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GPI Anchor Biosynthesis Report: T18769 (PIG-M family, Caenorhabditis elegans)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query=
         (417 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!


Results of PSI-Blast iteration 1

Distribution of 13 Blast Hits on the Query Sequence


Sequences with E-value BETTER than threshold (0.002)

ref|NP_496426.1| (NM_064025) B0491.1.p [Caenorhabditis elegans] ... 574 e-162
ref|NP_077058.1| (NM_024144) PIG-M mRNA for mannosyltransferase ... 501 e-140
ref|XP_049665.1| (XM_049665) similar to PIG-M mRNA for mannosylt... 500 e-140
dbj|BAB31275.1| (AK018560) data source:MGD, source key:MGI:19148... 500 e-140
gb|AAH05650.1|AAH05650 (BC005650) RIKEN cDNA 4933437L05 gene [Mu... 500 e-140
emb|CAB89880.1| (AL354632) hypothetical transmembrane protein [S... 457 e-127
ref|XP_080607.1| (XM_080607) CG9865 [Drosophila melanogaster] >g... 441 e-122
dbj|BAB20994.1| (AB050105) TbPIG-M [Trypanosoma brucei] 436 e-121
ref|NP_012547.1| (NC_001142) Protein required for cell viability... 317 3e-85
emb|CAC34506.1| (AL589883) mannosyltransferase-like protein [Ara... 306 7e-82
dbj|BAA87228.1| (AB027924) Hypothetical protein [Schizosaccharom... 161 2e-38
Sequences with E-value WORSE than threshold

ref|NP_536724.1| (NM_080476) CDC91 cell division cycle 91-like 1... 39 0.13
Alignments
>ref|NP_496426.1| (NM_064025) B0491.1.p [Caenorhabditis elegans]
 pir||T18769 hypothetical protein B0491.1 - Caenorhabditis elegans
 emb|CAA90090.1| (Z49907) B0491.1 [Caenorhabditis elegans]
          Length = 417

 Score =  574 bits (1465), Expect = e-162
 Identities = 417/417 (100%), Positives = 417/417 (100%)

Query: 1   MQCVRSFVKNETFNRNKILLVAFVARIILVFYAHIHDYLFKVNFTDIDYHVFSDAAKHVS 60
           MQCVRSFVKNETFNRNKILLVAFVARIILVFYAHIHDYLFKVNFTDIDYHVFSDAAKHVS
Sbjct: 1   MQCVRSFVKNETFNRNKILLVAFVARIILVFYAHIHDYLFKVNFTDIDYHVFSDAAKHVS 60

Query: 61  NGGSPFDRATYRYTPALAWILLPVVHFPDFGKILFCIFDILVAILYFKIMEKDLNETKSE 120
           NGGSPFDRATYRYTPALAWILLPVVHFPDFGKILFCIFDILVAILYFKIMEKDLNETKSE
Sbjct: 61  NGGSPFDRATYRYTPALAWILLPVVHFPDFGKILFCIFDILVAILYFKIMEKDLNETKSE 120

Query: 121 TREEMKDDQTMNVVIYWLANPLTAIISARGNAESIVAAVVLLNIVLLQKGYWKSAALVHG 180
           TREEMKDDQTMNVVIYWLANPLTAIISARGNAESIVAAVVLLNIVLLQKGYWKSAALVHG
Sbjct: 121 TREEMKDDQTMNVVIYWLANPLTAIISARGNAESIVAAVVLLNIVLLQKGYWKSAALVHG 180

Query: 181 ALAIQLKIYPLIYLPSVFLSLSTIGEQSCVVNKFKSLVSNWKGFAYMLVTLTSFAAVVLF 240
           ALAIQLKIYPLIYLPSVFLSLSTIGEQSCVVNKFKSLVSNWKGFAYMLVTLTSFAAVVLF
Sbjct: 181 ALAIQLKIYPLIYLPSVFLSLSTIGEQSCVVNKFKSLVSNWKGFAYMLVTLTSFAAVVLF 240

Query: 241 FFQIYGQLFLDEYLIYHVKRRDLAHNFSPYFYLLYLYEANPTMSQIIGLGAFIPQIVLIV 300
           FFQIYGQLFLDEYLIYHVKRRDLAHNFSPYFYLLYLYEANPTMSQIIGLGAFIPQIVLIV
Sbjct: 241 FFQIYGQLFLDEYLIYHVKRRDLAHNFSPYFYLLYLYEANPTMSQIIGLGAFIPQIVLIV 300

Query: 301 FFAFKHYDDLPFCWFITTFAFVTYNKVCTSQYFVWYIVLLPLLAHKIMMSRQLALSLMAA 360
           FFAFKHYDDLPFCWFITTFAFVTYNKVCTSQYFVWYIVLLPLLAHKIMMSRQLALSLMAA
Sbjct: 301 FFAFKHYDDLPFCWFITTFAFVTYNKVCTSQYFVWYIVLLPLLAHKIMMSRQLALSLMAA 360

