Sequences with E-value BETTER than threshold (0.002) 317 9e-86
262 3e-69
185 4e-46
178 5e-44
163 2e-39
154 1e-36
138 8e-32
Sequences with E-value WORSE than threshold gb|AAF54173.1| (AE003677) CG14463 gene product [Drosophila melan... 39 0.054
ref|NP_014360.1| (NC_001146) Glysosyl Phosphatidyl Inositol; Gpi... 33 4.8
ref|NP_326456.1| (NC_002771) ABC TRANSPORTER ATP-BINDING AND PER... 32 9.2
Alignments
>ref|NP_004560.1| (NM_004569) phosphatidylinositol glycan, class H [Homo sapiens]
pir||A48024 glycosylphosphatidylinositol anchor class H biosynthesis protein -
human
gb|AAA03545.1| (L19783) GPI-H [Homo sapiens]
gb|AAH04100.1|AAH04100 (BC004100) phosphatidylinositol glycan, class H [Homo sapiens]
Length = 188
Score = 317 bits (805), Expect = 9e-86
Identities = 188/188 (100%), Positives = 188/188 (100%)
Query: 1 MEDERSFSDICGGRLALQRRYYSPSCREFCLSCPRLSLRSLTAVTCTVWLAAYGLFTLCE 60
MEDERSFSDICGGRLALQRRYYSPSCREFCLSCPRLSLRSLTAVTCTVWLAAYGLFTLCE
Sbjct: 1 MEDERSFSDICGGRLALQRRYYSPSCREFCLSCPRLSLRSLTAVTCTVWLAAYGLFTLCE 60
Query: 61 NSMILSAAIFITLLGLLGYLHFVKIDQETLLIIDSLGIQMTSSYASGKESTTFIEMGKVK 120
NSMILSAAIFITLLGLLGYLHFVKIDQETLLIIDSLGIQMTSSYASGKESTTFIEMGKVK
Sbjct: 61 NSMILSAAIFITLLGLLGYLHFVKIDQETLLIIDSLGIQMTSSYASGKESTTFIEMGKVK 120
Query: 121 DIVINEAIYMQKVIYYLCILLKDPVEPHGISQVVPVFQSAKPRLDCLIEVYRSCQEILAH 180
DIVINEAIYMQKVIYYLCILLKDPVEPHGISQVVPVFQSAKPRLDCLIEVYRSCQEILAH
Sbjct: 121 DIVINEAIYMQKVIYYLCILLKDPVEPHGISQVVPVFQSAKPRLDCLIEVYRSCQEILAH 180
Query: 181 QKATSTSP 188
QKATSTSP
Sbjct: 181 QKATSTSP 188
>dbj|BAB32246.1| (AK020902) data source:SPTR, source key:Q14442,
evidence:ISS~homolog to PHOSPHATIDYLINOSITOL GLYCAN,
CLASS H~putative [Mus musculus]
Length = 167
Score = 262 bits (665), Expect = 3e-69
Identities = 140/158 (88%), Positives = 147/158 (92%)
Query: 1 MEDERSFSDICGGRLALQRRYYSPSCREFCLSCPRLSLRSLTAVTCTVWLAAYGLFTLCE 60
MEDE+SFSDICGGRLAL+ RYYSP CREF LS RLSL SLTAVTC VWLAAYGLFTLCE
Sbjct: 1 MEDEKSFSDICGGRLALRCRYYSPYCREFGLSSARLSLCSLTAVTCAVWLAAYGLFTLCE 60
Query: 61 NSMILSAAIFITLLGLLGYLHFVKIDQETLLIIDSLGIQMTSSYASGKESTTFIEMGKVK 120
NSM+LSA IFIT+LGLLGYLHFVKIDQETLLIIDSLGIQMTSSYASGKESTTFIEM KVK
Sbjct: 61 NSMVLSATIFITILGLLGYLHFVKIDQETLLIIDSLGIQMTSSYASGKESTTFIEMDKVK 120
Query: 121 DIVINEAIYMQKVIYYLCILLKDPVEPHGISQVVPVFQ 158
DI+INEAIYMQKVIYYLCILLK+P +PH ISQVVPVFQ
Sbjct: 121 DIIINEAIYMQKVIYYLCILLKEPGKPHEISQVVPVFQ 158
>gb|AAL62432.1| (AY072440) putative protein [Arabidopsis thaliana]
Length = 195
Score = 185 bits (467), Expect = 4e-46
Identities = 39/140 (27%), Positives = 71/140 (49%), Gaps = 9/140 (6%)
Query: 48 VWLAAYGLFTLCENSMILSAAIFITLLGLLGYLHFVK-IDQETLLIIDSLGIQMTSSYAS 106
V+LA+ F L +++ + + L G L LH K + +E+++I+ + GIQ+ + Y S
Sbjct: 52 VFLASSMYFLLGKDNPARTLSWGCLLSGFLVMLHSRKFVKKESVIILPTFGIQLETQYLS 111
Query: 107 GKESTTFIEMGKVKDIVINEAIYMQKVIYYLCILLKDPVEPHGISQVVPVFQSAKPRLDC 166
GK + FI + K+ V+ E + + L + L+ G Q+ VF+ +P L
