFRGKKWNVL 432
           V +S   G ++ +++ SD ++++T HI+  Y+  +RLY  ++  + SL +LFRGKK NVL
Sbjct: 412 VAISGFGGASLMIALISDFLSVMTIHIHLLYLASSRLYNWQLRVIYSLLQLFRGKKRNVL 471

Query: 433 RQRVDSCSYDLDQLFIGTLLFTILVFLLPTTALYCLVFTLLRLLVITVQGLIHLLVDLIN 492
           R R+DS  YDLDQL +GT+LFT+L+F LPT  ++   F L R+ V+T   +   ++  +N
Sbjct: 472 RNRIDSYEYDLDQLLLGTILFTVLIFFLPTIYVFYAAFALTRVSVMTCLAICETMLAFLN 531

Query: 493 SLPLYSLGLRLCRPYRLAAGVKFRV-----LEKEAGRPLRLLMQINPLSYNHVMHIYRLP 547
             PL+   LR+  PYR+ +G+ F +     L+++    L L     P+S   +   YR  
Sbjct: 532 HFPLFVTMLRIKDPYRIPSGLNFEIVSFEPLKQDGFATLYLNCNSKPMSLGSMFEHYRKL 591

Query: 548 RCGCHPKHSWGTLCRKLVFG 567
                  +   T    L+ G
Sbjct: 592 ARRLISHYLSKTTLISLLVG 611





>gb|AAC49650.1| (U77355) Gpi1+ [Schizosaccharomyces pombe]
          Length = 517

 Score =  353 bits (898), Expect = 4e-96
 Identities = 91/320 (28%), Positives = 168/320 (52%), Gaps = 17/320 (5%)

Query: 259 FIFSTEKA-QNPMQLMRKANMLVSVLLDVALGLLLLSWLHSNNRIGQLANALVPVADRVA 317
           F  S   A ++  + +R  N L  V  D+  G+ + S++  N  +      +V + + + 
Sbjct: 162 FRKSKRVAIEDYKEYIRFYNNLWLVANDMIFGITMSSFILENLHL------VVKLIENIT 215

Query: 318 EE-----LQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFIEHILWH 372
            E     ++ ++ WL+  PAGLK+N  + + + +  ++ I +W +++    P+   ++  
Sbjct: 216 FEYAIKNVRSMVIWLVDTPAGLKLNNDICKFIMKLSVWVIDVWSNFLLHCLPWTPFLVQV 275

Query: 373 VGLSACLGLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSLWRLFRGKKWNVL 432
           V +S   G ++ +++ SD ++++T HI+  Y+  +R+Y  ++  + SL +LFRGKK NVL
Sbjct: 276 VAISGFGGASLMIALISDFLSVMTIHIHLLYLASSRMYNWQLRVIYSLLQLFRGKKRNVL 335

Query: 433 RQRVDSCSYDLDQLFIGTLLFTILVFLLPTTALYCLVFTLLRLLVITVQGLIHLLVDLIN 492
           R R+DS  YDLDQL +GT+LFT+L+F LPT  ++   F L R+ V+T   +   ++  +N
Sbjct: 336 RNRIDSYEYDLDQLLLGTILFTVLIFFLPTIYVFYAAFALTRVSVMTCLAICETMLAFLN 395

Query: 493 SLPLYSLGLRLCRPYRLAAGVKFRV-----LEKEAGRPLRLLMQINPLSYNHVMHIYRLP 547
             PL+   LR+  PYR+ +G+ F +     L+++    L L     P+S   +   YR  
Sbjct: 396 HFPLFVTMLRIKDPYRIPSGLNFEIVSFEPLKQDGFATLYLNCNSKPMSLGSMFEHYRKL 455

Query: 548 RCGCHPKHSWGTLCRKLVFG 567
                  +   T    L+ G
Sbjct: 456 ARRLISHYLSKTTLISLLVG 475





>ref|NP_011732.1| (NC_001139) Participates in synthesis of
           N-acetylglucoaminylphosphatidylinositol, the first
           intermediate in synthesis of
           glycosylphosphatidylinositol (GPI) anchors; Gpi1p
           [Saccharomyces cerevisiae]
 sp|P53306|GPI1_YEAST N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN GPI1
 pir||S64539 probable membrane protein YGR216c - yeast (Saccharomyces
           cerevisiae)
 emb|CAA97243.1| (Z73001) ORF YGR216c [Saccharomyces cerevisiae]
 gb|AAB17870.1| (U66864) Gpi1p [Saccharomyces cerevisiae]
          Length = 609

 Score =  341 bits (867), Expect = 2e-92
 Identities = 103/320 (32%), Positives = 161/320 (50%), Gaps = 27/320 (8%)