Query: 361 WFATQGIWLLAAYLFEFQGWNTFFLMFLASCLFLIANSFILKQIINHYVPIVKPKTD 417
           WFATQGIWLLAAYLFEFQGWNTFFLMFLASCLFLIANSFILKQIINHYVPIVKPKTD
Sbjct: 361 WFATQGIWLLAAYLFEFQGWNTFFLMFLASCLFLIANSFILKQIINHYVPIVKPKTD 417
>ref|NP_077058.1| (NM_024144) PIG-M mRNA for mannosyltransferase [Rattus norvegicus]
 dbj|BAB18566.1| (AB028126) mannosyltransferase [Rattus norvegicus]
          Length = 423

 Score =  501 bits (1277), Expect = e-140
 Identities = 167/401 (41%), Positives = 237/401 (58%), Gaps = 26/401 (6%)

Query: 21  VAFVARIILVFYAHIHDYLFKVNFTDIDYHVFSDAAKHVSNGGSPFDRATYRYTPALAWI 80
           VAF+AR+ LVFY    D    V +TDIDYHVF+DAA+ V+ G SP+ RATYRYTP L+W+
Sbjct: 24  VAFLARVALVFYGVFQDRTLLVRYTDIDYHVFTDAARFVTEGRSPYLRATYRYTPLLSWL 83

Query: 81  LLPVVHFPD-FGKILFCIFDILVAILYFKIMEKDLNETKSETREEMKDDQTMNVVIYWLA 139
           L P V+  + FGK LF   D+L A              +    + +   Q     ++WL 
Sbjct: 84  LTPNVYLSELFGKFLFISCDLLTA----------FLLYRLLLLKGLGRRQACGYCVFWLL 133

Query: 140 NPLTAIISARGNAESIVAAVVLLNIVLLQKGYWKSAALVHGALAIQLKIYPLIYLPSVFL 199
           NPL   +S+RGNA+SIVA++VL  + L++K     AA+ +G  A+ +K+YP+ Y+  + L
Sbjct: 134 NPLPMAVSSRGNADSIVASLVLTTLYLIEKRLIACAAVFYG-FAVHMKMYPVTYILPIAL 192

Query: 200 S------------LSTIGEQSCVVNKFKSLVSNWKGFAYMLVTLTSFAAVVLFFFQIYGQ 247
                        L+    Q+ + +  K L S W    ++ +   +F A+   F+  YG 
Sbjct: 193 HLRPERDSDEGLRLARYSFQARLYDFLKRLCS-WAVLLFVAIAGLTFLALSFGFYYKYGW 251

Query: 248 LFLDEYLIYHVKRRDLAHNFSPYFYLLYLYEANPTMSQIIGLGAFIPQIVLIVFFAFKHY 307
            FL+   +YH+ RRD+ HNFSPYFY+LYL  A    S  +G+ AF+PQ +L+   +F +Y
Sbjct: 252 EFLEHTYLYHLTRRDIRHNFSPYFYMLYL-TAESKWSFTLGIAAFLPQFILLSAASFAYY 310

Query: 308 DDLPFCWFITTFAFVTYNKVCTSQYFVWYIVLLPLLAHKIMMSRQLALSLMAAWFATQGI 367
            DL FC F+ T  FVT+NKVCTSQYF+WY+ LLPL+   + M  + A+ L+  WF  Q +
Sbjct: 311 RDLVFCCFLHTSIFVTFNKVCTSQYFLWYLCLLPLVMPLVRMPWKRAVVLLMLWFIGQAL 370

Query: 368 WLLAAYLFEFQGWNTFFLMFLASCLFLIANSFILKQIINHY 408
           WL  AY+ EFQG NTF  ++LA   FL+ N  IL QII+HY
Sbjct: 371 WLAPAYVLEFQGKNTFLFIWLAGLFFLLINCSILIQIISHY 411
>ref|XP_049665.1| (XM_049665) similar to PIG-M mRNA for mannosyltransferase [Homo
           sapiens]
 dbj|BAB18567.1| (AB028127) mannosyltransferase [Homo sapiens]
 gb|AAH19865.1|AAH19865 (BC019865) Unknown (protein for MGC:29896) [Homo sapiens]
          Length = 423

 Score =  500 bits (1276), Expect = e-140
 Identities = 167/401 (41%), Positives = 234/401 (57%), Gaps = 26/401 (6%)

Query: 21  VAFVARIILVFYAHIHDYLFKVNFTDIDYHVFSDAAKHVSNGGSPFDRATYRYTPALAWI 80
           VAF+AR+ LVFY    D    V +TDIDY VF+DAA+ V+ G SP+ RATYRYTP L W+
Sbjct: 24  VAFLARVALVFYGVFQDRTLHVRYTDIDYQVFTDAARFVTEGRSPYLRATYRYTPLLGWL 83

Query: 81  LLPVVHFPD-FGKILFCIFDILVAILYFKIMEKDLNETKSETREEMKDDQTMNVVIYWLA 139
           L P ++  + FGK LF   D+L A              +    + +   Q     ++WL 
Sbjct: 84  LTPNIYLSELFGKFLFISCDLLTA----------FLLYRLLLLKGLGRRQACGYCVFWLL 133

