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GPI Anchor Biosynthesis Report: CAB86709.1 (GAA1 family, Leishmania major)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query=
         (464 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!

 Results of PSI-Blast iteration 1

Distribution of 4 Blast Hits on the Query Sequence

Sequences with E-value BETTER than threshold (0.002)




 emb|CAB86709.1|  (AL163505) hypothetical protein L3640.14 [Leishm...   878  0.0


Sequences with E-value WORSE than threshold 


 gb|AAF46094.1|  (AE003435) CG3033 gene product [Drosophila melano...    40  0.073

 ref|NP_572273.1|  (NM_132045) CG3033 gene product [Drosophila mel...    40  0.075

 gb|AAF54906.1|  (AE003698) CG17319 gene product [Drosophila melan...    34  5.2


Alignments


>emb|CAB86709.1| (AL163505) hypothetical protein L3640.14 [Leishmania major]
          Length = 464

 Score =  878 bits (2246), Expect = 0.0
 Identities = 464/464 (100%), Positives = 464/464 (100%)

Query: 1   MLRRVIFNGARHHGGKLAPFFLVLGIGLLCLLPVLRTRKIYIDENAVGQMFPSADAGSVT 60
           MLRRVIFNGARHHGGKLAPFFLVLGIGLLCLLPVLRTRKIYIDENAVGQMFPSADAGSVT
Sbjct: 1   MLRRVIFNGARHHGGKLAPFFLVLGIGLLCLLPVLRTRKIYIDENAVGQMFPSADAGSVT 60

Query: 61  AYLDPTVHWPQRLVRGRRSPGSEIAAVYVNTDYPASVSLANALIEVIRRRKSLACDVQFY 120
           AYLDPTVHWPQRLVRGRRSPGSEIAAVYVNTDYPASVSLANALIEVIRRRKSLACDVQFY
Sbjct: 61  AYLDPTVHWPQRLVRGRRSPGSEIAAVYVNTDYPASVSLANALIEVIRRRKSLACDVQFY 120

Query: 121 FVNSSEEWPVPQAYTRAALVLNLSTLYNRHICIDTLGGNGIQPNQDYTNIAVQLAAANQF 180
           FVNSSEEWPVPQAYTRAALVLNLSTLYNRHICIDTLGGNGIQPNQDYTNIAVQLAAANQF
Sbjct: 121 FVNSSEEWPVPQAYTRAALVLNLSTLYNRHICIDTLGGNGIQPNQDYTNIAVQLAAANQF 180

Query: 181 LPSYLCQRRHRSTDDAHAGFWHYWAYLEASVQLPTRPQPWHIIPTHSINTIAISSDPLYS 240
           LPSYLCQRRHRSTDDAHAGFWHYWAYLEASVQLPTRPQPWHIIPTHSINTIAISSDPLYS
Sbjct: 181 LPSYLCQRRHRSTDDAHAGFWHYWAYLEASVQLPTRPQPWHIIPTHSINTIAISSDPLYS 240

Query: 241 ASSVFPHSQLPDAFAQLVLKIIVSLNGLEEKFHHSTFVWLPVSLWRYVEYDHAQFCIILF 300
           ASSVFPHSQLPDAFAQLVLKIIVSLNGLEEKFHHSTFVWLPVSLWRYVEYDHAQFCIILF
Sbjct: 241 ASSVFPHSQLPDAFAQLVLKIIVSLNGLEEKFHHSTFVWLPVSLWRYVEYDHAQFCIILF 300

Query: 301 VASIFSTTYSEYQLRGISITPLFVTLLLTPVAAAAAFQVAGWWAVVSASAAFSLLFRLLM 360
           VASIFSTTYSEYQLRGISITPLFVTLLLTPVAAAAAFQVAGWWAVVSASAAFSLLFRLLM
Sbjct: 301 VASIFSTTYSEYQLRGISITPLFVTLLLTPVAAAAAFQVAGWWAVVSASAAFSLLFRLLM 360

Query: 361 GHANSGMLLSFNAIALCLLIILQPACGLVAGAAAAIQVSFIHTALQNRPAMAVGAAVSSA 420
           GHANSGMLLSFNAIALCLLIILQPACGLVAGAAAAIQVSFIHTALQNRPAMAVGAAVSSA
Sbjct: 361 GHANSGMLLSFNAIALCLLIILQPACGLVAGAAAAIQVSFIHTALQNRPAMAVGAAVSSA 420

Query: 421 LAYYFIYHLRLPLFGVDGISELFVSFVIYPNAVWIGSRFLCFLY 464
           LAYYFIYHLRLPLFGVDGISELFVSFVIYPNAVWIGSRFLCFLY
Sbjct: 421 LAYYFIYHLRLPLFGVDGISELFVSFVIYPNAVWIGSRFLCFLY 464





>gb|AAF46094.1| (AE003435) CG3033 gene product [Drosophila melanogaster]
          Length = 674

 Score = 40.3 bits (93), Expect = 0.073
 Identities = 65/260 (25%), Positives = 102/260 (39%), Gaps = 40/260 (15%)

