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GPI Anchor Biosynthesis Report: BAB20994.1 (PIG-M family, Trypanosoma brucei)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query=
         (430 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!


Results of PSI-Blast iteration 1

Distribution of 13 Blast Hits on the Query Sequence


Sequences with E-value BETTER than threshold (0.002)

dbj|BAB20994.1| (AB050105) TbPIG-M [Trypanosoma brucei] 574 e-162
ref|NP_077058.1| (NM_024144) PIG-M mRNA for mannosyltransferase ... 486 e-136
dbj|BAB31275.1| (AK018560) data source:MGD, source key:MGI:19148... 485 e-136
gb|AAH05650.1|AAH05650 (BC005650) RIKEN cDNA 4933437L05 gene [Mu... 485 e-136
ref|XP_049665.1| (XM_049665) similar to PIG-M mRNA for mannosylt... 479 e-134
ref|NP_496426.1| (NM_064025) B0491.1.p [Caenorhabditis elegans] ... 452 e-126
emb|CAB89880.1| (AL354632) hypothetical transmembrane protein [S... 442 e-123
emb|CAC34506.1| (AL589883) mannosyltransferase-like protein [Ara... 371 e-102
ref|NP_012547.1| (NC_001142) Protein required for cell viability... 304 1e-81
ref|XP_080607.1| (XM_080607) CG9865 [Drosophila melanogaster] >g... 299 6e-80
dbj|BAA87228.1| (AB027924) Hypothetical protein [Schizosaccharom... 166 6e-40
Sequences with E-value WORSE than threshold

ref|NP_346994.1| (NC_003030) Uncharacterized membrane protein [C... 44 0.005
Alignments
>dbj|BAB20994.1| (AB050105) TbPIG-M [Trypanosoma brucei]
          Length = 430

 Score =  574 bits (1464), Expect = e-162
 Identities = 430/430 (100%), Positives = 430/430 (100%)

Query: 1   MELQSLIDTVSLQKLLLLGALLRLILIAYAFFHDQWFRVKYTDIDYMIVVDGARHMWNGG 60
           MELQSLIDTVSLQKLLLLGALLRLILIAYAFFHDQWFRVKYTDIDYMIVVDGARHMWNGG
Sbjct: 1   MELQSLIDTVSLQKLLLLGALLRLILIAYAFFHDQWFRVKYTDIDYMIVVDGARHMWNGG 60

Query: 61  SPFDRTTFRYTPLLAALVMPSIWIANPMGKLIFASSDLGAAWYCYGVLKSFAKERSAKWM 120
           SPFDRTTFRYTPLLAALVMPSIWIANPMGKLIFASSDLGAAWYCYGVLKSFAKERSAKWM
Sbjct: 61  SPFDRTTFRYTPLLAALVMPSIWIANPMGKLIFASSDLGAAWYCYGVLKSFAKERSAKWM 120

Query: 121 VSLFILFNPIVLSVSTRGNSDMLVTFMSLMVLSKFARRKCYQAAAVLGFAVHFKIYPIIY 180
           VSLFILFNPIVLSVSTRGNSDMLVTFMSLMVLSKFARRKCYQAAAVLGFAVHFKIYPIIY
Sbjct: 121 VSLFILFNPIVLSVSTRGNSDMLVTFMSLMVLSKFARRKCYQAAAVLGFAVHFKIYPIIY 180

Query: 181 ALPLTLGVWEQSVAASTNTWRRVVKTAVVVSICALMAAISFAVPTVLCYMKYGQQYLNEA 240
           ALPLTLGVWEQSVAASTNTWRRVVKTAVVVSICALMAAISFAVPTVLCYMKYGQQYLNEA
Sbjct: 181 ALPLTLGVWEQSVAASTNTWRRVVKTAVVVSICALMAAISFAVPTVLCYMKYGQQYLNEA 240

Query: 241 FIYHVYREDHRHNFSPYWLLMYLNMARRHLGQGVDFSPRLVAFVPQAVVLSFVSYKLRRN 300
           FIYHVYREDHRHNFSPYWLLMYLNMARRHLGQGVDFSPRLVAFVPQAVVLSFVSYKLRRN
Sbjct: 241 FIYHVYREDHRHNFSPYWLLMYLNMARRHLGQGVDFSPRLVAFVPQAVVLSFVSYKLRRN 300

Query: 301 TAHACCVQTVLFVAFNKVCTVQYFVWFIPFLAFLFCEPKEVEDDESGGSGAFKFFSWVKA 360
           TAHACCVQTVLFVAFNKVCTVQYFVWFIPFLAFLFCEPKEVEDDESGGSGAFKFFSWVKA
Sbjct: 301 TAHACCVQTVLFVAFNKVCTVQYFVWFIPFLAFLFCEPKEVEDDESGGSGAFKFFSWVKA 360