Query: 140 NPLTAIISARGNAESIVAAVVLLNIVLLQKGYWKSAALVHGALAIQLKIYPLIYLPSVFL 199
           NPL   +S+RGNA+SIVA++VL+ + L++K     AA+ +G  A+ +KIYP+ Y+  + L
Sbjct: 134 NPLPMAVSSRGNADSIVASLVLMVLYLIKKRLVACAAVFYG-FAVHMKIYPVTYILPITL 192

Query: 200 SLSTIG------------EQSCVVNKFKSLVSNWKGFAYMLVTLTSFAAVVLFFFQIYGQ 247
            L                 Q+C+    K L  N     ++ V   +F A+   F+  YG 
Sbjct: 193 HLLPDRDNDKSLRQFRYTFQACLYELLKRLC-NRAVLLFVAVAGLTFFALSFGFYYEYGW 251

Query: 248 LFLDEYLIYHVKRRDLAHNFSPYFYLLYLYEANPTMSQIIGLGAFIPQIVLIVFFAFKHY 307
            FL+    YH+ RRD+ HNFSPYFY+LYL  A    S  +G+ AF+PQ++L+   +F +Y
Sbjct: 252 EFLEHTYFYHLTRRDIRHNFSPYFYMLYL-TAESKWSFSLGIAAFLPQLILLSAVSFAYY 310

Query: 308 DDLPFCWFITTFAFVTYNKVCTSQYFVWYIVLLPLLAHKIMMSRQLALSLMAAWFATQGI 367
            DL FC F+ T  FVT+NKVCTSQYF+WY+ LLPL+   + M  + A+ L+  WF  Q +
Sbjct: 311 RDLVFCCFLHTSIFVTFNKVCTSQYFLWYLCLLPLVMPLVRMPWKRAVVLLMLWFIGQAM 370

Query: 368 WLLAAYLFEFQGWNTFFLMFLASCLFLIANSFILKQIINHY 408
           WL  AY+ EFQG NTF  ++LA   FL+ N  IL QII+HY
Sbjct: 371 WLAPAYVLEFQGKNTFLFIWLAGLFFLLINCSILIQIISHY 411
>dbj|BAB31275.1| (AK018560) data source:MGD, source key:MGI:1914806,
           evidence:ISS~phosphatidylinositol glycan, class
           M~putative [Mus musculus]
          Length = 423

 Score =  500 bits (1274), Expect = e-140
 Identities = 166/401 (41%), Positives = 235/401 (58%), Gaps = 26/401 (6%)

Query: 21  VAFVARIILVFYAHIHDYLFKVNFTDIDYHVFSDAAKHVSNGGSPFDRATYRYTPALAWI 80
           VAF+AR+ LVFY    D    V +TDIDYHVF+DAA+ V+ G SP+ RATYRYTP L+W+
Sbjct: 24  VAFLARVALVFYGVFQDRTLLVRYTDIDYHVFTDAARFVTEGRSPYLRATYRYTPLLSWL 83

Query: 81  LLPVVHFPD-FGKILFCIFDILVAILYFKIMEKDLNETKSETREEMKDDQTMNVVIYWLA 139
           L P V+  + FGK LF   D+L A              +    + +   Q     ++WL 
Sbjct: 84  LTPNVYLSELFGKFLFISCDLLTA----------FLLYRLLLLKGLGRRQACGYCVFWLL 133

Query: 140 NPLTAIISARGNAESIVAAVVLLNIVLLQKGYWKSAALVHGALAIQLKIYPLIYLPSVFL 199
           NPL   +S+RGNA+SIVA++VL  +  ++K     AA+ +G  A+ +K+YP+ Y+  + L
Sbjct: 134 NPLPMAVSSRGNADSIVASLVLSTLYFIEKRLIACAAVFYG-FAVHMKMYPVTYILPIAL 192

Query: 200 SL------------STIGEQSCVVNKFKSLVSNWKGFAYMLVTLTSFAAVVLFFFQIYGQ 247
            L            +    Q+ + +  + L S W    ++ V   +F A+   F+  YG 
Sbjct: 193 HLRPERDDDERLRQARFSFQARLYDFLRRLCS-WAVLLFVAVAGLTFVALSFGFYYKYGW 251

Query: 248 LFLDEYLIYHVKRRDLAHNFSPYFYLLYLYEANPTMSQIIGLGAFIPQIVLIVFFAFKHY 307
            FL+    YH+ RRD+ HNFSPYFY+LYL  A    S  +G+ AF+PQ +L+   +F +Y
Sbjct: 252 EFLEHTYFYHLTRRDIRHNFSPYFYMLYL-TAESKWSFTLGIAAFLPQFILLSAASFAYY 310

