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GPI Anchor Biosynthesis Report: CAC10160.1 (PIG-Q family, Plasmodium falciparum)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query=
         (669 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!


Results of PSI-Blast iteration 3

Distribution of 21 Blast Hits on the Query Sequence


Sequences with E-value BETTER than threshold (0.002)

emb|CAC10160.1| (AJ249657) N-acetylglucosamine transferase [Plas... 798 0.0
sp|O14357|GPI1_SCHPO N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL B... 461 e-128
ref|XP_027458.2| (XM_027458) similar to phosphatidylinositol gly... 454 e-126
gb|AAC49650.1| (U77355) Gpi1+ [Schizosaccharomyces pombe] 452 e-126
emb|CAB56148.1| (Z98883) c407A10.1 (GPI1 (N-acetylglucosaminyl t... 451 e-125
gb|AAK61235.1|AE006464_3 (AE006464) N-acetylglucosamyl transfera... 445 e-124
ref|NP_004195.1| (NM_004204) phosphatidylinositol glycan, class ... 443 e-123
dbj|BAA84658.1| (AB008921) mGpi1 [Mus musculus] >gi|15679960|gb|... 431 e-119
ref|NP_035952.1| (NM_011822) glycosylphosphatidylinositol 1 homo... 428 e-118
ref|NP_191276.1| (NM_115577) putative protein [Arabidopsis thali... 423 e-117
ref|NP_011732.1| (NC_001139) Participates in synthesis of N-acet... 421 e-116
gb|AAC79574.1| (AF030178) N-acetylglucosaminyl transferase compo... 420 e-116
gb|AAH03917.1|AAH03917 (BC003917) Unknown (protein for IMAGE:348... 396 e-109
emb|CAC10057.1| (AJ298996) GPI1 protein [Drosophila virilis] 345 2e-93
emb|CAC10056.1| (AJ298995) GPI1 protein [Drosophila melanogaster] 327 3e-88
ref|NP_502086.1| (NM_069685) F01G4.5.p [Caenorhabditis elegans] ... 230 6e-59
pir||T45794 hypothetical protein F28O9.10 - Arabidopsis thaliana... 156 1e-36
dbj|BAB30464.1| (AK016852) data source:MGD, source key:MGI:13331... 81 5e-14
emb|CAB68123.2| (AL137080) hypothetical protein [Arabidopsis tha... 80 1e-13
pir||T45795 hypothetical protein F28O9.20 - Arabidopsis thaliana 78 7e-13
gb|AAH10094.1|AAH10094 (BC010094) Similar to phosphatidylinosito... 64 6e-09
Alignments
>emb|CAC10160.1| (AJ249657) N-acetylglucosamine transferase [Plasmodium falciparum]
          Length = 669

 Score =  798 bits (2039), Expect = 0.0
 Identities = 659/659 (100%), Positives = 659/659 (100%)

Query: 1   MCTTKNEDNNKVNYLYLIYYNNIPIFKKKSGNEQNISALFILYDVKKYVYNMVHDHVNTL 60
           MCTTKNEDNNKVNYLYLIYYNNIPIFKKKSGNEQNISALFILYDVKKYVYNMVHDHVNTL
Sbjct: 1   MCTTKNEDNNKVNYLYLIYYNNIPIFKKKSGNEQNISALFILYDVKKYVYNMVHDHVNTL 60

Query: 61  VLEAFRREDIIKKIKVKEKQNNNDKNKESNIEKDKNEQTKFTDIYDTNSKSDKDIQKNNM 120
           VLEAFRREDIIKKIKVKEKQNNNDKNKESNIEKDKNEQTKFTDIYDTNSKSDKDIQKNNM
Sbjct: 61  VLEAFRREDIIKKIKVKEKQNNNDKNKESNIEKDKNEQTKFTDIYDTNSKSDKDIQKNNM 120

Query: 121 NDGDSNNKNSSLFIDPFESDSYEKNNFSNEKCAFQNVDKSKKDKEHIYSENITPSSSNNN 180
           NDGDSNNKNSSLFIDPFESDSYEKNNFSNEKCAFQNVDKSKKDKEHIYSENITPSSSNNN
Sbjct: 121 NDGDSNNKNSSLFIDPFESDSYEKNNFSNEKCAFQNVDKSKKDKEHIYSENITPSSSNNN 180

Query: 181 NDNNKENDCDKEQLDKYNKDKENKLKLNDKDEYISFNFIEDKLTESFHMNQIIHLINKKC 240
           NDNNKENDCDKEQLDKYNKDKENKLKLNDKDEYISFNFIEDKLTESFHMNQIIHLINKKC
Sbjct: 181 NDNNKENDCDKEQLDKYNKDKENKLKLNDKDEYISFNFIEDKLTESFHMNQIIHLINKKC 240

Query: 241 VFTKCLENYKNRYFVLKKEEILKKKKKQKKMSIFSYIVSIILFFTYIISLINSCLYYIIC 300
           VFTKCLENYKNRYFVLKKEEILKKKKKQKKMSIFSYIVSIILFFTYIISLINSCLYYIIC
Sbjct: 241 VFTKCLENYKNRYFVLKKEEILKKKKKQKKMSIFSYIVSIILFFTYIISLINSCLYYIIC 300

Query: 301 TPKLFSEYIFSKKCDGYLQNSAYPKFIFPSEWHNIFRSFMKNKQNPSEYYKYREILLIRI 360
           TPKLFSEYIFSKKCDGYLQNSAYPKFIFPSEWHNIFRSFMKNKQNPSEYYKYREILLIRI
Sbjct: 301 TPKLFSEYIFSKKCDGYLQNSAYPKFIFPSEWHNIFRSFMKNKQNPSEYYKYREILLIRI 360

Query: 361 INLIIDIFLGFLIFLLLYFNVINLHYISEKAQIFYGTSTLTSILGTLLQNPLGFKLNNNF 420
           INLIIDIFLGFLIFLLLYFNVINLHYISEKAQIFYGTSTLTSILGTLLQNPLGFKLNNNF
Sbjct: 361 INLIIDIFLGFLIFLLLYFNVINLHYISEKAQIFYGTSTLTSILGTLLQNPLGFKLNNNF 420

Query: 421 TSFIGSILVSILDKWDLFTNTIPVNNSTVLNFVGYTSLLGFSFFLSFVIDYLRFVTAHVT 480
           TSFIGSILVSILDKWDLFTNTIPVNNSTVLNFVGYTSLLGFSFFLSFVIDYLRFVTAHVT
Sbjct: 421 TSFIGSILVSILDKWDLFTNTIPVNNSTVLNFVGYTSLLGFSFFLSFVIDYLRFVTAHVT 480