Query: 361 LGVVLMWAATIPLWVTTAVPLEFHGYSDFAKLWIVSCLFFLAMVVLASMLARIAYRVQCT 420
           LGVVLMWAATIPLWVTTAVPLEFHGYSDFAKLWIVSCLFFLAMVVLASMLARIAYRVQCT
Sbjct: 361 LGVVLMWAATIPLWVTTAVPLEFHGYSDFAKLWIVSCLFFLAMVVLASMLARIAYRVQCT 420

Query: 421 KCSAKSIKVA 430
           KCSAKSIKVA
Sbjct: 421 KCSAKSIKVA 430
>ref|NP_077058.1| (NM_024144) PIG-M mRNA for mannosyltransferase [Rattus norvegicus]
 dbj|BAB18566.1| (AB028126) mannosyltransferase [Rattus norvegicus]
          Length = 423

 Score =  486 bits (1239), Expect = e-136
 Identities = 129/399 (32%), Positives = 204/399 (50%), Gaps = 35/399 (8%)

Query: 18  LGALLRLILIAYAFFHDQWFRVKYTDIDYMIVVDGARHMWNGGSPFDRTTFRYTPLLAAL 77
           +  L R+ L+ Y  F D+   V+YTDIDY +  D AR +  G SP+ R T+RYTPLL+ L
Sbjct: 24  VAFLARVALVFYGVFQDRTLLVRYTDIDYHVFTDAARFVTEGRSPYLRATYRYTPLLSWL 83

Query: 78  VMPSIWIANPMGKLIFASSDLGAAWYCYGVLKSFAKERSAKWMVSLFILFNPIVLSVSTR 137
           + P+++++   GK +F S DL  A+  Y +L      R       +F L NP+ ++VS+R
Sbjct: 84  LTPNVYLSELFGKFLFISCDLLTAFLLYRLLLLKGLGRRQACGYCVFWLLNPLPMAVSSR 143

Query: 138 GNSDMLVTFMSLMVLSKFARRKCYQAAAVLGFAVHFKIYPIIYALPLTLGVWEQS----- 192
           GN+D +V  + L  L    +R    AA   GFAVH K+YP+ Y LP+ L +  +      
Sbjct: 144 GNADSIVASLVLTTLYLIEKRLIACAAVFYGFAVHMKMYPVTYILPIALHLRPERDSDEG 203

Query: 193 --------VAASTNTWRRVVKTAVVVSICALMAAISFAVPTVLCYMKYGQQYLNEAFIYH 244
                    A   +  +R+   AV++ +   +A ++F   +   Y KYG ++L   ++YH
Sbjct: 204 LRLARYSFQARLYDFLKRLCSWAVLLFVA--IAGLTFLALSFGFYYKYGWEFLEHTYLYH 261

Query: 245 VYREDHRHNFSPYWLLMYLNMARRHLGQGVDFSPRLVAFVPQAVVLSFVSYKLRRNTAHA 304
           + R D RHNFSPY+ ++YL    +       F+  + AF+PQ ++LS  S+   R+    
Sbjct: 262 LTRRDIRHNFSPYFYMLYLTAESKW-----SFTLGIAAFLPQFILLSAASFAYYRDLVFC 316

Query: 305 CCVQTVLFVAFNKVCTVQYFVWFIPFLAFLFCEPKEVEDDESGGSGAFKFFSWVKALGVV 364
           C + T +FV FNKVCT QYF+W++  L  +                      W +A+ ++
Sbjct: 317 CFLHTSIFVTFNKVCTSQYFLWYLCLLPLVM---------------PLVRMPWKRAVVLL 361

Query: 365 LMWAATIPLWVTTAVPLEFHGYSDFAKLWIVSCLFFLAM 403
           ++W     LW+  A  LEF G + F  +W+    F L  
Sbjct: 362 MLWFIGQALWLAPAYVLEFQGKNTFLFIWLAGLFFLLIN 400
>dbj|BAB31275.1| (AK018560) data source:MGD, source key:MGI:1914806,
           evidence:ISS~phosphatidylinositol glycan, class
           M~putative [Mus musculus]
          Length = 423

 Score =  485 bits (1237), Expect = e-136
 Identities = 131/399 (32%), Positives = 202/399 (49%), Gaps = 35/399 (8%)

Query: 18  LGALLRLILIAYAFFHDQWFRVKYTDIDYMIVVDGARHMWNGGSPFDRTTFRYTPLLAAL 77
           +  L R+ L+ Y  F D+   V+YTDIDY +  D AR +  G SP+ R T+RYTPLL+ L
Sbjct: 24  VAFLARVALVFYGVFQDRTLLVRYTDIDYHVFTDAARFVTEGRSPYLRATYRYTPLLSWL 83

Query: 78  VMPSIWIANPMGKLIFASSDLGAAWYCYGVLKSFAKERSAKWMVSLFILFNPIVLSVSTR 137
           + P+++++   GK +F S DL  A+  Y +L      R       +F L NP+ ++VS+R
Sbjct: 84  LTPNVYLSELFGKFLFISCDLLTAFLLYRLLLLKGLGRRQACGYCVFWLLNPLPMAVSSR 143

