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GPI Anchor Biosynthesis Report: orf6.4571.prot (GAA1 family, Candida albicans)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query=
         (567 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!


Results of PSI-Blast iteration 2

Distribution of 16 Blast Hits on the Query Sequence


Sequences with E-value BETTER than threshold (0.002)

ref|NP_013189.1| (NC_001144) ER protein essential for attaching ... 603 e-171
pir||T32771 hypothetical protein F33D11.9b - Caenorhabditis elegans 585 e-166
gb|AAF46094.1| (AE003435) CG3033 gene product [Drosophila melano... 559 e-158
ref|NP_491700.1| (NM_059299) F33D11.9b.p [Caenorhabditis elegans... 558 e-157
emb|CAB65611.1| (AL136078) putative glycosylphosphatidylinositol... 531 e-150
ref|NP_572273.1| (NM_132045) CG3033 gene product [Drosophila mel... 500 e-140
ref|NP_034461.1| (NM_010331) GPI anchor attachment protein 1 [Mu... 369 e-101
emb|CAB86709.1| (AL163505) hypothetical protein L3640.14 [Leishm... 339 8e-92
ref|NP_197414.1| (NM_121918) GPAA1 - like protein [Arabidopsis t... 258 2e-67
ref|NP_491701.1| (NM_059300) F33D11.9a.p [Caenorhabditis elegans... 186 8e-46
Sequences with E-value WORSE than threshold

sp|P30573|CHS3_CANAL CHITIN SYNTHASE 3 (CHITIN-UDP ACETYL-GLUCOS... 40 0.13
pir||S39951 chitin synthase (EC 2.4.1.16) 3 - yeast (Candida alb... 40 0.14
Alignments
>ref|NP_013189.1| (NC_001144) ER protein essential for attaching GPI
           (glycosylphosphatidylinositol) to protein; Gaa1p
           [Saccharomyces cerevisiae]
 sp|P39012|GAA1_YEAST GAA1 PROTEIN
 pir||S45053 membrane protein END2 - yeast (Saccharomyces cerevisiae)
 emb|CAA55944.1| (X79409) Gaa1 [Saccharomyces cerevisiae]
 gb|AAB67592.1| (U53880) Gaa1p [Saccharomyces cerevisiae]
 emb|CAA97649.1| (Z73260) ORF YLR088w [Saccharomyces cerevisiae]
          Length = 614

 Score =  603 bits (1539), Expect = e-171
 Identities = 226/494 (45%), Positives = 329/494 (65%), Gaps = 21/494 (4%)

Query: 21  MALAETVIRKVKKLGLVPKAIAILPRLSLLVAALSVLWLVTLPQDGNYRNVYISENALMP 80
           MAL E + R++  +GLVP+ IA+LP +S+L A    + +  LP DG YR  YISENALMP
Sbjct: 1   MALLEKLHRRIVDMGLVPRIIALLPVISMLCALFGFISIAILPMDGQYRRTYISENALMP 60

Query: 81  AQANSYFRESEWNIVRGYREEIGKMEEWSVADRNEVIASWLVDSGLQISYHENGFANNTL 140
           +QA SYFRESEWNI+RGYR +I +M   +  +RN ++ SWL + G + + +EN     TL
Sbjct: 61  SQAYSYFRESEWNILRGYRSQIKEMVNMTSMERNNLMGSWLQEFGTKTAIYENEQYGETL 120

Query: 141 YAIMHAPRGENTEAMALVVPWTNSDNEYNEGAMSLAVALARYFTKMSIWSKNIIFVFPET 200
           Y +MHAPRG+ TEAM L VPW NSD+E+N G  +L V+LAR+F++  +WSKNII VF E 
Sbjct: 121 YGVMHAPRGDGTEAMVLAVPWFNSDDEFNIGGAALGVSLARFFSRWPVWSKNIIVVFSEN 180

Query: 201 GHRPLRSWVEAYHTVLDDTAGSIEAAIIMEYGKNGDYFEYYDMFYEGLNGQLPNLDLLNT 260
               LRSWVEAYHT LD T GSIEAA++++Y    D+FEY ++ Y+GLNG+LPNLDL+N 
Sbjct: 181 PRAALRSWVEAYHTSLDLTGGSIEAAVVLDYSSTEDFFEYVEISYDGLNGELPNLDLVNI 240

Query: 261 ANVMTYHEQIPCAMQGMSDRVI---NYSTRLQTLFRGILKLTLVGLTDEVHGCEAFSGWQ 317
           A  +T HE +  ++ G+    +   N+ +RL+ L  GI    L G+  + HG EAFSGW+
Sbjct: 241 AISITEHEGMKVSLHGLPSDQLTNNNFWSRLKILCLGIRDWALSGVK-KPHGNEAFSGWR 299

Query: 318 IQAFTIKVRGTEGKDVTQFGRIVDSTFRSVNNLLEKFHQSFFFYLMLSPKHFVSIGTYLP 377
           IQ+ T+K  G  G D+T FGRI ++ FRS+NNLLEKFHQSFFFYL+L+P+ FVSI +YLP
Sbjct: 300 IQSVTLKAHGNSGHDITTFGRIPEAMFRSINNLLEKFHQSFFFYLLLAPRQFVSISSYLP 359

Query: 378 SAILLAVSYALSSVSAVVVAGFDFRKLYF-----VVVVEIACAILAFVPVNQVMLVAISA 432
           SA+ L++++A+SS++A +   +    L+       ++V     +++FV     +L+  S 
Sbjct: 360 SAVALSIAFAISSLNAFINNAYANISLFSEYNLVALLVWFVSLVISFVVSQAFLLIPSSG 419

Query: 433 VVLLP------------RQAIFSKQAAFSLISIALLAVALLITALLIVHFALAFSIGILA 480
           +++              R+   S+  ++ L ++A L  +L+ T+LL+++FA+A  IG LA
Sbjct: 420 LLMTISMASCFLPLILSRKIHISEPLSYRLKNVAFLYFSLVSTSLLMINFAMALLIGTLA 479

