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GPI Anchor Biosynthesis Report: AAF46748.1 (PIG-M family, Drosophila melanogaster)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query=
         (466 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!


Results of PSI-Blast iteration 1

Distribution of 19 Blast Hits on the Query Sequence


Sequences with E-value BETTER than threshold (0.002)

ref|XP_080607.1| (XM_080607) CG9865 [Drosophila melanogaster] >g... 629 e-179
ref|NP_496426.1| (NM_064025) B0491.1.p [Caenorhabditis elegans] ... 492 e-138
ref|XP_049665.1| (XM_049665) similar to PIG-M mRNA for mannosylt... 472 e-132
dbj|BAB31275.1| (AK018560) data source:MGD, source key:MGI:19148... 364 1e-99
gb|AAH05650.1|AAH05650 (BC005650) RIKEN cDNA 4933437L05 gene [Mu... 362 7e-99
ref|NP_077058.1| (NM_024144) PIG-M mRNA for mannosyltransferase ... 360 4e-98
emb|CAB89880.1| (AL354632) hypothetical transmembrane protein [S... 330 3e-89
dbj|BAB20994.1| (AB050105) TbPIG-M [Trypanosoma brucei] 329 9e-89
ref|NP_012547.1| (NC_001142) Protein required for cell viability... 321 2e-86
emb|CAC34506.1| (AL589883) mannosyltransferase-like protein [Ara... 300 3e-80
dbj|BAA87228.1| (AB027924) Hypothetical protein [Schizosaccharom... 145 1e-33
Sequences with E-value WORSE than threshold

pir||T36589 probable transmembrane protein - Streptomyces coelic... 39 0.20
Alignments
>ref|XP_080607.1| (XM_080607) CG9865 [Drosophila melanogaster]
 gb|AAF46748.1| (AE003454) CG9865 gene product [Drosophila melanogaster]
 gb|AAK77251.1| (AY047519) GH02741p [Drosophila melanogaster]
          Length = 466

 Score =  629 bits (1607), Expect = e-179
 Identities = 466/466 (100%), Positives = 466/466 (100%)

Query: 1   MTQTKAATTGWQRLGRRILYTSFRTHLLISALLRIALICYGQLHDSQSAVPYTDIDYKVV 60
           MTQTKAATTGWQRLGRRILYTSFRTHLLISALLRIALICYGQLHDSQSAVPYTDIDYKVV
Sbjct: 1   MTQTKAATTGWQRLGRRILYTSFRTHLLISALLRIALICYGQLHDSQSAVPYTDIDYKVV 60

Query: 61  TDGARQVLAGDTPFARHTYRYSPIMAYLQTFNILLHPAWGKLLYATFDLLIATLIYRLVH 120
           TDGARQVLAGDTPFARHTYRYSPIMAYLQTFNILLHPAWGKLLYATFDLLIATLIYRLVH
Sbjct: 61  TDGARQVLAGDTPFARHTYRYSPIMAYLQTFNILLHPAWGKLLYATFDLLIATLIYRLVH 120

Query: 121 MEIKSQYQKTVQHLLSKFNRPRDSDQSLDALDERSHPENLARASACFWLYNPLTAVISTR 180
           MEIKSQYQKTVQHLLSKFNRPRDSDQSLDALDERSHPENLARASACFWLYNPLTAVISTR
Sbjct: 121 MEIKSQYQKTVQHLLSKFNRPRDSDQSLDALDERSHPENLARASACFWLYNPLTAVISTR 180

Query: 181 GSGDCFSSFFVILTIYLLLKSEHNVTRSYWLIFGAGLAHGLVIHLRLYPLLFSLAYYLSL 240
           GSGDCFSSFFVILTIYLLLKSEHNVTRSYWLIFGAGLAHGLVIHLRLYPLLFSLAYYLSL
Sbjct: 181 GSGDCFSSFFVILTIYLLLKSEHNVTRSYWLIFGAGLAHGLVIHLRLYPLLFSLAYYLSL 240

Query: 241 STRLTQTPLDFLCQILRPNKQQLCLISGTLISLVAFTWTFYTMYGWEYIYEAYLYHFVRK 300
           STRLTQTPLDFLCQILRPNKQQLCLISGTLISLVAFTWTFYTMYGWEYIYEAYLYHFVRK
Sbjct: 241 STRLTQTPLDFLCQILRPNKQQLCLISGTLISLVAFTWTFYTMYGWEYIYEAYLYHFVRK 300

Query: 301 DPRHNFSLQFLLQYLGSASSVAEPSAILKTLVLAPQFLLILYLSLSFGQFRQTLPFCIFA 360
           DPRHNFSLQFLLQYLGSASSVAEPSAILKTLVLAPQFLLILYLSLSFGQFRQTLPFCIFA
Sbjct: 301 DPRHNFSLQFLLQYLGSASSVAEPSAILKTLVLAPQFLLILYLSLSFGQFRQTLPFCIFA 360

Query: 361 VAFVIVTYNSVVTSQYFIWYLAILPLCLNNFKMLSWRRCFGLLFLWLLAQALWLLPAYLL 420
           VAFVIVTYNSVVTSQYFIWYLAILPLCLNNFKMLSWRRCFGLLFLWLLAQALWLLPAYLL
Sbjct: 361 VAFVIVTYNSVVTSQYFIWYLAILPLCLNNFKMLSWRRCFGLLFLWLLAQALWLLPAYLL 420

