IMP GPI Biosynthesis Report IMP-Bioinformatics
GPI Biosynthesis Main Page  
GPI Site Motif   GPI Site Prediction   Home Page B.E.  

GPI Anchor Biosynthesis Report: NP_034461.1 (GAA1 family, Mus musculus)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query=
         (621 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!


Results of PSI-Blast iteration 3

Distribution of 14 Blast Hits on the Query Sequence


Sequences with E-value BETTER than threshold (0.002)

ref|NP_034461.1| (NM_010331) GPI anchor attachment protein 1 [Mu... 871 0.0
ref|NP_003792.1| (NM_003801) anchor attachment protein 1; anchor... 844 0.0
gb|AAF46094.1| (AE003435) CG3033 gene product [Drosophila melano... 727 0.0
ref|NP_491700.1| (NM_059299) F33D11.9b.p [Caenorhabditis elegans... 714 0.0
pir||T46923 hypothetical protein DKFZp434E1512.1 - human >gi|701... 682 0.0
ref|NP_572273.1| (NM_132045) CG3033 gene product [Drosophila mel... 648 0.0
ref|NP_197414.1| (NM_121918) GPAA1 - like protein [Arabidopsis t... 634 e-180
pir||T32771 hypothetical protein F33D11.9b - Caenorhabditis elegans 587 e-166
ref|NP_013189.1| (NC_001144) ER protein essential for attaching ... 533 e-150
emb|CAB86709.1| (AL163505) hypothetical protein L3640.14 [Leishm... 274 4e-72
ref|NP_491701.1| (NM_059300) F33D11.9a.p [Caenorhabditis elegans... 132 1e-29
Sequences with E-value WORSE than threshold

ref|NP_187166.1| (NM_111387) putative sugar transporter [Arabido... 36 1.6
Alignments
>ref|NP_034461.1| (NM_010331) GPI anchor attachment protein 1 [Mus musculus]
 dbj|BAA82589.1| (AB002136) glycosylphosphatidylinositol anchor attachment 1 (GPAA1)
           [Mus musculus]
 dbj|BAA82591.1| (AB002138) glycosylphosphatidylinositol anchor attachment 1 (GPAA1)
           [Mus musculus]
 dbj|BAB03274.1| (AB006970) GPI anchor attachment protein [Mus musculus]
 dbj|BAB03275.1| (AB006971) GPI anchor attachment protein [Mus musculus]
 gb|AAH06697.1|AAH06697 (BC006697) GPI anchor attachment protein 1 [Mus musculus]
          Length = 621

 Score =  871 bits (2227), Expect = 0.0
 Identities = 621/621 (100%), Positives = 621/621 (100%)

Query: 1   MGLLSDPVRRRALARIVLRLNTPLCVLSYVAGIAWFLALAFPPLTQRTYMSENAMGSTMV 60
           MGLLSDPVRRRALARIVLRLNTPLCVLSYVAGIAWFLALAFPPLTQRTYMSENAMGSTMV
Sbjct: 1   MGLLSDPVRRRALARIVLRLNTPLCVLSYVAGIAWFLALAFPPLTQRTYMSENAMGSTMV 60

Query: 61  EEQFVGGDRARSFARDFAAHRKKPGALPVAWLERSMRSVGLEVYTQSFSRKLPFPDETHE 120
           EEQFVGGDRARSFARDFAAHRKKPGALPVAWLERSMRSVGLEVYTQSFSRKLPFPDETHE
Sbjct: 61  EEQFVGGDRARSFARDFAAHRKKPGALPVAWLERSMRSVGLEVYTQSFSRKLPFPDETHE 120

Query: 121 RYMVSGTNVYGILRAPRSASTESLVLTVPCGPDATNSQAVGLLLALAAHFRGQIYWAKDI 180
           RYMVSGTNVYGILRAPRSASTESLVLTVPCGPDATNSQAVGLLLALAAHFRGQIYWAKDI
Sbjct: 121 RYMVSGTNVYGILRAPRSASTESLVLTVPCGPDATNSQAVGLLLALAAHFRGQIYWAKDI 180

Query: 181 IFLVTDHDLLGTEAWLEAYHDINVTGIQSSPLQGRAGAIQAAVALELSSDVVTSLDVTVE 240
           IFLVTDHDLLGTEAWLEAYHDINVTGIQSSPLQGRAGAIQAAVALELSSDVVTSLDVTVE
Sbjct: 181 IFLVTDHDLLGTEAWLEAYHDINVTGIQSSPLQGRAGAIQAAVALELSSDVVTSLDVTVE 240

Query: 241 GLNGQLPNLDLLNLFQTFCQKGGLLCTLQGKLQPQDWTSLEGPLQGLQTLLLMVLRQASG 300
           GLNGQLPNLDLLNLFQTFCQKGGLLCTLQGKLQPQDWTSLEGPLQGLQTLLLMVLRQASG
Sbjct: 241 GLNGQLPNLDLLNLFQTFCQKGGLLCTLQGKLQPQDWTSLEGPLQGLQTLLLMVLRQASG 300

Query: 301 RPHGPHGLFLRYGVEALTLRGINSFRQYKYDLATVGKALEGMFRKLNHLLERLHQSFFFY 360
           RPHGPHGLFLRYGVEALTLRGINSFRQYKYDLATVGKALEGMFRKLNHLLERLHQSFFFY
Sbjct: 301 RPHGPHGLFLRYGVEALTLRGINSFRQYKYDLATVGKALEGMFRKLNHLLERLHQSFFFY 360

Query: 361 LLPALSRFVSIGLYMPATGFLLLVLGLKALELWMQLHQAGVNPEEAGKAPSPGTPLLPTQ 420
           LLPALSRFVSIGLYMPATGFLLLVLGLKALELWMQLHQAGVNPEEAGKAPSPGTPLLPTQ
Sbjct: 361 LLPALSRFVSIGLYMPATGFLLLVLGLKALELWMQLHQAGVNPEEAGKAPSPGTPLLPTQ 420

Query: 421 GVGLASLTAPLLISQAMGLALYFLPVLGQHLATQHFPVAEAEAVVLTLLAIYVAGLALPH 480
           GVGLASLTAPLLISQAMGLALYFLPVLGQHLATQHFPVAEAEAVVLTLLAIYVAGLALPH
Sbjct: 421 GVGLASLTAPLLISQAMGLALYFLPVLGQHLATQHFPVAEAEAVVLTLLAIYVAGLALPH 480

Query: 481 NTHRVVNSQVPDRGWMALKLVALIYLALQLGCIALLNFSLGFLLAATMVPAAALAKPHGP 540
           NTHRVVNSQVPDRGWMALKLVALIYLALQLGCIALLNFSLGFLLAATMVPAAALAKPHGP
Sbjct: 481 NTHRVVNSQVPDRGWMALKLVALIYLALQLGCIALLNFSLGFLLAATMVPAAALAKPHGP 540