Query: 138 GNSDMLVTFMSLMVLSKFARRKCYQAAAVLGFAVHFKIYPIIYALPLTLGVWEQS----- 192
           GN+D +V  + L  L    +R    AA   GFAVH K+YP+ Y LP+ L +  +      
Sbjct: 144 GNADSIVASLVLSTLYFIEKRLIACAAVFYGFAVHMKMYPVTYILPIALHLRPERDDDER 203

Query: 193 --------VAASTNTWRRVVKTAVVVSICALMAAISFAVPTVLCYMKYGQQYLNEAFIYH 244
                    A   +  RR+   AV++ +   +A ++F   +   Y KYG ++L   + YH
Sbjct: 204 LRQARFSFQARLYDFLRRLCSWAVLLFVA--VAGLTFVALSFGFYYKYGWEFLEHTYFYH 261

Query: 245 VYREDHRHNFSPYWLLMYLNMARRHLGQGVDFSPRLVAFVPQAVVLSFVSYKLRRNTAHA 304
           + R D RHNFSPY+ ++YL    +       F+  + AF+PQ ++LS  S+   R+    
Sbjct: 262 LTRRDIRHNFSPYFYMLYLTAESKW-----SFTLGIAAFLPQFILLSAASFAYYRDLVFC 316

Query: 305 CCVQTVLFVAFNKVCTVQYFVWFIPFLAFLFCEPKEVEDDESGGSGAFKFFSWVKALGVV 364
           C + T +FV FNKVCT QYF+W++  L  +                      W +A+ ++
Sbjct: 317 CFLHTSIFVTFNKVCTSQYFLWYLCLLPLVM---------------PLVRMPWKRAVVLL 361

Query: 365 LMWAATIPLWVTTAVPLEFHGYSDFAKLWIVSCLFFLAM 403
           L W     LW+  A  LEF G + F  +W+    F L  
Sbjct: 362 LFWFIGQALWLAPAYVLEFQGKNTFLFIWLAGLFFLLIN 400
>gb|AAH05650.1|AAH05650 (BC005650) RIKEN cDNA 4933437L05 gene [Mus musculus]
          Length = 423

 Score =  485 bits (1237), Expect = e-136
 Identities = 131/399 (32%), Positives = 202/399 (49%), Gaps = 35/399 (8%)

Query: 18  LGALLRLILIAYAFFHDQWFRVKYTDIDYMIVVDGARHMWNGGSPFDRTTFRYTPLLAAL 77
           +  L R+ L+ Y  F D+   V+YTDIDY +  D AR +  G SP+ R T+RYTPLL+ L
Sbjct: 24  VAFLARVALVFYGVFQDRTLLVRYTDIDYHVFTDAARFVTEGRSPYLRATYRYTPLLSWL 83

Query: 78  VMPSIWIANPMGKLIFASSDLGAAWYCYGVLKSFAKERSAKWMVSLFILFNPIVLSVSTR 137
           + P+++++   GK +F S DL  A+  Y +L      R       +F L NP+ ++VS+R
Sbjct: 84  LTPNVYLSELFGKFLFISCDLLTAFLLYRLLLLKGLGRRQACGYCVFWLLNPLPMAVSSR 143

Query: 138 GNSDMLVTFMSLMVLSKFARRKCYQAAAVLGFAVHFKIYPIIYALPLTLGVWEQS----- 192
           GN+D +V  + L  L    +R    AA   GFAVH K+YP+ Y LP+ L +  +      
Sbjct: 144 GNADSIVASLVLSTLYFIEKRLIACAAVFYGFAVHMKMYPVTYILPIALHLRPERDDDER 203

Query: 193 --------VAASTNTWRRVVKTAVVVSICALMAAISFAVPTVLCYMKYGQQYLNEAFIYH 244
                    A   +  RR+   AV++ +   +A ++F   +   Y KYG ++L   + YH
Sbjct: 204 LRHARFSFQARLYDFLRRLCSWAVLLFVA--VAGLTFVALSFGFYYKYGWEFLEHTYFYH 261

Query: 245 VYREDHRHNFSPYWLLMYLNMARRHLGQGVDFSPRLVAFVPQAVVLSFVSYKLRRNTAHA 304
           + R D RHNFSPY+ ++YL    +       F+  + AF+PQ ++LS  S+   R+    
Sbjct: 262 LTRRDIRHNFSPYFYMLYLTAESKW-----SFTLGIAAFLPQFILLSAASFAYYRDLVFC 316

Query: 305 CCVQTVLFVAFNKVCTVQYFVWFIPFLAFLFCEPKEVEDDESGGSGAFKFFSWVKALGVV 364
           C + T +FV FNKVCT QYF+W++  L  +                      W +A+ ++
Sbjct: 317 CFLHTSIFVTFNKVCTSQYFLWYLCLLPLVM---------------PLVRMPWKRAVVLL 361

