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GPI Anchor Biosynthesis Report: AAF46094.1 (GAA1 family, Drosophila melanogaster)




BLASTP 2.1.1 [Aug-8-2000]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query=
         (674 letters)

Database: nr
           887,402 sequences; 277,845,442 total letters

Searching..................................................

Converged !!!


Results of PSI-Blast iteration 3

Distribution of 17 Blast Hits on the Query Sequence


Sequences with E-value BETTER than threshold (0.002)

gb|AAF46094.1| (AE003435) CG3033 gene product [Drosophila melano... 904 0.0
ref|NP_572273.1| (NM_132045) CG3033 gene product [Drosophila mel... 750 0.0
ref|NP_491700.1| (NM_059299) F33D11.9b.p [Caenorhabditis elegans... 726 0.0
ref|NP_034461.1| (NM_010331) GPI anchor attachment protein 1 [Mu... 708 0.0
ref|NP_003792.1| (NM_003801) anchor attachment protein 1; anchor... 693 0.0
ref|NP_013189.1| (NC_001144) ER protein essential for attaching ... 570 e-161
pir||T46923 hypothetical protein DKFZp434E1512.1 - human >gi|701... 568 e-161
pir||T32771 hypothetical protein F33D11.9b - Caenorhabditis elegans 558 e-157
emb|CAB65611.1| (AL136078) putative glycosylphosphatidylinositol... 534 e-150
ref|NP_197414.1| (NM_121918) GPAA1 - like protein [Arabidopsis t... 310 6e-83
emb|CAB86709.1| (AL163505) hypothetical protein L3640.14 [Leishm... 184 4e-45
ref|NP_491701.1| (NM_059300) F33D11.9a.p [Caenorhabditis elegans... 141 3e-32
Sequences with E-value WORSE than threshold

ref|NP_375951.1| (NC_003106) 815aa long hypothetical quinol oxid... 35 3.6
ref|NP_391656.1| (NC_000964) amino acid permease [Bacillus subti... 34 7.7
Alignments
>gb|AAF46094.1| (AE003435) CG3033 gene product [Drosophila melanogaster]
          Length = 674

 Score =  904 bits (2312), Expect = 0.0
 Identities = 674/674 (100%), Positives = 674/674 (100%)

Query: 1   MGLLSDPSISTQSKLVDGLARHVRKVCYALYVAGVAWFFCLALPEFNHGTYLSENALSPG 60
           MGLLSDPSISTQSKLVDGLARHVRKVCYALYVAGVAWFFCLALPEFNHGTYLSENALSPG
Sbjct: 1   MGLLSDPSISTQSKLVDGLARHVRKVCYALYVAGVAWFFCLALPEFNHGTYLSENALSPG 60

Query: 61  LVYPEIRIDANRLAIQLLEELQRERKDHLSTTPHAWIAAKMNEFGLETHTHNYTLRYPFG 120
           LVYPEIRIDANRLAIQLLEELQRERKDHLSTTPHAWIAAKMNEFGLETHTHNYTLRYPFG
Sbjct: 61  LVYPEIRIDANRLAIQLLEELQRERKDHLSTTPHAWIAAKMNEFGLETHTHNYTLRYPFG 120

Query: 121 GGKEYHGKNIYGILRAPRIASTEGIVFAAPYRAASSVHTDISASVPLLLAFADFARRKNY 180
           GGKEYHGKNIYGILRAPRIASTEGIVFAAPYRAASSVHTDISASVPLLLAFADFARRKNY
Sbjct: 121 GGKEYHGKNIYGILRAPRIASTEGIVFAAPYRAASSVHTDISASVPLLLAFADFARRKNY 180

Query: 181 WAKDLIFLITEQEQLGMQAWLEAYHDGDRELDLSKAYLRPGNLPARAGSLQAALNIEVQD 240
           WAKDLIFLITEQEQLGMQAWLEAYHDGDRELDLSKAYLRPGNLPARAGSLQAALNIEVQD
Sbjct: 181 WAKDLIFLITEQEQLGMQAWLEAYHDGDRELDLSKAYLRPGNLPARAGSLQAALNIEVQD 240

Query: 241 LEIDHVDVRIEGLNGKLPNLDMFNLVQRIMAREGIASGYKQAPRKKRRHSQSHFEQNFRQ 300
           LEIDHVDVRIEGLNGKLPNLDMFNLVQRIMAREGIASGYKQAPRKKRRHSQSHFEQNFRQ
Sbjct: 241 LEIDHVDVRIEGLNGKLPNLDMFNLVQRIMAREGIASGYKQAPRKKRRHSQSHFEQNFRQ 300

Query: 301 MLTMLASQSSGVPTGNHGLFHRYRIDALTIAANRRATHATLKGSPGSAAVPLLKAIEGIA 360
           MLTMLASQSSGVPTGNHGLFHRYRIDALTIAANRRATHATLKGSPGSAAVPLLKAIEGIA
Sbjct: 301 MLTMLASQSSGVPTGNHGLFHRYRIDALTIAANRRATHATLKGSPGSAAVPLLKAIEGIA 360

Query: 361 RSLNNLLERFHQSFFFYVIVSNDRYISIGDYMPALVALVACAFLKAYLTWSTLPATNAEL 420
           RSLNNLLERFHQSFFFYVIVSNDRYISIGDYMPALVALVACAFLKAYLTWSTLPATNAEL
Sbjct: 361 RSLNNLLERFHQSFFFYVIVSNDRYISIGDYMPALVALVACAFLKAYLTWSTLPATNAEL 420

Query: 421 EKAAGWLREHEQEAELEENLEPDKFELPYGSVLIYLTATLLIGFLCNVLPLQQYFLEIPM 480
           EKAAGWLREHEQEAELEENLEPDKFELPYGSVLIYLTATLLIGFLCNVLPLQQYFLEIPM
Sbjct: 421 EKAAGWLREHEQEAELEENLEPDKFELPYGSVLIYLTATLLIGFLCNVLPLQQYFLEIPM 480

Query: 481 GAAPLTTSVLSFLSLIGFVLPFVVVLPPGGLELLHVAFLLIYGCALIVIGLLNFALGLFA 540
           GAAPLTTSVLSFLSLIGFVLPFVVVLPPGGLELLHVAFLLIYGCALIVIGLLNFALGLFA
Sbjct: 481 GAAPLTTSVLSFLSLIGFVLPFVVVLPPGGLELLHVAFLLIYGCALIVIGLLNFALGLFA 540

Query: 541 AVLTVPLVIALETKEENSRSTLRNTIRLATLVMNPMMVVYVIVLAMTFYQFPELPVQKIM 600
           AVLTVPLVIALETKEENSRSTLRNTIRLATLVMNPMMVVYVIVLAMTFYQFPELPVQKIM
Sbjct: 541 AVLTVPLVIALETKEENSRSTLRNTIRLATLVMNPMMVVYVIVLAMTFYQFPELPVQKIM 600

Query: 601 LRAATAAMDASAYGLIDSVIYGNWLYFVICTIFLPLWIICWTLSLSKRRDYADYLDFDES 660
           LRAATAAMDASAYGLIDSVIYGNWLYFVICTIFLPLWIICWTLSLSKRRDYADYLDFDES
Sbjct: 601 LRAATAAMDASAYGLIDSVIYGNWLYFVICTIFLPLWIICWTLSLSKRRDYADYLDFDES 660

Query: 661 PSTSPQPQSKVKTN 674
           PSTSPQPQSKVKTN
Sbjct: 661 PSTSPQPQSKVKTN 674
>ref|NP_572273.1| (NM_132045) CG3033 gene product [Drosophila melanogaster]
 gb|AAK77275.1| (AY047543) GH05723p [Drosophila melanogaster]
          Length = 561