Query: 541 RTLYAALLVVTSPAVTLFGSLFLWRELLEVPLSLAEGWQLFLTALAQGVLEHYTYGALLF 600
           RTLYAALLVVTSPAVTLFGSLFLWRELLEVPLSLAEGWQLFLTALAQGVLEHYTYGALLF
Sbjct: 541 RTLYAALLVVTSPAVTLFGSLFLWRELLEVPLSLAEGWQLFLTALAQGVLEHYTYGALLF 600

Query: 601 PILALGLYPCWLLFWNVLFWK 621
           PILALGLYPCWLLFWNVLFWK
Sbjct: 601 PILALGLYPCWLLFWNVLFWK 621
>ref|NP_003792.1| (NM_003801) anchor attachment protein 1; anchor attachment protein
           1 (Gaa1p, yeast) homolog; glycophosphatidylinositol
           anchor attachment 1 [Homo sapiens]
 dbj|BAA24035.1| (AB006969) hGAA1 [Homo sapiens]
 dbj|BAA82588.1| (AB002135) glycosylphosphatidylinositol anchor attachment 1 (GPAA1)
           [Homo sapiens]
 dbj|BAA82590.1| (AB002137) glycosylphosphatidylinositol anchor attachment 1 (GPAA1)
           [Homo sapiens]
 gb|AAH03171.1|AAH03171 (BC003171) anchor attachment protein 1 (Gaa1p, yeast) homolog [Homo
           sapiens]
 gb|AAH04129.1|AAH04129 (BC004129) anchor attachment protein 1 (Gaa1p, yeast) homolog [Homo
           sapiens]
          Length = 621

 Score =  844 bits (2158), Expect = 0.0
 Identities = 567/621 (91%), Positives = 588/621 (94%)

Query: 1   MGLLSDPVRRRALARIVLRLNTPLCVLSYVAGIAWFLALAFPPLTQRTYMSENAMGSTMV 60
           MGLLSDPVRRRALAR+VLRLN PLCVLSYVAGIAWFLAL FPPLTQRTYMSENAMGSTMV
Sbjct: 1   MGLLSDPVRRRALARLVLRLNAPLCVLSYVAGIAWFLALVFPPLTQRTYMSENAMGSTMV 60

Query: 61  EEQFVGGDRARSFARDFAAHRKKPGALPVAWLERSMRSVGLEVYTQSFSRKLPFPDETHE 120
           EEQF GGDRAR+FARDFAAHRKK GALPVAWLER+MRSVGLEVYTQSFSRKLPFPDETHE
Sbjct: 61  EEQFAGGDRARAFARDFAAHRKKSGALPVAWLERTMRSVGLEVYTQSFSRKLPFPDETHE 120

Query: 121 RYMVSGTNVYGILRAPRSASTESLVLTVPCGPDATNSQAVGLLLALAAHFRGQIYWAKDI 180
           RYMVSGTNVYGILRAPR+ASTESLVLTVPCG D+TNSQAVGLLLALAAHFRGQIYWAKDI
Sbjct: 121 RYMVSGTNVYGILRAPRAASTESLVLTVPCGSDSTNSQAVGLLLALAAHFRGQIYWAKDI 180

Query: 181 IFLVTDHDLLGTEAWLEAYHDINVTGIQSSPLQGRAGAIQAAVALELSSDVVTSLDVTVE 240
           +FLVT+HDLLGTEAWLEAYHD+NVTG+QSSPLQGRAGAIQAAVALELSSDVVTSLDV VE
Sbjct: 181 VFLVTEHDLLGTEAWLEAYHDVNVTGMQSSPLQGRAGAIQAAVALELSSDVVTSLDVAVE 240

Query: 241 GLNGQLPNLDLLNLFQTFCQKGGLLCTLQGKLQPQDWTSLEGPLQGLQTLLLMVLRQASG 300
           GLNGQLPNLDLLNLFQTFCQKGGLLCTLQGKLQP+DWTSL+GPLQGLQTLLLMVLRQASG
Sbjct: 241 GLNGQLPNLDLLNLFQTFCQKGGLLCTLQGKLQPEDWTSLDGPLQGLQTLLLMVLRQASG 300

Query: 301 RPHGPHGLFLRYGVEALTLRGINSFRQYKYDLATVGKALEGMFRKLNHLLERLHQSFFFY 360
           RPHG HGLFLRY VEALTLRGINSFRQYKYDL  VGKALEGMFRKLNHLLERLHQSFF Y
Sbjct: 301 RPHGSHGLFLRYRVEALTLRGINSFRQYKYDLVAVGKALEGMFRKLNHLLERLHQSFFLY 360

Query: 361 LLPALSRFVSIGLYMPATGFLLLVLGLKALELWMQLHQAGVNPEEAGKAPSPGTPLLPTQ 420
           LLP LSRFVSIGLYMPA GFLLLVLGLKALELWMQLH+AG+  EE G AP P  PL P+Q
Sbjct: 361 LLPGLSRFVSIGLYMPAVGFLLLVLGLKALELWMQLHEAGMGLEEPGGAPGPSVPLPPSQ 420

Query: 421 GVGLASLTAPLLISQAMGLALYFLPVLGQHLATQHFPVAEAEAVVLTLLAIYVAGLALPH 480
           GVGLASL APLLISQAMGLALY LPVLGQH+ATQHFPVAEAEAVVLTLLAIY AGLALPH
Sbjct: 421 GVGLASLVAPLLISQAMGLALYVLPVLGQHVATQHFPVAEAEAVVLTLLAIYAAGLALPH 480

Query: 481 NTHRVVNSQVPDRGWMALKLVALIYLALQLGCIALLNFSLGFLLAATMVPAAALAKPHGP 540
           NTHRVV++Q PDRGWMALKLVALIYLALQLGCIAL NFSLGFLLA TMVP AALAKPHGP
Sbjct: 481 NTHRVVSTQAPDRGWMALKLVALIYLALQLGCIALTNFSLGFLLATTMVPTAALAKPHGP 540

Query: 541 RTLYAALLVVTSPAVTLFGSLFLWRELLEVPLSLAEGWQLFLTALAQGVLEHYTYGALLF 600
           RTLYAALLV+TSPA TL GSLFLWREL E PLSLAEGWQLFL ALAQGVLEH+TYGALLF
Sbjct: 541 RTLYAALLVLTSPAATLLGSLFLWRELQEAPLSLAEGWQLFLAALAQGVLEHHTYGALLF 600