Query: 365 LMWAATIPLWVTTAVPLEFHGYSDFAKLWIVSCLFFLAM 403
           L W     LW+  A  LEF G + F  +W+    F L  
Sbjct: 362 LFWFIGQALWLAPAYVLEFQGKNTFLFIWLAGLFFLLIN 400
>ref|XP_049665.1| (XM_049665) similar to PIG-M mRNA for mannosyltransferase [Homo
           sapiens]
 dbj|BAB18567.1| (AB028127) mannosyltransferase [Homo sapiens]
 gb|AAH19865.1|AAH19865 (BC019865) Unknown (protein for MGC:29896) [Homo sapiens]
          Length = 423

 Score =  479 bits (1221), Expect = e-134
 Identities = 135/399 (33%), Positives = 208/399 (51%), Gaps = 35/399 (8%)

Query: 18  LGALLRLILIAYAFFHDQWFRVKYTDIDYMIVVDGARHMWNGGSPFDRTTFRYTPLLAAL 77
           +  L R+ L+ Y  F D+   V+YTDIDY +  D AR +  G SP+ R T+RYTPLL  L
Sbjct: 24  VAFLARVALVFYGVFQDRTLHVRYTDIDYQVFTDAARFVTEGRSPYLRATYRYTPLLGWL 83

Query: 78  VMPSIWIANPMGKLIFASSDLGAAWYCYGVLKSFAKERSAKWMVSLFILFNPIVLSVSTR 137
           + P+I+++   GK +F S DL  A+  Y +L      R       +F L NP+ ++VS+R
Sbjct: 84  LTPNIYLSELFGKFLFISCDLLTAFLLYRLLLLKGLGRRQACGYCVFWLLNPLPMAVSSR 143

Query: 138 GNSDMLVTFMSLMVLSKFARRKCYQAAAVLGFAVHFKIYPIIYALPLTLGVWE-----QS 192
           GN+D +V  + LMVL    +R    AA   GFAVH KIYP+ Y LP+TL +       +S
Sbjct: 144 GNADSIVASLVLMVLYLIKKRLVACAAVFYGFAVHMKIYPVTYILPITLHLLPDRDNDKS 203

Query: 193 VAASTNTW--------RRVVKTAVVVSICALMAAISFAVPTVLCYMKYGQQYLNEAFIYH 244
           +     T+        +R+   AV++ +   +A ++F   +   Y +YG ++L   + YH
Sbjct: 204 LRQFRYTFQACLYELLKRLCNRAVLLFVA--VAGLTFFALSFGFYYEYGWEFLEHTYFYH 261

Query: 245 VYREDHRHNFSPYWLLMYLNMARRHLGQGVDFSPRLVAFVPQAVVLSFVSYKLRRNTAHA 304
           + R D RHNFSPY+ ++YL    +       FS  + AF+PQ ++LS VS+   R+    
Sbjct: 262 LTRRDIRHNFSPYFYMLYLTAESKW-----SFSLGIAAFLPQLILLSAVSFAYYRDLVFC 316

Query: 305 CCVQTVLFVAFNKVCTVQYFVWFIPFLAFLFCEPKEVEDDESGGSGAFKFFSWVKALGVV 364
           C + T +FV FNKVCT QYF+W++  L  +                      W +A+ ++
Sbjct: 317 CFLHTSIFVTFNKVCTSQYFLWYLCLLPLVM---------------PLVRMPWKRAVVLL 361

Query: 365 LMWAATIPLWVTTAVPLEFHGYSDFAKLWIVSCLFFLAM 403
           ++W     +W+  A  LEF G + F  +W+    F L  
Sbjct: 362 MLWFIGQAMWLAPAYVLEFQGKNTFLFIWLAGLFFLLIN 400
>ref|NP_496426.1| (NM_064025) B0491.1.p [Caenorhabditis elegans]
 pir||T18769 hypothetical protein B0491.1 - Caenorhabditis elegans
 emb|CAA90090.1| (Z49907) B0491.1 [Caenorhabditis elegans]
          Length = 417

 Score =  452 bits (1151), Expect = e-126
 Identities = 137/410 (33%), Positives = 210/410 (50%), Gaps = 37/410 (9%)

Query: 8   DTVSLQKLLLLGALLRLILIAYAFFHDQWFRVKYTDIDYMIVVDGARHMWNGGSPFDRTT 67
           +T +  K+LL+  + R+IL+ YA  HD  F+V +TDIDY +  D A+H+ NGGSPFDR T
Sbjct: 11  ETFNRNKILLVAFVARIILVFYAHIHDYLFKVNFTDIDYHVFSDAAKHVSNGGSPFDRAT 70