Query: 481 LPLTFVPTLMKNKS 494
            P+TFV T++++ S
Sbjct: 480 FPMTFVKTIVESSS 493
 Score = 99.6 bits (246), Expect = 1e-19
 Identities = 27/86 (31%), Positives = 47/86 (54%), Gaps = 5/86 (5%)

Query: 487 PTLMKNKSRLTAFC-LAVSNPFFVIFVAGKVLGHP----ELFDRLVTAWSDIQCWTWFIV 541
            T  + K +L     L ++NPF  I + G          ++ ++LV+AW D++CW+WF++
Sbjct: 525 ATPQQQKQKLKNLVLLILTNPFISITLFGLFFDDEFHGFDIINKLVSAWLDLKCWSWFVL 584

Query: 542 VLGWFPAWVIITLSYCGYKPVKEKSE 567
            +GW P W++I  S    K V  +S+
Sbjct: 585 CIGWLPCWLLILASSFESKSVVVRSK 610
>pir||T32771 hypothetical protein F33D11.9b - Caenorhabditis elegans
          Length = 885

 Score =  585 bits (1494), Expect = e-166
 Identities = 118/596 (19%), Positives = 220/596 (36%), Gaps = 102/596 (17%)

Query: 26  TVIRKVKKLGLVPKAIAILPRLSLLVAALSVLWLVTLPQDGNYRNVYISENALMPAQANS 85
            ++ K+ K   V   IA +       A LS  WL       N     ISE++LMP     
Sbjct: 293 KILDKILKFRKVALIIAYIG------AYLSAQWLF---DPVNMEVTRISEHSLMPGLVTP 343

Query: 86  YFRESEWNIVRGYR-EEIGKMEEWSVADRNEVIASWLVDSGLQISYHE-------NGFAN 137
            F +S   I    R  ++ K +      + E+I +   D GL+   H+       N    
Sbjct: 344 KFDKSGIAIQLYRRFSDLPKSKS-----QQELIHTIFSDLGLECFTHKWRSKVAGNPMNG 398

Query: 138 NTLYAIMHAPRGENTEAMALVVPWTNSDNEYNEGAMSLAVALARYFTKMSIWSKNIIFVF 197
             +Y  +  PR +  EA  +VV      +E +   MS  +A   Y      W+++I+ VF
Sbjct: 399 ENVYGFIRGPRNDGAEAQMIVV--QLGRSEKSRRMMSRMLAFVDYAKDQVYWARDIVIVF 456

Query: 198 PETGH---------RPLRSWVEAYH---------------TVLDDTAGSIEAAIIMEYGK 233
            + G            L +++  Y                  +    G++   ++ +   
Sbjct: 457 VDGGEKNDSIEKAAFALDAFLLKYQKIEALNSKKSITVEADEIQAQTGALIGGVVYDLSG 516

Query: 234 NGDYFEY-YDMFYEGLNGQLPNLDLLNTANVMT---YHEQIPC--AMQGMSDRVINYSTR 287
                ++  ++   GLNGQ  NLD+ N    +    +H ++     +   S+  + YS  
Sbjct: 517 MAVKGQHIVNIQTNGLNGQQVNLDVFNGITKIADSKHHSRVAIFGTLHRHSNPYMEYSPF 576

Query: 288 LQTLFRGILKLTLVGLTDEVHGCEAFSGWQIQAFTIKVRGTEGKDVTQFGRIVDSTFRSV 347
           +  + R +     + + + +H       + +Q  T+ +  +      Q G+ ++   R +
Sbjct: 577 IVPM-RALYTQAFISI-EGIH--SVLGKYGVQGLTVGL--SHDYSEKQTGQFIEEVSRML 630

Query: 348 NNLLEKFHQSFFFYLMLSPKHFVSIGTYLPSAILLAVSYALSSVSAVVVAGFDFRKL--Y 405
           NN+LE+ HQS+F Y++    HF SI  Y+       +   +     V+ A F++ K+  +
Sbjct: 631 NNVLERLHQSYFMYVLADDLHFSSIAMYI-------IPLTILVSPLVISAYFEWTKIQEF 683

Query: 406 FVVVVEIACAILAFVPVNQVMLVAISAVVLLPRQAIFSKQAAFSLISIALLAVALLITAL 465
              VV +A  +L F        +A + V          +   +  + I         +  
Sbjct: 684 QFPVVHLAFHVLGF-----FYYIATTYVFQTIGTTFVLQWMQWPFVGIDGCDGQFTPSWS 738

Query: 466 LIVHFALAFSIGILALPLTFVPTLMKNKSRLTAFCLAVSNPFFVIFVAGKVLGHPELFDR 525
           LI  F + F I     P  F+ T    +S      LA+ NP  V+      +  P +F +
Sbjct: 739 LINSFLIFFMIP--LGPYVFLKTRSIFRSS----VLAILNPISVLAFVA--INIPGMFGQ 790

Query: 526 LVT-----AWS---------------DIQCWTWFIVVLGWFPAWVIITLSYCGYKP 561
           L       +W                + +   +F++    +P W ++      Y P
Sbjct: 791 LFPTISQDSWLAAAQSIALTAIREDVEFKVNHFFLLCCFGYPIWNMLFACSMRYGP 846
>gb|AAF46094.1| (AE003435) CG3033 gene product [Drosophila melanogaster]
          Length = 674

 Score =  559 bits (1426), Expect = e-158
 Identities = 133/618 (21%), Positives = 247/618 (39%), Gaps = 119/618 (19%)

Query: 48  SLLVAALSVLWLVTLPQDGNYRNVYISENALMPAQANSYFRESEWNIVRGYREEIGKMEE 107
           +L VA ++  + + LP+  +    Y+SENAL P       R     +     EE+ +  +
Sbjct: 29  ALYVAGVAWFFCLALPEFNH--GTYLSENALSPGLVYPEIRIDANRLAIQLLEELQRERK 86

