PIG-P: AAF56563.1 BLAST Search Results

GPI Biosynthesis Report

GPI Anchor Biosynthesis Report: AAF56563.1 (PIG-P family, Drosophila melanogaster)

BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 
         (138 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!

 Results of PSI-Blast iteration 1

Distribution of 12 Blast Hits on the Query Sequence

Sequences with E-value BETTER than threshold (0.002)

Sequences producing significant alignments:


 gb|AAF56563.1|  (AE003754) CG14550 gene product [Drosophila melan...   247  4e-65

Sequences with E-value WORSE than threshold 

 dbj|BAB56104.1|  (AB028932) GLP1 [Homo sapiens]                         36  0.25

 gb|AAH11608.1|AAH11608  (BC011608) Unknown (protein for IMAGE:316...    36  0.29

 dbj|BAB47505.1|  (AB058779) KIAA1876 protein [Homo sapiens]             36  0.29

 sp|P57054|DSR5_HUMAN  Down syndrome critical region protein 5 (Do...    34  0.83

 pir||JC7301  Down syndrome critical region protein 5 alpha - huma...    34  0.90

 dbj|BAA95636.1|  (AB035745) DSCR5b [Homo sapiens] >gi|9963906|gb|...    34  0.95

 pir||JC7302  Down syndrome critical region protein 5 beta - human       33  1.9

 ref|NP_062416.1|  (NM_019543) Down syndrome critical region prote...    33  2.9

 ref|NP_347063.1|  (NC_003030) Fusion: PTS system, beta-glucosides...    33  3.2

 dbj|BAA95512.1|  (AP001727) Down syndrome critical region protein...    33  3.4

 ref|NP_057514.1|  (NM_016430) Down syndrome critical region prote...    33  3.7

Alignments
>gb|AAF56563.1| (AE003754) CG14550 gene product [Drosophila melanogaster]
          Length = 138

 Score =  247 bits (626), Expect = 4e-65
 Identities = 138/138 (100%), Positives = 138/138 (100%)

Query: 1   MLFTVLFLVYVTWALLPVEFGLHSYLPDKYFAVFVPFLVLVFAWFFAFLIYPAINLSMTV 60
           MLFTVLFLVYVTWALLPVEFGLHSYLPDKYFAVFVPFLVLVFAWFFAFLIYPAINLSMTV
Sbjct: 1   MLFTVLFLVYVTWALLPVEFGLHSYLPDKYFAVFVPFLVLVFAWFFAFLIYPAINLSMTV 60

Query: 61  DVDSIASVVDPKLALPKGTEFTSWSQLQKGKVSQDSASKKASPVNCNLCRTFHQPVTRAP 120
           DVDSIASVVDPKLALPKGTEFTSWSQLQKGKVSQDSASKKASPVNCNLCRTFHQPVTRAP
Sbjct: 61  DVDSIASVVDPKLALPKGTEFTSWSQLQKGKVSQDSASKKASPVNCNLCRTFHQPVTRAP 120

Query: 121 IPPLRFLDLQEVNTAYYN 138
           IPPLRFLDLQEVNTAYYN
Sbjct: 121 IPPLRFLDLQEVNTAYYN 138



>dbj|BAB56104.1| (AB028932) GLP1 [Homo sapiens]
          Length = 917

 Score = 36.4 bits (83), Expect = 0.25
 Identities = 19/53 (35%), Positives = 26/53 (48%)

Query: 84  WSQLQKGKVSQDSASKKASPVNCNLCRTFHQPVTRAPIPPLRFLDLQEVNTAY 136
           WS LQ  K  QDSA  + SPV   + R   +   R PIP +  +D +   + Y
Sbjct: 605 WSALQMSKALQDSAPDRPSPVERIVSRDIARGYERIPIPCVNAVDSEPCPSNY 657



>gb|AAH11608.1|AAH11608 (BC011608) Unknown (protein for IMAGE:3161748) [Homo sapiens]
          Length = 719

 Score = 36.0 bits (82), Expect = 0.29
 Identities = 19/53 (35%), Positives = 26/53 (48%)

Query: 84  WSQLQKGKVSQDSASKKASPVNCNLCRTFHQPVTRAPIPPLRFLDLQEVNTAY 136
           WS LQ  K  QDSA  + SPV   + R   +   R PIP +  +D +   + Y
Sbjct: 407 WSALQMSKALQDSAPDRPSPVERIVSRDIARGYERIPIPCVNAVDSEPCPSNY 459



>dbj|BAB47505.1| (AB058779) KIAA1876 protein [Homo sapiens]
          Length = 552

 Score = 36.0 bits (82), Expect = 0.29
 Identities = 19/53 (35%), Positives = 26/53 (48%)