Query: 421 EFHTWNTFYWIGLQGAVFFATNGYILEQLLSHYGFTQFKISYRKLL 466
           EFHTWNTFYWIGLQGAVFFATNGYILEQLLSHYGFTQFKISYRKLL
Sbjct: 421 EFHTWNTFYWIGLQGAVFFATNGYILEQLLSHYGFTQFKISYRKLL 466
>ref|NP_496426.1| (NM_064025) B0491.1.p [Caenorhabditis elegans]
 pir||T18769 hypothetical protein B0491.1 - Caenorhabditis elegans
 emb|CAA90090.1| (Z49907) B0491.1 [Caenorhabditis elegans]
          Length = 417

 Score =  492 bits (1255), Expect = e-138
 Identities = 150/430 (34%), Positives = 229/430 (52%), Gaps = 39/430 (9%)

Query: 25  THLLISALLRIALICYGQLHDSQSAVPYTDIDYKVVTDGARQVLAGDTPFARHTYRYSPI 84
             LL++ + RI L+ Y  +HD    V +TDIDY V +D A+ V  G +PF R TYRY+P 
Sbjct: 17  KILLVAFVARIILVFYAHIHDYLFKVNFTDIDYHVFSDAAKHVSNGGSPFDRATYRYTPA 76

Query: 85  MAYLQTFNILLHPAWGKLLYATFDLLIATLIYRLVHMEIKSQYQKTVQHLLSKFNRPRDS 144
           +A++    ++  P +GK+L+  FD+L+A L ++++  ++     +T + +          
Sbjct: 77  LAWILLP-VVHFPDFGKILFCIFDILVAILYFKIMEKDLNETKSETREEM--------KD 127

Query: 145 DQSLDALDERSHPENLARASACFWLYNPLTAVISTRGSGDCFSSFFVILTIYLLLKSEHN 204
           DQ+++ +               +WL NPLTA+IS RG+ +   +  V+L I LL K    
Sbjct: 128 DQTMNVV--------------IYWLANPLTAIISARGNAESIVAAVVLLNIVLLQK---- 169

Query: 205 VTRSYWLIFGAGLAHG-LVIHLRLYPLLFSLAYYLSLSTRLTQTPLDFLCQILRPNKQQL 263
               YW    A L HG L I L++YPL++  + +LSLST   Q+ +    + L  N +  
Sbjct: 170 ---GYWK--SAALVHGALAIQLKIYPLIYLPSVFLSLSTIGEQSCVVNKFKSLVSNWKGF 224

Query: 264 CLISGTLISLVAFTWTFYTMYGWEYIYEAYLYHFVRKDPRHNFSLQFLLQYLGSASSVAE 323
             +  TL S  A    F+ +YG  ++ E  +YH  R+D  HNFS  F L YL  A+    
Sbjct: 225 AYMLVTLTSFAAVVLFFFQIYGQLFLDEYLIYHVKRRDLAHNFSPYFYLLYLYEANPTM- 283

Query: 324 PSAILKTLVLAPQFLLILYLSLSFGQFRQTLPFCIFAVAFVIVTYNSVVTSQYFIWYLAI 383
            S I+      PQ +LI++ +    +    LPFC F   F  VTYN V TSQYF+WY+ +
Sbjct: 284 -SQIIGLGAFIPQIVLIVFFAF---KHYDDLPFCWFITTFAFVTYNKVCTSQYFVWYIVL 339

Query: 384 LPLCLNNFKMLSWRRCFGLLFLWLLAQALWLLPAYLLEFHTWNTFYWIGLQGAVFFATNG 443
           LPL  +   M+S +    L+  W   Q +WLL AYL EF  WNTF+ + L   +F   N 
Sbjct: 340 LPLLAHKI-MMSRQLALSLMAAWFATQGIWLLAAYLFEFQGWNTFFLMFLASCLFLIANS 398

Query: 444 YILEQLLSHY 453
           +IL+Q+++HY
Sbjct: 399 FILKQIINHY 408
>ref|XP_049665.1| (XM_049665) similar to PIG-M mRNA for mannosyltransferase [Homo
           sapiens]
 dbj|BAB18567.1| (AB028127) mannosyltransferase [Homo sapiens]
 gb|AAH19865.1|AAH19865 (BC019865) Unknown (protein for MGC:29896) [Homo sapiens]
          Length = 423

 Score =  472 bits (1204), Expect = e-132
 Identities = 157/436 (36%), Positives = 225/436 (51%), Gaps = 59/436 (13%)

Query: 29  ISALLRIALICYGQLHDSQSAVPYTDIDYKVVTDGARQVLAGDTPFARHTYRYSPIMAYL 88
           ++ L R+AL+ YG   D    V YTDIDY+V TD AR V  G +P+ R TYRY+P++ +L
Sbjct: 24  VAFLARVALVFYGVFQDRTLHVRYTDIDYQVFTDAARFVTEGRSPYLRATYRYTPLLGWL 83

Query: 89  QTFNILLHPAWGKLLYATFDLLIATLIYRLVHMEIKSQYQKTVQHLLSKFNRPRDSDQSL 148
            T NI L   +GK L+ + DLL A L+YRL+ ++   + Q                    
Sbjct: 84  LTPNIYLSELFGKFLFISCDLLTAFLLYRLLLLKGLGRRQ-------------------- 123

