Sequences with E-value BETTER than threshold (0.002) 294 9e-79
203 2e-51
200 2e-50
200 2e-50
194 1e-48
178 7e-44
125 7e-28
124 1e-27
124 1e-27
106 3e-22
106 4e-22
100 3e-20
82 8e-15
Alignments
>ref|NP_010588.1| (NC_001136) Glycosylphosphatidylinositol (GPI) assembly; Gpi11p
[Saccharomyces cerevisiae]
pir||S61188 probable membrane protein YDR302w - yeast (Saccharomyces
cerevisiae)
gb|AAB64738.1| (U28374) Ydr302wp [Saccharomyces cerevisiae]
Length = 219
Score = 294 bits (746), Expect = 9e-79
Identities = 219/219 (100%), Positives = 219/219 (100%)
Query: 1 MPAKKRTRKTVKKTVSFSDDTTLTTHQNREKKNVDHDRPPVYVRKTPLMTFPYHLVALLY 60
MPAKKRTRKTVKKTVSFSDDTTLTTHQNREKKNVDHDRPPVYVRKTPLMTFPYHLVALLY
Sbjct: 1 MPAKKRTRKTVKKTVSFSDDTTLTTHQNREKKNVDHDRPPVYVRKTPLMTFPYHLVALLY 60
Query: 61 YYVFVSSNFNTVKLLSFLIPTQVAYLVLQFNKCTVYGNKIIKINYSLTIICLGVTFLLSF 120
YYVFVSSNFNTVKLLSFLIPTQVAYLVLQFNKCTVYGNKIIKINYSLTIICLGVTFLLSF
Sbjct: 61 YYVFVSSNFNTVKLLSFLIPTQVAYLVLQFNKCTVYGNKIIKINYSLTIICLGVTFLLSF 120
Query: 121 PTMLLTILFGAPLMDLLWETWLLSLHFAFLAYPAVYSVFNCDFKVGLWKKYFIFIVVGGW 180
PTMLLTILFGAPLMDLLWETWLLSLHFAFLAYPAVYSVFNCDFKVGLWKKYFIFIVVGGW
Sbjct: 121 PTMLLTILFGAPLMDLLWETWLLSLHFAFLAYPAVYSVFNCDFKVGLWKKYFIFIVVGGW 180
Query: 181 ISCVVIPLDWDRDWQNWPIPIVVGGYLGALVGYTIGAYI 219
ISCVVIPLDWDRDWQNWPIPIVVGGYLGALVGYTIGAYI
Sbjct: 181 ISCVVIPLDWDRDWQNWPIPIVVGGYLGALVGYTIGAYI 219
>ref|NP_002634.1| (NM_002643) phosphatidylinositol glycan, class F [Homo sapiens]
sp|Q07326|PIGF_HUMAN Phosphatidylinositol-glycan biosynthesis, class F protein (PIG-F)
pir||A46097 GPI-anchor biosynthesis protein PIG-F - human
dbj|BAA02697.1| (D13435) PIG-F [Homo sapiens]
Length = 219
Score = 203 bits (513), Expect = 2e-51
Identities = 50/188 (26%), Positives = 79/188 (41%), Gaps = 27/188 (14%)
Query: 44 RKTPLMTFPYHLVALLYYYVFVSSNFNTVKLLSFLIPTQVAYLVLQFNKCTVYGNKIIKI 103
+ + L L + S V L V YLV++ N + + K+
Sbjct: 26 IPSLFLENFSILETHLTWLCICSGFVTAVNL--------VLYLVVKPNTSSKRSSLSHKV 77
Query: 104 NYSLTIICLGVTFLLS-FPTMLLTILFGAPLMDLLWETWLLSLHFA-FLAYP-------- 153
L + FL+S F ++ +L+GAPL++L ET+L ++ + F P
Sbjct: 78 TGFLKCC---IYFLMSCFSFHVIFVLYGAPLIELALETFLFAVILSTFTTVPCLCLLGPN 134
Query: 154 --AVYSVFNCDFKVGLWKKYF----IFIVVGGWISCVVIPLDWDRDWQNWPIPIVVGGYL 207
A VF+ + +W+ I VG W+ + IPLDW+R WQ WPI +G
Sbjct: 135 LKAWLRVFSRNGVTSIWENSLQITTISSFVGAWLGALPIPLDWERPWQVWPISCTLGATF 194
Query: 208 GALVGYTI 215