 Score =  750 bits (1918), Expect = 0.0
 Identities = 558/558 (100%), Positives = 558/558 (100%)

Query: 1   MGLLSDPSISTQSKLVDGLARHVRKVCYALYVAGVAWFFCLALPEFNHGTYLSENALSPG 60
           MGLLSDPSISTQSKLVDGLARHVRKVCYALYVAGVAWFFCLALPEFNHGTYLSENALSPG
Sbjct: 1   MGLLSDPSISTQSKLVDGLARHVRKVCYALYVAGVAWFFCLALPEFNHGTYLSENALSPG 60

Query: 61  LVYPEIRIDANRLAIQLLEELQRERKDHLSTTPHAWIAAKMNEFGLETHTHNYTLRYPFG 120
           LVYPEIRIDANRLAIQLLEELQRERKDHLSTTPHAWIAAKMNEFGLETHTHNYTLRYPFG
Sbjct: 61  LVYPEIRIDANRLAIQLLEELQRERKDHLSTTPHAWIAAKMNEFGLETHTHNYTLRYPFG 120

Query: 121 GGKEYHGKNIYGILRAPRIASTEGIVFAAPYRAASSVHTDISASVPLLLAFADFARRKNY 180
           GGKEYHGKNIYGILRAPRIASTEGIVFAAPYRAASSVHTDISASVPLLLAFADFARRKNY
Sbjct: 121 GGKEYHGKNIYGILRAPRIASTEGIVFAAPYRAASSVHTDISASVPLLLAFADFARRKNY 180

Query: 181 WAKDLIFLITEQEQLGMQAWLEAYHDGDRELDLSKAYLRPGNLPARAGSLQAALNIEVQD 240
           WAKDLIFLITEQEQLGMQAWLEAYHDGDRELDLSKAYLRPGNLPARAGSLQAALNIEVQD
Sbjct: 181 WAKDLIFLITEQEQLGMQAWLEAYHDGDRELDLSKAYLRPGNLPARAGSLQAALNIEVQD 240

Query: 241 LEIDHVDVRIEGLNGKLPNLDMFNLVQRIMAREGIASGYKQAPRKKRRHSQSHFEQNFRQ 300
           LEIDHVDVRIEGLNGKLPNLDMFNLVQRIMAREGIASGYKQAPRKKRRHSQSHFEQNFRQ
Sbjct: 241 LEIDHVDVRIEGLNGKLPNLDMFNLVQRIMAREGIASGYKQAPRKKRRHSQSHFEQNFRQ 300

Query: 301 MLTMLASQSSGVPTGNHGLFHRYRIDALTIAANRRATHATLKGSPGSAAVPLLKAIEGIA 360
           MLTMLASQSSGVPTGNHGLFHRYRIDALTIAANRRATHATLKGSPGSAAVPLLKAIEGIA
Sbjct: 301 MLTMLASQSSGVPTGNHGLFHRYRIDALTIAANRRATHATLKGSPGSAAVPLLKAIEGIA 360

Query: 361 RSLNNLLERFHQSFFFYVIVSNDRYISIGDYMPALVALVACAFLKAYLTWSTLPATNAEL 420
           RSLNNLLERFHQSFFFYVIVSNDRYISIGDYMPALVALVACAFLKAYLTWSTLPATNAEL
Sbjct: 361 RSLNNLLERFHQSFFFYVIVSNDRYISIGDYMPALVALVACAFLKAYLTWSTLPATNAEL 420

Query: 421 EKAAGWLREHEQEAELEENLEPDKFELPYGSVLIYLTATLLIGFLCNVLPLQQYFLEIPM 480
           EKAAGWLREHEQEAELEENLEPDKFELPYGSVLIYLTATLLIGFLCNVLPLQQYFLEIPM
Sbjct: 421 EKAAGWLREHEQEAELEENLEPDKFELPYGSVLIYLTATLLIGFLCNVLPLQQYFLEIPM 480

Query: 481 GAAPLTTSVLSFLSLIGFVLPFVVVLPPGGLELLHVAFLLIYGCALIVIGLLNFALGLFA 540
           GAAPLTTSVLSFLSLIGFVLPFVVVLPPGGLELLHVAFLLIYGCALIVIGLLNFALGLFA
Sbjct: 481 GAAPLTTSVLSFLSLIGFVLPFVVVLPPGGLELLHVAFLLIYGCALIVIGLLNFALGLFA 540

Query: 541 AVLTVPLVIALETKEENS 558
           AVLTVPLVIALETKEENS
Sbjct: 541 AVLTVPLVIALETKEENS 558
>ref|NP_491700.1| (NM_059299) F33D11.9b.p [Caenorhabditis elegans]
 gb|AAK68360.1|AF039720_13 (AF039720) Hypothetical protein F33D11.9b [Caenorhabditis elegans]
          Length = 670

 Score =  726 bits (1854), Expect = 0.0
 Identities = 153/671 (22%), Positives = 270/671 (39%), Gaps = 93/671 (13%)

Query: 4   LSDPSISTQSKLVDGLARHVRKVCYALYVAG----VAWFFCLALPEFNHGTYLSENALSP 59
           +S    +T+ K++D + +  RKV   +   G      W F    P     T +SE++L P
Sbjct: 21  ISKDGGTTRPKILDKILK-FRKVALIIAYIGAYLSAQWLFD---PVNMEVTRISEHSLMP 76

Query: 60  GLVYPEIRIDANRLAIQLLEELQRERKDHLSTTPHAWIAAKMNEFGLETHTHNYTLRYPF 119
           GLV P  + D + +AIQL        K   S +    I    ++ GLE  TH +  +   
Sbjct: 77  GLVTP--KFDKSGIAIQLYRRFSDLPK---SKSQQELIHTIFSDLGLECFTHKWRSKV-- 129

Query: 120 GGGKEYHGKNIYGILRAPRIASTEGIVFAAPYRAASSVHTDISASVPLLLAFADFARRKN 179
             G   +G+N+YG +R PR    E  +       +      +S     +LAF D+A+ + 
Sbjct: 130 -AGNPMNGENVYGFIRGPRNDGAEAQMIVVQLGRSEKSRRMMSR----MLAFVDYAKDQV 184

Query: 180 YWAKDLIFLIT---------EQEQLGMQAWLEAYHDGDRELDLSKAYLRPGNLPARAGSL 230
           YWA+D++ +           E+    + A+L  Y   +         +    + A+ G+L
Sbjct: 185 YWARDIVIVFVDGGEKNDSIEKAAFALDAFLLKYQKIEALNSKKSITVEADEIQAQTGAL 244

Query: 231 QAALNIEVQDLEIDH---VDVRIEGLNGKLPNLDMFNLVQRIMAREGIASGYKQAPRKK- 286
              +  ++  + +     V+++  GLNG+  NLD+FN + +I          K   R   
Sbjct: 245 IGGVVYDLSGMAVKGQHIVNIQTNGLNGQQVNLDVFNGITKIA-------DSKHHSRVAI 297

Query: 287 ----RRHSQSHFEQN-FRQMLTMLASQSSGVPTGNHGLFHRYRIDALTIAANRRATHATL 341
                RHS  + E + F   +  L +Q+     G H +  +Y +  LT+      +H   
Sbjct: 298 FGTLHRHSNPYMEYSPFIVPMRALYTQAFISIEGIHSVLGKYGVQGLTVG----LSHDYS 353

Query: 342 KGSPGSAAVPLLKAIEGIARSLNNLLERFHQSFFFYVIVSNDRYISIGDYMPALVALVAC 401
           +           + IE ++R LNN+LER HQS+F YV+  +  + SI  Y+  L  LV+ 
Sbjct: 354 EKQT-------GQFIEEVSRMLNNVLERLHQSYFMYVLADDLHFSSIAMYIIPLTILVSP 406