Query: 149 DALDERSHPENLARASACFWLYNPLTAVISTRGSGDCFSSFFVILTIYLLLKSEHNVTRS 208
                       A     FWL NPL   +S+RG+ D   +  V++ +YL+ K        
Sbjct: 124 ------------ACGYCVFWLLNPLPMAVSSRGNADSIVASLVLMVLYLIKKR------- 164

Query: 209 YWLIFGAGLAHGLVIHLRLYPLLFSLAYYLSL------STRLTQTPLDF---LCQILRP- 258
             L+  A + +G  +H+++YP+ + L   L L         L Q    F   L ++L+  
Sbjct: 165 --LVACAAVFYGFAVHMKIYPVTYILPITLHLLPDRDNDKSLRQFRYTFQACLYELLKRL 222

Query: 259 -NKQQLCLISGTLISLVAFTWTFYTMYGWEYIYEAYLYHFVRKDPRHNFSLQFLLQYLGS 317
            N+  L  ++   ++  A ++ FY  YGWE++   Y YH  R+D RHNFS  F + YL +
Sbjct: 223 CNRAVLLFVAVAGLTFFALSFGFYYEYGWEFLEHTYFYHLTRRDIRHNFSPYFYMLYLTA 282

Query: 318 ASSVAEPSAILKTLVLAPQFLLILYLSLSFGQFRQTLPFCIFAVAFVIVTYNSVVTSQYF 377
            S   + S  L      PQ  LIL  ++SF  +R  L FC F    + VT+N V TSQYF
Sbjct: 283 ES---KWSFSLGIAAFLPQ--LILLSAVSFAYYRD-LVFCCFLHTSIFVTFNKVCTSQYF 336

Query: 378 IWYLAILPLCLNNFKMLSWRRCFGLLFLWLLAQALWLLPAYLLEFHTWNTFYWIGLQGAV 437
           +WYL +LPL +   +M  W+R   LL LW + QA+WL PAY+LEF   NTF +I L G  
Sbjct: 337 LWYLCLLPLVMPLVRM-PWKRAVVLLMLWFIGQAMWLAPAYVLEFQGKNTFLFIWLAGLF 395

Query: 438 FFATNGYILEQLLSHY 453
           F   N  IL Q++SHY
Sbjct: 396 FLLINCSILIQIISHY 411
>dbj|BAB31275.1| (AK018560) data source:MGD, source key:MGI:1914806,
           evidence:ISS~phosphatidylinositol glycan, class
           M~putative [Mus musculus]
          Length = 423

 Score =  364 bits (927), Expect = 1e-99
 Identities = 118/336 (35%), Positives = 166/336 (49%), Gaps = 31/336 (9%)

Query: 133 HLLSKFNRPRDSDQSLDALDERSHPENLARASAC----FWLYNPLTAVISTRGSGDCFSS 188
            L  KF        +   L      + L R  AC    FWL NPL   +S+RG+ D   +
Sbjct: 92  ELFGKFLFISCDLLTAFLLYRLLLLKGLGRRQACGYCVFWLLNPLPMAVSSRGNADSIVA 151

Query: 189 FFVILTIYLLLKSEHNVTRSYWLIFGAGLAHGLVIHLRLYPLLFSLAYYLSL------ST 242
             V+ T+Y + K          LI  A + +G  +H+++YP+ + L   L L        
Sbjct: 152 SLVLSTLYFIEKR---------LIACAAVFYGFAVHMKMYPVTYILPIALHLRPERDDDE 202

Query: 243 RLTQTPLDFLCQILRP-----NKQQLCLISGTLISLVAFTWTFYTMYGWEYIYEAYLYHF 297
           RL Q    F  ++        +   L  ++   ++ VA ++ FY  YGWE++   Y YH 
Sbjct: 203 RLRQARFSFQARLYDFLRRLCSWAVLLFVAVAGLTFVALSFGFYYKYGWEFLEHTYFYHL 262

Query: 298 VRKDPRHNFSLQFLLQYLGSASSVAEPSAILKTLVLAPQFLLILYLSLSFGQFRQTLPFC 357
            R+D RHNFS  F + YL + S   + S  L      PQF+L+   + SF  +R  L FC
Sbjct: 263 TRRDIRHNFSPYFYMLYLTAES---KWSFTLGIAAFLPQFILL--SAASFAYYRD-LVFC 316

Query: 358 IFAVAFVIVTYNSVVTSQYFIWYLAILPLCLNNFKMLSWRRCFGLLFLWLLAQALWLLPA 417
            F    + VT+N V TSQYF+WYL +LPL +   +M  W+R   LL  W + QALWL PA
Sbjct: 317 CFLHTSIFVTFNKVCTSQYFLWYLCLLPLVMPLVRM-PWKRAVVLLLFWFIGQALWLAPA 375

Query: 418 YLLEFHTWNTFYWIGLQGAVFFATNGYILEQLLSHY 453
           Y+LEF   NTF +I L G  F   N  IL Q++SHY
Sbjct: 376 YVLEFQGKNTFLFIWLAGLFFLLINCSILIQIISHY 411
 Score =  129 bits (323), Expect = 9e-29
 Identities = 39/84 (46%), Positives = 54/84 (63%)