Query: 481 IIYLFLKKICTLFHKNMYSLYLLFNGKKWNILKLRVDTNYYSNEEVLLGTILFTILIFLY 540
           IIYLFLKKICTLFHKNMYSLYLLFNGKKWNILKLRVDTNYYSNEEVLLGTILFTILIFLY
Sbjct: 481 IIYLFLKKICTLFHKNMYSLYLLFNGKKWNILKLRVDTNYYSNEEVLLGTILFTILIFLY 540

Query: 541 PTIFVLVLVFGLIYLIINRIIYLLCVMEKIILYTPFYIFFIQPNCNKYISKGFKFTKYEV 600
           PTIFVLVLVFGLIYLIINRIIYLLCVMEKIILYTPFYIFFIQPNCNKYISKGFKFTKYEV
Sbjct: 541 PTIFVLVLVFGLIYLIINRIIYLLCVMEKIILYTPFYIFFIQPNCNKYISKGFKFTKYEV 600

Query: 601 GEHELLKRYPTNSYLLLEKIHFLFFDKIKLFINIFLYFKNLESMSSYSYIFISFCFCLL 659
           GEHELLKRYPTNSYLLLEKIHFLFFDKIKLFINIFLYFKNLESMSSYSYIFISFCFCLL
Sbjct: 601 GEHELLKRYPTNSYLLLEKIHFLFFDKIKLFINIFLYFKNLESMSSYSYIFISFCFCLL 659
>sp|O14357|GPI1_SCHPO N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN GPI1
 pir||T40185 n-acetylglucosaminyl-phosphatidylinositol biosynthetic protein gpi1
           - fission yeast (Schizosaccharomyces pombe)
 emb|CAB10806.1| (Z97992) n-acetylglucosaminyl-phosphatidylinositol biosynthetic
           protein gpi1. [Schizosaccharomyces pombe]
          Length = 653

 Score =  461 bits (1176), Expect = e-128
 Identities = 95/407 (23%), Positives = 175/407 (42%), Gaps = 37/407 (9%)

Query: 197 YNKDKENKLKLNDKDEYISFNFIEDKLTESFHMNQIIHLINKKCVFTKCLENYKNRYFVL 256
           + + KEN  K + +   I        L +SF +  + H  N K          KN Y   
Sbjct: 179 FPRRKENSWKRSLRSGLIEL------LNQSFEVRMLTHENNNK----------KNSYVFR 222

Query: 257 KKEEILKKKKKQKKMSIFSYIVSIILFFTYIISLINSCLYYII------CTPKLFSEYIF 310
             + +          S F +  S+  +F  ++ +IN  +   I       +  +   ++ 
Sbjct: 223 LFDRV--------SSSTFYFFNSLFAYFIILLRIINEVILLAINYRPIPLSYNMMDIFVS 274

Query: 311 SKKCDGYLQNSAYPKFIFPSEWHNIFRSFMKNK-QNPSEYYKYREILLIRIINLIIDIFL 369
           +++ D  LQ + +    +   W  +FR   +   ++  EY ++   L +   ++I  I +
Sbjct: 275 ARQVDLRLQQACFWPVQYMKLW--VFRKSKRVAIEDYKEYIRFYNNLWLVANDMIFGITM 332

Query: 370 GFLIFLLLYFNVINLHYISEKAQIFYGTSTLTSILGTLLQNPLGFKLNNNFTSFIGSILV 429
              I   L+  V     + E     Y    + S++  L+  P G KLNN+   FI  + V
Sbjct: 333 SSFILENLHLVV----KLIENITFEYAIKNVRSMVIWLVDTPAGLKLNNDICKFIMKLSV 388

Query: 430 SILDKWDLFTNTIPVNNSTVLNFVGYTSLLGFSFFLSFVIDYLRFVTAHVTIIYLFLKKI 489
            ++D W  F          ++  V  +   G S  ++ + D+L  +T H+ ++YL   ++
Sbjct: 389 WVIDVWSNFLLHCLPWTPFLVQVVAISGFGGASLMIALISDFLSVMTIHIHLLYLASSRL 448

Query: 490 CTLFHKNMYSLYLLFNGKKWNILKLRVDTNYYSNEEVLLGTILFTILIFLYPTIFVLVLV 549
                + +YSL  LF GKK N+L+ R+D+  Y  +++LLGTILFT+LIF  PTI+V    
Sbjct: 449 YNWQLRVIYSLLQLFRGKKRNVLRNRIDSYEYDLDQLLLGTILFTVLIFFLPTIYVFYAA 508

Query: 550 FGLIYLIINRIIYLLCVMEKIILYTPFYIFFIQPNCNKYISKGFKFT 596
           F L  + +   + +   M   + + P ++  ++      I  G  F 
Sbjct: 509 FALTRVSVMTCLAICETMLAFLNHFPLFVTMLRIKDPYRIPSGLNFE 555
>ref|XP_027458.2| (XM_027458) similar to phosphatidylinositol glycan, class Q [Homo
           sapiens]
 gb|AAH06377.1|AAH06377 (BC006377) Similar to phosphatidylinositol glycan, class Q [Homo
           sapiens]
          Length = 760

 Score =  454 bits (1157), Expect = e-126
 Identities = 70/312 (22%), Positives = 139/312 (44%), Gaps = 14/312 (4%)

Query: 280 IILFFTYIISLINSCLYYIICTPKLFSEYIFSKKCDGYLQNSAYPKFIFPSEWHNIFRSF 339
           I L    ++SL+++         KL+       K     +   +         H      
Sbjct: 212 ICLLLASLLSLVSAVSACR--VFKLWPLSFLGSKLS-TCEQLRH------RLEHLTLIFS 262

Query: 340 MKNKQNPSEYYKYREILLIRIINLIIDIFLGFLIFLLLYFNVINLHYISEKAQIF-YGTS 398
            +  +NP++  +        + ++++D+ LG ++   L+      H       +  +   
Sbjct: 263 TRKAENPAQLMRKAN----TVASVLLDVALGLMLLSWLHGRSRIGHLADALVPVADHVAE 318

Query: 399 TLTSILGTLLQNPLGFKLNNNFTSFIGSILVSILDKWDLFTNTIPVNNSTVLNFVGYTSL 458
            L  +L  L+  P G K+N      +G   +  +  W  + + +      +L  VG ++ 
Sbjct: 319 ELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWHVGLSAC 378

Query: 459 LGFSFFLSFVIDYLRFVTAHVTIIYLFLKKICTLFHKNMYSLYLLFNGKKWNILKLRVDT 518
           LG +  LS + D +  +T H+   Y++  ++  L    + SL+ LF GKKWN+L+ RVD+
Sbjct: 379 LGLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSLWRLFRGKKWNVLRQRVDS 438