Query: 402 AFLKAYLTWSTLPATNAELEKAAGWLREHEQEAELEENLEPDKFELPYGSVLIYLTATLL 461
             + AY  W+ +                  Q   +        F     +  ++ T    
Sbjct: 407 LVISAYFEWTKIQE---------------FQFPVVHLAFHVLGFFYYIATTYVFQT---- 447

Query: 462 IGFLCNVLPLQQYFLEI---PMGAAPLTTSVLSFLSLIGFVLPFVVVL----PPGGLELL 514
           IG    +  +Q  F+ I        P  + + SFL  I F++P    +    P   +  +
Sbjct: 448 IGTTFVLQWMQWPFVGIDGCDGQFTPSWSLINSFL--IFFMIPLGPYVFLKEPVRCVPSM 505

Query: 515 HVAFLLIYGCALIVIGLLNFALGLFAAVLTVPLVIALETKEENSRSTLRNTIRLATLVMN 574
            +  LL     L  I L NF L +      VP+V  + T     RS  R+++      ++
Sbjct: 506 RIVCLLETSLVLYGICLTNFGLAILCTAFVVPIVFLMTT-----RSIFRSSVLAILNPIS 560

Query: 575 PMMVVYVIVLAMTFYQFPELPVQKIMLRAATAAMDASAYGLIDSVIYGNWLYFVICTIFL 634
            +  V + +  M    FP +     +  A + A+ A    +   V +    +F++C    
Sbjct: 561 VLAFVAINIPGMFGQLFPTISQDSWLAAAQSIALTAIREDVEFKVNH----FFLLCCFGY 616

Query: 635 PLWIICWTLSL 645
           P+W + +  S+
Sbjct: 617 PIWNMLFACSM 627
>ref|NP_034461.1| (NM_010331) GPI anchor attachment protein 1 [Mus musculus]
 dbj|BAA82589.1| (AB002136) glycosylphosphatidylinositol anchor attachment 1 (GPAA1)
           [Mus musculus]
 dbj|BAA82591.1| (AB002138) glycosylphosphatidylinositol anchor attachment 1 (GPAA1)
           [Mus musculus]
 dbj|BAB03274.1| (AB006970) GPI anchor attachment protein [Mus musculus]
 dbj|BAB03275.1| (AB006971) GPI anchor attachment protein [Mus musculus]
 gb|AAH06697.1|AAH06697 (BC006697) GPI anchor attachment protein 1 [Mus musculus]
          Length = 621

 Score =  708 bits (1808), Expect = 0.0
 Identities = 218/667 (32%), Positives = 310/667 (45%), Gaps = 66/667 (9%)

Query: 1   MGLLSDPSISTQSKLVDGLARHVRKVCYALYVAGVAWFFCLALPEFNHGTYLSENALSPG 60
           MGLLSDP    +  L   + R    +C   YVAG+AWF  LA P     TY+SENA+   
Sbjct: 1   MGLLSDP--VRRRALARIVLRLNTPLCVLSYVAGIAWFLALAFPPLTQRTYMSENAMGST 58

Query: 61  LVYPEIRIDANRLAIQLLEELQRERKDHLSTTPHAWIAAKMNEFGLETHTHNYTLRYPFG 120
           +V  +        A     +    RK      P AW+   M   GLE +T +++ + PF 
Sbjct: 59  MVEEQFVGGDR--ARSFARDFAAHRKKP-GALPVAWLERSMRSVGLEVYTQSFSRKLPF- 114

Query: 121 GGKEYH------GKNIYGILRAPRIASTEGIVFAAPYRAASSVHTDISASVPLLLAFADF 174
              E H      G N+YGILRAPR ASTE +V   P    ++    +   + L   F   
Sbjct: 115 -PDETHERYMVSGTNVYGILRAPRSASTESLVLTVPCGPDATNSQAVGLLLALAAHF--- 170

Query: 175 ARRKNYWAKDLIFLITEQEQLGMQAWLEAYHDGDRELDLSKAYLRPGNLPARAGSLQAAL 234
            R + YWAKD+IFL+T+ + LG +AWLEAYH      D++   ++   L  RAG++QAA+
Sbjct: 171 -RGQIYWAKDIIFLVTDHDLLGTEAWLEAYH------DINVTGIQSSPLQGRAGAIQAAV 223

Query: 235 NIEVQDLEIDHVDVRIEGLNGKLPNLDMFNLVQRIMAREGIASGYKQAPRKKRRHSQSHF 294
            +E+    +  +DV +EGLNG+LPNLD+ NL Q    + G+    +   + +   S    
Sbjct: 224 ALELSSDVVTSLDVTVEGLNGQLPNLDLLNLFQTFCQKGGLLCTLQGKLQPQDWTSLEGP 283

Query: 295 EQNFRQMLTMLASQSSGVPTGNHGLFHRYRIDALTIAANRRATHATLKGSPGSAAVPLLK 354
            Q  + +L M+  Q+SG P G HGLF RY ++ALT+    R  ++  +     A V   K
Sbjct: 284 LQGLQTLLLMVLRQASGRPHGPHGLFLRYGVEALTL----RGINSFRQYKYDLATV--GK 337

Query: 355 AIEGIARSLNNLLERFHQSFFFYVIVSNDRYISIGDYMPALVALVACAFLKAYLTWSTLP 414
           A+EG+ R LN+LLER HQSFFFY++ +  R++SIG YMPA   L+    LKA   W  L 
Sbjct: 338 ALEGMFRKLNHLLERLHQSFFFYLLPALSRFVSIGLYMPATGFLLLVLGLKALELWMQLH 397

Query: 415 ATNAELEKAAGWLREHEQEAELEENLEPDKFELPY-GSVLIYLTATLLI----GFLCNVL 469
                 E+A                  P    LP  G  L  LTA LLI    G     L
Sbjct: 398 QAGVNPEEAGK-------------APSPGTPLLPTQGVGLASLTAPLLISQAMGLALYFL 444

Query: 470 P-LQQYFLEIPMGAAPLTTSVLSFLSL--IGFVLPFVV------VLPPGGLELLHVAFLL 520
           P L Q+        A     VL+ L++   G  LP          +P  G   L +  L+
Sbjct: 445 PVLGQHLATQHFPVAEAEAVVLTLLAIYVAGLALPHNTHRVVNSQVPDRGWMALKLVALI 504

Query: 521 IYGCALIVIGLLNFALGLFAAVLTVPLVIALETKEENSRSTLRNTIRLATLVMNPMMVVY 580
                L  I LLNF+LG   A   VP   A   K    R     T+  A LV+    V  
Sbjct: 505 YLALQLGCIALLNFSLGFLLAATMVP--AAALAKPHGPR-----TLYAALLVVTSPAVTL 557

Query: 581 VIVLAMTFYQFPELPVQKIMLRAATAAMDASAYGLIDSVIYGNWLYFVICTIFLPLWIIC 640
              L + + +  E+P+   +       + A A G+++   YG  L+ ++     P W++ 
Sbjct: 558 FGSLFL-WRELLEVPLS--LAEGWQLFLTALAQGVLEHYTYGALLFPILALGLYPCWLLF 614