Query: 601 PILALGLYPCWLLFWNVLFWK 621
           P+L+LGLYPCWLLFWNVLFWK
Sbjct: 601 PLLSLGLYPCWLLFWNVLFWK 621
>gb|AAF46094.1| (AE003435) CG3033 gene product [Drosophila melanogaster]
          Length = 674

 Score =  727 bits (1858), Expect = 0.0
 Identities = 221/666 (33%), Positives = 315/666 (47%), Gaps = 72/666 (10%)

Query: 1   MGLLSDP--VRRRALARIVLRLNTPLCVLSYVAGIAWFLALAFPPLTQRTYMSENAMGST 58
           MGLLSDP    +  L   + R    +C   YVAG+AWF  LA P     TY+SENA+   
Sbjct: 1   MGLLSDPSISTQSKLVDGLARHVRKVCYALYVAGVAWFFCLALPEFNHGTYLSENALSPG 60

Query: 59  MVEEQF-VGGDR-ARSFARDFAAHRKKP-GALPVAWLERSMRSVGLEVYTQSFSRKLPF- 114
           +V  +  +  +R A     +    RK      P AW+   M   GLE +T +++ + PF 
Sbjct: 61  LVYPEIRIDANRLAIQLLEELQRERKDHLSTTPHAWIAAKMNEFGLETHTHNYTLRYPFG 120

Query: 115 -PDETHERYMVSGTNVYGILRAPRSASTESLVLTVPCGPDATN----SQAVGLLLALAAH 169
              E H      G N+YGILRAPR ASTE +V   P    ++     S +V LLLA A  
Sbjct: 121 GGKEYH------GKNIYGILRAPRIASTEGIVFAAPYRAASSVHTDISASVPLLLAFADF 174

Query: 170 FRGQIYWAKDIIFLVTDHDLLGTEAWLEAYH------DINVTGIQSSPLQGRAGAIQAAV 223
            R + YWAKD+IFL+T+ + LG +AWLEAYH      D++   ++   L  RAG++QAA+
Sbjct: 175 ARRKNYWAKDLIFLITEQEQLGMQAWLEAYHDGDRELDLSKAYLRPGNLPARAGSLQAAL 234

Query: 224 ALELSSDVVTSLDVTVEGLNGQLPNLDLLNLFQTFCQKGGLLCTLQGKLQPQDWTSLEGP 283
            +E+    +  +DV +EGLNG+LPNLD+ NL Q    + G+    +   + +   S    
Sbjct: 235 NIEVQDLEIDHVDVRIEGLNGKLPNLDMFNLVQRIMAREGIASGYKQAPRKKRRHSQSHF 294

Query: 284 LQGLQTLLLMVLRQASGRPHGPHGLFLRYGVEALTL----RGINSFRQYKYDLATVG--K 337
            Q  + +L M+  Q+SG P G HGLF RY ++ALT+    R  ++  +     A V   K
Sbjct: 295 EQNFRQMLTMLASQSSGVPTGNHGLFHRYRIDALTIAANRRATHATLKGSPGSAAVPLLK 354

Query: 338 ALEGMFRKLNHLLERLHQSFFFYLLPALSRFVSIGLYMPATGFLLLVLGLKALELWMQLH 397
           A+EG+ R LN+LLER HQSFFFY++ +  R++SIG YMPA   L+    LKA   W  L 
Sbjct: 355 AIEGIARSLNNLLERFHQSFFFYVIVSNDRYISIGDYMPALVALVACAFLKAYLTWSTLP 414

Query: 398 QAGVNPEEAGK-------------APSPGTPLLPTQGVGLASLTAPLLI---SQAMGLAL 441
                 E+A                  P    LP   V L  LTA LLI      + L  
Sbjct: 415 ATNAELEKAAGWLREHEQEAELEENLEPDKFELPYGSV-LIYLTATLLIGFLCNVLPLQQ 473

Query: 442 YFLPVLGQHLATQHFPVAEAEAVVLTLLAIYVAGLALPHNTHRVVNSQVPDRGWMALKLV 501
           YFL +          P+  A      L  + + G  LP          +P  G   L + 
Sbjct: 474 YFLEI----------PMGAAPLTTSVLSFLSLIGFVLPFVVV------LPPGGLELLHVA 517

Query: 502 ALIYLALQLGCIALLNFSLGFLLAATMVP--AAALAKPHGPR-----TLYAALLVVTSPA 554
            L+     L  I LLNF+LG   A   VP   A   K    R     T+  A LV+    
Sbjct: 518 FLLIYGCALIVIGLLNFALGLFAAVLTVPLVIALETKEENSRSTLRNTIRLATLVMNPMM 577

Query: 555 VTLFGSLFL-WRELLEVPLS--LAEGWQLFLTALAQGVLEHYTYGALLFPILALGLYPCW 611
           V     L + + +  E+P+   +       + A A G+++   YG  L+ ++     P W
Sbjct: 578 VVYVIVLAMTFYQFPELPVQKIMLRAATAAMDASAYGLIDSVIYGNWLYFVICTIFLPLW 637

Query: 612 LLFWNV 617
           ++ W +
Sbjct: 638 IICWTL 643
>ref|NP_491700.1| (NM_059299) F33D11.9b.p [Caenorhabditis elegans]
 gb|AAK68360.1|AF039720_13 (AF039720) Hypothetical protein F33D11.9b [Caenorhabditis elegans]
          Length = 670

 Score =  714 bits (1825), Expect = 0.0
 Identities = 143/644 (22%), Positives = 249/644 (38%), Gaps = 82/644 (12%)

Query: 9   RRRALARIVLRLNTPLCVLSYVAGIAWFLALAFPPLTQRTYMSENAMGSTMVEEQFVGGD 68
            R  +   +L+      +++Y+        L  P   + T +SE+++   +V  +F    
Sbjct: 28  TRPKILDKILKFRKVALIIAYIGAYLSAQWLFDPVNMEVTRISEHSLMPGLVTPKFDKSG 87

Query: 69  RARSFARDFAAHRKKPGALPVAWLERSMRSVGLEVYTQSFSRKLPFPDETHERYMVSGTN 128
            A    R F+   K      +  +      +GLE +T  +  K+           ++G N
Sbjct: 88  IAIQLYRRFSDLPKSKSQQEL--IHTIFSDLGLECFTHKWRSKVAGNP-------MNGEN 138

Query: 129 VYGILRAPRSASTESLVLTVPCGPDATNSQAVGLLLALAAHFRGQIYWAKDIIFLVTD-- 186
           VYG +R PR+   E+ ++ V  G    + + +  +LA   + + Q+YWA+DI+ +  D  
Sbjct: 139 VYGFIRGPRNDGAEAQMIVVQLGRSEKSRRMMSRMLAFVDYAKDQVYWARDIVIVFVDGG 198