Query: 308 DDLPFCWFITTFAFVTYNKVCTSQYFVWYIVLLPLLAHKIMMSRQLALSLMAAWFATQGI 367
            DL FC F+ T  FVT+NKVCTSQYF+WY+ LLPL+   + M  + A+ L+  WF  Q +
Sbjct: 311 RDLVFCCFLHTSIFVTFNKVCTSQYFLWYLCLLPLVMPLVRMPWKRAVVLLLFWFIGQAL 370

Query: 368 WLLAAYLFEFQGWNTFFLMFLASCLFLIANSFILKQIINHY 408
           WL  AY+ EFQG NTF  ++LA   FL+ N  IL QII+HY
Sbjct: 371 WLAPAYVLEFQGKNTFLFIWLAGLFFLLINCSILIQIISHY 411
>gb|AAH05650.1|AAH05650 (BC005650) RIKEN cDNA 4933437L05 gene [Mus musculus]
          Length = 423

 Score =  500 bits (1274), Expect = e-140
 Identities = 166/401 (41%), Positives = 235/401 (58%), Gaps = 26/401 (6%)

Query: 21  VAFVARIILVFYAHIHDYLFKVNFTDIDYHVFSDAAKHVSNGGSPFDRATYRYTPALAWI 80
           VAF+AR+ LVFY    D    V +TDIDYHVF+DAA+ V+ G SP+ RATYRYTP L+W+
Sbjct: 24  VAFLARVALVFYGVFQDRTLLVRYTDIDYHVFTDAARFVTEGRSPYLRATYRYTPLLSWL 83

Query: 81  LLPVVHFPD-FGKILFCIFDILVAILYFKIMEKDLNETKSETREEMKDDQTMNVVIYWLA 139
           L P V+  + FGK LF   D+L A              +    + +   Q     ++WL 
Sbjct: 84  LTPNVYLSELFGKFLFISCDLLTA----------FLLYRLLLLKGLGRRQACGYCVFWLL 133

Query: 140 NPLTAIISARGNAESIVAAVVLLNIVLLQKGYWKSAALVHGALAIQLKIYPLIYLPSVFL 199
           NPL   +S+RGNA+SIVA++VL  +  ++K     AA+ +G  A+ +K+YP+ Y+  + L
Sbjct: 134 NPLPMAVSSRGNADSIVASLVLSTLYFIEKRLIACAAVFYG-FAVHMKMYPVTYILPIAL 192

Query: 200 SL------------STIGEQSCVVNKFKSLVSNWKGFAYMLVTLTSFAAVVLFFFQIYGQ 247
            L            +    Q+ + +  + L S W    ++ V   +F A+   F+  YG 
Sbjct: 193 HLRPERDDDERLRHARFSFQARLYDFLRRLCS-WAVLLFVAVAGLTFVALSFGFYYKYGW 251

Query: 248 LFLDEYLIYHVKRRDLAHNFSPYFYLLYLYEANPTMSQIIGLGAFIPQIVLIVFFAFKHY 307
            FL+    YH+ RRD+ HNFSPYFY+LYL  A    S  +G+ AF+PQ +L+   +F +Y
Sbjct: 252 EFLEHTYFYHLTRRDIRHNFSPYFYMLYL-TAESKWSFTLGIAAFLPQFILLSAASFAYY 310

Query: 308 DDLPFCWFITTFAFVTYNKVCTSQYFVWYIVLLPLLAHKIMMSRQLALSLMAAWFATQGI 367
            DL FC F+ T  FVT+NKVCTSQYF+WY+ LLPL+   + M  + A+ L+  WF  Q +
Sbjct: 311 RDLVFCCFLHTSIFVTFNKVCTSQYFLWYLCLLPLVMPLVRMPWKRAVVLLLFWFIGQAL 370

Query: 368 WLLAAYLFEFQGWNTFFLMFLASCLFLIANSFILKQIINHY 408
           WL  AY+ EFQG NTF  ++LA   FL+ N  IL QII+HY
Sbjct: 371 WLAPAYVLEFQGKNTFLFIWLAGLFFLLINCSILIQIISHY 411
>emb|CAB89880.1| (AL354632) hypothetical transmembrane protein [Schizosaccharomyces
           pombe]
          Length = 815

 Score =  457 bits (1165), Expect = e-127
 Identities = 141/391 (36%), Positives = 214/391 (54%), Gaps = 17/391 (4%)

Query: 18  ILLVAFVARIILVFYAHIHDYLFKVNFTDIDYHVFSDAAKHVSNGGSPFDRATYRYTPAL 77
           +LL+  + R++L+ Y   HD    + FTDIDY VFSDA+K+VS G SP+ R TYRYTP L
Sbjct: 438 LLLLGLLMRLVLLNYGIWHDRRSALKFTDIDYFVFSDASKYVSIGMSPYMRDTYRYTPML 497

Query: 78  AWILLPVVH-FPDFGKILFCIFDILVAILYFKIMEKDLNETKSETREEMKDDQTMNVVIY 136
           A +LLP  + FP +GK LF I D++   L  K++ + ++  +S                +
Sbjct: 498 AILLLPTQYGFPSWGKYLFSISDLIAGWLMIKLLSRRISYKRSLIYSS-----------F 546