Query: 641 WTLSLSK 647
           W +   K
Sbjct: 615 WNVLFWK 621
>ref|NP_003792.1| (NM_003801) anchor attachment protein 1; anchor attachment protein
           1 (Gaa1p, yeast) homolog; glycophosphatidylinositol
           anchor attachment 1 [Homo sapiens]
 dbj|BAA24035.1| (AB006969) hGAA1 [Homo sapiens]
 dbj|BAA82588.1| (AB002135) glycosylphosphatidylinositol anchor attachment 1 (GPAA1)
           [Homo sapiens]
 dbj|BAA82590.1| (AB002137) glycosylphosphatidylinositol anchor attachment 1 (GPAA1)
           [Homo sapiens]
 gb|AAH03171.1|AAH03171 (BC003171) anchor attachment protein 1 (Gaa1p, yeast) homolog [Homo
           sapiens]
 gb|AAH04129.1|AAH04129 (BC004129) anchor attachment protein 1 (Gaa1p, yeast) homolog [Homo
           sapiens]
          Length = 621

 Score =  693 bits (1770), Expect = 0.0
 Identities = 216/666 (32%), Positives = 310/666 (46%), Gaps = 64/666 (9%)

Query: 1   MGLLSDPSISTQSKLVDGLARHVRKVCYALYVAGVAWFFCLALPEFNHGTYLSENALSPG 60
           MGLLSDP    +  L   + R    +C   YVAG+AWF  L  P     TY+SENA+   
Sbjct: 1   MGLLSDP--VRRRALARLVLRLNAPLCVLSYVAGIAWFLALVFPPLTQRTYMSENAMGST 58

Query: 61  LVYPEIRIDANRLAIQLLEELQRERKDHLSTTPHAWIAAKMNEFGLETHTHNYTLRYPFG 120
           +V  +        A        R++   L   P AW+   M   GLE +T +++ + PF 
Sbjct: 59  MVEEQFAGGDRARAFARDFAAHRKKSGAL---PVAWLERTMRSVGLEVYTQSFSRKLPF- 114

Query: 121 GGKEYH------GKNIYGILRAPRIASTEGIVFAAPYRAASSVHTDISASVPLLLAFADF 174
              E H      G N+YGILRAPR ASTE +V   P  + S+    +   + L   F   
Sbjct: 115 -PDETHERYMVSGTNVYGILRAPRAASTESLVLTVPCGSDSTNSQAVGLLLALAAHF--- 170

Query: 175 ARRKNYWAKDLIFLITEQEQLGMQAWLEAYHDGDRELDLSKAYLRPGNLPARAGSLQAAL 234
            R + YWAKD++FL+TE + LG +AWLEAYH      D++   ++   L  RAG++QAA+
Sbjct: 171 -RGQIYWAKDIVFLVTEHDLLGTEAWLEAYH------DVNVTGMQSSPLQGRAGAIQAAV 223

Query: 235 NIEVQDLEIDHVDVRIEGLNGKLPNLDMFNLVQRIMAREGIASGYKQAPRKKRRHSQSHF 294
            +E+    +  +DV +EGLNG+LPNLD+ NL Q    + G+    +   + +   S    
Sbjct: 224 ALELSSDVVTSLDVAVEGLNGQLPNLDLLNLFQTFCQKGGLLCTLQGKLQPEDWTSLDGP 283

Query: 295 EQNFRQMLTMLASQSSGVPTGNHGLFHRYRIDALTIAANRRATHATLKGSPGSAAVPLLK 354
            Q  + +L M+  Q+SG P G+HGLF RYR++ALT+    R  ++  +      AV   K
Sbjct: 284 LQGLQTLLLMVLRQASGRPHGSHGLFLRYRVEALTL----RGINSFRQYKYDLVAV--GK 337

Query: 355 AIEGIARSLNNLLERFHQSFFFYVIVSNDRYISIGDYMPALVALVACAFLKAYLTWSTLP 414
           A+EG+ R LN+LLER HQSFF Y++    R++SIG YMPA+  L+    LKA   W  L 
Sbjct: 338 ALEGMFRKLNHLLERLHQSFFLYLLPGLSRFVSIGLYMPAVGFLLLVLGLKALELWMQLH 397

Query: 415 ATNAELEKAAGWLREHEQEAELEENLEPDKFELPYGSVLIYLTATLLI----GFLCNVLP 470
                LE+  G              L P +     G  L  L A LLI    G    VLP
Sbjct: 398 EAGMGLEEPGG-------APGPSVPLPPSQ-----GVGLASLVAPLLISQAMGLALYVLP 445

Query: 471 -LQQYFLEIPMGAAPLTTSVLSFLSL--IGFVLPF------VVVLPPGGLELLHVAFLLI 521
            L Q+        A     VL+ L++   G  LP           P  G   L +  L+ 
Sbjct: 446 VLGQHVATQHFPVAEAEAVVLTLLAIYAAGLALPHNTHRVVSTQAPDRGWMALKLVALIY 505

Query: 522 YGCALIVIGLLNFALGLFAAVLTVPLVIALETKEENSRSTLRNTIRLATLVMNPMMVVYV 581
               L  I L NF+LG   A   VP   A   K    R     T+  A LV+       +
Sbjct: 506 LALQLGCIALTNFSLGFLLATTMVPT--AALAKPHGPR-----TLYAALLVLTSPAATLL 558

Query: 582 IVLAMTFYQFPELPVQKIMLRAATAAMDASAYGLIDSVIYGNWLYFVICTIFLPLWIICW 641
             L + + +  E P+   +       + A A G+++   YG  L+ ++     P W++ W
Sbjct: 559 GSLFL-WRELQEAPLS--LAEGWQLFLAALAQGVLEHHTYGALLFPLLSLGLYPCWLLFW 615

Query: 642 TLSLSK 647
            +   K
Sbjct: 616 NVLFWK 621
>ref|NP_013189.1| (NC_001144) ER protein essential for attaching GPI
           (glycosylphosphatidylinositol) to protein; Gaa1p
           [Saccharomyces cerevisiae]
 sp|P39012|GAA1_YEAST GAA1 PROTEIN
 pir||S45053 membrane protein END2 - yeast (Saccharomyces cerevisiae)
 emb|CAA55944.1| (X79409) Gaa1 [Saccharomyces cerevisiae]
 gb|AAB67592.1| (U53880) Gaa1p [Saccharomyces cerevisiae]
 emb|CAA97649.1| (Z73260) ORF YLR088w [Saccharomyces cerevisiae]
          Length = 614

 Score =  570 bits (1455), Expect = e-161
 Identities = 143/682 (20%), Positives = 251/682 (35%), Gaps = 142/682 (20%)

Query: 15  LVDGLARHVRKVCYALYVAGVAWFFCLALPEFNHGTYLSENALSPGLVYPEIRIDANRLA 74
           LV  +   +  +     + G      L +      TY+SENAL P   Y   R ++    
Sbjct: 16  LVPRIIALLPVISMLCALFGFISIAILPMDGQYRRTYISENALMPSQAYSYFR-ESEWNI 74

Query: 75  IQLLEELQRERKDHLSTTPHAWIAAKMNEFGLETHTHNYTLRYPFGGGKEYHGKNIYGIL 134
           ++      +E  +  S   +  + + + EFG +T  +            E +G+ +YG++
Sbjct: 75  LRGYRSQIKEMVNMTSMERNNLMGSWLQEFGTKTAIYE----------NEQYGETLYGVM 124

Query: 135 RAPRIASTEGIVFAAPYRAASSVHTDISASVPLLLAFADFARRKNYWAKDLIFLITEQEQ 194
            APR   TE +V A P+  +        A   L ++ A F  R   W+K++I + +E  +
Sbjct: 125 HAPRGDGTEAMVLAVPWFNSDDEFNIGGA--ALGVSLARFFSRWPVWSKNIIVVFSENPR 182

Query: 195 LGMQAWLEAYHDGDRELDLSKAYLRPGNLPARAGSLQAALNIEVQDLE--IDHVDVRIEG 252
             +++W+EAYH     LDL+             GS++AA+ ++    E   ++V++  +G
Sbjct: 183 AALRSWVEAYHT---SLDLT------------GGSIEAAVVLDYSSTEDFFEYVEISYDG 227