Query: 68  FRYTPLLAALVMPSIWIANPMGKLIFASSDLGAAWYCYGVLKSFAKERS----------A 117
           +RYTP LA +++P +   +  GK++F   D+  A   + +++    E             
Sbjct: 71  YRYTPALAWILLPVVHFPD-FGKILFCIFDILVAILYFKIMEKDLNETKSETREEMKDDQ 129

Query: 118 KWMVSLFILFNPIVLSVSTRGNSDMLVTFMSLMVLSKFARRKCYQAAAVLG-FAVHFKIY 176
              V ++ L NP+   +S RGN++ +V  + L+ +    +     AA V G  A+  KIY
Sbjct: 130 TMNVVIYWLANPLTAIISARGNAESIVAAVVLLNIVLLQKGYWKSAALVHGALAIQLKIY 189

Query: 177 PIIYALPLTLGV---WEQSVAASTNTWRRVVKTAVVVSICALMAAISFAVPTVLCYMKYG 233
           P+IY   + L +    EQS     N ++ +V      +   L+   SFA   +  +  YG
Sbjct: 190 PLIYLPSVFLSLSTIGEQSCV--VNKFKSLVSNWKGFAY-MLVTLTSFAAVVLFFFQIYG 246

Query: 234 QQYLNEAFIYHVYREDHRHNFSPYWLLMYLNMARRHLGQGVDFSPRLVAFVPQAVVLSFV 293
           Q +L+E  IYHV R D  HNFSPY+ L+YL  A   + Q +     L AF+PQ V++ F 
Sbjct: 247 QLFLDEYLIYHVKRRDLAHNFSPYFYLLYLYEANPTMSQII----GLGAFIPQIVLIVFF 302

Query: 294 SYKLRRNTAHACCVQTVLFVAFNKVCTVQYFVWFIPFLAFLFCEPKEVEDDESGGSGAFK 353
           ++K   +      + T  FV +NKVCT QYFVW+I  L  L  +                
Sbjct: 303 AFKHYDDLPFCWFITTFAFVTYNKVCTSQYFVWYIVLLPLLAHK---------------I 347

Query: 354 FFSWVKALGVVLMWAATIPLWVTTAVPLEFHGYSDFAKLWIVSCLFFLAM 403
             S   AL ++  W AT  +W+  A   EF G++ F  +++ SCLF +A 
Sbjct: 348 MMSRQLALSLMAAWFATQGIWLLAAYLFEFQGWNTFFLMFLASCLFLIAN 397
>emb|CAB89880.1| (AL354632) hypothetical transmembrane protein [Schizosaccharomyces
           pombe]
          Length = 815

 Score =  442 bits (1126), Expect = e-123
 Identities = 129/392 (32%), Positives = 202/392 (50%), Gaps = 26/392 (6%)

Query: 15  LLLLGALLRLILIAYAFFHDQWFRVKYTDIDYMIVVDGARHMWNGGSPFDRTTFRYTPLL 74
           LLLLG L+RL+L+ Y  +HD+   +K+TDIDY +  D ++++  G SP+ R T+RYTP+L
Sbjct: 438 LLLLGLLMRLVLLNYGIWHDRRSALKFTDIDYFVFSDASKYVSIGMSPYMRDTYRYTPML 497

Query: 75  AALVMPSIWIANPMGKLIFASSDLGAAWYCYGVL-KSFAKERSAKWMVSLFILFNPIVLS 133
           A L++P+ +     GK +F+ SDL A W    +L +  + +RS   + S F + NP V  
Sbjct: 498 AILLLPTQYGFPSWGKYLFSISDLIAGWLMIKLLSRRISYKRSL--IYSSFWILNPFVAI 555

Query: 134 VSTRGNSDMLVTFMSLMVLSKFARRKCYQAAAVLGFAVHFKIYPIIYALPLTLGVWEQSV 193
           +STRGN + ++  +S+ +L    ++  + A+ +LGF+VHFKIYP +Y +   L  + +  
Sbjct: 556 ISTRGNCEAILGILSIALLYLIEKKSVWLASLILGFSVHFKIYPFMYGIAF-LVYFSKPK 614

Query: 194 AASTNTWRRVVKTAVVVSICALMAAISFAVPTVLCYMKYGQQYLNEAFIYHVYREDHRHN 253
             ST   + +   ++      + +   F +  +L Y  YG  +L   ++YH  R DHRHN
Sbjct: 615 KGSTFMEKFLSLLSINQLKIVVGSLFMFTICNLLMYYLYGSPFLEHTYLYHFGRTDHRHN 674

Query: 254 FSPYWLLMYLNMARRHLGQGVDFSPRLVAFVPQAVVLSFVSYKL-RRNTAHACCVQTVLF 312
           FS + L +Y   +    G        L AF+PQ  +   +     ++N       QT  F
Sbjct: 675 FSLHHLNLYYESS---FGAKAS---SLFAFLPQLSLCMLIPLVFGKKNLPGTLFAQTFAF 728