Query: 137 WLANPLTAIISARGNAESIVAAVVLLNIVLLQKGYWKSAALVHGALAIQLKIYPLIYLPS 196
           W+ NP  AIIS RGN E+I+  + +  + L++K     A+L+ G  ++  KIYP +Y  +
Sbjct: 547 WILNPFVAIISTRGNCEAILGILSIALLYLIEKKSVWLASLILG-FSVHFKIYPFMYGIA 605

Query: 197 VFLSLSTIGEQSCVVNKFKSLVSNWKGFAYMLVTLTSFAAVVLFFFQIYGQLFLDEYLIY 256
             +  S   + S  + KF SL+S       ++ +L  F    L  + +YG  FL+   +Y
Sbjct: 606 FLVYFSKPKKGSTFMEKFLSLLS-INQLKIVVGSLFMFTICNLLMYYLYGSPFLEHTYLY 664

Query: 257 HVKRRDLAHNFSPYFYLLYLYEANPTMSQIIGLGAFIPQIVLIVFFAFKH-YDDLPFCWF 315
           H  R D  HNFS   + L LY  +   ++   L AF+PQ+ L +         +LP   F
Sbjct: 665 HFGRTDHRHNFS--LHHLNLYYESSFGAKASSLFAFLPQLSLCMLIPLVFGKKNLPGTLF 722

Query: 316 ITTFAFVTYNKVCTSQYFVWYIVLLPLLAHKIMMSRQLALSLMAAWFATQGIWLLAAYLF 375
             TFAFVT+NKVCTSQYF+WY+V LPL+     +  +  L  ++ W   Q +WL++AY  
Sbjct: 723 AQTFAFVTFNKVCTSQYFMWYLVFLPLVLPNSKLLSKKGLICLSLWIIGQLLWLISAYNL 782

Query: 376 EFQGWNTFFLMFLASCLFLIANSFILKQIIN 406
           E  G + F  ++L+  LF   N + LK I++
Sbjct: 783 EMLGKSVFIPLWLSGLLFFFFNVYELKIILD 813
>ref|XP_080607.1| (XM_080607) CG9865 [Drosophila melanogaster]
 gb|AAF46748.1| (AE003454) CG9865 gene product [Drosophila melanogaster]
 gb|AAK77251.1| (AY047519) GH02741p [Drosophila melanogaster]
          Length = 466

 Score =  441 bits (1124), Expect = e-122
 Identities = 148/428 (34%), Positives = 227/428 (52%), Gaps = 39/428 (9%)

Query: 19  LLVAFVARIILVFYAHIHDYLFKVNFTDIDYHVFSDAAKHVSNGGSPFDRATYRYTPALA 78
           LL++ + RI L+ Y  +HD    V +TDIDY V +D A+ V  G +PF R TYRY+P +A
Sbjct: 27  LLISALLRIALICYGQLHDSQSAVPYTDIDYKVVTDGARQVLAGDTPFARHTYRYSPIMA 86

Query: 79  WI-LLPVVHFPDFGKILFCIFDILVAILYFKIMEKDLNETKSETREEM--------KDDQ 129
           ++    ++  P +GK+L+  FD+L+A L ++++  ++     +T + +          DQ
Sbjct: 87  YLQTFNILLHPAWGKLLYATFDLLIATLIYRLVHMEIKSQYQKTVQHLLSKFNRPRDSDQ 146

Query: 130 TMNVV--------------IYWLANPLTAIISARGNAESIVAAVVLLNIVLLQKG----- 170
           +++ +               +WL NPLTA+IS RG+ +   +  V+L I LL K      
Sbjct: 147 SLDALDERSHPENLARASACFWLYNPLTAVISTRGSGDCFSSFFVILTIYLLLKSEHNVT 206

Query: 171 ----YWKSAALVHGALAIQLKIYPLIYLPSVFLSLSTIGEQSCVVNKFKSLVSNWKGFAY 226
                   A L HG L I L++YPL++  + +LSLST   Q+ +    + L  N +    
Sbjct: 207 RSYWLIFGAGLAHG-LVIHLRLYPLLFSLAYYLSLSTRLTQTPLDFLCQILRPNKQQLCL 265

Query: 227 MLVTLTSFAAVVLFFFQIYGQLFLDEYLIYHVKRRDLAHNFSPYFYLLYLYEANPTM--S 284
           +  TL S  A    F+ +YG  ++ E  +YH  R+D  HNFS  F L YL  A+     S
Sbjct: 266 ISGTLISLVAFTWTFYTMYGWEYIYEAYLYHFVRKDPRHNFSLQFLLQYLGSASSVAEPS 325

Query: 285 QIIGLGAFIPQIVLIVFFAF---KHYDDLPFCWFITTFAFVTYNKVCTSQYFVWYIVLLP 341
            I+      PQ +LI++ +    +    LPFC F   F  VTYN V TSQYF+WY+ +LP
Sbjct: 326 AILKTLVLAPQFLLILYLSLSFGQFRQTLPFCIFAVAFVIVTYNSVVTSQYFIWYLAILP 385