Query: 29  ISALLRIALICYGQLHDSQSAVPYTDIDYKVVTDGARQVLAGDTPFARHTYRYSPIMAYL 88
           ++ L R+AL+ YG   D    V YTDIDY V TD AR V  G +P+ R TYRY+P++++L
Sbjct: 24  VAFLARVALVFYGVFQDRTLLVRYTDIDYHVFTDAARFVTEGRSPYLRATYRYTPLLSWL 83

Query: 89  QTFNILLHPAWGKLLYATFDLLIA 112
            T N+ L   +GK L+ + DLL A
Sbjct: 84  LTPNVYLSELFGKFLFISCDLLTA 107
>gb|AAH05650.1|AAH05650 (BC005650) RIKEN cDNA 4933437L05 gene [Mus musculus]
          Length = 423

 Score =  362 bits (921), Expect = 7e-99
 Identities = 117/336 (34%), Positives = 165/336 (48%), Gaps = 31/336 (9%)

Query: 133 HLLSKFNRPRDSDQSLDALDERSHPENLARASAC----FWLYNPLTAVISTRGSGDCFSS 188
            L  KF        +   L      + L R  AC    FWL NPL   +S+RG+ D   +
Sbjct: 92  ELFGKFLFISCDLLTAFLLYRLLLLKGLGRRQACGYCVFWLLNPLPMAVSSRGNADSIVA 151

Query: 189 FFVILTIYLLLKSEHNVTRSYWLIFGAGLAHGLVIHLRLYPLLFSLAYYLSL------ST 242
             V+ T+Y + K          LI  A + +G  +H+++YP+ + L   L L        
Sbjct: 152 SLVLSTLYFIEKR---------LIACAAVFYGFAVHMKMYPVTYILPIALHLRPERDDDE 202

Query: 243 RLTQTPLDFLCQILRP-----NKQQLCLISGTLISLVAFTWTFYTMYGWEYIYEAYLYHF 297
           RL      F  ++        +   L  ++   ++ VA ++ FY  YGWE++   Y YH 
Sbjct: 203 RLRHARFSFQARLYDFLRRLCSWAVLLFVAVAGLTFVALSFGFYYKYGWEFLEHTYFYHL 262

Query: 298 VRKDPRHNFSLQFLLQYLGSASSVAEPSAILKTLVLAPQFLLILYLSLSFGQFRQTLPFC 357
            R+D RHNFS  F + YL + S   + S  L      PQF+L+   + SF  +R  L FC
Sbjct: 263 TRRDIRHNFSPYFYMLYLTAES---KWSFTLGIAAFLPQFILL--SAASFAYYRD-LVFC 316

Query: 358 IFAVAFVIVTYNSVVTSQYFIWYLAILPLCLNNFKMLSWRRCFGLLFLWLLAQALWLLPA 417
            F    + VT+N V TSQYF+WYL +LPL +   +M  W+R   LL  W + QALWL PA
Sbjct: 317 CFLHTSIFVTFNKVCTSQYFLWYLCLLPLVMPLVRM-PWKRAVVLLLFWFIGQALWLAPA 375

Query: 418 YLLEFHTWNTFYWIGLQGAVFFATNGYILEQLLSHY 453
           Y+LEF   NTF +I L G  F   N  IL Q++SHY
Sbjct: 376 YVLEFQGKNTFLFIWLAGLFFLLINCSILIQIISHY 411
 Score =  129 bits (323), Expect = 9e-29
 Identities = 39/84 (46%), Positives = 54/84 (63%)

Query: 29  ISALLRIALICYGQLHDSQSAVPYTDIDYKVVTDGARQVLAGDTPFARHTYRYSPIMAYL 88
           ++ L R+AL+ YG   D    V YTDIDY V TD AR V  G +P+ R TYRY+P++++L
Sbjct: 24  VAFLARVALVFYGVFQDRTLLVRYTDIDYHVFTDAARFVTEGRSPYLRATYRYTPLLSWL 83

Query: 89  QTFNILLHPAWGKLLYATFDLLIA 112
            T N+ L   +GK L+ + DLL A
Sbjct: 84  LTPNVYLSELFGKFLFISCDLLTA 107
>ref|NP_077058.1| (NM_024144) PIG-M mRNA for mannosyltransferase [Rattus norvegicus]
 dbj|BAB18566.1| (AB028126) mannosyltransferase [Rattus norvegicus]
          Length = 423

 Score =  360 bits (915), Expect = 4e-98
 Identities = 118/336 (35%), Positives = 167/336 (49%), Gaps = 31/336 (9%)

Query: 133 HLLSKFNRPRDSDQSLDALDERSHPENLARASAC----FWLYNPLTAVISTRGSGDCFSS 188
            L  KF        +   L      + L R  AC    FWL NPL   +S+RG+ D   +
Sbjct: 92  ELFGKFLFISCDLLTAFLLYRLLLLKGLGRRQACGYCVFWLLNPLPMAVSSRGNADSIVA 151

Query: 189 FFVILTIYLLLKSEHNVTRSYWLIFGAGLAHGLVIHLRLYPLLFSLAYYLSL------ST 242
             V+ T+YL+ K          LI  A + +G  +H+++YP+ + L   L L        
Sbjct: 152 SLVLTTLYLIEKR---------LIACAAVFYGFAVHMKMYPVTYILPIALHLRPERDSDE 202