Query: 313 VAFNKVCTVQYFVWFIPFLAFLFCEPKEVEDDESGGSGAFKFFSWVKALGVVLMWAATIP 372
           V FNKVCT QYF+W++ FL  +    K +                 K L  + +W     
Sbjct: 729 VTFNKVCTSQYFMWYLVFLPLVLPNSKLLS---------------KKGLICLSLWIIGQL 773

Query: 373 LWVTTAVPLEFHGYSDFAKLWIVSCLFFLAMV 404
           LW+ +A  LE  G S F  LW+   LFF   V
Sbjct: 774 LWLISAYNLEMLGKSVFIPLWLSGLLFFFFNV 805
>emb|CAC34506.1| (AL589883) mannosyltransferase-like protein [Arabidopsis thaliana]
          Length = 417

 Score =  371 bits (945), Expect = e-102
 Identities = 122/428 (28%), Positives = 185/428 (42%), Gaps = 87/428 (20%)

Query: 10  VSLQKLLLLGALLRLILIAYAFFHDQWFRVKYTDIDYMIVVDGARHMWNGGSPFDRTTFR 69
           +  + LL+   LLR+ LI Y  + D    V+YTD+DY++  D A  M +G SP+ RTT+R
Sbjct: 16  MKFRSLLVFSMLLRVFLIVYGEWQDAHMEVRYTDVDYIVFSDAASLMASGESPYKRTTYR 75

Query: 70  YTPLLAALVMPSIWIANPMGKLIFASSDLGAAWYCYGVLKSFAKERSAKWMVSLFILFNP 129
           Y+PLLA L+ P+ +     GK +F+                                   
Sbjct: 76  YSPLLALLLTPNSFFHRSWGKFLFS----------------------------------- 100

Query: 130 IVLSVSTRGNSDMLVTFMSLMVLSKFA----RRKCYQAAAVLGFAVHFKIYPIIYALPLT 185
              SV    N  M+   + L  +S F          QAA   G  VHF++YPIIYALP+ 
Sbjct: 101 --ASVHQPHNVSMICFSVVLESISMFQIYWSAGNLLQAAFWYGLVVHFRVYPIIYALPII 158

Query: 186 LGVWE-----------------QSVAASTNTWRRVVKTAVVVSI----------CALMAA 218
           L +                   Q+   ++N  R+     ++ ++           AL++ 
Sbjct: 159 LVLDTQVFRSGQKPALLYWNTGQAKTPASNMERKTFLFNLLTTLKSLFSRERIMFALISG 218

Query: 219 ISFAVPTVLCYMKYGQQYLNEAFIYHVYREDHRHNFSPYWLLMYLNMARRHLGQGVDFSP 278
             F     + +  YGQ++L+EA +YH+ R D RHNFS Y+  +YL+  R+          
Sbjct: 219 GVFLACNAVSFYFYGQEFLHEALLYHLTRTDPRHNFSIYFYHIYLHYERQ-----FSAVE 273

Query: 279 RLVAFVPQAVVLSFVSYKLRRNTAHACCVQTVLFVAFNKVCTVQYFVWFIPFLAFLFCEP 338
           +L++F+PQ  V   + +   ++      +QTV FV FNKV T QYFVWF   L  +    
Sbjct: 274 KLISFLPQFTVQFALVFCFSQDLVFCIFLQTVAFVTFNKVITAQYFVWFYCLLPLILP-- 331

Query: 339 KEVEDDESGGSGAFKFFSWVKALGVVLMWAATIPLWVTTAVPLEFHGYSDFAKLWIVSCL 398
                       +     W + L  ++MW      W+     LEF G + F  LWI S L
Sbjct: 332 -----------WSHMKLKW-EGLLCIIMWIGAQTHWLLWGYMLEFKGVNVFLPLWIASLL 379

Query: 399 FFLAMVVL 406
           F  A   +
Sbjct: 380 FLAANTFV 387
>ref|NP_012547.1| (NC_001142) Protein required for cell viability; Yjr013wp
           [Saccharomyces cerevisiae]
 sp|P47088|YJY3_YEAST HYPOTHETICAL 35.6 KD PROTEIN IN SPC1-ILV3 INTERGENIC REGION
 pir||S55201 hypothetical protein YJR013w - yeast (Saccharomyces cerevisiae)
 emb|CAA60935.1| (X87611) ORF YJR83.11 [Saccharomyces cerevisiae]
 emb|CAA89537.1| (Z49513) ORF YJR013w [Saccharomyces cerevisiae]
          Length = 305

 Score =  304 bits (773), Expect = 1e-81
 Identities = 94/306 (30%), Positives = 150/306 (48%), Gaps = 32/306 (10%)

Query: 108 LKSFAKERSAKWMVSLFILFNPIVLSVSTRGNSDMLVTFMSLMVLSKFARRKCYQAAAVL 167
           L + A  R    ++    L NP+V+++STRGN++ ++  + +  L    + +   A  + 
Sbjct: 3   LLNQAISRKRALILESIWLLNPMVITISTRGNAESVLCCLIMFTLFFLQKSRYTLAGILY 62