G + G I
Sbjct: 195 GYVAGLVI 202
>pir||T40997 probable short-chain dehydrogenase - fission yeast
(Schizosaccharomyces pombe)
emb|CAB40182.1| (AL049559) putative short-chain dehydrogenase [Schizosaccharomyces
pombe]
Length = 503
Score = 200 bits (506), Expect = 2e-50
Identities = 47/189 (24%), Positives = 72/189 (37%), Gaps = 26/189 (13%)
Query: 44 RKTPLMTFPYHLVALLYYYVFVSSNFNTVKLLSFLIPTQVAYLVLQFNKCTVYGNKIIKI 103
T L+TF + L + N ++L P LQ + K
Sbjct: 321 IFTLLLTFTQLTIFYLSLNCLIE---NPYRMLRNTFPIWFIMQTLQIYIQSPRPPLTPKR 377
Query: 104 NYSLTIICLGVTFLLSFPTMLLTILFGAPLMDLLWETWLLSLHFA-FLAYPAVYSVFNCD 162
+ L + L+SF + + FGAPL+ T+ +L + F YP ++
Sbjct: 378 LLAGAASMLIGSLLISF----ILVAFGAPLLHDFHLTYFCALTLSVFTVYPLASTL---A 430
Query: 163 FKVGLWKKYFIFI---------------VVGGWISCVVIPLDWDRDWQNWPIPIVVGGYL 207
F W+++ ++G W IPLDWDR WQ WPI IV+G +L
Sbjct: 431 FNTEQWQRFLTLKSFNVIGSMQLRSWGPIIGAWFGAFPIPLDWDRPWQAWPITIVIGAFL 490
Query: 208 GALVGYTIG 216
G +G
Sbjct: 491 GYAFAAIVG 499
>gb|AAF18502.1|AC010924_15 (AC010924) ESTs gb|AI992787, gb|T20398 come from this gene.
[Arabidopsis thaliana]
Length = 270
Score = 200 bits (505), Expect = 2e-50
Identities = 42/175 (24%), Positives = 69/175 (39%), Gaps = 21/175 (12%)
Query: 41 VYVRKTPLMTFPYHLVALLYYYVFVSSNFNTVKLLSFLIPTQVAYLVLQFNKCTVYGNKI 100
VYV + F +H+V Y +S T++LL + V + F + +
Sbjct: 26 VYVITGLFLIFGFHVVRNKYSVDLISDPTLTLRLLWIIEFPIVVIIYSLFRRNPEKCSYF 85
Query: 101 IKINYSLTIICLGVTFLLSFPTMLLTILFGAPL-MDLLWETWLLSLHFA-FLAYPA---- 154
+ SL + G + L + GAP+ M L +T S + F PA
Sbjct: 86 RAVGRSLVGLIAG-----ALINALGAVSLGAPIGMQSLSKTIHWSFLMSVFTVVPATAVL 140
Query: 155 ------VYSVFNCDFKVGLWKKYFI----FIVVGGWISCVVIPLDWDRDWQNWPI 199
+ +F +G+ + + ++GGW +PLDW+R WQ WPI
Sbjct: 141 GASWIDWHRIFASLKPIGIIEHMLLVPAYGAIIGGWFGAWPMPLDWERPWQEWPI 195
>ref|NP_032864.1| (NM_008838) phosphatidylinositol glycan, class F [Mus musculus]
dbj|BAA08818.1| (D50264) phosphatidylinositol glycan class F [Mus musculus]
Length = 219
Score = 194 bits (489), Expect = 1e-48
Identities = 53/188 (28%), Positives = 84/188 (44%), Gaps = 27/188 (14%)
Query: 44 RKTPLMTFPYHLVALLYYYVFVSSNFNTVKLLSFLIPTQVAYLVLQFNKCTVYGNKIIKI 103
+ + L A L + S++ TV LLS YLV++ N + + K+
Sbjct: 26 IPSFFVDNFSVLEAHLTWLCICSASVTTVNLLS--------YLVVKPNVSSKRSSLSHKV 77
Query: 104 NYSLTIICLGVTFLLS-FPTMLLTILFGAPLMDLLWETWLLSLHFA-FLAYP-------- 153
+L V FL+S F ++ +L+GAPL++L+ ET+L ++ + F P
