Glycogen- and PP1C-binding motifs of PTG (Protein Targeting to Glycogen) and other protein phosphatases

The Amylase Connection

Peer Bork
Thomas Dandekar
Frank Eisenhaber
Martijn Huynen


Step 1 : BLAST-searches with PTG and closely related sequences
**************************************************************

BLAST-search with PTG
 
/:gpnew|U89924|1888566 protein phosphatase 1 binding prot...  1506  1.2e-154  1
/:trembl|S80360|RS3605_1 product: "hepatic glycogen-bindi...   568  3.0e-55   1
/:trembl|U88313|CEUF15G10_2 gene: "F15G10.1";  Caenorhabd...   211  4.2e-21   2
/:trembl|X78578|HSPPPAGB_1 gene: "PPP1R3"; product: "prot...   217  1.2e-16   1
/:trembl|M65109|OSRG1A_1 gene: "RG1"; product: "protein p...   212  3.9e-16   1
/:swissnew|P40036|GIP2_YEAST GLC7-INTERACTING PROTEIN 2./...   186  1.3e-13   2
/:swissnew|P40187|PIG2_YEAST GSY2 INTERACTING PROTEIN PIG...   172  1.2e-10   1
/:swissnew|P28006|GAC1_YEAST PROTEIN PHOSPHATASE 1 REGULA...   155  2.1e-08   1
/:swiss|P28006|GAC1_YEAST GAC1 PROTEIN.//:trembl|X63941|S...   155  2.1e-08   1
/:swissnew|Q06216|PIG1_YEAST PROTEIN PHOSPHATASE 1 REGULA...    96  0.00013   2
/:swiss|P07683|AMYG_RHIOR GLUCOAMYLASE PRECURSOR (EC 3.2....   104  0.0085    1

Reciprocal BLAST-searches with the animal (PTG, rGl, cGl, hGl, RG1) and yeast
(GIP2, PIG2, GAC1, PIG1) phosphatases return the same family.

AMYG_RHIOR is a modular protein. The first 150 amino acid have a similarity
to a domain in the phosphatases. Then, the catalytic domain follows in the 
sequence.

A reciprocal BLAST-search with the first 200 amino acids of AMYG_RHIOR 
(a fungal glycoamylase of family 15) returns phosphatases (including
PTG with P=2.e-8 !! and also GAC1, PIG2, RG1, and GIP2 in the twilight zone) 
as well as two other amylases:

/:pironly|JC4510|JC4510 alpha-amylase (EC 3.2.1.1) precur...   126  2.8e-07   1
     --> Lipomyces kononenkoae (yeast)
/:swiss|P42042|AMYG_ARXAD GLUCOAMYLASE PRECURSOR (EC 3.2....    96  4.1e-06   2
     --> ARXULA ADENINIVORANS.
         EUKARYOTA; FUNGI; DEUTEROMYCOTINA (IMPERFECT FUNGI).
         SIMILARITY: BELONGS TO FAMILY 15 OF GLYCOSYL HYDROLASES.
/:gpnew|U89924|1888566 protein phosphatase 1 binding prot...   103  5.2e-05   1
/:swissnew|P28006|GAC1_YEAST PROTEIN PHOSPHATASE 1 REGULA...    76  0.00023   3
/:swiss|P28006|GAC1_YEAST GAC1 PROTEIN.//:trembl|X63941|S...    76  0.00023   3
/:swissnew|P40187|PIG2_YEAST GSY2 INTERACTING PROTEIN PIG...   100  0.00085   1
/:trembl|M65109|OSRG1A_1 gene: "RG1"; product: "protein p...    99  0.0031    1
/:swissnew|P40036|GIP2_YEAST GLC7-INTERACTING PROTEIN 2./...    91  0.024     1
/:trembl|S80360|RS3605_1 product: "hepatic glycogen-bindi...    87  0.033     1


Regions of sequence similarity as given in BLAST alignments in the 
BLAST output of the PTG search (sequence positions as in the original 
sequences):

PTG  33-293  rGl         31-283
    131-237  cGl        173-280
     60-234  hGl         61-229
     33-241  RG1         36-239
    144-233  GIP2       437-533
    146-233  PIG2       408-507
    149-249  GAC1       258-375
    120-199  PIG1       206-275
    134-233  AMYG_RHIOR  34-126

I.e., the N-terminus of the domain studied is defined by the sequence of
AMYG_RHIOR. The C-terminus is confined by some short phosphatases (PTG, cGl).