Query: 187 -------HDLLGTEAWLEAYHDINVTG------IQSSPLQGRAGAIQAAVALELSSDVVT 233
                        +A+L  Y  I          +++  +Q + GA+   V  +LS   V 
Sbjct: 199 EKNDSIEKAAFALDAFLLKYQKIEALNSKKSITVEADEIQAQTGALIGGVVYDLSGMAVK 258

Query: 234 S---LDVTVEGLNGQLPNLDLLNLFQTFC-----QKGGLLCTLQGKLQP-QDWTSLEGPL 284
               +++   GLNGQ  NLD+ N            +  +  TL     P  +++    P+
Sbjct: 259 GQHIVNIQTNGLNGQQVNLDVFNGITKIADSKHHSRVAIFGTLHRHSNPYMEYSPFIVPM 318

Query: 285 QGLQTLLLMVLRQASGRPHGPHGLFLRYGVEALTLRGINSFRQYKYDLATVGKALEGMFR 344
           + L T       QA     G H +  +YGV+ LT+        + Y     G+ +E + R
Sbjct: 319 RALYT-------QAFISIEGIHSVLGKYGVQGLTVG-----LSHDYSEKQTGQFIEEVSR 366

Query: 345 KLNHLLERLHQSFFFYLLPALSRFVSIGLYMPATGFLLLVLGLKALELWMQLHQAGVNPE 404
            LN++LERLHQS+F Y+L     F SI +Y+     L+  L + A   W ++ +      
Sbjct: 367 MLNNVLERLHQSYFMYVLADDLHFSSIAMYIIPLTILVSPLVISAYFEWTKIQE------ 420

Query: 405 EAGKAPSPGTPLLPTQGVGLASLTAPLLISQAMG----LALYFLPVLGQHLATQHF-PVA 459
              + P      L    +G     A   + Q +G    L     P +G       F P  
Sbjct: 421 --FQFP---VVHLAFHVLGFFYYIATTYVFQTIGTTFVLQWMQWPFVGIDGCDGQFTPSW 475

Query: 460 EAEAVVLTLLAIYVAGLALPHNTHRVVNSQVPDRGWMALKLVALIYLALQLGCIALLNFS 519
                 L    I +          R V S         +++V L+  +L L  I L NF 
Sbjct: 476 SLINSFLIFFMIPLGPYVFLKEPVRCVPS---------MRIVCLLETSLVLYGICLTNFG 526

Query: 520 LGFLLAATMVPAAALAKPHGPRTLY--AALLVVTSPAV---TLFGSLFLWRELLEVPLSL 574
           L  L  A +VP   L      R+++  + L ++   +V          ++ +L   P   
Sbjct: 527 LAILCTAFVVPIVFL---MTTRSIFRSSVLAILNPISVLAFVAINIPGMFGQL--FPTIS 581

Query: 575 AEGWQLFLTALA-QGVLEHYTYGALLFPILALGLYPCW-LLFWN 616
            + W     ++A   + E   +    F +L    YP W +LF  
Sbjct: 582 QDSWLAAAQSIALTAIREDVEFKVNHFFLLCCFGYPIWNMLFAC 625
>pir||T46923 hypothetical protein DKFZp434E1512.1 - human
 emb|CAB75660.1| (AL157437) hypothetical protein [Homo sapiens]
 gb|AAH06383.1|AAH06383 (BC006383) Unknown (protein for MGC:12663) [Homo sapiens]
          Length = 499

 Score =  682 bits (1743), Expect = 0.0
 Identities = 452/499 (90%), Positives = 470/499 (93%)

Query: 123 MVSGTNVYGILRAPRSASTESLVLTVPCGPDATNSQAVGLLLALAAHFRGQIYWAKDIIF 182
           MVSGTNVYGILRAPR+ASTESLVLTVPCG D+TNSQAVGLLLALAAHFRGQIYWAKDI+F
Sbjct: 1   MVSGTNVYGILRAPRAASTESLVLTVPCGSDSTNSQAVGLLLALAAHFRGQIYWAKDIVF 60

Query: 183 LVTDHDLLGTEAWLEAYHDINVTGIQSSPLQGRAGAIQAAVALELSSDVVTSLDVTVEGL 242
           LVT+HDLLGTEAWLEAYHD+NVTG+QSSPLQGRAGAIQAAVALELSSDVVTSLDV VEGL
Sbjct: 61  LVTEHDLLGTEAWLEAYHDVNVTGMQSSPLQGRAGAIQAAVALELSSDVVTSLDVAVEGL 120

Query: 243 NGQLPNLDLLNLFQTFCQKGGLLCTLQGKLQPQDWTSLEGPLQGLQTLLLMVLRQASGRP 302
           NGQLPNLDLLNLFQTFCQKGGLLCTLQGKLQP+DWTSL+GPLQGLQTLLLMVLRQASGRP
Sbjct: 121 NGQLPNLDLLNLFQTFCQKGGLLCTLQGKLQPEDWTSLDGPLQGLQTLLLMVLRQASGRP 180

Query: 303 HGPHGLFLRYGVEALTLRGINSFRQYKYDLATVGKALEGMFRKLNHLLERLHQSFFFYLL 362
           HG HGLFLRY VEALTLRGINSFRQYKYDL  VGKALEGMFRKLNHLLERLHQSFF YLL
Sbjct: 181 HGSHGLFLRYRVEALTLRGINSFRQYKYDLVAVGKALEGMFRKLNHLLERLHQSFFLYLL 240

Query: 363 PALSRFVSIGLYMPATGFLLLVLGLKALELWMQLHQAGVNPEEAGKAPSPGTPLLPTQGV 422
           P LSRFVSIGLYMPA GFLLLVLGLKALELWMQLH+AG+  EE G AP P  PL P+QGV
Sbjct: 241 PGLSRFVSIGLYMPAVGFLLLVLGLKALELWMQLHEAGMGLEEPGGAPGPSVPLPPSQGV 300

Query: 423 GLASLTAPLLISQAMGLALYFLPVLGQHLATQHFPVAEAEAVVLTLLAIYVAGLALPHNT 482
           GLASL APLLISQAMGLALY LPVLGQH+ATQHFPVAEAEAVVLTLLAIY AGLALPHNT
Sbjct: 301 GLASLVAPLLISQAMGLALYVLPVLGQHVATQHFPVAEAEAVVLTLLAIYAAGLALPHNT 360

Query: 483 HRVVNSQVPDRGWMALKLVALIYLALQLGCIALLNFSLGFLLAATMVPAAALAKPHGPRT 542
           HRVV++Q PDRGWMALKLVALIYLALQLGCIAL NFSLGFLLA TMVP AALAKPHGPRT
Sbjct: 361 HRVVSTQAPDRGWMALKLVALIYLALQLGCIALTNFSLGFLLATTMVPTAALAKPHGPRT 420