Query: 519 NYYSNEEVLLGTILFTILIFLYPTIFVLVLVFGLIYLIINRIIYLLCVMEKIILYTPFYI 578
             Y  +++ +GT+LFTIL+FL PT  +  LVF L+ L++  +  L+ ++  +I   P Y 
Sbjct: 439 CSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDLINSLPLYS 498

Query: 579 FFIQPNCNKYIS 590
             ++      ++
Sbjct: 499 LGLRLCRPYRLA 510
>gb|AAC49650.1| (U77355) Gpi1+ [Schizosaccharomyces pombe]
          Length = 517

 Score =  452 bits (1152), Expect = e-126
 Identities = 95/407 (23%), Positives = 175/407 (42%), Gaps = 37/407 (9%)

Query: 197 YNKDKENKLKLNDKDEYISFNFIEDKLTESFHMNQIIHLINKKCVFTKCLENYKNRYFVL 256
           + + KEN  K + +   I        L +SF +  + H  N K          KN Y   
Sbjct: 43  FPRRKENSWKRSLRSGLIEL------LNQSFEVRMLTHENNNK----------KNSYVFR 86

Query: 257 KKEEILKKKKKQKKMSIFSYIVSIILFFTYIISLINSCLYYII------CTPKLFSEYIF 310
             + +          S F +  S+  +F  ++ +IN  +   I       +  +   ++ 
Sbjct: 87  LFDRV--------SSSTFYFFNSLFAYFIILLRIINEVILLAINYRPIPLSYNMMDIFVS 138

Query: 311 SKKCDGYLQNSAYPKFIFPSEWHNIFRSFMKNK-QNPSEYYKYREILLIRIINLIIDIFL 369
           +++ D  LQ + +    +   W  +FR   +   ++  EY ++   L +   ++I  I +
Sbjct: 139 ARQVDLRLQQACFWPVQYMKLW--VFRKSKRVAIEDYKEYIRFYNNLWLVANDMIFGITM 196

Query: 370 GFLIFLLLYFNVINLHYISEKAQIFYGTSTLTSILGTLLQNPLGFKLNNNFTSFIGSILV 429
              I   L+  V     + E     Y    + S++  L+  P G KLNN+   FI  + V
Sbjct: 197 SSFILENLHLVV----KLIENITFEYAIKNVRSMVIWLVDTPAGLKLNNDICKFIMKLSV 252

Query: 430 SILDKWDLFTNTIPVNNSTVLNFVGYTSLLGFSFFLSFVIDYLRFVTAHVTIIYLFLKKI 489
            ++D W  F          ++  V  +   G S  ++ + D+L  +T H+ ++YL   ++
Sbjct: 253 WVIDVWSNFLLHCLPWTPFLVQVVAISGFGGASLMIALISDFLSVMTIHIHLLYLASSRM 312

Query: 490 CTLFHKNMYSLYLLFNGKKWNILKLRVDTNYYSNEEVLLGTILFTILIFLYPTIFVLVLV 549
                + +YSL  LF GKK N+L+ R+D+  Y  +++LLGTILFT+LIF  PTI+V    
Sbjct: 313 YNWQLRVIYSLLQLFRGKKRNVLRNRIDSYEYDLDQLLLGTILFTVLIFFLPTIYVFYAA 372

Query: 550 FGLIYLIINRIIYLLCVMEKIILYTPFYIFFIQPNCNKYISKGFKFT 596
           F L  + +   + +   M   + + P ++  ++      I  G  F 
Sbjct: 373 FALTRVSVMTCLAICETMLAFLNHFPLFVTMLRIKDPYRIPSGLNFE 419
>emb|CAB56148.1| (Z98883) c407A10.1 (GPI1 (N-acetylglucosaminyl transferase
           component)) [Homo sapiens]
          Length = 583

 Score =  451 bits (1149), Expect = e-125
 Identities = 75/325 (23%), Positives = 146/325 (44%), Gaps = 14/325 (4%)

Query: 280 IILFFTYIISLINSCLYYIICTPKLFSEYIFSKKCDGYLQNSAYPKFIFPSEWHNIFRSF 339
           I L    ++SL+++         KL+       K     +   +         H      
Sbjct: 212 ICLLLASLLSLVSAVSACRRRVFKLWPLSFLGSKLS-TCEQLRH------RLEHLTLIFS 264

Query: 340 MKNKQNPSEYYKYREILLIRIINLIIDIFLGFLIFLLLYFNVINLHYISEKAQIF-YGTS 398
            +  +NP++  +        + ++++D+ LG ++   L+      H       +  +   
Sbjct: 265 TRKAENPAQLMRKAN----TVASVLLDVALGLMLLSWLHGRSRIGHLADALVPVADHVAE 320

Query: 399 TLTSILGTLLQNPLGFKLNNNFTSFIGSILVSILDKWDLFTNTIPVNNSTVLNFVGYTSL 458
            L  +L  L+  P G K+N      +G   +  +  W  + + +      +L  VG ++ 
Sbjct: 321 ELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWHVGLSAC 380

Query: 459 LGFSFFLSFVIDYLRFVTAHVTIIYLFLKKICTLFHKNMYSLYLLFNGKKWNILKLRVDT 518
           LG +  LS + D +  +T H+   Y++  ++  L    + SL+ LF GKKWN+L+ RVD+
Sbjct: 381 LGLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSLWRLFRGKKWNVLRQRVDS 440

Query: 519 NYYSNEEVLLGTILFTILIFLYPTIFVLVLVFGLIYLIINRIIYLLCVMEKIILYTPFYI 578
             Y  +++ +GT+LFTIL+FL PT  +  LVF L+ L++  +  L+ ++  +I   P Y 
Sbjct: 441 CSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDLINSLPLYS 500

Query: 579 FFIQPNCNKYISKGFKFT--KYEVG 601
             ++      ++ G KF   ++E G
Sbjct: 501 LGLRLCRPYRLAAGVKFRVLRHEAG 525
>gb|AAK61235.1|AE006464_3 (AE006464) N-acetylglucosamyl transferase component GPI1 [Homo
           sapiens]
          Length = 581

 Score =  445 bits (1134), Expect = e-124
 Identities = 75/325 (23%), Positives = 146/325 (44%), Gaps = 16/325 (4%)

Query: 280 IILFFTYIISLINSCLYYIICTPKLFSEYIFSKKCDGYLQNSAYPKFIFPSEWHNIFRSF 339
           I L    ++SL+++         KL+       K     +   +         H      
Sbjct: 212 ICLLLASLLSLVSAVSACR--VFKLWPLSFLGSKLS-TCEQLRH------RLEHLTLIFS 262