Query: 342 LLAHKI-MMSRQLALSLMAAWFATQGIWLLAAYLFEFQGWNTFFLMFLASCLFLIANSFI 400
           L  +   M+S +    L+  W   Q +WLL AYL EF  WNTF+ + L   +F   N +I
Sbjct: 386 LCLNNFKMLSWRRCFGLLFLWLLAQALWLLPAYLLEFHTWNTFYWIGLQGAVFFATNGYI 445

Query: 401 LKQIINHY 408
           L+Q+++HY
Sbjct: 446 LEQLLSHY 453
>dbj|BAB20994.1| (AB050105) TbPIG-M [Trypanosoma brucei]
          Length = 430

 Score =  436 bits (1111), Expect = e-121
 Identities = 139/410 (33%), Positives = 213/410 (51%), Gaps = 39/410 (9%)

Query: 11  ETFNRNKILLVAFVARIILVFYAHIHDYLFKVNFTDIDYHVFSDAAKHVSNGGSPFDRAT 70
           +T +  K+LL+  + R+IL+ YA  HD  F+V +TDIDY +  D A+H+ NGGSPFDR T
Sbjct: 8   DTVSLQKLLLLGALLRLILIAYAFFHDQWFRVKYTDIDYMIVVDGARHMWNGGSPFDRTT 67

Query: 71  YRYTPALAWILLPVVHFPD-FGKILFCIFDILVAILYFKIMEKDLNETKSETREEMKDDQ 129
           +RYTP LA +++P +   +  GK++F   D+  A   + +++    E  ++         
Sbjct: 68  FRYTPLLAALVMPSIWIANPMGKLIFASSDLGAAWYCYGVLKSFAKERSAK--------- 118

Query: 130 TMNVVIYWLANPLTAIISARGNAESIVAAVVLLNIVLLQKGYWKSAALVHGALAIQLKIY 189
              V ++ L NP+   +S RGN++ +V  + L+ +    +     AA V G  A+  KIY
Sbjct: 119 -WMVSLFILFNPIVLSVSTRGNSDMLVTFMSLMVLSKFARRKCYQAAAVLG-FAVHFKIY 176

Query: 190 PLIYLPSVFLSLSTIGEQSCV--VNKFKSLVSNWKGFAY-MLVTLTSFAAVVLFFFQIYG 246
           P+IY   + L    + EQS     N ++ +V      +   L+   SFA   +  +  YG
Sbjct: 177 PIIYALPLTLG---VWEQSVAASTNTWRRVVKTAVVVSICALMAAISFAVPTVLCYMKYG 233

Query: 247 QLFLDEYLIYHVKRRDLAHNFSPYFYLLYLYEANPTMSQIIG----LGAFIPQIVLIVFF 302
           Q +L+E  IYHV R D  HNFSPY+ L+YL  A   + Q +     L AF+PQ V++ F 
Sbjct: 234 QQYLNEAFIYHVYREDHRHNFSPYWLLMYLNMARRHLGQGVDFSPRLVAFVPQAVVLSFV 293

Query: 303 AFKHYDDLPFCWFITTFAFVTYNKVCTSQYFVWYIVLLPLL----------------AHK 346
           ++K   +      + T  FV +NKVCT QYFVW+I  L  L                A K
Sbjct: 294 SYKLRRNTAHACCVQTVLFVAFNKVCTVQYFVWFIPFLAFLFCEPKEVEDDESGGSGAFK 353

Query: 347 IMMSRQLALSLMAAWFATQGIWLLAAYLFEFQGWNTFFLMFLASCLFLIA 396
              S   AL ++  W AT  +W+  A   EF G++ F  +++ SCLF +A
Sbjct: 354 -FFSWVKALGVVLMWAATIPLWVTTAVPLEFHGYSDFAKLWIVSCLFFLA 402
>ref|NP_012547.1| (NC_001142) Protein required for cell viability; Yjr013wp
           [Saccharomyces cerevisiae]
 sp|P47088|YJY3_YEAST HYPOTHETICAL 35.6 KD PROTEIN IN SPC1-ILV3 INTERGENIC REGION
 pir||S55201 hypothetical protein YJR013w - yeast (Saccharomyces cerevisiae)
 emb|CAA60935.1| (X87611) ORF YJR83.11 [Saccharomyces cerevisiae]
 emb|CAA89537.1| (Z49513) ORF YJR013w [Saccharomyces cerevisiae]
          Length = 305

 Score =  317 bits (805), Expect = 3e-85
 Identities = 105/305 (34%), Positives = 162/305 (52%), Gaps = 25/305 (8%)

Query: 108 KIMEKDLNETKSETREEMKDDQTMNVVIYWLANPLTAIISARGNAESIVAAVVLLNIVLL 167
           K++ + ++  ++   E +           WL NP+   IS RGNAES++  +++  +  L
Sbjct: 2   KLLNQAISRKRALILESI-----------WLLNPMVITISTRGNAESVLCCLIMFTLFFL 50