Sbjct: 112 GKTVSRFIPIDKILKPVLVECVTPITCYWSLSLFLR------GEEQLTLVFKELRPPLKM 165
Query: 167 LIEVYRS-CQEI-LAHQKAT 184
L+ ++++ C I HQ T
Sbjct: 166 LVPIWKALCAAIGTDHQSET 185
>ref|NP_195278.1| (NM_119718) putative protein [Arabidopsis thaliana]
pir||T04658 hypothetical protein F8D20.40 - Arabidopsis thaliana
emb|CAA20023.1| (AL031135) putative protein [Arabidopsis thaliana]
emb|CAB80269.1| (AL161587) putative protein [Arabidopsis thaliana]
Length = 204
Score = 178 bits (449), Expect = 5e-44
Identities = 39/149 (26%), Positives = 71/149 (47%), Gaps = 18/149 (12%)
Query: 48 VWLAAYGLFTLCE---------NSMILSAAIFITLLGLLGYLHFVK-IDQETLLIIDSLG 97
V+LA+ F L + ++ + + L G L LH K + +E+++I+ + G
Sbjct: 52 VFLASSMYFLLGKLKGIIHMKQDNPARTLSWGCLLSGFLVMLHSRKFVKKESVIILPTFG 111
Query: 98 IQMTSSYASGKESTTFIEMGKVKDIVINEAIYMQKVIYYLCILLKDPVEPHGISQVVPVF 157
IQ+ + Y SGK + FI + K+ V+ E + + L + L+ G Q+ VF
Sbjct: 112 IQLETQYLSGKTVSRFIPIDKILKPVLVECVTPITCYWSLSLFLR------GEEQLTLVF 165
Query: 158 QSAKPRLDCLIEVYRS-CQEI-LAHQKAT 184
+ +P L L+ ++++ C I HQ T
Sbjct: 166 KELRPPLKMLVPIWKALCAAIGTDHQSET 194
>ref|XP_018042.2| (XM_018042) similar to phosphatidylinositol glycan, class H (H.
sapiens) [Homo sapiens]
Length = 92
Score = 163 bits (409), Expect = 2e-39
Identities = 87/90 (96%), Positives = 88/90 (97%)
Query: 1 MEDERSFSDICGGRLALQRRYYSPSCREFCLSCPRLSLRSLTAVTCTVWLAAYGLFTLCE 60
MEDERSFSDICGGRLALQRRYY PSCREFCLSCP LSLRSLTAVTCTVWLAAYGLFTLCE
Sbjct: 1 MEDERSFSDICGGRLALQRRYYFPSCREFCLSCPGLSLRSLTAVTCTVWLAAYGLFTLCE 60
Query: 61 NSMILSAAIFITLLGLLGYLHFVKIDQETL 90
NSMILSAAIFITLLGLLGYLHFVKIDQET+
Sbjct: 61 NSMILSAAIFITLLGLLGYLHFVKIDQETV 90
>pir||T40636 glycosylphosphatidylinositol anchor protein homolog - fission yeast
(Schizosaccharomyces pombe)
emb|CAB39362.1| (AL049474) similar to glycosylphosphatidylinositol anchor
[Schizosaccharomyces pombe]
Length = 160
Score = 154 bits (386), Expect = 1e-36
Identities = 45/147 (30%), Positives = 68/147 (45%), Gaps = 22/147 (14%)
Query: 52 AYG--LFTLCENSMILSAA---------IFITLLG---LLGYLHFVK-IDQETLLIIDSL 96
+YG +F LC S ++ A I ITL+ LL H + ++ E+L +I L
Sbjct: 20 SYGTQMFALCFISFVIGATSLAIGRSPKIIITLVELSFLLSLFHIISGVNHESLFVIRDL 79
Query: 97 GIQMT-SSYASGKESTTFIEMGKVKDIVINEAIYMQKVIYYLCILLKDPVEPHGISQVVP 155
G+Q S K S+ I + ++DI INE V YY+ I ++ E H
Sbjct: 80 GVQTNCHSIVPWKSSSKLIPLDSIRDIFINEGFRKFDVCYYMGIAIESETEIH------V 133
Query: 156 VFQSAKPRLDCLIEVYRSCQEILAHQK 182
VF + PR D L +VY+ +LA+
Sbjct: 134 VFPTLLPRHDVLQKVYKETVILLANNS 160
>emb|CAD21200.1| (AL669999) conserved hypothetical protein [Neurospora crassa]
Length = 263
Score = 138 bits (345), Expect = 8e-32
Identities = 38/125 (30%), Positives = 59/125 (46%), Gaps = 20/125 (16%)
Query: 64 ILSAAIFITLLGLLGYLHFVKIDQETLLIIDSLGIQMTSSYASGK----------ESTTF 113
+L+ + + + L +H E+LL++ LGIQM+SS G + T F
Sbjct: 114 VLALSALTSYIALFARIH----TTESLLVLRGLGIQMSSSVGGGNFFRLGGGTFMKRTRF 169