Query: 253 LNGKLPNLDMFNLVQRIMAREGIASGYKQAPRKKRRHSQSHFEQNFRQMLTMLAS----- 307
           LNG+LPNLD+ N+   I   EG+       P      S      NF   L +L       
Sbjct: 228 LNGELPNLDLVNIAISITEHEGMKVSLHGLP------SDQLTNNNFWSRLKILCLGIRDW 281

Query: 308 QSSGV--PTGNHGLFHRYRIDALTIAANRRATHATLKGSPGSAAVPLLKAIEGIARSLNN 365
             SGV  P GN   F  +RI ++T+ A+         G+ G       +  E + RS+NN
Sbjct: 282 ALSGVKKPHGNEA-FSGWRIQSVTLKAH---------GNSGHDITTFGRIPEAMFRSINN 331

Query: 366 LLERFHQSFFFYVIVSNDRYISIGDYMPALVALVACAFLKAYLTWSTLPATNAELEKAAG 425
           LLE+FHQSFFFY++++  +++SI  Y+P+ VAL     + +   +      N  L     
Sbjct: 332 LLEKFHQSFFFYLLLAPRQFVSISSYLPSAVALSIAFAISSLNAFINNAYANISLFS--- 388

Query: 426 WLREHEQEAELEENLEPDKFELPYGSVLIYLTATLLIGFLCNVLPLQQYFLEIPMGAAPL 485
                            +   +      + L  + ++     ++P     + I M     
Sbjct: 389 -----------------EYNLVALLVWFVSLVISFVVSQAFLLIPSSGLLMTISM----- 426

Query: 486 TTSVLSFLSL---IGFVLPFVVVLPPGGLELLHVAFLLIYGCALIVIGLLNFALGLFAAV 542
             S    L L   I    P            L     L +      + ++NFA+ L    
Sbjct: 427 -ASCFLPLILSRKIHISEPLSYR--------LKNVAFLYFSLVSTSLLMINFAMALLIGT 477

Query: 543 LTVPLVI---------------------ALETK-------------EENSRSTLRNTIRL 568
           L  P+                         E K                 +   +    L
Sbjct: 478 LAFPMTFVKTIVESSSEHEVTTQSSNPIKTEPKDEIELVENHMDTTPATPQQQKQKLKNL 537

Query: 569 ATLVMNPMM--VVYVIVLAMTFYQFPELPVQKIMLRAATAAMDASAYGLIDSVIYGNWLY 626
             L++      +    +      +F    +   ++ A               +    W +
Sbjct: 538 VLLILTNPFISITLFGLFFDD--EFHGFDIINKLVSAW--------------LDLKCWSW 581

Query: 627 FVICTIFLPLWIICWTLSLSKR 648
           FV+C  +LP W++    S   +
Sbjct: 582 FVLCIGWLPCWLLILASSFESK 603
>pir||T46923 hypothetical protein DKFZp434E1512.1 - human
 emb|CAB75660.1| (AL157437) hypothetical protein [Homo sapiens]
 gb|AAH06383.1|AAH06383 (BC006383) Unknown (protein for MGC:12663) [Homo sapiens]
          Length = 499

 Score =  568 bits (1450), Expect = e-161
 Identities = 175/534 (32%), Positives = 253/534 (46%), Gaps = 51/534 (9%)

Query: 127 GKNIYGILRAPRIASTEGIVFAAPYRAASSVHTDISASVPLLLAFADFARRKNYWAKDLI 186
           G N+YGILRAPR ASTE +V   P  + S+    +   + L   F    R + YWAKD++
Sbjct: 4   GTNVYGILRAPRAASTESLVLTVPCGSDSTNSQAVGLLLALAAHF----RGQIYWAKDIV 59

Query: 187 FLITEQEQLGMQAWLEAYHDGDRELDLSKAYLRPGNLPARAGSLQAALNIEVQDLEIDHV 246
           FL+TE + LG +AWLEAYH      D++   ++   L  RAG++QAA+ +E+    +  +
Sbjct: 60  FLVTEHDLLGTEAWLEAYH------DVNVTGMQSSPLQGRAGAIQAAVALELSSDVVTSL 113

Query: 247 DVRIEGLNGKLPNLDMFNLVQRIMAREGIASGYKQAPRKKRRHSQSHFEQNFRQMLTMLA 306
           DV +EGLNG+LPNLD+ NL Q    + G+    +   + +   S     Q  + +L M+ 
Sbjct: 114 DVAVEGLNGQLPNLDLLNLFQTFCQKGGLLCTLQGKLQPEDWTSLDGPLQGLQTLLLMVL 173

Query: 307 SQSSGVPTGNHGLFHRYRIDALTIAANRRATHATLKGSPGSAAVPLLKAIEGIARSLNNL 366
            Q+SG P G+HGLF RYR++ALT+    R  ++  +      AV   KA+EG+ R LN+L
Sbjct: 174 RQASGRPHGSHGLFLRYRVEALTL----RGINSFRQYKYDLVAV--GKALEGMFRKLNHL 227

Query: 367 LERFHQSFFFYVIVSNDRYISIGDYMPALVALVACAFLKAYLTWSTLPATNAELEKAAGW 426
           LER HQSFF Y++    R++SIG YMPA+  L+    LKA   W  L      LE+  G 
Sbjct: 228 LERLHQSFFLYLLPGLSRFVSIGLYMPAVGFLLLVLGLKALELWMQLHEAGMGLEEPGG- 286

Query: 427 LREHEQEAELEENLEPDKFELPYGSVLIYLTATLLI----GFLCNVLP-LQQYFLEIPMG 481
                        L P +     G  L  L A LLI    G    VLP L Q+       
Sbjct: 287 ------APGPSVPLPPSQ-----GVGLASLVAPLLISQAMGLALYVLPVLGQHVATQHFP 335

Query: 482 AAPLTTSVLSFLSL--IGFVLPF------VVVLPPGGLELLHVAFLLIYGCALIVIGLLN 533
            A     VL+ L++   G  LP           P  G   L +  L+     L  I L N
Sbjct: 336 VAEAEAVVLTLLAIYAAGLALPHNTHRVVSTQAPDRGWMALKLVALIYLALQLGCIALTN 395

Query: 534 FALGLFAAVLTVPLVIALETKEENSRSTLRNTIRLATLVMNPMMVVYVIVLAMTFYQFPE 593
           F+LG   A   VP   A   K    R     T+  A LV+       +  L + + +  E
Sbjct: 396 FSLGFLLATTMVPT--AALAKPHGPR-----TLYAALLVLTSPAATLLGSLFL-WRELQE 447

Query: 594 LPVQKIMLRAATAAMDASAYGLIDSVIYGNWLYFVICTIFLPLWIICWTLSLSK 647
            P+   +       + A A G+++   YG  L+ ++     P W++ W +   K
Sbjct: 448 APLS--LAEGWQLFLAALAQGVLEHHTYGALLFPLLSLGLYPCWLLFWNVLFWK 499
>pir||T32771 hypothetical protein F33D11.9b - Caenorhabditis elegans
          Length = 885

 Score =  558 bits (1424), Expect = e-157
 Identities = 124/528 (23%), Positives = 214/528 (40%), Gaps = 80/528 (15%)

Query: 4   LSDPSISTQSKLVDGLARHVRKVCYALYVAG----VAWFFCLALPEFNHGTYLSENALSP 59
           +S    +T+ K++D + +  RKV   +   G      W F    P     T +SE++L P
Sbjct: 283 ISKDGGTTRPKILDKILK-FRKVALIIAYIGAYLSAQWLFD---PVNMEVTRISEHSLMP 338