Query: 340 MKNKQNPSEYYKYREILLIRIINLIIDIFLGFLIFLLLYFNVINLHYISEKAQIF-YGTS 398
            +  +NP++  +        + ++++D+ LG ++   L+      H       +  +   
Sbjct: 263 TRKAENPAQLMRKAN----TVASVLLDVALGLMLLSWLHGRSRIGHLADALVPVADHVAE 318

Query: 399 TLTSILGTLLQNPLGFKLNNNFTSFIGSILVSILDKWDLFTNTIPVNNSTVLNFVGYTSL 458
            L  +L  L+  P G K+N      +G   +  +  W  + + +      +L  VG ++ 
Sbjct: 319 ELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWHVGLSAC 378

Query: 459 LGFSFFLSFVIDYLRFVTAHVTIIYLFLKKICTLFHKNMYSLYLLFNGKKWNILKLRVDT 518
           LG +  LS + D +  +T H+   Y++  ++  L    + SL+ LF GKKWN+L+ RVD+
Sbjct: 379 LGLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSLWRLFRGKKWNVLRQRVDS 438

Query: 519 NYYSNEEVLLGTILFTILIFLYPTIFVLVLVFGLIYLIINRIIYLLCVMEKIILYTPFYI 578
             Y  +++ +GT+LFTIL+FL PT  +  LVF L+ L++  +  L+ ++  +I   P Y 
Sbjct: 439 CSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDLINSLPLYS 498

Query: 579 FFIQPNCNKYISKGFKFT--KYEVG 601
             ++      ++ G KF   ++E G
Sbjct: 499 LGLRLCRPYRLAAGVKFRVLRHEAG 523
>ref|NP_004195.1| (NM_004204) phosphatidylinositol glycan, class Q;
           N-acetylglucosaminyl transferase component Gpi1 [Homo
           sapiens]
 gb|AAC32661.1| (AF030177) N-acetylglucosaminyl transferase component Gpi1 [Homo
           sapiens]
 dbj|BAA24948.1| (AB003723) GPI1 [Homo sapiens]
          Length = 581

 Score =  443 bits (1128), Expect = e-123
 Identities = 73/320 (22%), Positives = 142/320 (43%), Gaps = 14/320 (4%)

Query: 280 IILFFTYIISLINSCLYYIICTPKLFSEYIFSKKCDGYLQNSAYPKFIFPSEWHNIFRSF 339
           I L    ++SL+++         KL+       K     +   +         H      
Sbjct: 212 ICLLLASLLSLVSAVSACR--VFKLWPLSFLGSKLS-TCEQLRH------RLEHLTLIFS 262

Query: 340 MKNKQNPSEYYKYREILLIRIINLIIDIFLGFLIFLLLYFNVINLHYISEKAQIF-YGTS 398
            +  +NP++  +        + ++++D+ LG ++   L+      H       +  +   
Sbjct: 263 TRKAENPAQLMRKAN----TVASVLLDVALGLMLLSWLHGRSRIGHLADALVPVADHVAE 318

Query: 399 TLTSILGTLLQNPLGFKLNNNFTSFIGSILVSILDKWDLFTNTIPVNNSTVLNFVGYTSL 458
            L  +L  L+  P G K+N      +G   +  +  W  + + +      +L  VG ++ 
Sbjct: 319 ELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFVEHILWHVGLSAC 378

Query: 459 LGFSFFLSFVIDYLRFVTAHVTIIYLFLKKICTLFHKNMYSLYLLFNGKKWNILKLRVDT 518
           LG +  LS + D +  +T H+   Y++  ++  L    + SL+ LF GKKWN+L+ RVD+
Sbjct: 379 LGLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSLWRLFRGKKWNVLRQRVDS 438

Query: 519 NYYSNEEVLLGTILFTILIFLYPTIFVLVLVFGLIYLIINRIIYLLCVMEKIILYTPFYI 578
             Y  +++ +GT+LFTIL+FL PT  +  LVF L+ L++  +  L+ ++  +I   P Y 
Sbjct: 439 CSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDLINSLPLYS 498

Query: 579 FFIQPNCNKYISKGFKFTKY 598
             ++      ++ G KF   
Sbjct: 499 LGLRLCRPYRLAAGVKFRVL 518
>dbj|BAA84658.1| (AB008921) mGpi1 [Mus musculus]
 gb|AAH14287.1|AAH14287 (BC014287) Similar to glycosylphosphatidylinositol 1 homolog
           (human) [Mus musculus]
          Length = 581

 Score =  431 bits (1099), Expect = e-119
 Identities = 78/321 (24%), Positives = 144/321 (44%), Gaps = 14/321 (4%)

Query: 280 IILFFTYIISLINSCLYYIICTPKLFSEYIFSKKCDGYLQNSAYPKFIFPSEWHNIFRSF 339
           + L    ++SLI++     +   KL+       K     +   +         H  F   
Sbjct: 212 VCLLLASLLSLISAASACRL--WKLWPLSFIRSKLS-TCEQLHH------RLKHLSFIFS 262

Query: 340 MKNKQNPSEYYKYREILLIRIINLIIDIFLGFLIFLLLYFNVINLHYISEKAQIF-YGTS 398
            +  QNP +  +   +L+     +++D+ LG L+   L+ N       +    +      
Sbjct: 263 TEKAQNPMQLMRKANMLVS----VLLDVALGLLLLSWLHSNNRIGQLANALVPVADRVAE 318

Query: 399 TLTSILGTLLQNPLGFKLNNNFTSFIGSILVSILDKWDLFTNTIPVNNSTVLNFVGYTSL 458
            L  +L  L+  P G K+N      +G   +  +  W  + + +      +L  VG ++ 
Sbjct: 319 ELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFIEHILWHVGLSAC 378

Query: 459 LGFSFFLSFVIDYLRFVTAHVTIIYLFLKKICTLFHKNMYSLYLLFNGKKWNILKLRVDT 518
           LG +  LS   D +  +T H+   Y++  ++  L    + SL+ LF GKKWN+L+ RVD+
Sbjct: 379 LGLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSLWRLFRGKKWNVLRQRVDS 438

Query: 519 NYYSNEEVLLGTILFTILIFLYPTIFVLVLVFGLIYLIINRIIYLLCVMEKIILYTPFYI 578
             Y  +++ +GT+LFTIL+FL PT  +  LVF L+ L++  +  L+ ++  +I   P Y 
Sbjct: 439 CSYDLDQLFIGTLLFTILVFLLPTTALYYLVFTLLRLLVITVQGLIHLLVDLINSLPLYS 498