Query: 168 GFAVHFKIYPIIYALPLTLGVWEQSVAASTNTWRRVVKTAVVVSICALMAAISFAVPTVL 227
           G ++HFKIYPIIY +P+ + ++         T    +    + ++  L+           
Sbjct: 63  GLSIHFKIYPIIYCIPIAIFIYYNKRNQGPRTQLTSLLNIGLSTLTTLLGCG------WA 116

Query: 228 CYMKYGQQYLNEAFIYHVYREDHRHNFSPYWLLMYLNMARRHLGQGVDFSPRLVAFVPQA 287
            Y  YG ++L++A++YH+YR DHRHNFS + +L+YL+ A +  G+    +    AFVPQ 
Sbjct: 117 MYKIYGYEFLDQAYLYHLYRTDHRHNFSVWNMLLYLDSANKENGES---NLSRYAFVPQ- 172

Query: 288 VVLSFVSYKLR------RNTAHACCVQTVLFVAFNKVCTVQYFVWFIPFLAFLFCEPKEV 341
           ++L  V+  L        N      VQT  FV +NKVCT QYFVW++ FL F        
Sbjct: 173 LLLVLVTGCLEWWNPTFDNLLRVLFVQTFAFVTYNKVCTSQYFVWYLIFLPFYLSRTH-- 230

Query: 342 EDDESGGSGAFKFFSWVKALGVVLMWAATIPLWVTTAVPLEFHGYSDFAK-LWIVSCLFF 400
                          W K L +  +W  T  +W++    LEF G + F   L+I S LFF
Sbjct: 231 -------------IGWKKGLLMATLWVGTQGIWLSQGYYLEFEGKNVFYPGLFIASVLFF 277

Query: 401 LAMVVL 406
           +  V L
Sbjct: 278 VTNVWL 283
>ref|XP_080607.1| (XM_080607) CG9865 [Drosophila melanogaster]
 gb|AAF46748.1| (AE003454) CG9865 gene product [Drosophila melanogaster]
 gb|AAK77251.1| (AY047519) GH02741p [Drosophila melanogaster]
          Length = 466

 Score =  299 bits (759), Expect = 6e-80
 Identities = 72/314 (22%), Positives = 130/314 (40%), Gaps = 32/314 (10%)

Query: 109 KSFAKERSAKWMVSLFILFNPIVLSVSTRGNSDMLVTFMSLMVLSKFARRK--------- 159
                 R++      F L+NP+   +STRG+ D   +F  ++ +    + +         
Sbjct: 156 HPENLARASAC----FWLYNPLTAVISTRGSGDCFSSFFVILTIYLLLKSEHNVTRSYWL 211

Query: 160 CYQAAAVLGFAVHFKIYPIIYALPLTLGVWEQSVAASTNTWRRVVKTAVVVSICALMAAI 219
            + A    G  +H ++YP++++L   L +  +      +   ++++             I
Sbjct: 212 IFGAGLAHGLVIHLRLYPLLFSLAYYLSLSTRLTQTPLDFLCQILRPNKQQLCLISGTLI 271

Query: 220 SFAVPTVLCYMKYGQQYLNEAFIYHVYREDHRHNFSPYWLLMYLNMARRHLGQGVDFSPR 279
           S    T   Y  YG +Y+ EA++YH  R+D RHNFS  +LL YL  A             
Sbjct: 272 SLVAFTWTFYTMYGWEYIYEAYLYHFVRKDPRHNFSLQFLLQYLGSASSVAEPSAILKTL 331

Query: 280 LVAFVPQAVVLSFVSY---KLRRNTAHACCVQTVLFVAFNKVCTVQYFVWFIPFLAFLFC 336
           ++A  PQ +++ ++S    + R+           + V +N V T QYF+W++  L     
Sbjct: 332 VLA--PQFLLILYLSLSFGQFRQTLPFCIFAVAFVIVTYNSVVTSQYFIWYLAILPLCL- 388

Query: 337 EPKEVEDDESGGSGAFKFFSWVKALGVVLMWAATIPLWVTTAVPLEFHGYSDFAKLWIVS 396
                          FK  SW +  G++ +W     LW+  A  LEFH ++ F  + +  
Sbjct: 389 -------------NNFKMLSWRRCFGLLFLWLLAQALWLLPAYLLEFHTWNTFYWIGLQG 435

Query: 397 CLFFLAMVVLASML 410
            +FF     +   L
Sbjct: 436 AVFFATNGYILEQL 449
 Score =  155 bits (389), Expect = 1e-36
 Identities = 48/118 (40%), Positives = 67/118 (56%)