Query: 243 RLTQTPLDFLCQILRP-----NKQQLCLISGTLISLVAFTWTFYTMYGWEYIYEAYLYHF 297
            L      F  ++        +   L  ++   ++ +A ++ FY  YGWE++   YLYH 
Sbjct: 203 GLRLARYSFQARLYDFLKRLCSWAVLLFVAIAGLTFLALSFGFYYKYGWEFLEHTYLYHL 262

Query: 298 VRKDPRHNFSLQFLLQYLGSASSVAEPSAILKTLVLAPQFLLILYLSLSFGQFRQTLPFC 357
            R+D RHNFS  F + YL + S   + S  L      PQF+L+   + SF  +R  L FC
Sbjct: 263 TRRDIRHNFSPYFYMLYLTAES---KWSFTLGIAAFLPQFILL--SAASFAYYRD-LVFC 316

Query: 358 IFAVAFVIVTYNSVVTSQYFIWYLAILPLCLNNFKMLSWRRCFGLLFLWLLAQALWLLPA 417
            F    + VT+N V TSQYF+WYL +LPL +   +M  W+R   LL LW + QALWL PA
Sbjct: 317 CFLHTSIFVTFNKVCTSQYFLWYLCLLPLVMPLVRM-PWKRAVVLLMLWFIGQALWLAPA 375

Query: 418 YLLEFHTWNTFYWIGLQGAVFFATNGYILEQLLSHY 453
           Y+LEF   NTF +I L G  F   N  IL Q++SHY
Sbjct: 376 YVLEFQGKNTFLFIWLAGLFFLLINCSILIQIISHY 411
 Score =  129 bits (323), Expect = 9e-29
 Identities = 39/84 (46%), Positives = 54/84 (63%)

Query: 29  ISALLRIALICYGQLHDSQSAVPYTDIDYKVVTDGARQVLAGDTPFARHTYRYSPIMAYL 88
           ++ L R+AL+ YG   D    V YTDIDY V TD AR V  G +P+ R TYRY+P++++L
Sbjct: 24  VAFLARVALVFYGVFQDRTLLVRYTDIDYHVFTDAARFVTEGRSPYLRATYRYTPLLSWL 83

Query: 89  QTFNILLHPAWGKLLYATFDLLIA 112
            T N+ L   +GK L+ + DLL A
Sbjct: 84  LTPNVYLSELFGKFLFISCDLLTA 107
>emb|CAB89880.1| (AL354632) hypothetical transmembrane protein [Schizosaccharomyces
           pombe]
          Length = 815

 Score =  330 bits (839), Expect = 3e-89
 Identities = 104/285 (36%), Positives = 151/285 (52%), Gaps = 19/285 (6%)

Query: 167 FWLYNPLTAVISTRGSGDCFSSFFVILTIYLLLKSEHNVTRSYWLIFGAGLAHGLVIHLR 226
           FW+ NP  A+ISTRG+ +       I  +YL+ K      +S WL   A L  G  +H +
Sbjct: 546 FWILNPFVAIISTRGNCEAILGILSIALLYLIEK------KSVWL---ASLILGFSVHFK 596

Query: 227 LYPLLFSLAYYLSLST-RLTQTPLDFLCQILRPNKQQLCLISGTLISLVAFTWTFYTMYG 285
           +YP ++ +A+ +  S  +   T ++    +L  N  QL ++ G+L          Y +YG
Sbjct: 597 IYPFMYGIAFLVYFSKPKKGSTFMEKFLSLLSIN--QLKIVVGSLFMFTICNLLMYYLYG 654

Query: 286 WEYIYEAYLYHFVRKDPRHNFSLQFLLQYLGSASSVAEPSAILKTLVLAPQFLLILYLSL 345
             ++   YLYHF R D RHNFSL  L  Y  S+   A+ S++       PQ  L + + L
Sbjct: 655 SPFLEHTYLYHFGRTDHRHNFSLHHLNLYYESSFG-AKASSLF---AFLPQLSLCMLIPL 710

Query: 346 SFGQFRQTLPFCIFAVAFVIVTYNSVVTSQYFIWYLAILPLCLNNFKMLSWRRCFGLLFL 405
            FG+  + LP  +FA  F  VT+N V TSQYF+WYL  LPL L N K+LS ++    L L
Sbjct: 711 VFGK--KNLPGTLFAQTFAFVTFNKVCTSQYFMWYLVFLPLVLPNSKLLS-KKGLICLSL 767

Query: 406 WLLAQALWLLPAYLLEFHTWNTFYWIGLQGAVFFATNGYILEQLL 450
           W++ Q LWL+ AY LE    + F  + L G +FF  N Y L+ +L
Sbjct: 768 WIIGQLLWLISAYNLEMLGKSVFIPLWLSGLLFFFFNVYELKIIL 812
 Score =  128 bits (321), Expect = 2e-28
 Identities = 39/103 (37%), Positives = 62/103 (59%)

Query: 26  HLLISALLRIALICYGQLHDSQSAVPYTDIDYKVVTDGARQVLAGDTPFARHTYRYSPIM 85
            LL+  L+R+ L+ YG  HD +SA+ +TDIDY V +D ++ V  G +P+ R TYRY+P++
Sbjct: 438 LLLLGLLMRLVLLNYGIWHDRRSALKFTDIDYFVFSDASKYVSIGMSPYMRDTYRYTPML 497