Query: 579 FFIQPNCNKYISKGFKFTKYE 599
             ++      ++ G KF   E
Sbjct: 499 LGLRLCRPYRLAAGVKFRVLE 519
>ref|NP_035952.1| (NM_011822) glycosylphosphatidylinositol 1 homolog (human);
           glycosylphosphatidylinositol homolog (S. cerevisiae)
           [Mus musculus]
 dbj|BAA23615.1| (AB008895) mGpi1p [Mus musculus]
          Length = 581

 Score =  428 bits (1090), Expect = e-118
 Identities = 78/321 (24%), Positives = 144/321 (44%), Gaps = 14/321 (4%)

Query: 280 IILFFTYIISLINSCLYYIICTPKLFSEYIFSKKCDGYLQNSAYPKFIFPSEWHNIFRSF 339
           + L    ++SLI++     +   KL+       K     +   +         H  F   
Sbjct: 212 VCLLLASLLSLISAASACRL--WKLWPLSFIRSKLS-TCEQLHH------RLKHLSFIFS 262

Query: 340 MKNKQNPSEYYKYREILLIRIINLIIDIFLGFLIFLLLYFNVINLHYISEKAQIF-YGTS 398
            +  QNP +  +   +L+     +++D+ LG L+   L+ N       +    +      
Sbjct: 263 TEKAQNPMQLMRKANMLVS----VLLDVALGLLLLSWLHSNNRIGQLANALVPVADRVAE 318

Query: 399 TLTSILGTLLQNPLGFKLNNNFTSFIGSILVSILDKWDLFTNTIPVNNSTVLNFVGYTSL 458
            L  +L  L+  P G K+N      +G   +  +  W  + + +      +L  VG ++ 
Sbjct: 319 ELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFIEHILWHVGLSAC 378

Query: 459 LGFSFFLSFVIDYLRFVTAHVTIIYLFLKKICTLFHKNMYSLYLLFNGKKWNILKLRVDT 518
           LG +  LS   D +  +T H+   Y++  ++  L    + SL+ LF GKKWN+L+ RVD+
Sbjct: 379 LGLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSLWRLFRGKKWNVLRQRVDS 438

Query: 519 NYYSNEEVLLGTILFTILIFLYPTIFVLVLVFGLIYLIINRIIYLLCVMEKIILYTPFYI 578
             Y  +++ +GT+LFTIL+FL PT  +  LVF L+ L++  +  L+ ++  +I   P Y 
Sbjct: 439 CSYDLDQLFIGTLLFTILVFLLPTTALYCLVFTLLRLLVITVQGLIHLLVDLINSLPLYS 498

Query: 579 FFIQPNCNKYISKGFKFTKYE 599
             ++      ++ G KF   E
Sbjct: 499 LGLRLCRPYRLAAGVKFRVLE 519
>ref|NP_191276.1| (NM_115577) putative protein [Arabidopsis thaliana]
 pir||T47777 hypothetical protein F24I3.250 - Arabidopsis thaliana
 emb|CAB72187.1| (AL138655) putative protein [Arabidopsis thaliana]
          Length = 680

 Score =  423 bits (1078), Expect = e-117
 Identities = 95/422 (22%), Positives = 172/422 (40%), Gaps = 34/422 (8%)

Query: 204 KLKLNDKDEYISFNFIEDKLTESFHMNQIIHLINKKCVFTKCLENYKNRYFVLKKEEILK 263
            L  ++          + K  +  H  + ++ +    +   C    K  Y  +      +
Sbjct: 169 SLSFSNSSPQTKAPLKKPKWVDDLHNRKPLNEMETVILSLNCAAAAKIAYKKIS----TQ 224

Query: 264 KKKKQKKMSIFSYIVSIIL-FFTYIISLINSCLY------YIICTPKLFS-EYIFSKKC- 314
            +   +  SI   I S+       I+  ++S  Y      Y++ +  +FS  +I S++  
Sbjct: 225 LETSSQNFSISYLISSLTWRLLATILGSLSSLYYSLAQFFYLLSSFLIFSWVHIASRRVL 284

Query: 315 -------DGYLQNSAYPKFIFPSEWHNIFRSFMKNKQNPSEYYKYREILLIRIINLIIDI 367
                          Y   IF  E   +  S +K+ +   E    R         + +D+
Sbjct: 285 KNTWINFRIRSCQILYWP-IFLEEIDMMSISCVKHAE---EAALQRHSTWS---AMAVDL 337

Query: 368 FLGFLIFLLLYFNVINL-HYISEKAQIFYGTSTLTSILGTLLQNPLGFKLNNNFTSFIGS 426
            LG LI L L FN  ++  ++ + A+ F     L S    L+  P GFKLN      +G 
Sbjct: 338 VLGNLIGLGLLFNTESVCSFVFDFAKEF-TNGILRSGSVWLMGVPAGFKLNTELAGVLGM 396

Query: 427 ILVSILDKWDL---FTNTIPVNNSTVLNFVGYTSLLGFSFFLSFVIDYLRFVTAHVTIIY 483
           + ++++  W     F  +       V+  +G T   G +   +FVID + F T H+  ++
Sbjct: 397 VSLNVIQIWSTLWVFMASFIFCLIRVIAILGIT--FGATVSAAFVIDVITFATLHIMALH 454

Query: 484 LFLKKICTLFHKNMYSLYLLFNGKKWNILKLRVDTNYYSNEEVLLGTILFTILIFLYPTI 543
             +  + +   + + +L+ LF G+K N L+ R+D+  Y+ ++ ++G++LFT L+ L PT 
Sbjct: 455 WAITLVYSHQIQALAALWRLFRGRKLNPLRQRMDSYGYTVKQHVVGSLLFTPLLLLLPTT 514

Query: 544 FVLVLVFGLIYLIINRIIYLLCVMEKIILYTPFYIFFIQPNCNKYISKGFKFTKYEVGEH 603
            V  + F +    IN I  L+     +I  TP+    I     K    G  F     GEH
Sbjct: 515 SVFYIFFTITSTTINSICMLIEFAISVIHATPYAEVMIWLVRRKRFPCGVWFEMEHCGEH 574

Query: 604 EL 605
            L
Sbjct: 575 IL 576
>ref|NP_011732.1| (NC_001139) Participates in synthesis of
           N-acetylglucoaminylphosphatidylinositol, the first
           intermediate in synthesis of
           glycosylphosphatidylinositol (GPI) anchors; Gpi1p
           [Saccharomyces cerevisiae]
 sp|P53306|GPI1_YEAST N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN GPI1
 pir||S64539 probable membrane protein YGR216c - yeast (Saccharomyces
           cerevisiae)
 emb|CAA97243.1| (Z73001) ORF YGR216c [Saccharomyces cerevisiae]
 gb|AAB17870.1| (U66864) Gpi1p [Saccharomyces cerevisiae]
          Length = 609