Query: 108 WSVADRNEV-IASWLVDSGLQISYHE----------NGFANNTLYAIMHAPRGENTEAMA 156
             ++      IA+ + + GL+   H             +    +Y I+ APR  +TE + 
Sbjct: 87  DHLSTTPHAWIAAKMNEFGLETHTHNYTLRYPFGGGKEYHGKNIYGILRAPRIASTEGIV 146

Query: 157 LVVPWTNSDNEYNE--GAMSLAVALARYFTKMSIWSKNIIFVFPETGHRPLRSWVEAYHT 214
              P+  + + + +   ++ L +A A +  + + W+K++IF+  E     +++W+EAYH 
Sbjct: 147 FAAPYRAASSVHTDISASVPLLLAFADFARRKNYWAKDLIFLITEQEQLGMQAWLEAYHD 206

Query: 215 ---------------VLDDTAGSIEAAIIMEYGKNGDYFEYYDMFYEGLNGQLPNLDLLN 259
                           L   AGS++AA+ +E        ++ D+  EGLNG+LPNLD+ N
Sbjct: 207 GDRELDLSKAYLRPGNLPARAGSLQAALNIEV--QDLEIDHVDVRIEGLNGKLPNLDMFN 264

Query: 260 TANVMTYHEQIPCAMQGMSDRVINYS-TRLQTLFRGILKLT---LVGLTDEVHGCEAFSG 315
               +   E I    +    +   +S +  +  FR +L +      G+    HG   F  
Sbjct: 265 LVQRIMAREGIASGYKQAPRKKRRHSQSHFEQNFRQMLTMLASQSSGVPTGNHGL--FHR 322

Query: 316 WQIQAFTI---------KVRGTEGKDVTQFGRIVDSTFRSVNNLLEKFHQSFFFYLMLSP 366
           ++I A TI          ++G+ G       + ++   RS+NNLLE+FHQSFFFY+++S 
Sbjct: 323 YRIDALTIAANRRATHATLKGSPGSAAVPLLKAIEGIARSLNNLLERFHQSFFFYVIVSN 382

Query: 367 KHFVSIGTYLPSAILLAVSYALSSVSAV---------VVAGFDFRK-------------- 403
             ++SIG Y+P+ + L     L +             +     + +              
Sbjct: 383 DRYISIGDYMPALVALVACAFLKAYLTWSTLPATNAELEKAAGWLREHEQEAELEENLEP 442

Query: 404 -----LYFVVVVEI-ACAILAF----VPVNQVML-VAISAVVL--------------LPR 438
                 Y  V++ + A  ++ F    +P+ Q  L + + A  L              LP 
Sbjct: 443 DKFELPYGSVLIYLTATLLIGFLCNVLPLQQYFLEIPMGAAPLTTSVLSFLSLIGFVLPF 502

Query: 439 QAIFSKQAAFSLISIALLAVALLITALLIVHFALAFSIGILALPLTFV--------PTLM 490
             +        L    LL     +  + +++FAL     +L +PL            + +
Sbjct: 503 VVVLPPGGLELLHVAFLLIYGCALIVIGLLNFALGLFAAVLTVPLVIALETKEENSRSTL 562

Query: 491 KNKSRLTAFCLAVSNPFFVIFVAGKVLGHPEL-FDRLVTAWSDIQC-------------- 535
           +N  RL    +      +VI +A      PEL   +++   +                  
Sbjct: 563 RNTIRLATLVMNPMMVVYVIVLAMTFYQFPELPVQKIMLRAATAAMDASAYGLIDSVIYG 622

Query: 536 -WTWFIVVLGWFPAWVII 552
            W +F++   + P W+I 
Sbjct: 623 NWLYFVICTIFLPLWIIC 640
>ref|NP_491700.1| (NM_059299) F33D11.9b.p [Caenorhabditis elegans]
 gb|AAK68360.1|AF039720_13 (AF039720) Hypothetical protein F33D11.9b [Caenorhabditis elegans]
          Length = 670

 Score =  558 bits (1423), Expect = e-157
 Identities = 118/643 (18%), Positives = 220/643 (33%), Gaps = 149/643 (23%)

Query: 26  TVIRKVKKLGLVPKAIAILPRLSLLVAALSVLWLVTLPQDGNYRNVYISENALMPAQANS 85
            ++ K+ K   V   IA +       A LS  WL       N     ISE++LMP     
Sbjct: 31  KILDKILKFRKVALIIAYIG------AYLSAQWLF---DPVNMEVTRISEHSLMPGLVTP 81

Query: 86  YFRESEWNIVRGYR-EEIGKMEEWSVADRNEVIASWLVDSGLQISYHE-------NGFAN 137
            F +S   I    R  ++ K +      + E+I +   D GL+   H+       N    
Sbjct: 82  KFDKSGIAIQLYRRFSDLPKSKS-----QQELIHTIFSDLGLECFTHKWRSKVAGNPMNG 136

Query: 138 NTLYAIMHAPRGENTEAMALVVPWTNSDNEYNEGAMSLAVALARYFTKMSIWSKNIIFVF 197
             +Y  +  PR +  EA  +VV      +E +   MS  +A   Y      W+++I+ VF
Sbjct: 137 ENVYGFIRGPRNDGAEAQMIVV--QLGRSEKSRRMMSRMLAFVDYAKDQVYWARDIVIVF 194

Query: 198 PETGH---------RPLRSWVEAYH---------------TVLDDTAGSIEAAIIMEYGK 233
            + G            L +++  Y                  +    G++   ++ +   
Sbjct: 195 VDGGEKNDSIEKAAFALDAFLLKYQKIEALNSKKSITVEADEIQAQTGALIGGVVYDLSG 254