Query: 272 LMRKANMLVSVLLDVALGLLLLSWLHSNNRIGQLANALVPVADRVA-----EELQHLLQW 326
            +   N +  ++ D++ GL+L + L  N       + LV  + RV      + L+ + + 
Sbjct: 275 YILLYNTIWLIINDISFGLILGAILIENR------DFLVSASHRVLKFFLYDSLKTITET 328

Query: 327 LMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFIEHILWHVGLSACL------- 379
           L   P G+K+N  L   L   FL+ I    SY      FI+ ++    LS+ L       
Sbjct: 329 LANNPLGIKLNAELANFLSELFLWVIEF--SYT----TFIKRLIDPKTLSSLLTLTIYMM 382

Query: 380 ---GLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSLWRLFRGKKWNVLRQRV 436
              G + A+S+  D  A+L+F IY FY   ++LY  ++  ++SL+ LF GKK NVLR R+
Sbjct: 383 FLVGFSFAVSLAIDFFAILSFPIYVFYRISSKLYHCQLNIMASLFNLFCGKKRNVLRNRI 442

Query: 437 DSCSYDLDQLFIGTLLFTILVFLLPTTALYCLVFTLLRLLVITVQGLIHLLVDLINSLPL 496
           D   + LDQL +GTLLF ILVFL PT   + + +T+LR+L IT++     ++ LIN  PL
Sbjct: 443 DHNYFQLDQLLLGTLLFIILVFLTPTVMAFYMSYTVLRMLTITIEIFSEAVIALINHFPL 502

Query: 497 YSLGLRLCRPYRLAAGVKFRVLEKEAGRPLRLLMQINPLSYNHVMHIYRLPRCGCHPKHS 556
           ++L LRL  P RL  G+   +    + +   L +Q NP+ +  +   Y L        + 
Sbjct: 503 FALLLRLKDPKRLPGGISIELKTTVSNKHTTLELQNNPIKFKSMFRPYNLLLSQMRTNYF 562

Query: 557 WGTLCRKLVFGELIYPWRQR 576
                RK+V GE I   R +
Sbjct: 563 SFATVRKIVRGESIMVNRNK 582





>emb|CAC10160.1| (AJ249657) N-acetylglucosamine transferase [Plasmodium falciparum]
          Length = 669

 Score =  308 bits (783), Expect = 1e-82
 Identities = 68/268 (25%), Positives = 128/268 (47%), Gaps = 5/268 (1%)

Query: 256 HLSFIFSTEKAQNPMQLMRKANMLVSVLLDVALGLLLLSWLHSNNRIGQLANALVPVADR 315
           H  F    +  QNP +  +   +L+  ++++ + + L   +        +    +    +
Sbjct: 333 HNIFRSFMKNKQNPSEYYKYREILLIRIINLIIDIFLGFLIFLLLYFNVINLHYISEKAQ 392

Query: 316 V---AEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFIEHILWH 372
           +      L  +L  L+  P G K+N      +G   +  +  W  + + +      +L  
Sbjct: 393 IFYGTSTLTSILGTLLQNPLGFKLNNNFTSFIGSILVSILDKWDLFTNTIPVNNSTVLNF 452

Query: 373 VGLSACLGLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSLWRLFRGKKWNVL 432
           VG ++ LG +  LS   D +  +T H+   Y++  ++  L    + SL+ LF GKKWN+L
Sbjct: 453 VGYTSLLGFSFFLSFVIDYLRFVTAHVTIIYLFLKKICTLFHKNMYSLYLLFNGKKWNIL 512

Query: 433 RQRVDSCSYDLDQLFIGTLLFTILVFLLPTTALYCLVFTLLRLLVITVQGLIHLLVDLIN 492
           + RVD+  Y  +++ +GT+LFTIL+FL PT  +  LVF L+ L++  +  L+ ++  +I 
Sbjct: 513 KLRVDTNYYSNEEVLLGTILFTILIFLYPTIFVLVLVFGLIYLIINRIIYLLCVMEKIIL 572

Query: 493 SLPLYSLGLRL-CRPYRLAAGVKFRVLE 519
             P Y   ++  C  Y ++ G KF   E
Sbjct: 573 YTPFYIFFIQPNCNKY-ISKGFKFTKYE 599





>emb|CAC10056.1| (AJ298995) GPI1 protein [Drosophila melanogaster]
          Length = 481

 Score =  304 bits (772), Expect = 3e-81
 Identities = 77/231 (33%), Positives = 133/231 (57%), Gaps = 2/231 (0%)

Query: 275 KANMLVSVLLDVALGLLLLSWLHSNNRIGQLANALVPVADRVAEELQHLLQWLMGAPAGL 334
           K +   +++LD  LG++L+  L S     Q  + L+ ++  V +EL  LL+ L G+P GL
Sbjct: 215 KRSRPANIVLDRILGIILMLILFS--LATQPGDFLIQISHYVIDELYGLLKVLEGSPIGL 272