Query: 168 QKGYWKSAALVHGALAIQLKIYPLIYLPSVFLSLSTIGEQSCVVNKFKSLVSNWKGFAYM 227
           QK  +  A +++G L+I  KIYP+IY   + + +           +  SL++        
Sbjct: 51  QKSRYTLAGILYG-LSIHFKIYPIIYCIPIAIFIYYNKRNQGPRTQLTSLLN------IG 103

Query: 228 LVTLTSFAAVVLFFFQIYGQLFLDEYLIYHVKRRDLAHNFSPYFYLLYLYEANPTMSQI- 286
           L TLT+        ++IYG  FLD+  +YH+ R D  HNFS +  LLYL  AN    +  
Sbjct: 104 LSTLTTLLGCGWAMYKIYGYEFLDQAYLYHLYRTDHRHNFSVWNMLLYLDSANKENGESN 163

Query: 287 IGLGAFIPQIVLIVFFAFKH-----YDDLPFCWFITTFAFVTYNKVCTSQYFVWYIVLLP 341
           +   AF+PQ++L++           +D+L    F+ TFAFVTYNKVCTSQYFVWY++ LP
Sbjct: 164 LSRYAFVPQLLLVLVTGCLEWWNPTFDNLLRVLFVQTFAFVTYNKVCTSQYFVWYLIFLP 223

Query: 342 LLAHKIMMSRQLALSLMAAWFATQGIWLLAAYLFEFQGWNTFFL-MFLASCLFLIANSFI 400
               +  +  +  L +   W  TQGIWL   Y  EF+G N F+  +F+AS LF + N ++
Sbjct: 224 FYLSRTHIGWKKGLLMATLWVGTQGIWLSQGYYLEFEGKNVFYPGLFIASVLFFVTNVWL 283

Query: 401 LKQII 405
           L Q I
Sbjct: 284 LGQFI 288
>emb|CAC34506.1| (AL589883) mannosyltransferase-like protein [Arabidopsis thaliana]
          Length = 417

 Score =  306 bits (776), Expect = 7e-82
 Identities = 102/299 (34%), Positives = 146/299 (48%), Gaps = 34/299 (11%)

Query: 142 LTAIISARGNAESIVAAVVLLNIVLLQKGYWKSAALVHGAL----AIQLKIYPLIYLPSV 197
            +A +    N   I  +VVL +I + Q  YW +  L+  A      +  ++YP+IY   +
Sbjct: 99  FSASVHQPHNVSMICFSVVLESISMFQI-YWSAGNLLQAAFWYGLVVHFRVYPIIYALPI 157

Query: 198 FLSLST----IGEQSCV----VNKFKSLVSNWKGFAYMLVTLTS---------------- 233
            L L T     G++  +      + K+  SN +   ++   LT+                
Sbjct: 158 ILVLDTQVFRSGQKPALLYWNTGQAKTPASNMERKTFLFNLLTTLKSLFSRERIMFALIS 217

Query: 234 ---FAAVVLFFFQIYGQLFLDEYLIYHVKRRDLAHNFSPYFYLLYLYEANPTMSQIIGLG 290
              F A     F  YGQ FL E L+YH+ R D  HNFS YFY +YL+      S +  L 
Sbjct: 218 GGVFLACNAVSFYFYGQEFLHEALLYHLTRTDPRHNFSIYFYHIYLHYER-QFSAVEKLI 276

Query: 291 AFIPQIVLIVFFAFKHYDDLPFCWFITTFAFVTYNKVCTSQYFVWYIVLLPL-LAHKIMM 349
           +F+PQ  +     F    DL FC F+ T AFVT+NKV T+QYFVW+  LLPL L    M 
Sbjct: 277 SFLPQFTVQFALVFCFSQDLVFCIFLQTVAFVTFNKVITAQYFVWFYCLLPLILPWSHMK 336

Query: 350 SRQLALSLMAAWFATQGIWLLAAYLFEFQGWNTFFLMFLASCLFLIANSFILKQIINHY 408
            +   L  +  W   Q  WLL  Y+ EF+G N F  +++AS LFL AN+F+L +II  +
Sbjct: 337 LKWEGLLCIIMWIGAQTHWLLWGYMLEFKGVNVFLPLWIASLLFLAANTFVLVRIIQRH 395
 Score =  112 bits (280), Expect = 9e-24
 Identities = 34/82 (41%), Positives = 50/82 (60%), Gaps = 1/82 (1%)

Query: 18  ILLVAFVARIILVFYAHIHDYLFKVNFTDIDYHVFSDAAKHVSNGGSPFDRATYRYTPAL 77
           +L+ + + R+ L+ Y    D   +V +TD+DY VFSDAA  +++G SP+ R TYRY+P L
Sbjct: 21  LLVFSMLLRVFLIVYGEWQDAHMEVRYTDVDYIVFSDAASLMASGESPYKRTTYRYSPLL 80