Query: 234 NGDYFEY-YDMFYEGLNGQLPNLDLLNTANVMT---YHEQIPC--AMQGMSDRVINYSTR 287
                ++  ++   GLNGQ  NLD+ N    +    +H ++     +   S+  + YS  
Sbjct: 255 MAVKGQHIVNIQTNGLNGQQVNLDVFNGITKIADSKHHSRVAIFGTLHRHSNPYMEYSPF 314

Query: 288 LQTLFRGILKLTLVGLTDEVHGCEAFSGWQIQAFTIKVRGTEGKDVTQFGRIVDSTFRSV 347
           +  + R +     + + + +H       + +Q  T+ +  +      Q G+ ++   R +
Sbjct: 315 IVPM-RALYTQAFISI-EGIH--SVLGKYGVQGLTVGL--SHDYSEKQTGQFIEEVSRML 368

Query: 348 NNLLEKFHQSFFFYLMLSPKHFVSIGTYLPSAILLAVSYALSSVSAVVVAGFDFRKL--Y 405
           NN+LE+ HQS+F Y++    HF SI  Y+       +   +     V+ A F++ K+  +
Sbjct: 369 NNVLERLHQSYFMYVLADDLHFSSIAMYI-------IPLTILVSPLVISAYFEWTKIQEF 421

Query: 406 FVVVVEIACAILAFVPVNQVMLVAISAVVLLPRQAIFSKQAAFSLISIALLAVALLITAL 465
              VV +A  +L F        +A + V          +   +  + I         +  
Sbjct: 422 QFPVVHLAFHVLGF-----FYYIATTYVFQTIGTTFVLQWMQWPFVGIDGCDGQFTPSWS 476

Query: 466 LIVHFALAFSIGILALPLTFVP-------------------------------------- 487
           LI  F + F I     P  F+                                       
Sbjct: 477 LINSFLIFFMIP--LGPYVFLKEPVRCVPSMRIVCLLETSLVLYGICLTNFGLAILCTAF 534

Query: 488 ---------TLMKNKSRLTAFCLAVSNPFFVIFVAGKVLGHPELFDRLVT-----AWS-- 531
                    T    +S      LA+ NP  V+      +  P +F +L       +W   
Sbjct: 535 VVPIVFLMTTRSIFRSS----VLAILNPISVLAFVA--INIPGMFGQLFPTISQDSWLAA 588

Query: 532 -------------DIQCWTWFIVVLGWFPAWVIITLSYCGYKP 561
                        + +   +F++    +P W ++      Y P
Sbjct: 589 AQSIALTAIREDVEFKVNHFFLLCCFGYPIWNMLFACSMRYGP 631
>emb|CAB65611.1| (AL136078) putative glycosylphosphatidylinositol anchor attachment
           protein [Schizosaccharomyces pombe]
          Length = 581

 Score =  531 bits (1356), Expect = e-150
 Identities = 152/551 (27%), Positives = 262/551 (46%), Gaps = 55/551 (9%)

Query: 32  KKLGLVPKAIAILPRLSLLVAALSVLWLVTLPQDGNYRNVYISENALMPAQANSYFRESE 91
            ++ + P     L  L L +  + + W+  LP++     +++SE+AL+P Q N+YF   E
Sbjct: 7   VQIRVFPFLQRHLFFLQLSLTLIGLSWIFILPRNEIIDRLHVSESALLPGQVNTYF---E 63

Query: 92  WNIVRGYREEIGKMEEWSVADRNEVIASWLVDSGLQIS-----------YHEN----GFA 136
               +     +     WS  D + V  + + D   QI            Y  N     F 
Sbjct: 64  NRYSKTVSSSLTAANTWSHLDAS-VGTNTMYDDLEQIFTAMGLPTQKQNYSINIPGSEFN 122

Query: 137 NNTLYAIMHAPRGENTEAMALVVPWTNSDNEYNEGAMSLAVALARYFTKMSIWSKNIIFV 196
            +     + APRG+ TE++ L VPW +   +YNE  ++LA++L +YF   S+WSK+II V
Sbjct: 123 GSNFITTLRAPRGDATESLLLCVPWKDHIGQYNEAGVALAISLLKYFQGWSLWSKDIILV 182

Query: 197 FPETGHRPLRSWVEAY---------HTVLDDTAGSIEAAIIMEYGKNGDYFEYYDMFYEG 247
             +       S++ +Y         +T L   +GSI+A + +E     +  +  ++ Y+ 
Sbjct: 183 IFDDPVYGPSSFLTSYFDQTTPYISYTPLKIRSGSIQAGLSLELVTTENNSDVLEVLYQA 242

Query: 248 LNGQLPNLDLLNTA-NVMTYHEQIPCAMQG---MSDRVINYSTRLQTLFRGILKLTLVGL 303
            NGQLPNLDL NT   +   H   P  +QG    ++   +Y++RL++L+ G+L   +  +
Sbjct: 243 TNGQLPNLDLFNTISRIFMQHFNYPLRLQGYDFHANSGSSYTSRLKSLWMGMLTQAVSNV 302

Query: 304 TDEVHGCEAFSGWQIQAFTI--KVRGTEGKDVTQFGRIVDSTFRSVNNLLEKFHQSFFFY 361
               H    F  ++I   T+  KV+     D+ +FG+ ++STFRS+NNLLE  HQSFFFY
Sbjct: 303 -TSAHAL--FPQYRIDMLTLRMKVKDPFSFDMFRFGQAIESTFRSLNNLLEHLHQSFFFY 359

Query: 362 LMLSPKHFVSIGTYLPSAILLAVSYALSSVSAVVV--AGFDFRKLYFVVVVEIACAILAF 419
            +L   HF+SIG Y+PS ++LA S+ L +    +      D  + +   +  I C I A+
Sbjct: 360 FILDHLHFISIGNYMPSILILAASFMLGAYRHWINHEKKIDLWRPFSFWLFSIFCTIAAY 419

Query: 420 VPVNQ-------VMLVAISAVVLLPRQAIFSKQAAFSLISIALLAVALLITALLIVHFAL 472
             V+        + L  +   + +      + + A  ++S  L++ +L I+ +  ++F+L
Sbjct: 420 YLVSSSTKITVFIFLYLMLTFIGIIFSTFMTSEDAELVLSYDLMSKSLFISVVSTLNFSL 479