 Score =  421 bits (1073), Expect = e-116
 Identities = 101/426 (23%), Positives = 179/426 (41%), Gaps = 43/426 (10%)

Query: 193 QLDKYNKDKENKLKLNDKDEYISFNFIEDK-LTESFHMNQIIHLINKKCVFTKCLENYKN 251
           +L+++ + K +  KL++    I+      K L E +   Q                    
Sbjct: 118 KLEQHPRYKRDNKKLSETLNIINLFPAYCKALNELYPFIQTSQEN--------------- 162

Query: 252 RYFVLKKEEILKKKKKQKKMSIFSYIVSIILFFTYII----SLINSCLYYIICTPKLFSE 307
               L+   +           I+  +  I  + T++I    SL++S L Y     +L + 
Sbjct: 163 ----LRGTMLNSVAAWCSSTCIYKMVAKIGFYLTFVICSIASLVSSLLNY--SHFQLVNY 216

Query: 308 YIFSKKCDGYLQNSAYPKFIFPS--EWHNI-FRSFMKNKQN----------PSEYYKYRE 354
             F ++ D   Q   Y    +    +  NI     M  K N          PS++Y    
Sbjct: 217 SAFVQQIDLRCQQICYFPVQYERINKKDNIQNVGSMVEKDNSNSQFSHSYMPSKFYPDYI 276

Query: 355 ILLIRIINLIIDIFLGFLIFLLLYFNVINLHYISEKAQIFYGTSTLTSILGTLLQNPLGF 414
           +L   I  +I DI  G ++  +L  N   L   S +   F+   +L +I  TL  NPLG 
Sbjct: 277 LLYNTIWLIINDISFGLILGAILIENRDFLVSASHRVLKFFLYDSLKTITETLANNPLGI 336

Query: 415 KLNNNFTSFIGSILVSILD-KWDLFTNTI--PVNNSTVLNFVGYTSLL-GFSFFLSFVID 470
           KLN    +F+  + + +++  +  F   +  P   S++L    Y   L GFSF +S  ID
Sbjct: 337 KLNAELANFLSELFLWVIEFSYTTFIKRLIDPKTLSSLLTLTIYMMFLVGFSFAVSLAID 396

Query: 471 YLRFVTAHVTIIYLFLKKICTLFHKNMYSLYLLFNGKKWNILKLRVDTNYYSNEEVLLGT 530
           +   ++  + + Y    K+       M SL+ LF GKK N+L+ R+D NY+  +++LLGT
Sbjct: 397 FFAILSFPIYVFYRISSKLYHCQLNIMASLFNLFCGKKRNVLRNRIDHNYFQLDQLLLGT 456

Query: 531 ILFTILIFLYPTIFVLVLVFGLIYLIINRIIYLLCVMEKIILYTPFYIFFIQPNCNKYIS 590
           +LF IL+FL PT+    + + ++ ++   I      +  +I + P +   ++    K + 
Sbjct: 457 LLFIILVFLTPTVMAFYMSYTVLRMLTITIEIFSEAVIALINHFPLFALLLRLKDPKRLP 516

Query: 591 KGFKFT 596
            G    
Sbjct: 517 GGISIE 522
>gb|AAC79574.1| (AF030178) N-acetylglucosaminyl transferase component Gpi1 [Mus
           musculus]
          Length = 581

 Score =  420 bits (1070), Expect = e-116
 Identities = 77/321 (23%), Positives = 143/321 (43%), Gaps = 14/321 (4%)

Query: 280 IILFFTYIISLINSCLYYIICTPKLFSEYIFSKKCDGYLQNSAYPKFIFPSEWHNIFRSF 339
           + L    ++SLI++     +   KL        K     +   +         H  F   
Sbjct: 212 VCLLLASLLSLISAASACRL--WKLGPLAFIRSKLS-TCEQLQH------RLKHLSFIFS 262

Query: 340 MKNKQNPSEYYKYREILLIRIINLIIDIFLGFLIFLLLYFNVINLHYISEKAQIF-YGTS 398
            +  Q+P +  +   +L+     +++D+ LG L+   L+ N       +    +      
Sbjct: 263 TEKAQSPMQLMRKANMLVS----VLLDVALGLLLLSWLHSNNRIGQLANALVPVADRVAE 318

Query: 399 TLTSILGTLLQNPLGFKLNNNFTSFIGSILVSILDKWDLFTNTIPVNNSTVLNFVGYTSL 458
            L  +L  L+  P G K+N      +G   +  +  W  + + +      +L  VG ++ 
Sbjct: 319 ELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIHLMSPFIEHILWHVGLSAC 378

Query: 459 LGFSFFLSFVIDYLRFVTAHVTIIYLFLKKICTLFHKNMYSLYLLFNGKKWNILKLRVDT 518
           LG +  LS   D +  +T H+   Y++  ++  L    + SL+ LF GKKWN+L+ RVD+
Sbjct: 379 LGLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIYGLSSLWRLFRGKKWNVLRQRVDS 438

Query: 519 NYYSNEEVLLGTILFTILIFLYPTIFVLVLVFGLIYLIINRIIYLLCVMEKIILYTPFYI 578
             Y  +++ +GT+LFTIL+FL PT  +  LVF L+ L++  +  L+ ++  +I   P Y 
Sbjct: 439 CSYDLDQLFIGTLLFTILVFLLPTTALYYLVFTLLRLLVITVQGLIHLLVDLINSLPLYS 498

Query: 579 FFIQPNCNKYISKGFKFTKYE 599
             ++      ++ G KF   E
Sbjct: 499 LGLRLCRPYRLAAGVKFRVLE 519
>gb|AAH03917.1|AAH03917 (BC003917) Unknown (protein for IMAGE:3485029) [Mus musculus]
          Length = 339

 Score =  396 bits (1008), Expect = e-109
 Identities = 71/284 (25%), Positives = 130/284 (45%), Gaps = 11/284 (3%)

Query: 317 YLQNSAYPKFIFPSEWHNIFRSFMKNKQNPSEYYKYREILLIRIINLIIDIFLGFLIFLL 376
             +   +         H  F    +  QNP +  +   +L+     +++D+ LG L+   
Sbjct: 4   TCEQLHH------RLKHLSFIFSTEKAQNPMQLMRKANMLVS----VLLDVALGLLLLSW 53

Query: 377 LYFNVINLHYISEKAQIF-YGTSTLTSILGTLLQNPLGFKLNNNFTSFIGSILVSILDKW 435
           L+ N       +    +       L  +L  L+  P G K+N      +G   +  +  W
Sbjct: 54  LHSNNRIGQLANALVPVADRVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLW 113