Query: 114 IEMGKVKDIVINEAIYMQKVIYYLCILLKDPVEPHGISQVVPVFQSAKPRLDCLIEVYRS 173
I K++DI+INEA +V YYL I+++ G VV F PR + V+R
Sbjct: 170 IPTEKIQDILINEAFKGFEVRYYLVIVVE------GEQDVVVCFPRLLPRRKIVERVWRG 223
Query: 174 CQEIL 178
+ L
Sbjct: 224 ARGCL 228
>gb|AAF54173.1| (AE003677) CG14463 gene product [Drosophila melanogaster]
Length = 221
Score = 38.9 bits (90), Expect = 0.054
Identities = 27/96 (28%), Positives = 45/96 (46%), Gaps = 2/96 (2%)
Query: 76 LLGYLHFVKIDQETLLIIDSLGIQMTSSYASGKESTTFIEMGKVKDIVINEAIYMQKVIY 135
LL + E L + +QM + + G+ES ++ G ++DIV+NE I V Y
Sbjct: 99 LLIRSTLNLVQAERLFYSWDMALQMETVRSFGRESVLCVQRGHIEDIVLNEVIEDLDVKY 158
Query: 136 YLCILLKDPVEPHGISQVVPVFQSAKPRLDCLIEVY 171
L +L+ ++P+F S P ++CL Y
Sbjct: 159 ML--ILRTKGSQFKKRPIIPLFNSQSPSIECLQHTY 192
>ref|NP_014360.1| (NC_001146) Glysosyl Phosphatidyl Inositol; Gpi15p [Saccharomyces
cerevisiae]
sp|P53961|YND8_YEAST HYPOTHETICAL 24.7 KD PROTEIN IN TFC5-IDH1 INTERGENIC REGION
pir||S62960 probable membrane protein YNL038w - yeast (Saccharomyces
cerevisiae)
emb|CAA95905.1| (Z71314) ORF YNL038w [Saccharomyces cerevisiae]
Length = 212
Score = 32.7 bits (74), Expect = 4.8
Identities = 20/85 (23%), Positives = 34/85 (39%), Gaps = 11/85 (12%)
Query: 69 IFITLLGLLGYLHFVKIDQETLLIIDSLGIQMTSSYA------SGK----ESTTFIEMGK 118
I + L L + ET+ I G+Q++ E FI +
Sbjct: 103 IIMGLFALGTIILVRGPSVETVTIFKESGLQLSRVKGMVIFPQQWNRKFFEQVEFISNER 162
Query: 119 VKDIVINEAI-YMQKVIYYLCILLK 142
+ D+VINE +VI+YL +++
Sbjct: 163 IIDVVINEGFCRGFRVIFYLAAIVR 187
>ref|NP_326456.1| (NC_002771) ABC TRANSPORTER ATP-BINDING AND PERMEASE PROTEIN
[Mycoplasma pulmonis]
emb|CAC13798.1| (AL445565) ABC TRANSPORTER ATP-BINDING AND PERMEASE PROTEIN
[Mycoplasma pulmonis]
Length = 750
Score = 31.5 bits (71), Expect = 9.2
Identities = 15/65 (23%), Positives = 29/65 (44%)
Query: 34 PRLSLRSLTAVTCTVWLAAYGLFTLCENSMILSAAIFITLLGLLGYLHFVKIDQETLLII 93
P LRS + V L + F + S++L +IF + + V ++ ++I+
Sbjct: 597 PDWELRSNAKIYANVLLGSSLSFNIAIISIMLLTSIFFLIQLIFSTKLIVNSKKKEMIIL 656
Query: 94 DSLGI 98
S G+
Sbjct: 657 KSFGV 661
CPU time: 39.66 user secs. 1.60 sys. secs 41.26 total secs.
Database: nr
Posted date: Apr 21, 2002 2:19 PM
Number of letters in database: 277,845,442
Number of sequences in database: 887,402
Lambda K H
0.320 0.163 0.482
Gapped
Lambda K H
0.270 0.0572 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 98239453
Number of Sequences: 887402
Number of extensions: 3577362
Number of successful extensions: 12848
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 12828
Number of HSP's gapped (non-prelim): 16
length of query: 188
length of database: 277,845,442
effective HSP length: 53
effective length of query: 135
effective length of database: 230,813,136
effective search space: 31159773360
effective search space used: 31159773360
T: 11
A: 40
X1: 15 ( 6.9 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (21.1 bits)
S2: 71 (31.8 bits)