Query: 543 LYAALLVVTSPAVTLFGSLFLWRELLEVPLSLAEGWQLFLTALAQGVLEHYTYGALLFPI 602
           LYAALLV+TSPA TL GSLFLWREL E PLSLAEGWQLFL ALAQGVLEH+TYGALLFP+
Sbjct: 421 LYAALLVLTSPAATLLGSLFLWRELQEAPLSLAEGWQLFLAALAQGVLEHHTYGALLFPL 480

Query: 603 LALGLYPCWLLFWNVLFWK 621
           L+LGLYPCWLLFWNVLFWK
Sbjct: 481 LSLGLYPCWLLFWNVLFWK 499
>ref|NP_572273.1| (NM_132045) CG3033 gene product [Drosophila melanogaster]
 gb|AAK77275.1| (AY047543) GH05723p [Drosophila melanogaster]
          Length = 561

 Score =  648 bits (1654), Expect = 0.0
 Identities = 203/581 (34%), Positives = 279/581 (47%), Gaps = 64/581 (11%)

Query: 1   MGLLSDP--VRRRALARIVLRLNTPLCVLSYVAGIAWFLALAFPPLTQRTYMSENAMGST 58
           MGLLSDP    +  L   + R    +C   YVAG+AWF  LA P     TY+SENA+   
Sbjct: 1   MGLLSDPSISTQSKLVDGLARHVRKVCYALYVAGVAWFFCLALPEFNHGTYLSENALSPG 60

Query: 59  MVEEQF-VGGDR-ARSFARDFAAHRKKP-GALPVAWLERSMRSVGLEVYTQSFSRKLPF- 114
           +V  +  +  +R A     +    RK      P AW+   M   GLE +T +++ + PF 
Sbjct: 61  LVYPEIRIDANRLAIQLLEELQRERKDHLSTTPHAWIAAKMNEFGLETHTHNYTLRYPFG 120

Query: 115 -PDETHERYMVSGTNVYGILRAPRSASTESLVLTVPCGPDATN----SQAVGLLLALAAH 169
              E H      G N+YGILRAPR ASTE +V   P    ++     S +V LLLA A  
Sbjct: 121 GGKEYH------GKNIYGILRAPRIASTEGIVFAAPYRAASSVHTDISASVPLLLAFADF 174

Query: 170 FRGQIYWAKDIIFLVTDHDLLGTEAWLEAYH------DINVTGIQSSPLQGRAGAIQAAV 223
            R + YWAKD+IFL+T+ + LG +AWLEAYH      D++   ++   L  RAG++QAA+
Sbjct: 175 ARRKNYWAKDLIFLITEQEQLGMQAWLEAYHDGDRELDLSKAYLRPGNLPARAGSLQAAL 234

Query: 224 ALELSSDVVTSLDVTVEGLNGQLPNLDLLNLFQTFCQKGGLLCTLQGKLQPQDWTSLEGP 283
            +E+    +  +DV +EGLNG+LPNLD+ NL Q    + G+    +   + +   S    
Sbjct: 235 NIEVQDLEIDHVDVRIEGLNGKLPNLDMFNLVQRIMAREGIASGYKQAPRKKRRHSQSHF 294

Query: 284 LQGLQTLLLMVLRQASGRPHGPHGLFLRYGVEALTL----RGINSFRQYKYDLATVG--K 337
            Q  + +L M+  Q+SG P G HGLF RY ++ALT+    R  ++  +     A V   K
Sbjct: 295 EQNFRQMLTMLASQSSGVPTGNHGLFHRYRIDALTIAANRRATHATLKGSPGSAAVPLLK 354

Query: 338 ALEGMFRKLNHLLERLHQSFFFYLLPALSRFVSIGLYMPATGFLLLVLGLKALELWMQLH 397
           A+EG+ R LN+LLER HQSFFFY++ +  R++SIG YMPA   L+    LKA   W  L 
Sbjct: 355 AIEGIARSLNNLLERFHQSFFFYVIVSNDRYISIGDYMPALVALVACAFLKAYLTWSTLP 414

Query: 398 QAGVNPEEAGK-------------APSPGTPLLPTQGVGLASLTAPLLI---SQAMGLAL 441
                 E+A                  P    LP   V L  LTA LLI      + L  
Sbjct: 415 ATNAELEKAAGWLREHEQEAELEENLEPDKFELPYGSV-LIYLTATLLIGFLCNVLPLQQ 473

Query: 442 YFLPVLGQHLATQHFPVAEAEAVVLTLLAIYVAGLALPHNTHRVVNSQVPDRGWMALKLV 501
           YFL +          P+  A      L  + + G  LP          +P  G   L + 
Sbjct: 474 YFLEI----------PMGAAPLTTSVLSFLSLIGFVLPFVVV------LPPGGLELLHVA 517

Query: 502 ALIYLALQLGCIALLNFSLGFLLAATMVP--AAALAKPHGP 540
            L+     L  I LLNF+LG   A   VP   A   K    
Sbjct: 518 FLLIYGCALIVIGLLNFALGLFAAVLTVPLVIALETKEENS 558
>ref|NP_197414.1| (NM_121918) GPAA1 - like protein [Arabidopsis thaliana]
          Length = 696

 Score =  634 bits (1619), Expect = e-180
 Identities = 177/698 (25%), Positives = 276/698 (39%), Gaps = 105/698 (15%)

Query: 5   SDPVRRRALARIVLRLNTPLCVLSYV-AGIAWFLALAFPPLTQRTYMSENAMGSTMVEEQ 63
           S  ++ R + R+ + L     V S V +       L  P L + TY+SENA+        
Sbjct: 18  SPKIKPRPIVRLGIFLIAHSPVFSVVFSAAGVLALLLLPLLAKNTYISENALMPGSARSM 77

Query: 64  FVGGDRA--RSFARDFAAHRKKPGALPVAWLERSMRSVGLEVYTQSFSRKLPFPDETHER 121
               D +      +D    R          + + M  +G EV  Q F    P  ++ H  
Sbjct: 78  LSNRDVSDGSKLVKDIKNFRLNHEGQVQKLIGKYMSDMGAEVSYQKFH---PEGNQFHPL 134

Query: 122 YMVS----------------GTNVYGILRAPRSASTESLVLTVPC----GPDATNSQAVG 161
           +  S                G NV GI+RAPR    ES+VL  P     G D  +  ++G
Sbjct: 135 HFFSGPDSYTLLENVSCASYGVNVAGIIRAPRGDGKESIVLVTPYDFINGGDYED-LSLG 193