Regions of sequence similarity as given in BLAST alignments in the 
BLAST output of the AMYG_RHIOR search (sequence positions as in the original 
sequences):

AMYG_RHIOR  35-127  JC4510       49-130
            36-129  AMYG_ARXAD   36-131



Step 2 : Extraction of sequence motifs for MOST-searches
********************************************************
We produced a multiple alignment of the 10 sequences (9 phosphatases and
AMYG_RHIOR) with clustalw. Two most significant motifs could be extracted.

motif 1
*******
VFVKNIYFDK
ILVKNIFYDK
VYVKNLSFEK
IFVNNLNFEK
IRVLNISFEK
IRVLNVSFEK
VKVKNVSFEK
VKVQNLAFEK
IKVGNIAFEK
IYVKNIAYSK

results of the MOST-search:
--> only hits with low significance (E>0.242 and R>0.019)
VC21_VACCC
CEF54F7    (KIN, tRNA)
SSD902     (only one of the proteins)
AMYG_ARXAD (was already found --> reciprocal BLAST with AMYG_RHIOR)

MOST output:
                                        ....:....|.  Score  Expected   Ratio
  >/:swiss|P21105|VC21_             97: VFmKNISFDK   168,     0.242    0.0192
  >/:tremblnew|Z67755|C            300: VFVNNLdFDK   165,     0.334    0.0263
  >/:trembl|D90902|SSD9            465: VKVLNIYFgK   161,     0.491    0.0409
  >/:swiss|P42042|AMYG_             50: aFVKNLSYDK   159,     0.579    0.0494
  >/:swissnew|P28006|GA   !   3    262: VYVKNLSFEK   207,  0.000366  0.000177
  >/:swissnew|P40187|PI   !   1    412: VFVKNIYFDK   200,   0.00169  0.000548
  >/:tremblnew|U89924|M   !   6    150: VKVKNVSFEK   198,   0.00241  0.000586
  >/:trembl|U88313|CEUF   !   8    194: IKVGNIAFEK   192,   0.00717    0.0014
  >/:trembl|S80360|RS36   !   7    148: VKVQNLAFEK   191,   0.00854   0.00139
  >/:swissnew|Q06216|PI   !   4    228: IFVNNLNFEK   188,    0.0143   0.00175
  >/:swiss|P07683|AMYG_   !   9     50: IYVKNIAYSK   188,    0.0143   0.00175
  >/:trembl|M65109|OSRG   !   5    148: IRVLNISFEK   183,    0.0329    0.0036
  >/:swissnew|P40036|GI   !   2    443: ILVKNIFYDK   177,    0.0788   0.00796



motif 2
*******
EYWDNNDSANY
EYWDNNNGNNY
TYYDNNNGKNY
SFYDNNDYKNY
TFWSNNNGTNY
TFWSNNNGTNY
IFWDNNEGQNY
SYWDSNKGKNY
EYWDSNSGTNY
TYYDNNNSANY