Query: 60  GLVYPEIRIDANRLAIQLLEELQRERKDHLSTTPHAWIAAKMNEFGLETHTHNYTLRYPF 119
           GLV P  + D + +AIQL        K   S +    I    ++ GLE  TH +  +   
Sbjct: 339 GLVTP--KFDKSGIAIQLYRRFSDLPK---SKSQQELIHTIFSDLGLECFTHKWRSKV-- 391

Query: 120 GGGKEYHGKNIYGILRAPRIASTEGIVFAAPYRAASSVHTDISASVPLLLAFADFARRKN 179
             G   +G+N+YG +R PR    E  +       +      +S     +LAF D+A+ + 
Sbjct: 392 -AGNPMNGENVYGFIRGPRNDGAEAQMIVVQLGRSEKSRRMMSR----MLAFVDYAKDQV 446

Query: 180 YWAKDLIFLIT---------EQEQLGMQAWLEAYHDGDRELDLSKAYLRPGNLPARAGSL 230
           YWA+D++ +           E+    + A+L  Y   +         +    + A+ G+L
Sbjct: 447 YWARDIVIVFVDGGEKNDSIEKAAFALDAFLLKYQKIEALNSKKSITVEADEIQAQTGAL 506

Query: 231 QAALNIEVQDLEIDH---VDVRIEGLNGKLPNLDMFNLVQRIMAREGIASGYKQAPRKK- 286
              +  ++  + +     V+++  GLNG+  NLD+FN + +I          K   R   
Sbjct: 507 IGGVVYDLSGMAVKGQHIVNIQTNGLNGQQVNLDVFNGITKIA-------DSKHHSRVAI 559

Query: 287 ----RRHSQSHFEQN-FRQMLTMLASQSSGVPTGNHGLFHRYRIDALTIAANRRATHATL 341
                RHS  + E + F   +  L +Q+     G H +  +Y +  LT+      +H   
Sbjct: 560 FGTLHRHSNPYMEYSPFIVPMRALYTQAFISIEGIHSVLGKYGVQGLTVG----LSHDYS 615

Query: 342 KGSPGSAAVPLLKAIEGIARSLNNLLERFHQSFFFYVIVSNDRYISIGDYMPALVALVAC 401
           +           + IE ++R LNN+LER HQS+F YV+  +  + SI  Y+  L  LV+ 
Sbjct: 616 EKQT-------GQFIEEVSRMLNNVLERLHQSYFMYVLADDLHFSSIAMYIIPLTILVSP 668

Query: 402 AFLKAYLTWSTLPATNAELEKAAGWLREHEQEAELEENLEPDKFELPYGSVLIYLTATLL 461
             + AY  W+ +                  Q   +        F     +  ++ T    
Sbjct: 669 LVISAYFEWTKIQE---------------FQFPVVHLAFHVLGFFYYIATTYVFQT---- 709

Query: 462 IGFLCNVLPLQQYFLEI---PMGAAPLTTSVLSFLSLIGFVLPFVVVL 506
           IG    +  +Q  F+ I        P  + + SFL  I F++P    +
Sbjct: 710 IGTTFVLQWMQWPFVGIDGCDGQFTPSWSLINSFL--IFFMIPLGPYV 755
 Score =  158 bits (396), Expect = 3e-37
 Identities = 31/260 (11%), Positives = 63/260 (23%), Gaps = 108/260 (41%)

Query: 440 LEPDKFELPYGSVLIYLTATL--------------------------------------- 460
           L    F       L + +  +                                       
Sbjct: 637 LHQSYFMYVLADDLHFSSIAMYIIPLTILVSPLVISAYFEWTKIQEFQFPVVHLAFHVLG 696

Query: 461 ------------LIGFLCNVLPLQQYFLEI---PMGAAPLTTSVLSFLSLIGFVLPFVVV 505
                        IG    +  +Q  F+ I        P  + + SFL  I F++P    
Sbjct: 697 FFYYIATTYVFQTIGTTFVLQWMQWPFVGIDGCDGQFTPSWSLINSFL--IFFMIPLGPY 754

Query: 506 LPPGGLELLHVAFLLIYGCALIVIGLLNFALGLFAAVLTVPLVIALETKEENSRSTLRNT 565
           +                                                    RS  R++
Sbjct: 755 V-------------------------------FLKT-----------------RSIFRSS 766

Query: 566 IRLATLVMNPMMVVYVIVLAMTFYQFPELPVQKIMLRAATAAMDASAYGLIDSVIYGNWL 625
           +      ++ +  V + +  M    FP +     +  A + A+ A    +   V +    
Sbjct: 767 VLAILNPISVLAFVAINIPGMFGQLFPTISQDSWLAAAQSIALTAIREDVEFKVNH---- 822

Query: 626 YFVICTIFLPLWIICWTLSL 645
           +F++C    P+W + +  S+
Sbjct: 823 FFLLCCFGYPIWNMLFACSM 842
>emb|CAB65611.1| (AL136078) putative glycosylphosphatidylinositol anchor attachment
           protein [Schizosaccharomyces pombe]
          Length = 581

 Score =  534 bits (1361), Expect = e-150
 Identities = 148/578 (25%), Positives = 242/578 (41%), Gaps = 77/578 (13%)

Query: 12  QSKLVDGLARHVRKVCYALYVAGVAWFFCLALPEFNHGTYLSENALSPGLVYPEIRIDAN 71
           Q ++   L RH+  +  +L + G++W F L   E     ++SE+AL PG V        N
Sbjct: 8   QIRVFPFLQRHLFFLQLSLTLIGLSWIFILPRNEIIDRLHVSESALLPGQVNTYFE---N 64

Query: 72  RLAIQLLEELQRERK-DHL-----STTPHAWIAAKMNEFGLETHTHNYTLRYPFGGGKEY 125
           R +  +   L       HL     + T +  +       GL T   NY++  P   G E+
Sbjct: 65  RYSKTVSSSLTAANTWSHLDASVGTNTMYDDLEQIFTAMGLPTQKQNYSINIP---GSEF 121

Query: 126 HGKNIYGILRAPRIASTEGIVFAAPYRAASSVHTDISASVPLLLAFADFARRKNYWAKDL 185
           +G N    LRAPR  +TE ++   P++     + +  A V L ++   + +  + W+KD+
Sbjct: 122 NGSNFITTLRAPRGDATESLLLCVPWKDHIGQYNE--AGVALAISLLKYFQGWSLWSKDI 179

Query: 186 IFLITEQEQLGMQAWLEAYHDGDRELDLSKAYLRPGNLPARAGSLQAALNIEVQDLEI-- 243
           I +I +    G  ++L +Y       D +  Y+    L  R+GS+QA L++E+   E   
Sbjct: 180 ILVIFDDPVYGPSSFLTSY------FDQTTPYISYTPLKIRSGSIQAGLSLELVTTENNS 233

Query: 244 DHVDVRIEGLNGKLPNLDMFNLVQRIMAREGIASGYKQAPRKKRRHSQSHFEQNFRQMLT 303
           D ++V  +  NG+LPNLD+FN + RI  +       +        +S S +    + +  
Sbjct: 234 DVLEVLYQATNGQLPNLDLFNTISRIFMQH-FNYPLRLQGYDFHANSGSSYTSRLKSLWM 292

Query: 304 MLASQSSGVPTGNHGLFHRYRIDALTIAANRRATHATLKGSPGSAAVPLLKAIEGIARSL 363
            + +Q+    T  H LF +YRID LT+          +K           +AIE   RSL
Sbjct: 293 GMLTQAVSNVTSAHALFPQYRIDMLTL-------RMKVKDPFSFDMFRFGQAIESTFRSL 345