Query: 162 LLLALAAHFRGQIYWAKDIIFLVTDH---DLLGTEAWLEAYH--DINVTGIQSSPLQG-- 214
           ++ +L +      + +KDII+LV D    D     AWL  YH     V+ +     Q   
Sbjct: 194 IVSSLFSLLSRVTWLSKDIIWLVADSRYGDYRPVAAWLTEYHSPSFKVSDLLKCDEQNTA 253

Query: 215 ----RAGAIQAAVALEL---SSDVVTSLDVTVEGLNGQLPNLDLLNLF------------ 255
               RAG + AA+ L++   S     +L +  E  NGQ+PNLDL+N+             
Sbjct: 254 DNFRRAGTMAAALVLKVDGRSEKFEDTLSIYAEASNGQMPNLDLINVVNYLAVHRQGFYV 313

Query: 256 --------------QTFCQKGGLLCTLQGKLQPQDWT---SLEGPLQGLQTLLLMVLRQA 298
                         + F +    +  L   L P DW         L+G  TL   +  QA
Sbjct: 314 KVEKVVSLLSSSWLKIFGEIFEAVGKLAHMLNP-DWNFGIPAADYLEGSATLASSLYSQA 372

Query: 299 SGRPHGPHGLFLRYGVEALTLRGINSF------RQYKYDLATVGKALEGMFRKLNHLLER 352
            G P GPHG F  Y V+A+TL+    F      RQ+ + L    + LEG  R +N+LLE+
Sbjct: 373 LGIPTGPHGAFRDYQVDAITLKVSPRFPPDSKTRQHDFFLRG-ARLLEGTIRSVNNLLEK 431

Query: 353 LHQSFFFYLLPALSRFVSIGLYMPATGFLLLVLGLKALELWM----QLHQAGVNPEEAGK 408
            HQSFF Y+L + S+F+S+G+YM A   L+  L + A  L++     L  +  NP E  K
Sbjct: 432 FHQSFFLYMLTSPSKFISVGVYMIAFALLVAPLPMVAASLYIDGCKSLTNSTHNPTENFK 491

Query: 409 A---PSPGTPLLPTQGVGLASLTAPLLISQAMGLALYFLPVLGQHLATQHFPVAEAEAVV 465
           +         +     +G      P  I Q          V GQH  T    +    +  
Sbjct: 492 SWKWLDAAKQVFTLHLLGFIVTLLPYFICQ----------VPGQHSPTNRSIMWGTTSSS 541

Query: 466 LTLLAIYVAGLALPHNTHRVVNSQVPDRGWMALKLVALIYLALQLGCIALLNFSLGFLLA 525
           L L+         P       +S++    W  LK V +    + L  ++++NF+   + A
Sbjct: 542 LLLITFVTMPGCSPF------SSRLHGTNWAILKSVTISAAFIGLCLMSIINFATAEIGA 595

Query: 526 ATMVPAAALAKPHGP----RTLYAALLVVTSPAVTLFGSLFLWRELLEVPLSLAEGWQLF 581
             +VP   +A+P  P    R L + L    S  +   G   ++  + +  L         
Sbjct: 596 LLLVPTCLMAQPIKPALRSRRLKSLLGAFCSIVLLTIGFPVMFFAISKGLLGEGLVGLSL 655

Query: 582 LTALAQGVLEHYTYGALLFPILALGLYPCWLLFWNVLF 619
                  +   + + +  +  + +   PCWLL   +LF
Sbjct: 656 GGEFWTWLESLWAWKSATYLYIGMVHLPCWLLCLCILF 693
>pir||T32771 hypothetical protein F33D11.9b - Caenorhabditis elegans
          Length = 885

 Score =  587 bits (1499), Expect = e-166
 Identities = 112/511 (21%), Positives = 197/511 (37%), Gaps = 61/511 (11%)

Query: 9   RRRALARIVLRLNTPLCVLSYVAGIAWFLALAFPPLTQRTYMSENAMGSTMVEEQFVGGD 68
            R  +   +L+      +++Y+        L  P   + T +SE+++   +V  +F    
Sbjct: 290 TRPKILDKILKFRKVALIIAYIGAYLSAQWLFDPVNMEVTRISEHSLMPGLVTPKFDKSG 349

Query: 69  RARSFARDFAAHRKKPGALPVAWLERSMRSVGLEVYTQSFSRKLPFPDETHERYMVSGTN 128
            A    R F+   K      +  +      +GLE +T  +  K+           ++G N
Sbjct: 350 IAIQLYRRFSDLPKSKSQQEL--IHTIFSDLGLECFTHKWRSKVAGNP-------MNGEN 400

Query: 129 VYGILRAPRSASTESLVLTVPCGPDATNSQAVGLLLALAAHFRGQIYWAKDIIFLVTD-- 186
           VYG +R PR+   E+ ++ V  G    + + +  +LA   + + Q+YWA+DI+ +  D  
Sbjct: 401 VYGFIRGPRNDGAEAQMIVVQLGRSEKSRRMMSRMLAFVDYAKDQVYWARDIVIVFVDGG 460

Query: 187 -------HDLLGTEAWLEAYHDINVTG------IQSSPLQGRAGAIQAAVALELSSDVVT 233
                        +A+L  Y  I          +++  +Q + GA+   V  +LS   V 
Sbjct: 461 EKNDSIEKAAFALDAFLLKYQKIEALNSKKSITVEADEIQAQTGALIGGVVYDLSGMAVK 520

Query: 234 S---LDVTVEGLNGQLPNLDLLNLFQTFC-----QKGGLLCTLQGKLQP-QDWTSLEGPL 284
               +++   GLNGQ  NLD+ N            +  +  TL     P  +++    P+
Sbjct: 521 GQHIVNIQTNGLNGQQVNLDVFNGITKIADSKHHSRVAIFGTLHRHSNPYMEYSPFIVPM 580

Query: 285 QGLQTLLLMVLRQASGRPHGPHGLFLRYGVEALTLRGINSFRQYKYDLATVGKALEGMFR 344
           + L T       QA     G H +  +YGV+ LT+        + Y     G+ +E + R
Sbjct: 581 RALYT-------QAFISIEGIHSVLGKYGVQGLTVG-----LSHDYSEKQTGQFIEEVSR 628

Query: 345 KLNHLLERLHQSFFFYLLPALSRFVSIGLYMPATGFLLLVLGLKALELWMQLHQAGVNPE 404
            LN++LERLHQS+F Y+L     F SI +Y+     L+  L + A   W ++ +      
Sbjct: 629 MLNNVLERLHQSYFMYVLADDLHFSSIAMYIIPLTILVSPLVISAYFEWTKIQE------ 682