Query: 11  SLQKLLLLGALLRLILIAYAFFHDQWFRVKYTDIDYMIVVDGARHMWNGGSPFDRTTFRY 70
           S +  LL+ ALLR+ LI Y   HD    V YTDIDY +V DGAR +  G +PF R T+RY
Sbjct: 22  SFRTHLLISALLRIALICYGQLHDSQSAVPYTDIDYKVVTDGARQVLAGDTPFARHTYRY 81

Query: 71  TPLLAALVMPSIWIANPMGKLIFASSDLGAAWYCYGVLKSFAKERSAKWMVSLFILFN 128
           +P++A L   +I +    GKL++A+ DL  A   Y ++    K +  K +  L   FN
Sbjct: 82  SPIMAYLQTFNILLHPAWGKLLYATFDLLIATLIYRLVHMEIKSQYQKTVQHLLSKFN 139
>dbj|BAA87228.1| (AB027924) Hypothetical protein [Schizosaccharomyces pombe]
          Length = 149

 Score =  166 bits (418), Expect = 6e-40
 Identities = 40/147 (27%), Positives = 74/147 (50%), Gaps = 4/147 (2%)

Query: 104 CYGVL-KSFAKERSAKWMVSLFILFNPIVLSVSTRGNSDMLVTFMSLMVLSKFARRKCYQ 162
              +L +  + +RS   + S F + NP V  +STRGN + ++  +S+ +L    ++  + 
Sbjct: 6   MIKLLSRRISYKRSL--IYSSFWILNPFVAIISTRGNCEAILGILSIALLYLIEKKSVWL 63

Query: 163 AAAVLGFAVHFKIYPIIYALPLTLGVWEQSVAASTNTWRRVVKTAVVVSICALMAAISFA 222
           A+ +LGF+VHFKIYP +Y +   L  + +    ST   + +   ++      + +   F 
Sbjct: 64  ASLILGFSVHFKIYPFMYGIAF-LVYFSKPKKGSTFMEKFLSLLSINQLKIVVGSLFMFT 122

Query: 223 VPTVLCYMKYGQQYLNEAFIYHVYRED 249
           +  +L Y  YG  +L   ++YH  R D
Sbjct: 123 ICNLLMYYLYGSPFLEHTYLYHFGRTD 149
>ref|NP_346994.1| (NC_003030) Uncharacterized membrane protein [Clostridium
           acetobutylicum]
 gb|AAK78334.1|AE007550_1 (AE007550) Uncharacterized membrane protein [Clostridium
           acetobutylicum]
          Length = 544

 Score = 43.9 bits (103), Expect = 0.005
 Identities = 43/221 (19%), Positives = 91/221 (40%), Gaps = 31/221 (14%)

Query: 16  LLLGALLRLI--LIAYAFFHDQWFRVKYTDIDYMIVVDGARHMWNGGSPFDRTTFRYTPL 73
           L++G L R+I  L+   +  D     ++        +  A+++      +   +  Y P+
Sbjct: 151 LVIGFLFRIIVGLLITGYVTDIGLYKQWA-------LTAAKNLAA---VYTNGSVDYPPV 200

Query: 74  LAALV---------MPSIWIANPMGKLIFASSDLGAAWYCYGV-LKSFAKERSAKWMVSL 123
              ++               +  + KL    +D+ +A++ Y + +K F+ E S     +L
Sbjct: 201 YLYVLSIIGKIASSSFMSNYSTLLIKLPSIIADIFSAYFVYRLSIKRFSYETSFAL--TL 258

Query: 124 FILFNPIVLSVSTR-GNSDMLVTFMSLMVLSKFARRKCYQAAAVLGFAVHFKIYPIIYAL 182
           F LFNP +L  ST  G +D   T + ++ +   +  K   ++     +V  K   II+ L
Sbjct: 259 FYLFNPAILINSTLWGQADSFFTLLVILAVYFISEDKIVPSSIFFALSVLMKPQGIIF-L 317

Query: 183 PLTLGVWEQSVAASTNTWRRVVKTAVVVSICALMAAISFAV 223
           PL L  + ++        + + K+     I  ++    F++
Sbjct: 318 PLVLFWYIKNKN-----LKSIFKSIASGFITTIIVLAPFSI 353
CPU time:   124.45 user secs.	    2.42 sys. secs	  126.87 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.322    0.172    0.555 

Gapped
Lambda     K      H
   0.270   0.0604    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 311110194
Number of Sequences: 887402
Number of extensions: 16285222
Number of successful extensions: 64185
Number of sequences better than 10.0: 305
Number of HSP's better than 10.0 without gapping: 20
Number of HSP's successfully gapped in prelim test: 287
Number of HSP's that attempted gapping in prelim test: 63857
Number of HSP's gapped (non-prelim): 490
length of query: 430
length of database: 277,845,442
effective HSP length: 51
effective length of query: 379
effective length of database: 232,587,940
effective search space: 88150829260
effective search space used: 88150829260
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (21.1 bits)
S2: 75 (33.3 bits)