Sbjct: 78 TRALKCC---VCFLMSCFLLHIIFVLYGAPLIELVLETFLFAVVLSTFTTVPCLCLLGPN 134
Query: 154 --AVYSVFNCDFKVGLWKKYF----IFIVVGGWISCVVIPLDWDRDWQNWPIPIVVGGYL 207
A VF+ + +W+ I G W+ IPLDW+R WQ WPI +G
Sbjct: 135 LKAWLRVFSRNGVTSIWENSLQITTISSFTGAWLGAFPIPLDWERPWQVWPISCTLGATF 194
Query: 208 GALVGYTI 215
G + G I
Sbjct: 195 GYVAGLVI 202
>gb|AAH21725.1|AAH21725 (BC021725) Similar to phosphatidylinositol glycan, class F [Homo
sapiens]
Length = 206
Score = 178 bits (449), Expect = 7e-44
Identities = 43/168 (25%), Positives = 70/168 (41%), Gaps = 27/168 (16%)
Query: 44 RKTPLMTFPYHLVALLYYYVFVSSNFNTVKLLSFLIPTQVAYLVLQFNKCTVYGNKIIKI 103
+ + L L + S V L V YLV++ N + + K+
Sbjct: 26 IPSLFLENFSILETHLTWLCICSGFVTAVNL--------VLYLVVKPNTSSKRSSLSHKV 77
Query: 104 NYSLTIICLGVTFLLS-FPTMLLTILFGAPLMDLLWETWLLSLHFA-FLAYP-------- 153
L + FL+S F ++ +L+GAPL++L ET+L ++ + F P
Sbjct: 78 TGFLKCC---IYFLMSCFSFHVIFVLYGAPLIELALETFLFAVILSTFTTVPCLCLLGPN 134
Query: 154 --AVYSVFNCDFKVGLWKKYF----IFIVVGGWISCVVIPLDWDRDWQ 195
A VF+ + +W+ I VG W+ + IPLDW+R WQ
Sbjct: 135 LKAWLRVFSRNGVTSIWENSLQITTISSFVGAWLGALPIPLDWERPWQ 182
>gb|AAL48412.1| (AY070790) AT13969p [Drosophila melanogaster]
Length = 236
Score = 125 bits (312), Expect = 7e-28
Identities = 37/116 (31%), Positives = 56/116 (47%), Gaps = 19/116 (16%)
Query: 116 FLLSFPTMLLTILFGAPLMDLLWETWLLSLHFAFLAY-PAVY-----------------S 157
FL + + I+ GAP++ +T++L+L L P V+
Sbjct: 99 FLCTLLYAFICIILGAPVLGNYEQTFVLALLMTLLTVSPTVFLLGGGGALQVCFCEKPDF 158
Query: 158 VFNC-DFKVGLWKKYFIFIVVGGWISCVVIPLDWDRDWQNWPIPIVVGGYLGALVG 212
V C D + L+K + ++G W VV PLDW RDWQ +PIP V+G LG+ +G
Sbjct: 159 VTKCEDTALNLFKYNALGGILGAWAGSVVAPLDWGRDWQAYPIPNVIGALLGSALG 214
>gb|AAF49133.1| (AE003516) CG9376 gene product [Drosophila melanogaster]
Length = 209
Score = 124 bits (310), Expect = 1e-27
Identities = 37/116 (31%), Positives = 56/116 (47%), Gaps = 19/116 (16%)
Query: 116 FLLSFPTMLLTILFGAPLMDLLWETWLLSLHFAFLAY-PAVY-----------------S 157
FL + + I+ GAP++ +T++L+L L P V+
Sbjct: 72 FLCTLLYAFICIILGAPVLGNYEQTFVLALLMTLLTVSPTVFLLGGGGALQVCFCEKPDF 131
Query: 158 VFNC-DFKVGLWKKYFIFIVVGGWISCVVIPLDWDRDWQNWPIPIVVGGYLGALVG 212
V C D + L+K + ++G W VV PLDW RDWQ +PIP V+G LG+ +G
Sbjct: 132 VTKCEDTALNLFKYNALGGILGAWAGSVVAPLDWGRDWQAYPIPNVIGALLGSALG 187
>ref|XP_018442.1| (XM_018442) similar to phosphatidylinositol glycan, class F (H.