Query: 364 NNLLERFHQSFFFYVIVSNDRYISIGDYMPALVALVACAFLKAYLTWSTLPATNAELEKA 423
           NNLLE  HQSFFFY I+ +  +ISIG+YMP+++ L A   L AY  W        +L + 
Sbjct: 346 NNLLEHLHQSFFFYFILDHLHFISIGNYMPSILILAASFMLGAYRHWIN-HEKKIDLWRP 404

Query: 424 AG-WLREHEQEAELEENLEPDKFELPYGSVLIYLTATLLIGFLCNVLPLQQYFLEIPMGA 482
              WL                 F       L+  +  + +     ++             
Sbjct: 405 FSFWLF--------------SIFCTIAAYYLVSSSTKITVFIFLYLM------------- 437

Query: 483 APLTTSVLSFLSLIGFVLPFVVVLPPGGLELLHVAFLLIYGCALIVIGLLNFALGLFAAV 542
                  L+F+ +I     F   +     EL+    L+     + V+  LNF+L    A+
Sbjct: 438 -------LTFIGII-----FSTFMTSEDAELVLSYDLMSKSLFISVVSTLNFSLSFVVAI 485

Query: 543 LTVPLVIALETKEENSRSTLRNTIRLATLVMNPMMVVY 580
           L VPL         + R   R ++  A L      +  
Sbjct: 486 LLVPLQFI------SFRFNRRLSLLFAVLTYFSTFIFL 517
>ref|NP_197414.1| (NM_121918) GPAA1 - like protein [Arabidopsis thaliana]
          Length = 696

 Score =  310 bits (787), Expect = 6e-83
 Identities = 99/373 (26%), Positives = 154/373 (40%), Gaps = 65/373 (17%)

Query: 294 FEQNFRQMLTMLASQSSGVPTGNHGLFHRYRIDALTIAANRRATHATLKGSPGSAAVPLL 353
           + +    + + L SQ+ G+PTG HG F  Y++DA+T+  + R      K       +   
Sbjct: 357 YLEGSATLASSLYSQALGIPTGPHGAFRDYQVDAITLKVSPRFPPD-SKTRQHDFFLRGA 415

Query: 354 KAIEGIARSLNNLLERFHQSFFFYVIVSNDRYISIGDYMPALVALVACAFLKA------- 406
           + +EG  RS+NNLLE+FHQSFF Y++ S  ++IS+G YM A   LVA   + A       
Sbjct: 416 RLLEGTIRSVNNLLEKFHQSFFLYMLTSPSKFISVGVYMIAFALLVAPLPMVAASLYIDG 475

Query: 407 --YLTWSTLPATNAELEKAAGWLREHEQEAELEENLEPDKFELPYGSVLIYLTATLLIGF 464
              LT ST   T  E  K+  WL   +Q   L                        L+GF
Sbjct: 476 CKSLTNSTHNPT--ENFKSWKWLDAAKQVFTLH-----------------------LLGF 510

Query: 465 LCNVLPLQQYFL-EIPMGAAPLTTSVL-----SFLSLIGFVL-----PFVVVLPPGGLEL 513
           +  +LP   YF+ ++P   +P   S++     S L LI FV      PF   L      +
Sbjct: 511 IVTLLP---YFICQVPGQHSPTNRSIMWGTTSSSLLLITFVTMPGCSPFSSRLHGTNWAI 567

Query: 514 LHVAFLLIYGCALIVIGLLNFALGLFAAVLTVPLVIALETKEENSRSTLRNTIRLA---- 569
           L    +      L ++ ++NFA     A+L VP  +  +  +   RS    ++  A    
Sbjct: 568 LKSVTISAAFIGLCLMSIINFATAEIGALLLVPTCLMAQPIKPALRSRRLKSLLGAFCSI 627

Query: 570 -TLVMNPMMVVYVIVLAMTFYQFPELPVQKIMLRAATAAMDASAYGLIDSVIYGNWLYFV 628
             L +   ++ + I   +       L    +     T      A+    S  Y   LY  
Sbjct: 628 VLLTIGFPVMFFAISKGLLGE---GLVGLSLGGEFWTWLESLWAW---KSATY---LY-- 676

Query: 629 ICTIFLPLWIICW 641
           I  + LP W++C 
Sbjct: 677 IGMVHLPCWLLCL 689
 Score =  287 bits (727), Expect = 5e-76
 Identities = 69/293 (23%), Positives = 121/293 (40%), Gaps = 27/293 (9%)

Query: 7   PSISTQSKLVDGLARHVRKVCYALYVAGVAWFFCLALPEFNHGTYLSENALSPGLVYPEI 66
           P I  +  +  G+        +++  +       L LP     TY+SENAL PG     +
Sbjct: 19  PKIKPRPIVRLGIFLIAHSPVFSVVFSAAGVLALLLLPLLAKNTYISENALMPGSARSML 78

Query: 67  RIDANRLAIQLLEELQRERKDHLSTTPHAWIAAKMNEFGLETHTHNYTLR-------YPF 119
                    +L+++++  R +H        I   M++ G E     +          + F
Sbjct: 79  SNRDVSDGSKLVKDIKNFRLNHEGQV-QKLIGKYMSDMGAEVSYQKFHPEGNQFHPLHFF 137

Query: 120 GGGKEY----------HGKNIYGILRAPRIASTEGIVFAAPYRAASSVHTDISASVPLLL 169
            G   Y          +G N+ GI+RAPR    E IV   PY   +    +   S+ ++ 
Sbjct: 138 SGPDSYTLLENVSCASYGVNVAGIIRAPRGDGKESIVLVTPYDFINGGDYE-DLSLGIVS 196

Query: 170 AFADFARRKNYWAKDLIFLITEQ---EQLGMQAWLEAYHDGDREL-DLSKA-YLRPGNLP 224
           +      R  + +KD+I+L+ +    +   + AWL  YH    ++ DL K       +  
Sbjct: 197 SLFSLLSRVTWLSKDIIWLVADSRYGDYRPVAAWLTEYHSPSFKVSDLLKCDEQNTADNF 256

Query: 225 ARAGSLQAALNIEV---QDLEIDHVDVRIEGLNGKLPNLDMFNLVQRIMAREG 274
            RAG++ AAL ++V    +   D + +  E  NG++PNLD+ N+V  +     
Sbjct: 257 RRAGTMAAALVLKVDGRSEKFEDTLSIYAEASNGQMPNLDLINVVNYLAVHRQ 309
>emb|CAB86709.1| (AL163505) hypothetical protein L3640.14 [Leishmania major]
          Length = 464

 Score =  184 bits (464), Expect = 4e-45
 Identities = 36/157 (22%), Positives = 65/157 (40%), Gaps = 7/157 (4%)

Query: 232 AALNIEVQDLEIDHVDVRIEGLNGKLPNLDMFNLVQRIMAREGIASGYKQAPRKKRRHSQ 291
           AAL + +  L   H+ +   G NG  PN D  N+  ++ A       Y    +++ R + 
Sbjct: 137 AALVLNLSTLYNRHICIDTLGGNGIQPNQDYTNIAVQLAAANQFLPSY--LCQRRHRSTD 194

Query: 292 SHFEQNFRQMLTML--ASQSSGVPTGNHGLFHRYRIDALTIAANRRATHATLKGSPGSAA 349
                 F      L  + Q    P   H +   + I+ + I+++   + +++        
Sbjct: 195 DA-HAGFWHYWAYLEASVQLPTRPQPWH-IIPTHSINTIAISSDPLYSASSV-FPHSQLP 251