Query: 405 EAGKAPSPGTPLLPTQGVGLASLTAPLLISQAMG----LALYFLPVLGQHLATQHF-PVA 459
              + P      L    +G     A   + Q +G    L     P +G       F P  
Sbjct: 683 --FQFP---VVHLAFHVLGFFYYIATTYVFQTIGTTFVLQWMQWPFVGIDGCDGQFTPSW 737

Query: 460 EAEAVVLTLLAIYVAGLALPHNTHRVVNSQV 490
                 L    I +             +S +
Sbjct: 738 SLINSFLIFFMIPLGPYVFLKTRSIFRSSVL 768
 Score = 96.5 bits (238), Expect = 1e-18
 Identities = 21/110 (19%), Positives = 41/110 (37%), Gaps = 14/110 (12%)

Query: 514 ALLNFSLGFLLAATMVPAAALAKPHGPRTLY--AALLVVTSPAV---TLFGSLFLWRELL 568
           +L+N  L F +     P   L      R+++  + L ++   +V          ++ +L 
Sbjct: 738 SLINSFLIFFMIPLG-PYVFL----KTRSIFRSSVLAILNPISVLAFVAINIPGMFGQL- 791

Query: 569 EVPLSLAEGWQLFLTALA-QGVLEHYTYGALLFPILALGLYPCW-LLFWN 616
             P    + W     ++A   + E   +    F +L    YP W +LF  
Sbjct: 792 -FPTISQDSWLAAAQSIALTAIREDVEFKVNHFFLLCCFGYPIWNMLFAC 840
>ref|NP_013189.1| (NC_001144) ER protein essential for attaching GPI
           (glycosylphosphatidylinositol) to protein; Gaa1p
           [Saccharomyces cerevisiae]
 sp|P39012|GAA1_YEAST GAA1 PROTEIN
 pir||S45053 membrane protein END2 - yeast (Saccharomyces cerevisiae)
 emb|CAA55944.1| (X79409) Gaa1 [Saccharomyces cerevisiae]
 gb|AAB67592.1| (U53880) Gaa1p [Saccharomyces cerevisiae]
 emb|CAA97649.1| (Z73260) ORF YLR088w [Saccharomyces cerevisiae]
          Length = 614

 Score =  533 bits (1359), Expect = e-150
 Identities = 150/543 (27%), Positives = 251/543 (45%), Gaps = 69/543 (12%)

Query: 1   MGLLSDPVRRRALARIVLRLNTPLCVLSYVAGIAWFLALAFPPLT---QRTYMSENAMGS 57
           M LL    RR     +V R+   L V+S +  +  F+++A  P+    +RTY+SENA+  
Sbjct: 1   MALLEKLHRRIVDMGLVPRIIALLPVISMLCALFGFISIAILPMDGQYRRTYISENALMP 60

Query: 58  TMVEEQFVGGDRARSFARDFAAHRKKPGALPVAWLERSMRSVGLEVYTQSFSRKLPFPDE 117
           +     F   +   +  R + +  K+   + +  +ER+     +  + Q F  K    + 
Sbjct: 61  SQAYSYF--RESEWNILRGYRSQIKE--MVNMTSMERNNL---MGSWLQEFGTKTAIYE- 112

Query: 118 THERYMVSGTNVYGILRAPRSASTESLVLTVPC--GPDATNSQAVGLLLALAAHFRGQIY 175
            +E+Y   G  +YG++ APR   TE++VL VP     D  N     L ++LA  F     
Sbjct: 113 -NEQY---GETLYGVMHAPRGDGTEAMVLAVPWFNSDDEFNIGGAALGVSLARFFSRWPV 168

Query: 176 WAKDIIFLVTDHDLLGTEAWLEAYH-DINVTGIQSSPLQGRAGAIQAAVALELSS--DVV 232
           W+K+II + +++      +W+EAYH  +++TG          G+I+AAV L+ SS  D  
Sbjct: 169 WSKNIIVVFSENPRAALRSWVEAYHTSLDLTG----------GSIEAAVVLDYSSTEDFF 218

Query: 233 TSLDVTVEGLNGQLPNLDLLNLFQTFCQKGGLLCTLQGKLQPQDWTSLEGPLQGLQTLLL 292
             ++++ +GLNG+LPNLDL+N+  +  +  G+  +L G   P D  +       L+ L L
Sbjct: 219 EYVEISYDGLNGELPNLDLVNIAISITEHEGMKVSLHG--LPSDQLTNNNFWSRLKILCL 276

Query: 293 MVLRQASGRPHGPHG--LFLRYGVEALTLRGINSFRQYKYDLATVGKALEGMFRKLNHLL 350
            +   A      PHG   F  + ++++TL+   +     +D+ T G+  E MFR +N+LL
Sbjct: 277 GIRDWALSGVKKPHGNEAFSGWRIQSVTLK---AHGNSGHDITTFGRIPEAMFRSINNLL 333

Query: 351 ERLHQSFFFYLLPALSRFVSIGLYMPATGFLLLVLGLKALELWMQLHQAGVNPEEAGKAP 410
           E+ HQSFFFYLL A  +FVSI  Y+P+   L +   + +L  ++    A ++        
Sbjct: 334 EKFHQSFFFYLLLAPRQFVSISSYLPSAVALSIAFAISSLNAFINNAYANISLF------ 387

Query: 411 SPGTPLLPTQGVGLASLTAPLLISQAMGLALYFLPVLGQHLATQHFPVAEAEAVVLTLLA 470
                 L    V   SL    ++SQA      FL +                     L+ 
Sbjct: 388 --SEYNLVALLVWFVSLVISFVVSQA------FLLIP----------------SSGLLMT 423

Query: 471 IYVAGLALPHNTHRVVNSQVPDRGWMALKLVALIYLALQLGCIALLNFSLGFLLAATMVP 530
           I +A   LP    R ++   P      LK VA +Y +L    + ++NF++  L+     P
Sbjct: 424 ISMASCFLPLILSRKIHISEPLS--YRLKNVAFLYFSLVSTSLLMINFAMALLIGTLAFP 481

Query: 531 AAA 533
              
Sbjct: 482 MTF 484
>emb|CAB86709.1| (AL163505) hypothetical protein L3640.14 [Leishmania major]
          Length = 464

 Score =  274 bits (694), Expect = 4e-72
 Identities = 68/388 (17%), Positives = 119/388 (30%), Gaps = 49/388 (12%)

Query: 134 RAPRSASTESLVLTVPCGPDATNSQAVGLLLALAAHFRGQIYWAKDIIFLVTDHDLLGTE 193
           R  RS  +E  +  V    D     +V L  AL    R +   A D+ F   +       
Sbjct: 75  RGRRSPGSE--IAAVYVNTDY--PASVSLANALIEVIRRRKSLACDVQFYFVNS------ 124