Query: 473 AFSIGILALPLTFVPTLMKNKSRLTAFCLAVSNPFFVIFVAGKVLGHPELFDRLVTAWSD 532
           +F + IL +PL F+      +  L    L   + F  +    K+L  P     LV  W  
Sbjct: 480 SFVVAILLVPLQFISFRFNRRLSLLFAVLTYFSTFIFLCSLSKILNGP-----LVPFWLW 534

Query: 533 IQ----CWTWF 539
            +      +W 
Sbjct: 535 AKEYELFNSWL 545
>ref|NP_572273.1| (NM_132045) CG3033 gene product [Drosophila melanogaster]
 gb|AAK77275.1| (AY047543) GH05723p [Drosophila melanogaster]
          Length = 561

 Score =  500 bits (1276), Expect = e-140
 Identities = 119/528 (22%), Positives = 218/528 (40%), Gaps = 95/528 (17%)

Query: 48  SLLVAALSVLWLVTLPQDGNYRNVYISENALMPAQANSYFRESEWNIVRGYREEIGKMEE 107
           +L VA ++  + + LP+  +    Y+SENAL P       R     +     EE+ +  +
Sbjct: 29  ALYVAGVAWFFCLALPEFNH--GTYLSENALSPGLVYPEIRIDANRLAIQLLEELQRERK 86

Query: 108 WSVADRNEV-IASWLVDSGLQISYHE----------NGFANNTLYAIMHAPRGENTEAMA 156
             ++      IA+ + + GL+   H             +    +Y I+ APR  +TE + 
Sbjct: 87  DHLSTTPHAWIAAKMNEFGLETHTHNYTLRYPFGGGKEYHGKNIYGILRAPRIASTEGIV 146

Query: 157 LVVPWTNSDNEYNE--GAMSLAVALARYFTKMSIWSKNIIFVFPETGHRPLRSWVEAYHT 214
              P+  + + + +   ++ L +A A +  + + W+K++IF+  E     +++W+EAYH 
Sbjct: 147 FAAPYRAASSVHTDISASVPLLLAFADFARRKNYWAKDLIFLITEQEQLGMQAWLEAYHD 206

Query: 215 ---------------VLDDTAGSIEAAIIMEYGKNGDYFEYYDMFYEGLNGQLPNLDLLN 259
                           L   AGS++AA+ +E        ++ D+  EGLNG+LPNLD+ N
Sbjct: 207 GDRELDLSKAYLRPGNLPARAGSLQAALNIEV--QDLEIDHVDVRIEGLNGKLPNLDMFN 264

Query: 260 TANVMTYHEQIPCAMQGMSDRVINYS-TRLQTLFRGILKLT---LVGLTDEVHGCEAFSG 315
               +   E I    +    +   +S +  +  FR +L +      G+    HG   F  
Sbjct: 265 LVQRIMAREGIASGYKQAPRKKRRHSQSHFEQNFRQMLTMLASQSSGVPTGNHGL--FHR 322

Query: 316 WQIQAFTI---------KVRGTEGKDVTQFGRIVDSTFRSVNNLLEKFHQSFFFYLMLSP 366
           ++I A TI          ++G+ G       + ++   RS+NNLLE+FHQSFFFY+++S 
Sbjct: 323 YRIDALTIAANRRATHATLKGSPGSAAVPLLKAIEGIARSLNNLLERFHQSFFFYVIVSN 382

Query: 367 KHFVSIGTYLPSAILLAVSYALSSVSAV---------VVAGFDFRK-------------- 403
             ++SIG Y+P+ + L     L +             +     + +              
Sbjct: 383 DRYISIGDYMPALVALVACAFLKAYLTWSTLPATNAELEKAAGWLREHEQEAELEENLEP 442

Query: 404 -----LYFVVVVEI-ACAILAF----VPVNQVML-VAISAVVL--------------LPR 438
                 Y  V++ + A  ++ F    +P+ Q  L + + A  L              LP 
Sbjct: 443 DKFELPYGSVLIYLTATLLIGFLCNVLPLQQYFLEIPMGAAPLTTSVLSFLSLIGFVLPF 502

Query: 439 QAIFSKQAAFSLISIALLAVALLITALLIVHFALAFSIGILALPLTFV 486
             +        L    LL     +  + +++FAL     +L +PL   
Sbjct: 503 VVVLPPGGLELLHVAFLLIYGCALIVIGLLNFALGLFAAVLTVPLVIA 550
>ref|NP_034461.1| (NM_010331) GPI anchor attachment protein 1 [Mus musculus]
 dbj|BAA82589.1| (AB002136) glycosylphosphatidylinositol anchor attachment 1 (GPAA1)
           [Mus musculus]
 dbj|BAA82591.1| (AB002138) glycosylphosphatidylinositol anchor attachment 1 (GPAA1)
           [Mus musculus]
 dbj|BAB03274.1| (AB006970) GPI anchor attachment protein [Mus musculus]
 dbj|BAB03275.1| (AB006971) GPI anchor attachment protein [Mus musculus]
 gb|AAH06697.1|AAH06697 (BC006697) GPI anchor attachment protein 1 [Mus musculus]
          Length = 621

 Score =  369 bits (940), Expect = e-101
 Identities = 105/384 (27%), Positives = 167/384 (43%), Gaps = 44/384 (11%)

Query: 29  RKVKKLGLVPKAIAILPRLSLLVAALSVLWLVTLPQDGNYRNVYISENALMPAQANSYFR 88
             V++  L    + +   L +L     + W + L      +  Y+SENA+        F 
Sbjct: 6   DPVRRRALARIVLRLNTPLCVLSYVAGIAWFLALAFPPLTQRTYMSENAMGSTMVEEQFV 65