Query: 86  AYLQTFNILLHPAWGKLLYATFDLLIATLIYRLVHMEIKSQYQ 128
           A L        P+WGK L++  DL+   L+ +L+   I  +  
Sbjct: 498 AILLLPTQYGFPSWGKYLFSISDLIAGWLMIKLLSRRISYKRS 540
>dbj|BAB20994.1| (AB050105) TbPIG-M [Trypanosoma brucei]
          Length = 430

 Score =  329 bits (835), Expect = 9e-89
 Identities = 69/291 (23%), Positives = 122/291 (41%), Gaps = 28/291 (9%)

Query: 167 FWLYNPLTAVISTRGSGDCFSSFFVILTIYLLLKSEHNVTRSYWLIFGAGLAHGLVIHLR 226
           F L+NP+   +STRG+ D   +F  ++ +    + +          + A    G  +H +
Sbjct: 124 FILFNPIVLSVSTRGNSDMLVTFMSLMVLSKFARRK---------CYQAAAVLGFAVHFK 174

Query: 227 LYPLLFSLAYYLSLSTRLTQTPLDFLCQILRPNKQQLCLISGTLISLVAFTWTFYTMYGW 286
           +YP++++L   L +  +      +   ++++             IS    T   Y  YG 
Sbjct: 175 IYPIIYALPLTLGVWEQSVAASTNTWRRVVKTAVVVSICALMAAISFAVPTVLCYMKYGQ 234

Query: 287 EYIYEAYLYHFVRKDPRHNFSLQFLLQYLGSASSVAEPSAILKTL--VLAPQFLLILYLS 344
           +Y+ EA++YH  R+D RHNFS  +LL YL  A                  PQ +++ ++S
Sbjct: 235 QYLNEAFIYHVYREDHRHNFSPYWLLMYLNMARRHLGQGVDFSPRLVAFVPQAVVLSFVS 294

Query: 345 LSFGQFRQTLPFCIFAVAFVIVTYNSVVTSQYFIWYLAILPLCL--------------NN 390
               + R+           + V +N V T QYF+W++  L                    
Sbjct: 295 Y---KLRRNTAHACCVQTVLFVAFNKVCTVQYFVWFIPFLAFLFCEPKEVEDDESGGSGA 351

Query: 391 FKMLSWRRCFGLLFLWLLAQALWLLPAYLLEFHTWNTFYWIGLQGAVFFAT 441
           FK  SW +  G++ +W     LW+  A  LEFH ++ F  + +   +FF  
Sbjct: 352 FKFFSWVKALGVVLMWAATIPLWVTTAVPLEFHGYSDFAKLWIVSCLFFLA 402
 Score =  154 bits (388), Expect = 2e-36
 Identities = 48/118 (40%), Positives = 67/118 (56%)

Query: 22  SFRTHLLISALLRIALICYGQLHDSQSAVPYTDIDYKVVTDGARQVLAGDTPFARHTYRY 81
           S +  LL+ ALLR+ LI Y   HD    V YTDIDY +V DGAR +  G +PF R T+RY
Sbjct: 11  SLQKLLLLGALLRLILIAYAFFHDQWFRVKYTDIDYMIVVDGARHMWNGGSPFDRTTFRY 70

Query: 82  SPIMAYLQTFNILLHPAWGKLLYATFDLLIATLIYRLVHMEIKSQYQKTVQHLLSKFN 139
           +P++A L   +I +    GKL++A+ DL  A   Y ++    K +  K +  L   FN
Sbjct: 71  TPLLAALVMPSIWIANPMGKLIFASSDLGAAWYCYGVLKSFAKERSAKWMVSLFILFN 128
>ref|NP_012547.1| (NC_001142) Protein required for cell viability; Yjr013wp
           [Saccharomyces cerevisiae]
 sp|P47088|YJY3_YEAST HYPOTHETICAL 35.6 KD PROTEIN IN SPC1-ILV3 INTERGENIC REGION
 pir||S55201 hypothetical protein YJR013w - yeast (Saccharomyces cerevisiae)
 emb|CAA60935.1| (X87611) ORF YJR83.11 [Saccharomyces cerevisiae]
 emb|CAA89537.1| (Z49513) ORF YJR013w [Saccharomyces cerevisiae]
          Length = 305

 Score =  321 bits (815), Expect = 2e-86
 Identities = 95/289 (32%), Positives = 141/289 (47%), Gaps = 24/289 (8%)

Query: 168 WLYNPLTAVISTRGSGDCFSSFFVILTIYLLLKSEHNVTRSYWLIFGAGLAHGLVIHLR- 226
           WL NP+   ISTRG+ +      ++ T++ L KS + +         AG+ +GL IH + 
Sbjct: 20  WLLNPMVITISTRGNAESVLCCLIMFTLFFLQKSRYTL---------AGILYGLSIHFKI 70

Query: 227 -LYPLLFSLAYYLSLSTRLTQTPLDFLCQILRPNKQQLCLISGTLISLVAFTWTFYTMYG 285
                   +A ++  + R  Q P   L  +L        +   TL +L+   W  Y +YG
Sbjct: 71  YPIIYCIPIAIFIYYNKR-NQGPRTQLTSLLN-------IGLSTLTTLLGCGWAMYKIYG 122