Query: 194 AWLEAYHDINVTGIQSSPLQGRAGAIQAAVALELSSDVVTSLDVTVEGLNGQLPNLDLLN 253
                          S          +AA+ L LS+     + +   G NG  PN D  N
Sbjct: 125 ---------------SEEWPVPQAYTRAALVLNLSTLYNRHICIDTLGGNGIQPNQDYTN 169

Query: 254 L-FQTFCQKGGLLCTLQGKLQPQDWTSLEGPL------QGLQTLLLMVLRQASGRPHGPH 306
           +  Q       L   L  +       +  G        +    L            H  +
Sbjct: 170 IAVQLAAANQFLPSYLCQRRHRSTDDAHAGFWHYWAYLEASVQLPTRPQPWHIIPTHSIN 229

Query: 307 GLFLRYGVEALTLRGINSFRQYKYDLATVGKALEGMFRKLNHLLERLHQSFFFYLLPALS 366
            +     + +  L   +S   +        + +  +   LN L E+ H S F +L  +L 
Sbjct: 230 TI----AISSDPLYSASSVFPHSQLPDAFAQLVLKIIVSLNGLEEKFHHSTFVWLPVSLW 285

Query: 367 RFVSIGLYMPATGFLLLVLGLKALELWMQLHQAGVNPEEAGKAPSPGTPLLPTQGVGLAS 426
           R+V            +  +       + QL    + P       +P       Q  G  +
Sbjct: 286 RYVEYDHAQFCIILFVASIFSTTYSEY-QLRGISITPLFVTLLLTPVAAAAAFQVAGWWA 344

Query: 427 LTAPLLISQAMGLALYFLPVLGQHLATQHFPVAEAEAVVLTLLAIYVAGLA---LPHNTH 483
                ++S +   +L F  ++G H  +       A A+ L ++     GL          
Sbjct: 345 -----VVSASAAFSLLFRLLMG-HANSGMLLSFNAIALCLLIILQPACGLVAGAAAAIQV 398

Query: 484 RVVNSQVPDRGWMALKLV---ALIYLAL 508
             +++ + +R  MA+      AL Y  +
Sbjct: 399 SFIHTALQNRPAMAVGAAVSSALAYYFI 426
>ref|NP_491701.1| (NM_059300) F33D11.9a.p [Caenorhabditis elegans]
 pir||T32772 hypothetical protein F33D11.9a - Caenorhabditis elegans
 gb|AAB96702.1| (AF039720) Hypothetical protein F33D11.9a [Caenorhabditis elegans]
          Length = 229

 Score =  132 bits (331), Expect = 1e-29
 Identities = 19/123 (15%), Positives = 34/123 (27%), Gaps = 16/123 (13%)

Query: 373 LYMPATGFLLLVLGLKALELWMQLHQAGVNPEEAGKAPSPGTPLLPTQGVGLASLTAPLL 432
           +Y+     L+  L + A   W ++ +         + P      L    +G     A   
Sbjct: 1   MYIIPLTILVSPLVISAYFEWTKIQE--------FQFP---VVHLAFHVLGFFYYIATTY 49

Query: 433 ISQAMG----LALYFLPVLGQHLATQHF-PVAEAEAVVLTLLAIYVAGLALPHNTHRVVN 487
           + Q +G    L     P +G       F P        L    I +             +
Sbjct: 50  VFQTIGTTFVLQWMQWPFVGIDGCDGQFTPSWSLINSFLIFFMIPLGPYVFLKTRSIFRS 109

Query: 488 SQV 490
           S +
Sbjct: 110 SVL 112
 Score = 89.9 bits (221), Expect = 1e-16
 Identities = 21/110 (19%), Positives = 41/110 (37%), Gaps = 14/110 (12%)

Query: 514 ALLNFSLGFLLAATMVPAAALAKPHGPRTLY--AALLVVTSPAV---TLFGSLFLWRELL 568
           +L+N  L F +     P   L      R+++  + L ++   +V          ++ +L 
Sbjct: 82  SLINSFLIFFMIPLG-PYVFL----KTRSIFRSSVLAILNPISVLAFVAINIPGMFGQL- 135

Query: 569 EVPLSLAEGWQLFLTALA-QGVLEHYTYGALLFPILALGLYPCW-LLFWN 616
             P    + W     ++A   + E   +    F +L    YP W +LF  
Sbjct: 136 -FPTISQDSWLAAAQSIALTAIREDVEFKVNHFFLLCCFGYPIWNMLFAC 184
>ref|NP_187166.1| (NM_111387) putative sugar transporter [Arabidopsis thaliana]
 gb|AAF27021.1|AC009177_11 (AC009177) putative sugar transporter [Arabidopsis thaliana]
          Length = 463

 Score = 36.1 bits (83), Expect = 1.6
 Identities = 26/151 (17%), Positives = 45/151 (29%), Gaps = 14/151 (9%)

Query: 423 GLASLTAPLLISQAMGLALYFLPVLGQHLATQHFPVAEAEAV---VLTLLAIYVAGLALP 479
            +  L+  + +      +  F   +G    TQ   + E            +I   G  L 
Sbjct: 26  WMVYLSTIIAVC----GSYEFGTCVGYSAPTQFGIMEELNLSYSQFSVFGSILNMGAVLG 81

Query: 480 HNTHRVVNSQVPDRGWMALKLV------ALIYLALQLGCIALLNFSLGFLLAATMVPAAA 533
             T   ++  +  +G M L  V       +IYLA     +    F  G+           
Sbjct: 82  AITSGKISDFIGRKGAMRLSSVISAIGWLIIYLAKGDVPLDFGRFLTGYGCGTLSFVVPV 141

Query: 534 LAKPHGPRTLYAALLVVTSPAVTLFGSLFLW 564
                 PR L  AL  +    +   G   ++
Sbjct: 142 FIAEISPRKLRGALATLNQLFIV-IGLASMF 171
CPU time:   141.32 user secs.	    3.00 sys. secs	  144.32 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.315    0.169    0.467 

Gapped
Lambda     K      H
   0.270   0.0593    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 329251670
Number of Sequences: 887402
Number of extensions: 13956278
Number of successful extensions: 71168
Number of sequences better than 10.0: 270
Number of HSP's better than 10.0 without gapping: 17
Number of HSP's successfully gapped in prelim test: 253
Number of HSP's that attempted gapping in prelim test: 70937
Number of HSP's gapped (non-prelim): 327
length of query: 621
length of database: 277,845,442
effective HSP length: 55
effective length of query: 566
effective length of database: 229,038,332
effective search space: 129635695912
effective search space used: 129635695912
T: 11
A: 40
X1: 15 ( 6.8 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (20.8 bits)
S2: 76 (33.7 bits)