Query: 436 DLFTNTIPVNNSTVLNFVGYTSLLGFSFFLSFVIDYLRFVTAHVTIIYLFLKKICTLFHK 495
             + + +      +L  VG ++ LG +  LS   D +  +T H+   Y++  ++  L   
Sbjct: 114 ISYIHLMSPFIEHILWHVGLSACLGLTVALSIFSDIIALLTFHIYCFYVYGARLYCLKIY 173

Query: 496 NMYSLYLLFNGKKWNILKLRVDTNYYSNEEVLLGTILFTILIFLYPTIFVLVLVFGLIYL 555
            + SL+ LF GKKWN+L+ RVD+  Y  +++ +GT+LFTIL+FL PT  +  LVF L+ L
Sbjct: 174 GLSSLWRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILVFLLPTTALYYLVFTLLRL 233

Query: 556 IINRIIYLLCVMEKIILYTPFYIFFIQPNCNKYISKGFKFTKYE 599
           ++  +  L+ ++  +I   P Y   ++      ++ G KF   E
Sbjct: 234 LVITVQGLIHLLVDLINSLPLYSLGLRLCRPYRLAAGVKFRVLE 277
>emb|CAC10057.1| (AJ298996) GPI1 protein [Drosophila virilis]
          Length = 473

 Score =  345 bits (876), Expect = 2e-93
 Identities = 67/290 (23%), Positives = 127/290 (43%), Gaps = 10/290 (3%)

Query: 306 SEYIFSKKCDGYLQNSAYPKFIFPS--EWHNIFRSFMKNKQNPSEYYKYREILLIRIINL 363
             + +  +    L N     F +    ++ N   +     ++  E+         R+ N+
Sbjct: 157 DLWFYGSQSLCLLANIPMQMFQYIVGNKFINSIITHTVIYRHYKEWQSIYTKG-SRLSNI 215

Query: 364 IIDIFLGFLIFLLLYFNVINLHYISEKAQIFYGTSTLTSILGTLLQNPLGFKLNNNFTSF 423
           +ID  LG +I +L+ F +++                L S+L  L  +P+G KLN +  +F
Sbjct: 216 MIDRALG-IILMLVLFTLVSHPGDFLIQISHIIIHQLYSLLKVLEGSPIGLKLNIHLNNF 274

Query: 424 IGSILVSILDKWDLFTNTIPVNNSTVLNFVGYTSLLGFSFFLSFVIDYLRFVTAHVTIIY 483
                   ++ W  F + I      V   +G    LGF++ ++ + D +  V  H    Y
Sbjct: 275 FLDCFKYHIELWSTFLDLIEPIVRQVFLAIGAFGCLGFTYQIALLADLISIVGLHAHCFY 334

Query: 484 LFLKKICTLFHKNMYSLYLLFNGKKWNILKLRVDTNYYSNEEVLLGTILFTILIFLYPTI 543
           ++ K +  +  K +  L+ +  G ++NIL+ R++ + Y N ++ L TI F+ ++FL+PT 
Sbjct: 335 VYTKVLNNVEVKGLTVLWQVVRGNRYNILRNRIEAHNYMNRQLYLATIFFSAILFLFPTT 394

Query: 544 FVLVLVFGLIYLIINRIIYLLCVMEKIILYTPFYIFFIQPNCNKYISKGF 593
            V  +VF  +  +    +  L    + +L  P  +F       KYI KGF
Sbjct: 395 LVYYVVFATLKALTCATLATLECFRRKLLNFPIEMFL------KYIKKGF 438
>emb|CAC10056.1| (AJ298995) GPI1 protein [Drosophila melanogaster]
          Length = 481

 Score =  327 bits (831), Expect = 3e-88
 Identities = 57/265 (21%), Positives = 118/265 (44%), Gaps = 4/265 (1%)

Query: 324 PKFIFPSEWHNIFRSFMKNKQNPSEYYKYREILL---IRIINLIIDIFLGFLIFLLLYFN 380
           P  IF     N+F + +       +++K  +       R  N+++D  +  +I +L+ F+
Sbjct: 180 PIKIFEYMAENVFINSIMVHTTIYKHFKEWQTACDKRSRPANIVLDR-ILGIILMLILFS 238

Query: 381 VINLHYISEKAQIFYGTSTLTSILGTLLQNPLGFKLNNNFTSFIGSILVSILDKWDLFTN 440
           +             Y    L  +L  L  +P+G KLN +  +F        ++ W  F +
Sbjct: 239 LATQPGDFLIQISHYVIDELYGLLKVLEGSPIGLKLNIHLNNFFLDCFKYHIELWSTFLD 298

Query: 441 TIPVNNSTVLNFVGYTSLLGFSFFLSFVIDYLRFVTAHVTIIYLFLKKICTLFHKNMYSL 500
            I      V   +G    LGF+F ++ ++D +  +  H    Y++ K +  +  + +  L
Sbjct: 299 FIEPLVRQVFLAIGMIGCLGFTFQIALLVDLISVIGLHSHCFYIYTKVLYNVERRGLSVL 358

Query: 501 YLLFNGKKWNILKLRVDTNYYSNEEVLLGTILFTILIFLYPTIFVLVLVFGLIYLIINRI 560
           + +  G ++NILK R +++ Y N ++ L TI F+ ++FL PT  V  +VF  +  +    
Sbjct: 359 WQVVRGNRYNILKGRTESHNYMNRQLYLATIFFSAILFLLPTTLVYYIVFAALKALTFAT 418

Query: 561 IYLLCVMEKIILYTPFYIFFIQPNC 585
           + +   + + ++Y P  +   +   
Sbjct: 419 LSVFHFVRRKLMYLPIEVCIKRLLR 443
>ref|NP_502086.1| (NM_069685) F01G4.5.p [Caenorhabditis elegans]
 pir||T20486 hypothetical protein F01G4.5 - Caenorhabditis elegans
 emb|CAA92766.1| (Z68341) F01G4.5 [Caenorhabditis elegans]
          Length = 287

 Score =  230 bits (582), Expect = 6e-59
 Identities = 54/234 (23%), Positives = 83/234 (35%), Gaps = 27/234 (11%)

Query: 361 INLIIDIFLGFLIFLLL------YFNVINLHYISEKAQIFY-----GTSTLTSILGTLLQ 409
             +++     F   LLL      Y   I           F+         L   +  L  
Sbjct: 16  AKILLVRTFSFTRLLLLDVAFSIYLWNIWTPNWEWTVNEFWDQTGNVADNLNGTITWLRS 75

Query: 410 NPLGFKLNNNFTSFIGSILVSILDKWDLFTNTIPVN----------------NSTVLNFV 453
           NP G KLN      +       +  W                          +     F+
Sbjct: 76  NPAGLKLNTPVNETLAWFFTYHIYLWTSMLFCFQKFAFNFHNDFSAFIGFLRSDAFFRFI 135