Query: 286 WEYIYEAYLYHFVRKDPRHNFSLQFLLQYLGSASSVAEPSAILKTLVLAPQFLLILYLSL 345
           +E++ +AYLYH  R D RHNFS+  +L YL SA+     S  L      PQ LL+L    
Sbjct: 123 YEFLDQAYLYHLYRTDHRHNFSVWNMLLYLDSANKENGESN-LSRYAFVPQLLLVLVTGC 181

Query: 346 S--FGQFRQTLPFCIFAVAFVIVTYNSVVTSQYFIWYLAILPLCLNNFKMLSWRRCFGLL 403
              +      L   +F   F  VTYN V TSQYF+WYL  LP  L+    + W++   + 
Sbjct: 182 LEWWNPTFDNLLRVLFVQTFAFVTYNKVCTSQYFVWYLIFLPFYLSR-THIGWKKGLLMA 240

Query: 404 FLWLLAQALWLLPAYLLEFHTWNTFYW-IGLQGAVFFATNGYILEQLLS 451
            LW+  Q +WL   Y LEF   N FY  + +   +FF TN ++L Q ++
Sbjct: 241 TLWVGTQGIWLSQGYYLEFEGKNVFYPGLFIASVLFFVTNVWLLGQFIT 289
>emb|CAC34506.1| (AL589883) mannosyltransferase-like protein [Arabidopsis thaliana]
          Length = 417

 Score =  300 bits (762), Expect = 3e-80
 Identities = 92/302 (30%), Positives = 142/302 (46%), Gaps = 46/302 (15%)

Query: 185 CFSSFFVILTIYLLLKSEHNVTRSYWLIFGAGLAHGLVIHLRLYPLLFSLAYYLSLSTRL 244
           CFS     ++++ +  S  N+ +       A   +GLV+H R+YP++++L   L L T++
Sbjct: 113 CFSVVLESISMFQIYWSAGNLLQ-------AAFWYGLVVHFRVYPIIYALPIILVLDTQV 165

Query: 245 ----------------TQTP-------------LDFLCQILRPNKQQLCLISGTLISLVA 275
                            +TP             L  L  +    +    LISG +   +A
Sbjct: 166 FRSGQKPALLYWNTGQAKTPASNMERKTFLFNLLTTLKSLFSRERIMFALISGGV--FLA 223

Query: 276 FTWTFYTMYGWEYIYEAYLYHFVRKDPRHNFSLQFLLQYLGSASSVAEPSAILKTLVLAP 335
                +  YG E+++EA LYH  R DPRHNFS+ F   YL         SA+ K +   P
Sbjct: 224 CNAVSFYFYGQEFLHEALLYHLTRTDPRHNFSIYFYHIYLHYERQF---SAVEKLISFLP 280

Query: 336 QFLLILYLSLSFGQFRQTLPFCIFAVAFVIVTYNSVVTSQYFIWYLAILPLCLNNFKM-L 394
           QF +   L   F    Q L FCIF      VT+N V+T+QYF+W+  +LPL L    M L
Sbjct: 281 QFTVQFALVFCFS---QDLVFCIFLQTVAFVTFNKVITAQYFVWFYCLLPLILPWSHMKL 337

Query: 395 SWRRCFGLLFLWLLAQALWLLPAYLLEFHTWNTFYWIGLQGAVFFATNGYILEQLLSHYG 454
            W     ++ +W+ AQ  WLL  Y+LEF   N F  + +   +F A N ++L +++  + 
Sbjct: 338 KWEGLLCII-MWIGAQTHWLLWGYMLEFKGVNVFLPLWIASLLFLAANTFVLVRIIQRHR 396

Query: 455 FT 456
           F+
Sbjct: 397 FS 398
 Score =  300 bits (762), Expect = 3e-80
 Identities = 92/302 (30%), Positives = 142/302 (46%), Gaps = 46/302 (15%)

Query: 185 CFSSFFVILTIYLLLKSEHNVTRSYWLIFGAGLAHGLVIHLRLYPLLFSLAYYLSLSTRL 244
           CFS     ++++ +  S  N+ +       A   +GLV+H R+YP++++L   L L T++
Sbjct: 113 CFSVVLESISMFQIYWSAGNLLQ-------AAFWYGLVVHFRVYPIIYALPIILVLDTQV 165

Query: 245 ----------------TQTP-------------LDFLCQILRPNKQQLCLISGTLISLVA 275
                            +TP             L  L  +    +    LISG +   +A
Sbjct: 166 FRSGQKPALLYWNTGQAKTPASNMERKTFLFNLLTTLKSLFSRERIMFALISGGV--FLA 223

Query: 276 FTWTFYTMYGWEYIYEAYLYHFVRKDPRHNFSLQFLLQYLGSASSVAEPSAILKTLVLAP 335
                +  YG E+++EA LYH  R DPRHNFS+ F   YL         SA+ K +   P
Sbjct: 224 CNAVSFYFYGQEFLHEALLYHLTRTDPRHNFSIYFYHIYLHYERQF---SAVEKLISFLP 280

Query: 336 QFLLILYLSLSFGQFRQTLPFCIFAVAFVIVTYNSVVTSQYFIWYLAILPLCLNNFKM-L 394
           QF +   L   F    Q L FCIF      VT+N V+T+QYF+W+  +LPL L    M L
Sbjct: 281 QFTVQFALVFCFS---QDLVFCIFLQTVAFVTFNKVITAQYFVWFYCLLPLILPWSHMKL 337