Query: 85  AAVYVNTDYPASVSLANALIEVIRRRKSLACDVQFYFVNSSE---------------EWP 129
           AA  V+TD  ASV L  A  +  RR+   A D+ F      +               E  
Sbjct: 153 AASSVHTDISASVPLLLAFADFARRKNYWAKDLIFLITEQEQLGMQAWLEAYHDGDRELD 212

Query: 130 VPQAYTR------------AALVLNLSTLYNRHICIDTLGGNGIQPNQDYTNIAVQLAAA 177
           + +AY R            AAL + +  L   H+ +   G NG  PN D  N+  ++ A 
Sbjct: 213 LSKAYLRPGNLPARAGSLQAALNIEVQDLEIDHVDVRIEGLNGKLPNLDMFNLVQRIMAR 272

Query: 178 NQFLPSY-LCQRRHRSTDDAH--AGFWHYWAYLEA-SVQLPTRPQPWHIIPTHSINTIAI 233
                 Y    R+ R    +H    F      L + S  +PT      +   + I+ + I
Sbjct: 273 EGIASGYKQAPRKKRRHSQSHFEQNFRQMLTMLASQSSGVPTGNH--GLFHRYRIDALTI 330

Query: 234 SSDPLYSASSVFPHSQLPDAFAQLVLKIIV----SLNGLEEKFHHSTFVWLPVSLWRYVE 289
           +++   + +++      P + A  +LK I     SLN L E+FH S F ++ VS  RY+ 
Sbjct: 331 AANRRATHATL---KGSPGSAAVPLLKAIEGIARSLNNLLERFHQSFFFYVIVSNDRYIS 387

Query: 290 YDHAQFCIILFVASIFSTTY 309
                  ++  VA  F   Y
Sbjct: 388 IGDYMPALVALVACAFLKAY 407





>ref|NP_572273.1| (NM_132045) CG3033 gene product [Drosophila melanogaster]
 gb|AAK77275.1| (AY047543) GH05723p [Drosophila melanogaster]
          Length = 561

 Score = 40.3 bits (93), Expect = 0.075
 Identities = 65/260 (25%), Positives = 102/260 (39%), Gaps = 40/260 (15%)

Query: 85  AAVYVNTDYPASVSLANALIEVIRRRKSLACDVQFYFVNSSE---------------EWP 129
           AA  V+TD  ASV L  A  +  RR+   A D+ F      +               E  
Sbjct: 153 AASSVHTDISASVPLLLAFADFARRKNYWAKDLIFLITEQEQLGMQAWLEAYHDGDRELD 212

Query: 130 VPQAYTR------------AALVLNLSTLYNRHICIDTLGGNGIQPNQDYTNIAVQLAAA 177
           + +AY R            AAL + +  L   H+ +   G NG  PN D  N+  ++ A 
Sbjct: 213 LSKAYLRPGNLPARAGSLQAALNIEVQDLEIDHVDVRIEGLNGKLPNLDMFNLVQRIMAR 272

Query: 178 NQFLPSY-LCQRRHRSTDDAH--AGFWHYWAYLEA-SVQLPTRPQPWHIIPTHSINTIAI 233
                 Y    R+ R    +H    F      L + S  +PT      +   + I+ + I
Sbjct: 273 EGIASGYKQAPRKKRRHSQSHFEQNFRQMLTMLASQSSGVPTGNH--GLFHRYRIDALTI 330

Query: 234 SSDPLYSASSVFPHSQLPDAFAQLVLKIIV----SLNGLEEKFHHSTFVWLPVSLWRYVE 289
           +++   + +++      P + A  +LK I     SLN L E+FH S F ++ VS  RY+ 
Sbjct: 331 AANRRATHATL---KGSPGSAAVPLLKAIEGIARSLNNLLERFHQSFFFYVIVSNDRYIS 387

Query: 290 YDHAQFCIILFVASIFSTTY 309
                  ++  VA  F   Y
Sbjct: 388 IGDYMPALVALVACAFLKAY 407





>gb|AAF54906.1| (AE003698) CG17319 gene product [Drosophila melanogaster]
          Length = 812

 Score = 34.1 bits (77), Expect = 5.2
 Identities = 21/54 (38%), Positives = 27/54 (49%), Gaps = 5/54 (9%)

Query: 187 QRRHRSTDDAHAGFWHYWAYLEASVQLPTRPQPWHIIPTHSINTIAISSDPLYS 240
           QR    TDDAH+ F HY   + ++   P R Q WH    HS    A  S PL++
Sbjct: 644 QRHLNETDDAHS-FLHYQGSVNSNGGDPARLQKWH----HSTRREAPYSSPLHN 692





CPU time:    99.80 user secs.	    2.16 sys. secs	  101.96 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.327    0.139    0.434 

Gapped
Lambda     K      H
   0.270   0.0487    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 268872142
Number of Sequences: 887402
Number of extensions: 11213550
Number of successful extensions: 36032
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 27
Number of HSP's that attempted gapping in prelim test: 36016
Number of HSP's gapped (non-prelim): 36
length of query: 464
length of database: 277,845,442
effective HSP length: 53
effective length of query: 411
effective length of database: 230,813,136
effective search space: 94864198896
effective search space used: 94864198896
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 40 (21.7 bits)
S2: 74 (33.2 bits)