Query: 89  ESEWNIVRGYREEIGKMEEWSVADRNEVIASWLVDS----GLQI-------------SYH 131
             +            + +  ++        +WL  S    GL++               H
Sbjct: 66  GGDRARSFARDFAAHRKKPGALP------VAWLERSMRSVGLEVYTQSFSRKLPFPDETH 119

Query: 132 ENGFA-NNTLYAIMHAPRGENTEAMALVVPWTNSDNEYNEGAMSLAVALARYFTKMSIWS 190
           E        +Y I+ APR  +TE++ L VP     +  N  A+ L +ALA +F     W+
Sbjct: 120 ERYMVSGTNVYGILRAPRSASTESLVLTVPC--GPDATNSQAVGLLLALAAHFRGQIYWA 177

Query: 191 KNIIFVFPETGHRPLRSWVEAYHT---------VLDDTAGSIEAAIIMEYGKNGDYFEYY 241
           K+IIF+  +       +W+EAYH           L   AG+I+AA+ +E     D     
Sbjct: 178 KDIIFLVTDHDLLGTEAWLEAYHDINVTGIQSSPLQGRAGAIQAAVALELS--SDVVTSL 235

Query: 242 DMFYEGLNGQLPNLDLLNTANVMTYHEQIPCAMQG--MSDRVINYSTRLQTLFRGILKLT 299
           D+  EGLNGQLPNLDLLN          + C +QG        +    LQ L + +L + 
Sbjct: 236 DVTVEGLNGQLPNLDLLNLFQTFCQKGGLLCTLQGKLQPQDWTSLEGPLQGL-QTLLLMV 294

Query: 300 LVGLTDEVHGCEA-FSGWQIQAFTIKVRGT---EGKDVTQFGRIVDSTFRSVNNLLEKFH 355
           L   +   HG    F  + ++A T++   +      D+   G+ ++  FR +N+LLE+ H
Sbjct: 295 LRQASGRPHGPHGLFLRYGVEALTLRGINSFRQYKYDLATVGKALEGMFRKLNHLLERLH 354

Query: 356 QSFFFYLMLSPKHFVSIGTYLPSA 379
           QSFFFYL+ +   FVSIG Y+P+ 
Sbjct: 355 QSFFFYLLPALSRFVSIGLYMPAT 378
 Score = 42.7 bits (100), Expect = 0.016
 Identities = 34/192 (17%), Positives = 69/192 (35%), Gaps = 28/192 (14%)

Query: 386 YALSSVSAVVVAGFDFRKLYFVVVVEIACAILAFVPVNQVMLVAISAVV-----LLPRQA 440
            A  +   ++        LYF+ V+    A   F       +V     +      LP   
Sbjct: 424 LASLTAPLLISQAMG-LALYFLPVLGQHLATQHFPVAEAEAVVLTLLAIYVAGLALPHNT 482

Query: 441 I------FSKQAAFSLISIALLAVALLITALLIVHFALAFSIGILALPLTFVPTLMKNKS 494
                     +   +L  +AL+ +AL +  + +++F+L F +    +P   +      ++
Sbjct: 483 HRVVNSQVPDRGWMALKLVALIYLALQLGCIALLNFSLGFLLAATMVPAAALAKPHGPRT 542

Query: 495 RLTAFCLAVSNP----FFVIFVAGKVLGHPEL---FDRLVTAWS--------DIQCWTWF 539
            L A  L V++P    F  +F+  ++L  P       +L                   + 
Sbjct: 543 -LYAALLVVTSPAVTLFGSLFLWRELLEVPLSLAEGWQLFLTALAQGVLEHYTYGALLFP 601

Query: 540 IVVLGWFPAWVI 551
           I+ LG +P W++
Sbjct: 602 ILALGLYPCWLL 613
>emb|CAB86709.1| (AL163505) hypothetical protein L3640.14 [Leishmania major]
          Length = 464

 Score =  339 bits (861), Expect = 8e-92
 Identities = 68/358 (18%), Positives = 115/358 (31%), Gaps = 33/358 (9%)

Query: 145 HAPRGENTEAMALVVPWTNSDNEYNEGAMSLAVALARYFTKMSIWSKNIIFVFPETGHRP 204
              R   +E   +   + N+D      ++SLA AL     +    + ++ F F  +    
Sbjct: 75  RGRRSPGSE---IAAVYVNTD---YPASVSLANALIEVIRRRKSLACDVQFYFVNSS--- 125

Query: 205 LRSWVEAYHTVLDDTAGSIEAAIIMEYGKNGDYFEYYDMFYEGLNGQLPNLDLLNTANVM 264
              W                AA+++       Y  +  +   G NG  PN D  N A  +
Sbjct: 126 -EEW--------PVPQAYTRAALVLNLST--LYNRHICIDTLGGNGIQPNQDYTNIAVQL 174

Query: 265 TYHEQIPCAMQGMSDRVINYSTRLQTLFRGILKLTLVGLTDEVHGCEAFSGWQIQAFTIK 324
               Q   +                           V L              I    I 
Sbjct: 175 AAANQFLPSYLCQRRHRSTDDAHAGFWHYWAYLEASVQLPTRPQPWHIIPTHSINTIAIS 234

Query: 325 VRGTEGKDVT--------QFGRIVDSTFRSVNNLLEKFHQSFFFYLMLSPKHFVSIGTYL 376
                              F ++V     S+N L EKFH S F +L +S   +V      
Sbjct: 235 SDPLYSASSVFPHSQLPDAFAQLVLKIIVSLNGLEEKFHHSTFVWLPVSLWRYVEYDHAQ 294

Query: 377 PSAILLAVSYALSSVSAVVVAGFDFRKLYFVVVVEIACAILAFVPVNQVMLVAISAVVLL 436
              IL   S   ++ S   + G     L+  +++    A  AF       +V+ SA   L
Sbjct: 295 FCIILFVASIFSTTYSEYQLRGISITPLFVTLLLTPVAAAAAFQVAGWWAVVSASAAFSL 354