Query: 335 KMNRALDQVLGRFFLYHIHLWISYIHLMSPFIEHILWHVGLSACLGLTVALSIFSDIIAL 394
           K+N  L+      F YHI LW +++  + P +  +   +G+  CLG T  +++  D+I++
Sbjct: 273 KLNIHLNNFFLDCFKYHIELWSTFLDFIEPLVRQVFLAIGMIGCLGFTFQIALLVDLISV 332

Query: 395 LTFHIYCFYVYGARLYCLKIYGLSSLWRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFT 454
           +  H +CFY+Y   LY ++  GLS LW++ RG ++N+L+ R +S +Y   QL++ T+ F+
Sbjct: 333 IGLHSHCFYIYTKVLYNVERRGLSVLWQVVRGNRYNILKGRTESHNYMNRQLYLATIFFS 392

Query: 455 ILVFLLPTTALYCLVFTLLRLLVITVQGLIHLLVDLINSLPLYSLGLRLCR 505
            ++FLLPTT +Y +VF  L+ L      + H +   +  LP+     RL R
Sbjct: 393 AILFLLPTTLVYYIVFAALKALTFATLSVFHFVRRKLMYLPIEVCIKRLLR 443





>emb|CAC10057.1| (AJ298996) GPI1 protein [Drosophila virilis]
          Length = 473

 Score =  283 bits (717), Expect = 7e-75
 Identities = 68/233 (29%), Positives = 132/233 (56%), Gaps = 2/233 (0%)

Query: 273 MRKANMLVSVLLDVALGLLLLSWLHSNNRIGQLANALVPVADRVAEELQHLLQWLMGAPA 332
             K + L ++++D ALG++L+  L +   +    + L+ ++  +  +L  LL+ L G+P 
Sbjct: 206 YTKGSRLSNIMIDRALGIILMLVLFT--LVSHPGDFLIQISHIIIHQLYSLLKVLEGSPI 263

Query: 333 GLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFIEHILWHVGLSACLGLTVALSIFSDII 392
           GLK+N  L+      F YHI LW +++ L+ P +  +   +G   CLG T  +++ +D+I
Sbjct: 264 GLKLNIHLNNFFLDCFKYHIELWSTFLDLIEPIVRQVFLAIGAFGCLGFTYQIALLADLI 323

Query: 393 ALLTFHIYCFYVYGARLYCLKIYGLSSLWRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLL 452
           +++  H +CFYVY   L  +++ GL+ LW++ RG ++N+LR R+++ +Y   QL++ T+ 
Sbjct: 324 SIVGLHAHCFYVYTKVLNNVEVKGLTVLWQVVRGNRYNILRNRIEAHNYMNRQLYLATIF 383

Query: 453 FTILVFLLPTTALYCLVFTLLRLLVITVQGLIHLLVDLINSLPLYSLGLRLCR 505
           F+ ++FL PTT +Y +VF  L+ L       +      + + P+      + +
Sbjct: 384 FSAILFLFPTTLVYYVVFATLKALTCATLATLECFRRKLLNFPIEMFLKYIKK 436





>ref|NP_502086.1| (NM_069685) F01G4.5.p [Caenorhabditis elegans]
 pir||T20486 hypothetical protein F01G4.5 - Caenorhabditis elegans
 emb|CAA92766.1| (Z68341) F01G4.5 [Caenorhabditis elegans]
          Length = 287

 Score =  262 bits (664), Expect = 1e-68
 Identities = 85/258 (32%), Positives = 118/258 (44%), Gaps = 24/258 (9%)

Query: 272 LMRKANMLVSVLLDVALGLLLLSWLHSNNRIGQLANALVPVADRVAEELQHLLQWLMGAP 331
           L+R  +    +LLDVA  + L +    N       N        VA+ L   + WL   P
Sbjct: 20  LVRTFSFTRLLLLDVAFSIYLWNIWTPNWE--WTVNEFWDQTGNVADNLNGTITWLRSNP 77

Query: 332 AGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFIEHILWHVGLSACLGL---------- 381
           AGLK+N  +++ L  FF YHI+LW S +     F  +  +H   SA +G           
Sbjct: 78  AGLKLNTPVNETLAWFFTYHIYLWTSMLFCFQKFAFN--FHNDFSAFIGFLRSDAFFRFI 135

Query: 382 ----TVALSIFS----DIIALLTFHIYCFYVYGARLYCLKIYGLSSLWRLFRGKKWNVLR 433
                  +S FS    D   +   H  CF  Y  +L  L  Y L+ LW L RGKKWN LR
Sbjct: 136 AYSLIGGISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLTVLWSLVRGKKWNPLR 195

Query: 434 QRVDSCSYDLDQLFIGTLLFTILVFLLPTTALYCLVFTLLRLLVITVQGLIHLLVDLINS 493
           +R D+   D  Q F+ T LF IL+F+LPT  +Y +VF  LRL V  +Q +++        
Sbjct: 196 ERKDTVILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLRLAVSALQTVLYFFATW--P 253

Query: 494 LPLYSLGLRLCRPYRLAA 511
             L++L   L   Y   A
Sbjct: 254 FQLFALEKHLAEKYGKPA 271





>ref|NP_191276.1| (NM_115577) putative protein [Arabidopsis thaliana]
 pir||T47777 hypothetical protein F24I3.250 - Arabidopsis thaliana
 emb|CAB72187.1| (AL138655) putative protein [Arabidopsis thaliana]
          Length = 680

 Score =  238 bits (603), Expect = 2e-61
 Identities = 76/236 (32%), Positives = 121/236 (51%), Gaps = 4/236 (1%)

Query: 286 VALGLLLLSWLHSNNRIG--QLANALVPVADRVAEE-LQHLLQWLMGAPAGLKMNRALDQ 342
           +A+ L+L + +          + + +   A       L+    WLMG PAG K+N  L  
Sbjct: 333 MAVDLVLGNLIGLGLLFNTESVCSFVFDFAKEFTNGILRSGSVWLMGVPAGFKLNTELAG 392

Query: 343 VLGRFFLYHIHLWISYIHLMSPFIEHILWHVG-LSACLGLTVALSIFSDIIALLTFHIYC 401
           VLG   L  I +W +    M+ FI  ++  +  L    G TV+ +   D+I   T HI  
Sbjct: 393 VLGMVSLNVIQIWSTLWVFMASFIFCLIRVIAILGITFGATVSAAFVIDVITFATLHIMA 452

Query: 402 FYVYGARLYCLKIYGLSSLWRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILVFLLP 461
            +     +Y  +I  L++LWRLFRG+K N LRQR+DS  Y + Q  +G+LLFT L+ LLP
Sbjct: 453 LHWAITLVYSHQIQALAALWRLFRGRKLNPLRQRMDSYGYTVKQHVVGSLLFTPLLLLLP 512

Query: 462 TTALYCLVFTLLRLLVITVQGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRV 517
           TT+++ + FT+    + ++  LI   + +I++ P   + + L R  R   GV F +
Sbjct: 513 TTSVFYIFFTITSTTINSICMLIEFAISVIHATPYAEVMIWLVRRKRFPCGVWFEM 568





>pir||T45794 hypothetical protein F28O9.10 - Arabidopsis thaliana  (fragment)
 emb|CAB68122.1| (AL137080) hypothetical protein fragment [Arabidopsis thaliana]
          Length = 143

 Score =  124 bits (310), Expect = 4e-27
 Identities = 41/143 (28%), Positives = 65/143 (44%), Gaps = 4/143 (2%)

Query: 286 VALGLLLLSWLHSNNRIG--QLANALVPVADRVAEE-LQHLLQWLMGAPAGLKMNRALDQ 342
           +A+ L+L + +          + + +   A       L+    WLMG PAG K+N  L  
Sbjct: 1   MAVDLVLGNLIGLGLLFNTESVCSFVFDFAKEFTNGILRSGSVWLMGVPAGFKLNTELAG 60

Query: 343 VLGRFFLYHIHLWISYIHLMSPFIEHILWHVG-LSACLGLTVALSIFSDIIALLTFHIYC 401
           VLG   L  I +W +    M+ FI  ++  +  L    G TV+ +   D+I   T HI  
Sbjct: 61  VLGMVSLNVIQIWSTLWVFMASFIFCLIRVIAILGITFGATVSAAFVIDVITFATLHIMA 120

Query: 402 FYVYGARLYCLKIYGLSSLWRLF 424
            +     +Y  +I  L++LWRLF
Sbjct: 121 LHWAITLVYSHQIQALAALWRLF 143





CPU time:   156.05 user secs.	    2.49 sys. secs	  158.54 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.312    0.164    0.514 

Gapped
Lambda     K      H
   0.270   0.0574    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 391030670
Number of Sequences: 887402
Number of extensions: 19300470
Number of successful extensions: 73262
Number of sequences better than 10.0: 112
Number of HSP's better than 10.0 without gapping: 22
Number of HSP's successfully gapped in prelim test: 92
Number of HSP's that attempted gapping in prelim test: 73120
Number of HSP's gapped (non-prelim): 189
length of query: 581
length of database: 277,845,442
effective HSP length: 51
effective length of query: 530
effective length of database: 232,587,940
effective search space: 123271608200
effective search space used: 123271608200
T: 11
A: 40
X1: 15 ( 6.7 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (20.6 bits)
S2: 76 (33.7 bits)