Query: 84  WSQLQKGKVSQDSASKKASPVNCNLCRTFHQPVTRAPIPPLRFLDLQEVNTAY 136
           WS LQ  K  QDSA  + SPV   + R   +   R PIP +  +D +   + Y
Sbjct: 354 WSALQMSKALQDSAPDRPSPVERIVSRDIARGYERIPIPCVNAVDSEPCPSNY 406



>sp|P57054|DSR5_HUMAN Down syndrome critical region protein 5 (Down syndrome critical
           region protein C)
 dbj|BAA96872.1| (AB037163) DSCR5b [Homo sapiens]
          Length = 158

 Score = 34.5 bits (78), Expect = 0.83
 Identities = 23/68 (33%), Positives = 40/68 (58%), Gaps = 6/68 (8%)

Query: 7   FLVYVTWALLPVEF----GLHSYLPDKYFAVFVPFLVLVFAWFFAFLIYPAINLSMTVDV 62
           F++Y+ WA +P  +    GL +Y P KY+AV +P  +L+ A    +++   IN+  T  +
Sbjct: 51  FILYLVWAFIPESWLNSLGL-TYWPQKYWAVALPVYLLI-AIVIGYVLLFGINMMSTSPL 108

Query: 63  DSIASVVD 70
           DSI ++ D
Sbjct: 109 DSIHTITD 116



>pir||JC7301 Down syndrome critical region protein 5 alpha - human
 dbj|BAA95633.1| (AB035742) DSCR5a1 [Homo sapiens]
 dbj|BAA95634.1| (AB035743) DSCR5a2 [Homo sapiens]
 dbj|BAA95635.1| (AB035744) DSCR5a3 [Homo sapiens]
 dbj|BAA96871.1| (AB037162) DSCR5a [Homo sapiens]
 dbj|BAB12395.1| (AB039659) PIG-P [Homo sapiens]
 gb|AAH05180.1|AAH05180 (BC005180) Unknown (protein for MGC:12168) [Homo sapiens]
 gb|AAH11007.1|AAH11007 (BC011007) Unknown (protein for MGC:13327) [Homo sapiens]
          Length = 134

 Score = 34.5 bits (78), Expect = 0.90
 Identities = 23/68 (33%), Positives = 40/68 (58%), Gaps = 6/68 (8%)

Query: 7  FLVYVTWALLPVEF----GLHSYLPDKYFAVFVPFLVLVFAWFFAFLIYPAINLSMTVDV 62
          F++Y+ WA +P  +    GL +Y P KY+AV +P  +L+ A    +++   IN+  T  +
Sbjct: 27 FILYLVWAFIPESWLNSLGL-TYWPQKYWAVALPVYLLI-AIVIGYVLLFGINMMSTSPL 84

Query: 63 DSIASVVD 70
          DSI ++ D
Sbjct: 85 DSIHTITD 92



>dbj|BAA95636.1| (AB035745) DSCR5b [Homo sapiens]
 gb|AAG09757.1|AF237812_1 (AF237812) NPD010 [Homo sapiens]
          Length = 158

 Score = 34.5 bits (78), Expect = 0.95
 Identities = 23/68 (33%), Positives = 40/68 (58%), Gaps = 6/68 (8%)

Query: 7   FLVYVTWALLPVEF----GLHSYLPDKYFAVFVPFLVLVFAWFFAFLIYPAINLSMTVDV 62
           F++Y+ WA +P  +    GL +Y P KY+AV +P  +L+ A    +++   IN+  T  +
Sbjct: 51  FILYLVWAFIPESWLNSLGL-TYWPQKYWAVALPVYLLI-AIVIGYVLLFGINMMSTSPL 108

Query: 63  DSIASVVD 70
           DSI ++ D
Sbjct: 109 DSIHTITD 116



>pir||JC7302 Down syndrome critical region protein 5 beta - human
          Length = 158

 Score = 33.3 bits (75), Expect = 1.9
 Identities = 22/68 (32%), Positives = 40/68 (58%), Gaps = 6/68 (8%)

Query: 7   FLVYVTWALLPVEF----GLHSYLPDKYFAVFVPFLVLVFAWFFAFLIYPAINLSMTVDV 62
           F++Y+ WA +P  +    GL +Y P KY+AV +P  +L+ A    +++   +N+  T  +
Sbjct: 51  FILYLVWAFIPESWLNSLGL-TYWPQKYWAVALPVYLLI-AIVIGYVLLFGLNMMSTSPL 108