Query: 437 PRQAIFSKQAAFSLISIALLAVALLITALLIVHFALAFSIG-ILALPLTFVPTLMKNK 493
             + +     +  L+S   +A+ LLI    I+  A     G   A+ ++F+ T ++N+
Sbjct: 355 LFRLLMGHANSGMLLSFNAIALCLLI----ILQPACGLVAGAAAAIQVSFIHTALQNR 408
>ref|NP_197414.1| (NM_121918) GPAA1 - like protein [Arabidopsis thaliana]
          Length = 696

 Score =  258 bits (654), Expect = 2e-67
 Identities = 74/331 (22%), Positives = 117/331 (34%), Gaps = 71/331 (21%)

Query: 290 TLFRGILKLTLVGLTDEVHGCEAFSGWQIQAFTIKVRG-------TEGKDVTQFG-RIVD 341
           TL   +    L G+    HG  AF  +Q+ A T+KV         T   D    G R+++
Sbjct: 363 TLASSLYSQAL-GIPTGPHG--AFRDYQVDAITLKVSPRFPPDSKTRQHDFFLRGARLLE 419

Query: 342 STFRSVNNLLEKFHQSFFFYLMLSPKHFVSIGTYLPSAILLAVSYALSSVSAVVV----- 396
            T RSVNNLLEKFHQSFF Y++ SP  F+S+G Y+ +  LL     + + S  +      
Sbjct: 420 GTIRSVNNLLEKFHQSFFLYMLTSPSKFISVGVYMIAFALLVAPLPMVAASLYIDGCKSL 479

Query: 397 --------AGFDFRKLYFVVVVEIACAILAFVP--------------------------V 422
                     F   K            +L F+                            
Sbjct: 480 TNSTHNPTENFKSWKWLDAAKQVFTLHLLGFIVTLLPYFICQVPGQHSPTNRSIMWGTTS 539

Query: 423 NQVMLVAISAVVLL-PRQAIFSKQAAFSLISIALLAVALLITALLIVHFALAFSIGILAL 481
           + ++L+    +    P  +         L S+ + A  + +  + I++FA A    +L +
Sbjct: 540 SSLLLITFVTMPGCSPFSSRLHGTNWAILKSVTISAAFIGLCLMSIINFATAEIGALLLV 599

Query: 482 PLTFVPTLMKNKSR-------LTAFCLAVSNPFFVIFVAGKVLGHPELFDRLVTAWSDIQ 534
           P   +   +K   R       L AFC  V        +   +     L + LV      +
Sbjct: 600 PTCLMAQPIKPALRSRRLKSLLGAFCSIVLLTIGFPVMFFAI-SKGLLGEGLVGLSLGGE 658

Query: 535 CWTWFIVVLGW------------FPAWVIIT 553
            WTW   +  W             P W++  
Sbjct: 659 FWTWLESLWAWKSATYLYIGMVHLPCWLLCL 689
 Score =  245 bits (620), Expect = 2e-63
 Identities = 71/290 (24%), Positives = 128/290 (43%), Gaps = 53/290 (18%)

Query: 29  RKVKKLGLVPKAIAILPRLSLLVAALSVLWLVTLPQDGNYRNVYISENALMPAQANSYFR 88
           R + +LG+    IA  P  S++ +A  V  L  L      +N YISENALMP  A S   
Sbjct: 24  RPIVRLGIF--LIAHSPVFSVVFSAAGV--LALLLLPLLAKNTYISENALMPGSARSMLS 79

Query: 89  ESEWNIVRGYREEIGKMEEWSVADRNEVIASWLVDSGLQISY---HENGFANNTLY---- 141
             + +      ++I            ++I  ++ D G ++SY   H  G   + L+    
Sbjct: 80  NRDVSDGSKLVKDIKNFRLNHEGQVQKLIGKYMSDMGAEVSYQKFHPEGNQFHPLHFFSG 139

Query: 142 --------------------AIMHAPRGENTEAMALVVPWTN-SDNEYNEGAMSLAVALA 180
                                I+ APRG+  E++ LV P+   +  +Y + ++ +  +L 
Sbjct: 140 PDSYTLLENVSCASYGVNVAGIIRAPRGDGKESIVLVTPYDFINGGDYEDLSLGIVSSLF 199

Query: 181 RYFTKMSIWSKNIIFVFPET---GHRPLRSWVEAYHTVL-----------------DDTA 220
              ++++  SK+II++  ++    +RP+ +W+  YH+                      A
Sbjct: 200 SLLSRVTWLSKDIIWLVADSRYGDYRPVAAWLTEYHSPSFKVSDLLKCDEQNTADNFRRA 259

Query: 221 GSIEAAIIMEYGKNGDYFE-YYDMFYEGLNGQLPNLDLLNTANVMTYHEQ 269
           G++ AA++++     + FE    ++ E  NGQ+PNLDL+N  N +  H Q
Sbjct: 260 GTMAAALVLKVDGRSEKFEDTLSIYAEASNGQMPNLDLINVVNYLAVHRQ 309
>ref|NP_491701.1| (NM_059300) F33D11.9a.p [Caenorhabditis elegans]
 pir||T32772 hypothetical protein F33D11.9a - Caenorhabditis elegans
 gb|AAB96702.1| (AF039720) Hypothetical protein F33D11.9a [Caenorhabditis elegans]
          Length = 229

 Score =  186 bits (469), Expect = 8e-46
 Identities = 35/200 (17%), Positives = 67/200 (33%), Gaps = 35/200 (17%)

Query: 384 VSYALSSVSAVVVAGFDFRKL--YFVVVVEIACAILAFVPVNQVMLVAISAVVLLPRQAI 441
           +   +     V+ A F++ K+  +   VV +A  +L F        +A + V        
Sbjct: 4   IPLTILVSPLVISAYFEWTKIQEFQFPVVHLAFHVLGF-----FYYIATTYVFQTIGTTF 58