Query: 78  AWILLPVVHF-PDFGKILFCIF 98
           A +L P   F   +GK LF   
Sbjct: 81  ALLLTPNSFFHRSWGKFLFSAS 102
>dbj|BAA87228.1| (AB027924) Hypothetical protein [Schizosaccharomyces pombe]
          Length = 149

 Score =  161 bits (406), Expect = 2e-38
 Identities = 42/157 (26%), Positives = 74/157 (46%), Gaps = 13/157 (8%)

Query: 106 YFKIMEKDLNETKSETREEMKDDQTMNVVIYWLANPLTAIISARGNAESIVAAVVLLNIV 165
             K++ + ++  +S                +W+ NP  AIIS RGN E+I+  + +  + 
Sbjct: 6   MIKLLSRRISYKRSLIYSS-----------FWILNPFVAIISTRGNCEAILGILSIALLY 54

Query: 166 LLQKGYWKSAALVHGALAIQLKIYPLIYLPSVFLSLSTIGEQSCVVNKFKSLVSNWKGFA 225
           L++K     A+L+ G  ++  KIYP +Y  +  +  S   + S  + KF SL+S      
Sbjct: 55  LIEKKSVWLASLILG-FSVHFKIYPFMYGIAFLVYFSKPKKGSTFMEKFLSLLS-INQLK 112

Query: 226 YMLVTLTSFAAVVLFFFQIYGQLFLDEYLIYHVKRRD 262
            ++ +L  F    L  + +YG  FL+   +YH  R D
Sbjct: 113 IVVGSLFMFTICNLLMYYLYGSPFLEHTYLYHFGRTD 149
>ref|NP_536724.1| (NM_080476) CDC91 cell division cycle 91-like 1 [Homo sapiens]
 emb|CAC14080.1| (AL118520) bA346K17.2 (A novel protein similar to the cell division
           control protein 91 (CDC91, YLR459W or L9122.2) from
           Yeast) [Homo sapiens]
          Length = 435

 Score = 39.2 bits (91), Expect = 0.13
 Identities = 58/325 (17%), Positives = 107/325 (32%), Gaps = 40/325 (12%)

Query: 52  FSDAAKHVSNGGSPFDRATYRYTPALAWILLPVVHFPDFGKILFCIFDILVAILYFKIME 111
             +    +  G SP+  A +  TP + ++        D+ +++F I D L AI  +  ++
Sbjct: 44  VVEGLSLLDLGVSPYSGAVFHETPLIIYLFH---FLIDYAELVFMITDALTAIALYFAIQ 100

Query: 112 KDLN--ETKSETREEMK----------------DDQTMNVVIYWLANPLTA----IISAR 149
                   K +   E+                     + V +++L NP T       S  
Sbjct: 101 DFNKVVFKKQKLLLELDQYAPDVAELIRTPMEMRYIPLKVALFYLLNPYTILSCVAKSTC 160

Query: 150 GNAESIVAAVVLLNIVLLQKGYWKSAALVHGALAIQLKIYPLIYLPSVFLSLSTIGEQSC 209
               +++A  +L  I    KG    +A+   ALA    +YPL       L L        
Sbjct: 161 AINNTLIAFFILTTI----KGSAFLSAIFL-ALATYQSLYPLTLFVPGLLYLLQRQYIPV 215

Query: 210 VVNKFKSLVSNWKGFAYMLVTLTSFAAVVLFFFQIYGQLFLDEYLIYHVKRRDLAHNFSP 269
            +      + +W+     + +L     + L FF +    F+     + +   DL  N   
Sbjct: 216 KMKSKAFWIFSWEYAMMYVGSLV--VIICLSFFLLSSWDFIPAVYGFILSVPDLTPNIGL 273

Query: 270 YFYLLYLYEANPTMSQIIGLGAFIPQIVLIVF---FAFKHYDDLPFCWFITTFAFVTYNK 326
           ++Y    + A       +     + QI +  +    A K  +   F  FI       +  
Sbjct: 274 FWY----FFAEMFEHFSL-FFVCVFQINVFFYTIPLAIKLKEHPIFFMFIQIAVIAIFKS 328

Query: 327 VCTSQYFVWYIVLLPLLAHKIMMSR 351
             T      Y+   P+  H     R
Sbjct: 329 YPTVGDVALYMAFFPVWNHLYRFLR 353
CPU time:   136.42 user secs.	    2.32 sys. secs	  138.74 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.321    0.177    0.575 

Gapped
Lambda     K      H
   0.270   0.0622    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 328520622
Number of Sequences: 887402
Number of extensions: 18566658
Number of successful extensions: 63175
Number of sequences better than 10.0: 243
Number of HSP's better than 10.0 without gapping: 19
Number of HSP's successfully gapped in prelim test: 230
Number of HSP's that attempted gapping in prelim test: 62896
Number of HSP's gapped (non-prelim): 414
length of query: 417
length of database: 277,845,442
effective HSP length: 51
effective length of query: 366
effective length of database: 232,587,940
effective search space: 85127186040
effective search space used: 85127186040
T: 11
A: 40
X1: 15 ( 6.9 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (21.0 bits)
S2: 75 (33.2 bits)