Query: 454 GYTSLLGFSFFLSFVIDYLRFVTAHVTIIYLFLKKICTLFHKNMYSLYLLFNGKKWNILK 513
            Y+ + G S F + V D+ +    H      +  K+C L +  +  L+ L  GKKWN L+
Sbjct: 136 AYSLIGGISTFSAMVYDFSQIFFLHFNCFDAYATKLCYLCYYTLTVLWSLVRGKKWNPLR 195

Query: 514 LRVDTNYYSNEEVLLGTILFTILIFLYPTIFVLVLVFGLIYLIINRIIYLLCVM 567
            R DT      +  L T LF IL+F+ PTIFV  +VF  + L ++ +  +L   
Sbjct: 196 ERKDTVILDTRQQFLATSLFVILLFILPTIFVYFVVFRCLRLAVSALQTVLYFF 249
>pir||T45794 hypothetical protein F28O9.10 - Arabidopsis thaliana  (fragment)
 emb|CAB68122.1| (AL137080) hypothetical protein fragment [Arabidopsis thaliana]
          Length = 143

 Score =  156 bits (391), Expect = 1e-36
 Identities = 37/146 (25%), Positives = 69/146 (46%), Gaps = 7/146 (4%)

Query: 363 LIIDIFLGFLIFLLLYFNVINL-HYISEKAQIFYGTSTLTSILGTLLQNPLGFKLNNNFT 421
           + +D+ LG LI L L FN  ++  ++ + A+ F     L S    L+  P GFKLN    
Sbjct: 1   MAVDLVLGNLIGLGLLFNTESVCSFVFDFAKEF-TNGILRSGSVWLMGVPAGFKLNTELA 59

Query: 422 SFIGSILVSILDKWDL---FTNTIPVNNSTVLNFVGYTSLLGFSFFLSFVIDYLRFVTAH 478
             +G + ++++  W     F  +       V+  +G T   G +   +FVID + F T H
Sbjct: 60  GVLGMVSLNVIQIWSTLWVFMASFIFCLIRVIAILGIT--FGATVSAAFVIDVITFATLH 117

Query: 479 VTIIYLFLKKICTLFHKNMYSLYLLF 504
           +  ++  +  + +   + + +L+ LF
Sbjct: 118 IMALHWAITLVYSHQIQALAALWRLF 143
>dbj|BAB30464.1| (AK016852) data source:MGD, source key:MGI:1333114,
           evidence:ISS~phosphatidylinositol glycan, class
           Q~putative [Mus musculus]
          Length = 378

 Score = 81.0 bits (198), Expect = 5e-14
 Identities = 19/117 (16%), Positives = 41/117 (34%), Gaps = 13/117 (11%)

Query: 280 IILFFTYIISLINSCLYYIICTPKLFSEYIFSKKCDGYLQNSAYPKFIFPSEWHNIFRSF 339
           + L    ++SLI++     +   KL+       K     +   +         H  F   
Sbjct: 212 VCLLLASLLSLISAASACRL--WKLWPLSFIRSKLS-TCEQLHH------RLKHLSFIFS 262

Query: 340 MKNKQNPSEYYKYREILLIRIINLIIDIFLGFLIFLLLYFNVINLHYISEKAQIFYG 396
            +  QNP +  +   +L+     +++D+ LG L+   L+ N       +    +   
Sbjct: 263 TEKAQNPMQLMRKANMLVS----VLLDVALGLLLLSWLHSNNRIGQLANALVPVADV 315
>emb|CAB68123.2| (AL137080) hypothetical protein [Arabidopsis thaliana]
          Length = 261

 Score = 80.2 bits (196), Expect = 1e-13
 Identities = 8/73 (10%), Positives = 21/73 (27%), Gaps = 4/73 (5%)

Query: 204 KLKLNDKDEYISFNFIEDKLTESFHMNQIIHLINKKCVFTKCLENYKNRYFVLKKEEILK 263
            L  ++          + K  +  H  + ++ +    +   C    K  Y  +      +
Sbjct: 193 SLSFSNSSPQTKAPLKKPKWVDDLHNRKPLNEMETVILSLNCAAAAKIAYKKIS----TQ 248

Query: 264 KKKKQKKMSIFSY 276
            +   +  SI   
Sbjct: 249 LETSSQNFSISYL 261
>pir||T45795 hypothetical protein F28O9.20 - Arabidopsis thaliana
          Length = 242

 Score = 77.5 bits (189), Expect = 7e-13
 Identities = 6/48 (12%), Positives = 15/48 (30%)

Query: 204 KLKLNDKDEYISFNFIEDKLTESFHMNQIIHLINKKCVFTKCLENYKN 251
            L  ++          + K  +  H  + ++ +    +   C    KN
Sbjct: 193 SLSFSNSSPQTKAPLKKPKWVDDLHNRKPLNEMETVILSLNCAAAAKN 240
>gb|AAH10094.1|AAH10094 (BC010094) Similar to phosphatidylinositol glycan, class Q [Homo
           sapiens]
          Length = 299

 Score = 64.3 bits (155), Expect = 6e-09
 Identities = 10/72 (13%), Positives = 23/72 (31%), Gaps = 9/72 (12%)

Query: 280 IILFFTYIISLINSCLYYIICTPKLFSEYIFSKKCDGYLQNSAYPKFIFPSEWHNIFRSF 339
           I L    ++SL+++         KL+       K     +   +         H      
Sbjct: 212 ICLLLASLLSLVSAVSACR--VFKLWPLSFLGSKLS-TCEQLRH------RLEHLTLIFS 262

Query: 340 MKNKQNPSEYYK 351
            +  +NP++  +
Sbjct: 263 TRKAENPAQLMR 274
CPU time:   238.43 user secs.	    2.80 sys. secs	  241.23 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.317    0.161    0.457 

Gapped
Lambda     K      H
   0.270   0.0566    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 424159387
Number of Sequences: 887402
Number of extensions: 22671258
Number of successful extensions: 179179
Number of sequences better than 10.0: 2399
Number of HSP's better than 10.0 without gapping: 380
Number of HSP's successfully gapped in prelim test: 2141
Number of HSP's that attempted gapping in prelim test: 134177
Number of HSP's gapped (non-prelim): 12720
length of query: 669
length of database: 277,845,442
effective HSP length: 56
effective length of query: 613
effective length of database: 228,150,930
effective search space: 139856520090
effective search space used: 139856520090
T: 11
A: 40
X1: 15 ( 6.9 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (20.9 bits)
S2: 76 (33.7 bits)