Query: 442 FSKQAAFSLISIALLAVALLITALLIVHFALAFSIGILALPLTFVPTLMKNKSRLTAFCL 501
             +   +  + I         +  LI  F + F I     P  F+ T    +S      L
Sbjct: 59  VLQWMQWPFVGIDGCDGQFTPSWSLINSFLIFFMIP--LGPYVFLKTRSIFRSS----VL 112

Query: 502 AVSNPFFVIFVAGKVLGHPELFDRLVT-----AWS---------------DIQCWTWFIV 541
           A+ NP  V+      +  P +F +L       +W                + +   +F++
Sbjct: 113 AILNPISVLAFVA--INIPGMFGQLFPTISQDSWLAAAQSIALTAIREDVEFKVNHFFLL 170

Query: 542 VLGWFPAWVIITLSYCGYKP 561
               +P W ++      Y P
Sbjct: 171 CCFGYPIWNMLFACSMRYGP 190
 Score =  184 bits (464), Expect = 3e-45
 Identities = 36/210 (17%), Positives = 69/210 (32%), Gaps = 42/210 (20%)

Query: 374 TYLPSAILLAVSYALSSVSAVVVAGFDFRKL--YFVVVVEIACAILAFVPVNQVMLVAIS 431
            Y+       +   +     V+ A F++ K+  +   VV +A  +L F        +A +
Sbjct: 1   MYI-------IPLTILVSPLVISAYFEWTKIQEFQFPVVHLAFHVLGF-----FYYIATT 48

Query: 432 AVVLLPRQAIFSKQAAFSLISIALLAVALLITALLIVHFALAFSIGILALPLTFVPTLMK 491
            V          +   +  + I         +  LI  F + F I     P  F+ T   
Sbjct: 49  YVFQTIGTTFVLQWMQWPFVGIDGCDGQFTPSWSLINSFLIFFMIP--LGPYVFLKTRSI 106

Query: 492 NKSRLTAFCLAVSNPFFVIFVAGKVLGHPELFDRLVT-----AWS--------------- 531
            +S      LA+ NP  V+      +  P +F +L       +W                
Sbjct: 107 FRSS----VLAILNPISVLAFVA--INIPGMFGQLFPTISQDSWLAAAQSIALTAIREDV 160

Query: 532 DIQCWTWFIVVLGWFPAWVIITLSYCGYKP 561
           + +   +F++    +P W ++      Y P
Sbjct: 161 EFKVNHFFLLCCFGYPIWNMLFACSMRYGP 190
>sp|P30573|CHS3_CANAL CHITIN SYNTHASE 3 (CHITIN-UDP ACETYL-GLUCOSAMINYL TRANSFERASE 3)
            (CLASS-IV CHITIN SYNTHASE 3)
 dbj|BAA02707.1| (D13454) chitin synthase III [Candida albicans]
          Length = 1213

 Score = 39.6 bits (92), Expect = 0.13
 Identities = 18/122 (14%), Positives = 43/122 (34%), Gaps = 6/122 (4%)

Query: 438  RQAIFSKQAAFSLISI-ALLAVALLITALLIVHFALAFSIGILALPLTFVPTLMKNKSR- 495
             + + S++  +   ++  L  + L+        F++ F I I  +    +P  +      
Sbjct: 996  FKVLLSQRRRWINSTVHNLFELVLVKDLCGTFCFSMQFVIFIELIGTLVLPAAITFTIYV 1055

Query: 496  -LTAFCLAVSNPFFVIFVAGKVLGHPELFDRLVTAWSDIQCWTWFIVVLGWFPAWVIITL 554
             + A     + P   + +   + G P     +  +        +F++ L   P W  +  
Sbjct: 1056 IIVAIVSKPT-PVMSLVLLAVIFGLPGCLIVITVSSLSYLV--YFVIYLFALPIWNFVLP 1112

Query: 555  SY 556
            SY
Sbjct: 1113 SY 1114
>pir||S39951 chitin synthase (EC 2.4.1.16) 3 - yeast (Candida albicans)
 prf||2009366A chitin synthase 3 [Candida albicans]
          Length = 1175

 Score = 39.6 bits (92), Expect = 0.14
 Identities = 18/122 (14%), Positives = 43/122 (34%), Gaps = 6/122 (4%)

Query: 438  RQAIFSKQAAFSLISI-ALLAVALLITALLIVHFALAFSIGILALPLTFVPTLMKNKSR- 495
             + + S++  +   ++  L  + L+        F++ F I I  +    +P  +      
Sbjct: 958  FKVLLSQRRRWINSTVHNLFELVLVKDLCGTFCFSMQFVIFIELIGTLVLPAAITFTIYV 1017

Query: 496  -LTAFCLAVSNPFFVIFVAGKVLGHPELFDRLVTAWSDIQCWTWFIVVLGWFPAWVIITL 554
             + A     + P   + +   + G P     +  +        +F++ L   P W  +  
Sbjct: 1018 IIVAIVSKPT-PVMSLVLLAVIFGLPGCLIVITVSSLSYLV--YFVIYLFALPIWNFVLP 1074

Query: 555  SY 556
            SY
Sbjct: 1075 SY 1076
CPU time:   145.30 user secs.	    2.97 sys. secs	  148.27 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.316    0.177    0.485 

Gapped
Lambda     K      H
   0.270   0.0620    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 366532228
Number of Sequences: 887402
Number of extensions: 18168404
Number of successful extensions: 76332
Number of sequences better than 10.0: 417
Number of HSP's better than 10.0 without gapping: 33
Number of HSP's successfully gapped in prelim test: 398
Number of HSP's that attempted gapping in prelim test: 75917
Number of HSP's gapped (non-prelim): 664
length of query: 567
length of database: 277,845,442
effective HSP length: 56
effective length of query: 511
effective length of database: 228,150,930
effective search space: 116585125230
effective search space used: 116585125230
T: 11
A: 40
X1: 15 ( 6.8 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (20.7 bits)
S2: 76 (33.6 bits)