sapiens) [Homo sapiens]
Length = 153
Score = 124 bits (310), Expect = 1e-27
Identities = 31/137 (22%), Positives = 54/137 (38%), Gaps = 23/137 (16%)
Query: 44 RKTPLMTFPYHLVALLYYYVFVSSNFNTVKLLSFLIPTQVAYLVLQFNKCTVYGNKIIKI 103
+ + L L + S V L V YLV++ N + + K+
Sbjct: 26 IPSLFLENSSILETHLTWLCVCSGFVTAVSL--------VLYLVVKPNTSSKISSLSYKV 77
Query: 104 NYSLTIICLGVTFLLS-FPTMLLTILFGAPLMDLLWETWLLSLHFA-FLAYP-------- 153
L + FL+S F ++ +L+GAPL++L ET+L ++ + F P
Sbjct: 78 TRFLKCC---IYFLMSCFSFHVIFVLYGAPLIELALETFLFAVILSTFTIGPYLCLLGPN 134
Query: 154 --AVYSVFNCDFKVGLW 168
A VF+ + +W
Sbjct: 135 LKAWLRVFSRNGVTSIW 151
>pir||T29643 hypothetical protein F49E8.1 - Caenorhabditis elegans
Length = 571
Score = 106 bits (264), Expect = 3e-22
Identities = 33/106 (31%), Positives = 51/106 (47%), Gaps = 18/106 (16%)
Query: 122 TMLLTILFGAPLM-DLLWETWLLSLHFAFLAYPAVY-------------SVFNCDFKVGL 167
+L +LFGAP D++ L + A A PAV+ +F+C+
Sbjct: 18 FYILAVLFGAPFFSDIIATAVLATALTAVSALPAVFLFDSEERAVEVILQLFSCEGNPTP 77
Query: 168 WKKYFI----FIVVGGWISCVVIPLDWDRDWQNWPIPIVVGGYLGA 209
+ + F +G W + V PLDWDR WQ +P+P +VG ++GA
Sbjct: 78 KESVLLFNSVFAFLGAWAAAAVHPLDWDRWWQRYPLPSLVGCFIGA 123
>ref|NP_501226.1| (NM_068825) F49E8.1.p [Caenorhabditis elegans]
gb|AAB03156.2| (U61949) Hypothetical protein F49E8.1 [Caenorhabditis elegans]
Length = 553
Score = 106 bits (263), Expect = 4e-22
Identities = 33/106 (31%), Positives = 51/106 (47%), Gaps = 18/106 (16%)
Query: 122 TMLLTILFGAPLM-DLLWETWLLSLHFAFLAYPAVY-------------SVFNCDFKVGL 167
+L +LFGAP D++ L + A A PAV+ +F+C+
Sbjct: 18 FYILAVLFGAPFFSDIIATAVLATALTAVSALPAVFLFDSEERAVEVILQLFSCEGNPTP 77
Query: 168 WKKYFI----FIVVGGWISCVVIPLDWDRDWQNWPIPIVVGGYLGA 209
+ + F +G W + V PLDWDR WQ +P+P +VG ++GA
Sbjct: 78 KESVLLFNSVFAFLGAWAAAAVHPLDWDRWWQRYPLPSLVGCFIGA 123
>ref|NP_173056.1| (NM_101472) unknown protein [Arabidopsis thaliana]
Length = 98
Score = 100 bits (247), Expect = 3e-20
Identities = 16/73 (21%), Positives = 28/73 (37%)
Query: 41 VYVRKTPLMTFPYHLVALLYYYVFVSSNFNTVKLLSFLIPTQVAYLVLQFNKCTVYGNKI 100
VYV + F +H+V Y +S T++LL + V + F + +
Sbjct: 26 VYVITGLFLIFGFHVVRNKYSVDLISDPTLTLRLLWIIEFPIVVIIYSLFRRNPEKCSYF 85
Query: 101 IKINYSLTIICLG 113
+ SL + G
Sbjct: 86 RAVGRSLVGLIAG 98
>ref|NP_173057.1| (NM_101473) hypothetical protein [Arabidopsis thaliana]
Length = 160
Score = 82.1 bits (201), Expect = 8e-15
Identities = 15/49 (30%), Positives = 25/49 (50%), Gaps = 4/49 (8%)
Query: 155 VYSVFNCDFKVGLWKKYFI----FIVVGGWISCVVIPLDWDRDWQNWPI 199
+ +F +G+ + + ++GGW +PLDW+R WQ WPI
Sbjct: 37 WHRIFASLKPIGIIEHMLLVPAYGAIIGGWFGAWPMPLDWERPWQEWPI 85
CPU time: 61.73 user secs. 1.90 sys. secs 63.63 total secs.
Database: nr
Posted date: Apr 21, 2002 2:19 PM
Number of letters in database: 277,845,442
Number of sequences in database: 887,402
Lambda K H
0.318 0.167 0.504
Gapped
Lambda K H
0.270 0.0586 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 138928183
Number of Sequences: 887402
Number of extensions: 6727991
Number of successful extensions: 32301
Number of sequences better than 10.0: 207
Number of HSP's better than 10.0 without gapping: 27
Number of HSP's successfully gapped in prelim test: 182
Number of HSP's that attempted gapping in prelim test: 32169
Number of HSP's gapped (non-prelim): 253
length of query: 219
length of database: 277,845,442
effective HSP length: 47
effective length of query: 172
effective length of database: 236,137,548
effective search space: 40615658256
effective search space used: 40615658256
T: 11
A: 40
X1: 15 ( 6.9 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (20.9 bits)
S2: 72 (32.1 bits)