Query: 350 VPLLKAIEGIARSLNNLLERFHQSFFFYVIVSNDRYI 386
               + +  I  SLN L E+FH S F ++ VS  RY+
Sbjct: 252 DAFAQLVLKIIVSLNGLEEKFHHSTFVWLPVSLWRYV 288
>ref|NP_491701.1| (NM_059300) F33D11.9a.p [Caenorhabditis elegans]
 pir||T32772 hypothetical protein F33D11.9a - Caenorhabditis elegans
 gb|AAB96702.1| (AF039720) Hypothetical protein F33D11.9a [Caenorhabditis elegans]
          Length = 229

 Score =  141 bits (354), Expect = 3e-32
 Identities = 22/116 (18%), Positives = 45/116 (37%), Gaps = 11/116 (9%)

Query: 530 GLLNFALGLFAAVLTVPLVIALETKEENSRSTLRNTIRLATLVMNPMMVVYVIVLAMTFY 589
            L+N  L  F  +   P V          RS  R+++      ++ +  V + +  M   
Sbjct: 82  SLINSFLIFFM-IPLGPYVFLKT------RSIFRSSVLAILNPISVLAFVAINIPGMFGQ 134

Query: 590 QFPELPVQKIMLRAATAAMDASAYGLIDSVIYGNWLYFVICTIFLPLWIICWTLSL 645
            FP +     +  A + A+ A    +   V +    +F++C    P+W + +  S+
Sbjct: 135 LFPTISQDSWLAAAQSIALTAIREDVEFKVNH----FFLLCCFGYPIWNMLFACSM 186
 Score =  113 bits (282), Expect = 8e-24
 Identities = 22/120 (18%), Positives = 39/120 (32%), Gaps = 24/120 (20%)

Query: 390 DYMPALVALVACAFLKAYLTWSTLPATNAELEKAAGWLREHEQEAELEENLEPDKFELPY 449
            Y+  L  LV+   + AY  W+ +                  Q   +        F    
Sbjct: 1   MYIIPLTILVSPLVISAYFEWTKIQE---------------FQFPVVHLAFHVLGFFYYI 45

Query: 450 GSVLIYLTATLLIGFLCNVLPLQQYFLEI---PMGAAPLTTSVLSFLSLIGFVLPFVVVL 506
            +  ++ T    IG    +  +Q  F+ I        P  + + SFL  I F++P    +
Sbjct: 46  ATTYVFQT----IGTTFVLQWMQWPFVGIDGCDGQFTPSWSLINSFL--IFFMIPLGPYV 99
>ref|NP_375951.1| (NC_003106) 815aa long hypothetical quinol oxidase polypeptide
           I/III [Sulfolobus tokodaii]
 dbj|BAB65060.1| (AP000981) 815aa long hypothetical quinol oxidase polypeptide I/III
           [Sulfolobus tokodaii]
          Length = 815

 Score = 35.0 bits (80), Expect = 3.6
 Identities = 39/240 (16%), Positives = 76/240 (31%), Gaps = 20/240 (8%)

Query: 375 FFYVIVSNDRYISIGDYMPALVALVACAFLKAYLTWSTLPATNAELEKAAGWLREHEQEA 434
           + Y  +S D  ++ G  M  +   +  A + + LT      T   L+K   +       A
Sbjct: 134 YMYAPLSVDLQVNSGIGMDVIEIALIIAGISSTLTGINFILTITRLKKIPYFKMPLFVWA 193

Query: 435 ELEENLEPDKFELPYGSVLIYLTATLLIGFLCNVL-----PLQQYFLEIPMGAAPLTTSV 489
                +       P  + L         GF          PL    +    G   +   +
Sbjct: 194 FFSTAILLMIALPPLTAGLAMAFLERNWGFQFFNANAGGNPLLWQNVFWFFGHPEVYILI 253

Query: 490 LSFLSLIGFVLPFVVVLPPGGLELLHVAFLLIYGCALIVI-------GLLNF-----ALG 537
           L  + +IG VLP  V     G + L ++   I   + + +        + NF     +  
Sbjct: 254 LPAMGIIGEVLPRSVGRQIYGYKALALSSFAIAFLSAMGVWMHHMFTSIFNFWADAVSSA 313

Query: 538 LFAAVLTVPLVIALETKEENSRSTLRNTIRLATLVMNPMMVVYVIVLAMTFYQFPELPVQ 597
           +  A+  +P  + +                 + L +  + +  V    +T   FP +P+ 
Sbjct: 314 ITMAI-AIPSGVKVVNWTATFYQGKLRVTGYSLLTIGFIALFLVG--GITGVLFPLIPLD 370
>ref|NP_391656.1| (NC_000964) amino acid permease [Bacillus subtilis]
 sp|P39636|ROCC_BACSU Amino-acid permease rocC
 pir||S39733 amino acid permease rocC - Bacillus subtilis
 emb|CAA51634.1| (X73124) ipa-78d [Bacillus subtilis]
 emb|CAB15803.1| (Z99123) amino acid permease [Bacillus subtilis]
          Length = 470

 Score = 34.2 bits (78), Expect = 7.7
 Identities = 36/261 (13%), Positives = 70/261 (26%), Gaps = 35/261 (13%)

Query: 390 DYMPALVALVAC-AFLKAYLTWSTLPATN-AELEKAAGWLREHEQ--EAELEENLEPDKF 445
            +M AL  ++    FL +  T S              G+L         EL   +     
Sbjct: 17  LFMIALGGVIGTGLFLGSGFTISQAGPLGAIAAYIIGGFLMYLVMLCLGELAVAMPVAGS 76

Query: 446 ELPYGSVLIYLTATLLIGFLCNVLP---------LQQYFLEIPMGAAPLTTSVLSFLSLI 496
              Y +  +  +   +IG+L                   ++  + + P+    L F  +I
Sbjct: 77  FQAYATKFLGQSTGFMIGWLYWFSWANTVGLELTSAGILMQRWLPSVPIWIWCLVFGIVI 136

Query: 497 GFVLPFVVV--LPPGGLELLHVAFLLIYGCALIVIGLLNFALGLFAAVLTVPLVIALETK 554
             +    V                 +I    +    +  F L  F      P      + 
Sbjct: 137 FLINALSVRSFAEMEFWFSSIKVAAIILFIVIGGAAV--FGLIDFKGGQETPF----LSN 190

Query: 555 EENSRSTLRNTIRLATLVMNPMMVVYVIVLAMT-FYQFPELPVQKIMLRAATAAMDASAY 613
               R    N +          M   V+V       +   +          + + + +  
Sbjct: 191 FMTDRGLFPNGVLAV-------MFTLVMVNFSFQGTELVGI------AAGESESPEKTLP 237

Query: 614 GLIDSVIYGNWLYFVICTIFL 634
             I +VI+    +FV+    L
Sbjct: 238 KSIRNVIWRTLFFFVLAMFVL 258
CPU time:   125.80 user secs.	    2.44 sys. secs	  128.24 total secs.

  Database: nr
    Posted date:  Apr 21, 2002  2:19 PM
  Number of letters in database: 277,845,442
  Number of sequences in database:  887,402
  
Lambda     K      H
   0.315    0.163    0.443 

Gapped
Lambda     K      H
   0.270   0.0572    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 325573997
Number of Sequences: 887402
Number of extensions: 12446188
Number of successful extensions: 48523
Number of sequences better than 10.0: 440
Number of HSP's better than 10.0 without gapping: 19
Number of HSP's successfully gapped in prelim test: 421
Number of HSP's that attempted gapping in prelim test: 48261
Number of HSP's gapped (non-prelim): 555
length of query: 674
length of database: 277,845,442
effective HSP length: 57
effective length of query: 617
effective length of database: 227,263,528
effective search space: 140221596776
effective search space used: 140221596776
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.3 bits)
S2: 76 (33.7 bits)