Query: 63  DSIASVVD 70
           DSI ++ D
Sbjct: 109 DSIHTITD 116



>ref|NP_062416.1| (NM_019543) Down syndrome critical region protein c [Mus
          musculus]
 gb|AAF32294.1|AF216306_1 (AF216306) DCRC-1 [Mus musculus]
 gb|AAF32295.1|AF216307_1 (AF216307) DCRC-2 [Mus musculus]
 gb|AAF32296.1|AF216308_1 (AF216308) DCRC-3 [Mus musculus]
 gb|AAF32297.1|AF216309_1 (AF216309) DCRC-4 [Mus musculus]
 dbj|BAB23204.1| (AK004169) Down syndrome critical region gene c~data source:MGD,
          source key:MGI:1860433, evidence:ISS~putative [Mus
          musculus]
          Length = 132

 Score = 32.9 bits (74), Expect = 2.9
 Identities = 22/68 (32%), Positives = 39/68 (57%), Gaps = 6/68 (8%)

Query: 7  FLVYVTWALLPVEF----GLHSYLPDKYFAVFVPFLVLVFAWFFAFLIYPAINLSMTVDV 62
          F++Y+ WA +P  +    GL +Y P KY+AV +P  +L+      +++   IN+  T  +
Sbjct: 27 FILYLVWAFVPESWLNSLGL-TYWPQKYWAVALPVYLLITV-VIGYVLLFGINMMSTSPL 84

Query: 63 DSIASVVD 70
          DSI ++ D
Sbjct: 85 DSIHTITD 92



>ref|NP_347063.1| (NC_003030) Fusion: PTS system, beta-glucosides specific IIABC
           component [Clostridium acetobutylicum]
 gb|AAF35839.1|AF205034_2 (AF205034) ScrA [Clostridium acetobutylicum]
 gb|AAK78403.1|AE007557_2 (AE007557) Fusion: PTS system, beta-glucosides specific IIABC
           component [Clostridium acetobutylicum]
          Length = 627

 Score = 32.5 bits (73), Expect = 3.2
 Identities = 15/47 (31%), Positives = 25/47 (52%)

Query: 4   TVLFLVYVTWALLPVEFGLHSYLPDKYFAVFVPFLVLVFAWFFAFLI 50
           TVL ++   W +  +E GL   +P+    +  PFL L+   FFA ++
Sbjct: 220 TVLPILISVWVMSYIEKGLRKIVPEALDILLTPFLTLMITGFFAMVV 266



>dbj|BAA95512.1| (AP001727) Down syndrome critical region protein C [Homo sapiens]
          Length = 108

 Score = 32.5 bits (73), Expect = 3.4
 Identities = 22/67 (32%), Positives = 39/67 (57%), Gaps = 6/67 (8%)

Query: 8  LVYVTWALLPVEF----GLHSYLPDKYFAVFVPFLVLVFAWFFAFLIYPAINLSMTVDVD 63
          ++Y+ WA +P  +    GL +Y P KY+AV +P  +L+ A    +++   IN+  T  +D
Sbjct: 2  VLYLVWAFIPESWLNSLGL-TYWPQKYWAVALPVYLLI-AIVIGYVLLFGINMMSTSPLD 59

Query: 64 SIASVVD 70
          SI ++ D
Sbjct: 60 SIHTITD 66



>ref|NP_057514.1| (NM_016430) Down syndrome critical region protein 5; Down
          syndrome critical region protein C short form; Down
          syndrome critical region protein C [Homo sapiens]
 gb|AAF32289.1|AF216305_1 (AF216305) Down syndrome critical region protein C short form
          [Homo sapiens]
 dbj|BAA96873.1| (AB037164) DSCR5c [Homo sapiens]
          Length = 108

 Score = 32.5 bits (73), Expect = 3.7
 Identities = 22/67 (32%), Positives = 39/67 (57%), Gaps = 6/67 (8%)

Query: 8  LVYVTWALLPVEF----GLHSYLPDKYFAVFVPFLVLVFAWFFAFLIYPAINLSMTVDVD 63
          ++Y+ WA +P  +    GL +Y P KY+AV +P  +L+ A    +++   IN+  T  +D
Sbjct: 2  VLYLVWAFIPESWLNSLGL-TYWPQKYWAVALPVYLLI-AVVIGYVLLFGINMMSTSPLD 59

Query: 64 SIASVVD 70
          SI ++ D
Sbjct: 60 SIHTITD 66



CPU time:    36.03 user secs.	    1.65 sys. secs	   37.68 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.327    0.140    0.441 

Gapped
Lambda     K      H
   0.270   0.0491    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 83502506
Number of Sequences: 887402
Number of extensions: 3101918
Number of successful extensions: 12204
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 18
Number of HSP's that attempted gapping in prelim test: 12194
Number of HSP's gapped (non-prelim): 23
length of query: 138
length of database: 277,845,442
effective HSP length: 49
effective length of query: 89
effective length of database: 234,362,744
effective search space: 20858284216
effective search space used: 20858284216
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (21.7 bits)
S2: 69 (31.2 bits)