Query: 395 SWRRCFGLLFLWLLAQALWLLPAYLLEFHTWNTFYWIGLQGAVFFATNGYILEQLLSHYG 454
            W     ++ +W+ AQ  WLL  Y+LEF   N F  + +   +F A N ++L +++  + 
Sbjct: 338 KWEGLLCII-MWIGAQTHWLLWGYMLEFKGVNVFLPLWIASLLFLAANTFVLVRIIQRHR 396

Query: 455 FT 456
           F+
Sbjct: 397 FS 398
 Score =  131 bits (327), Expect = 3e-29
 Identities = 39/88 (44%), Positives = 57/88 (64%)

Query: 20  YTSFRTHLLISALLRIALICYGQLHDSQSAVPYTDIDYKVVTDGARQVLAGDTPFARHTY 79
           +  FR+ L+ S LLR+ LI YG+  D+   V YTD+DY V +D A  + +G++P+ R TY
Sbjct: 15  WMKFRSLLVFSMLLRVFLIVYGEWQDAHMEVRYTDVDYIVFSDAASLMASGESPYKRTTY 74

Query: 80  RYSPIMAYLQTFNILLHPAWGKLLYATF 107
           RYSP++A L T N   H +WGK L++  
Sbjct: 75  RYSPLLALLLTPNSFFHRSWGKFLFSAS 102
>dbj|BAA87228.1| (AB027924) Hypothetical protein [Schizosaccharomyces pombe]
          Length = 149

 Score =  145 bits (365), Expect = 1e-33
 Identities = 42/136 (30%), Positives = 67/136 (48%), Gaps = 12/136 (8%)

Query: 167 FWLYNPLTAVISTRGSGDCFSSFFVILTIYLLLKSEHNVTRSYWLIFGAGLAHGLVIHLR 226
           FW+ NP  A+ISTRG+ +       I  +YL+ K      +S WL   A L  G  +H +
Sbjct: 25  FWILNPFVAIISTRGNCEAILGILSIALLYLIEK------KSVWL---ASLILGFSVHFK 75

Query: 227 LYPLLFSLAYYLSLST-RLTQTPLDFLCQILRPNKQQLCLISGTLISLVAFTWTFYTMYG 285
           +YP ++ +A+ +  S  +   T ++    +L  N  QL ++ G+L          Y +YG
Sbjct: 76  IYPFMYGIAFLVYFSKPKKGSTFMEKFLSLLSIN--QLKIVVGSLFMFTICNLLMYYLYG 133

Query: 286 WEYIYEAYLYHFVRKD 301
             ++   YLYHF R D
Sbjct: 134 SPFLEHTYLYHFGRTD 149
>pir||T36589 probable transmembrane protein - Streptomyces coelicolor
 emb|CAB42730.1| (AL049826) putative transmembrane protein [Streptomyces coelicolor
           A3(2)]
          Length = 502

 Score = 38.9 bits (90), Expect = 0.20
 Identities = 36/237 (15%), Positives = 68/237 (28%), Gaps = 39/237 (16%)

Query: 216 GLAHGLVIHLRLYPLLFSLAYYLSLST--RLTQTPLDFLCQILRPNKQQLCLISGTLISL 273
           G+  GL    +LYP L      +      +                   L ++       
Sbjct: 224 GVLLGLATAAKLYPFLILGPLLVLCWRAGKWRAFATALGGAAGAWLVVNLPVML-----F 278

Query: 274 VAFTWTFYTMYGWEYIYEAYLYHFVRKDPRHNFSLQFLLQYLGSASSVAEPSAILKTLVL 333
               W+ +  +  E                 +F   +L+    S +  +     + TL  
Sbjct: 279 AFDGWSKFYTFSQE--------------RGVDFGSFWLILVQNSDNPPSTE--TVNTLAT 322

Query: 334 APQFLLILYLSL--SFGQFRQTLPFCIFAVAFVIVTYNSVVTSQYFIWYLAILPLCLNNF 391
               L    ++        R       F +    +  N V + QY +W + +  L    +
Sbjct: 323 LLMLLCCGAVAALTLTAPRRPRFAQLAFLIVAAFILTNKVYSPQYVLWLVPLAALARPKW 382

Query: 392 K-MLSWRRCFGLLFLWLLAQALWLLPAYL--------LEFHTWNTFYWIGLQGAVFF 439
           +  L W+ C    FL      +W+  AY         L    ++    + L G +F 
Sbjct: 383 RDFLIWQACEVAYFL-----GIWMYLAYTTSGDAHKGLPTDGYHWAIGLHLLGTLFL 434
CPU time:   132.00 user secs.	    2.20 sys. secs	  134.20 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.321    0.170    0.548 

Gapped
Lambda     K      H
   0.270   0.0597    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 322905181
Number of Sequences: 887402
Number of extensions: 16424397
Number of successful extensions: 64002
Number of sequences better than 10.0: 172
Number of HSP's better than 10.0 without gapping: 16
Number of HSP's successfully gapped in prelim test: 158
Number of HSP's that attempted gapping in prelim test: 63782
Number of HSP's gapped (non-prelim): 273
length of query: 466
length of database: 277,845,442
effective HSP length: 51
effective length of query: 415
effective length of database: 232,587,940
effective search space: 96523995100
effective search space used: 96523995100
T: 11
A: 40
X1: 15 ( 6.9 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (21.1 bits)
S2: 75 (33.3 bits)