results of the MOST-search:
two new glycohydrolases
1)  AMY_CLOAB|P23671 CLOSTRIDIUM ACETOBUTYLICUM
    SIMILARITY: BELONGS TO FAMILY 13 OF GLYCOSYL HYDROLASES
MOST output:
                                        ....:....|.  Score  Expected   Ratio
  >/:swiss|P23671|AMY_C            452: gtWDNNNSNNY   196,     0.019   0.00155
  >/:swissnew|P40036|GI   !   2    521: EYWDNNNGNNY   264,  7.43e-07  7.19e-07
  >/:swissnew|P28006|GA   !   3    347: TYYDNNNGKNY   256,  3.97e-06  1.28e-06
  >/:swissnew|P40187|PI   !   1    495: EYWDNNDSANY   247,  2.17e-05  5.25e-06
  >/:trembl|S80360|RS36   !   7    219: SYWDSNKGKNY   240,   6.9e-05  1.33e-05
  >/:trembl|M65109|OSRG   !   5    219: TFWSNNNGTNY   239,  8.17e-05  7.89e-06
  >/:gpnew|U89924|18885   !   6    221: IFWDNNEGQNY   239,  8.17e-05  7.89e-06
  >/:trembl|U88313|CEUF   !   8    265: EYWDSNSGTNY   239,  8.17e-05  7.89e-06
  >/:swiss|P07683|AMYG_   !   9    117: TYYDNNNSANY   239,  8.17e-05  7.89e-06
  >/:swissnew|Q06216|PI   !   4    318: SFYDNNDYKNY   227,  0.000447  3.93e-05

2)  SLAMLC_2|Z86113;
    "amlC"; S.lividans amlC gene
MOST output:
                                        ....:....|.  Score  Expected   Ratio
  >/:tremblnew|Z86113|S             86: GTWDNNgGeNY   202,    0.0101  0.000755
  >/:swissnew|P40036|GI   !   2    521: EYWDNNNGNNY   270,  1.68e-07  1.62e-07
  >/:swissnew|P28006|GA   !   3    347: TYYDNNNGKNY   257,   4.8e-06  1.55e-06
  >/:swissnew|P40187|PI   !   4    495: EYWDNNDSANY   250,  1.81e-05  4.37e-06
  >/:swiss|P07683|AMYG_   !   9    117: TYYDNNNSANY   242,  6.71e-05   1.3e-05
  >/:trembl|S80360|RS36   !   5    219: SYWDSNKGKNY   239,  0.000103  1.67e-05
  >/:trembl|M65109|OSRG   !   6    219: TFWSNNNGTNY   238,  0.000119  1.43e-05
  >/:gpnew|U89924|18885   !   7    221: IFWDNNEGQNY   237,  0.000137  1.48e-05
  >/:trembl|U88313|CEUF   !   8    265: EYWDSNSGTNY   236,  0.000159  1.53e-05
  >/:swiss|P23671|AMY_C   !   1    452: GTWDNNNSNNY   230,  0.000358  3.15e-05
  >/:swissnew|Q06216|PI   !  10    318: SFYDNNDYKNY   227,  0.000528  4.26e-05

There are internal repeats in AMY_CLOAB (3 repeats) and in SLAMLC_2 (2 repeats).
Independent BLAST searches were carried out with each repeat.

results of reciprocal BLAST-searches with SLAMLC_2 (first 210 amino acids):
many new amylases
/:trembl|Y00150|BPAMYB_1 product: "precursor polypeptide"...   371  1.9e-33   1
/:swiss|P21543|AMYB_BACPO BETA-AMYLASE (EC 3.2.1.2) / ALP...   368  9.9e-33   1
/:trembl|X53373|UCDSMAMY_1 product: "precursor polypeptid...   305  8.5e-26   1
/:swiss|P06547|AMYB_BACCI BETA-AMYLASE PRECURSOR (EC 3.2....   184  1.5e-13   1
/:trembl|X55452|BSG6AMA_1 gene: "G6-amylase";  Bacillus s...   181  6.6e-13   1
/:swiss|P23671|AMY_CLOAB PUTATIVE ALPHA-AMYLASE (EC 3.2.1...   137  1.2e-08   1

There are internal repeats in AMYB_BACPO and (less pronounced) also in 
UCDSMAMY_1.


Step 3 : Extraction of sequence profiles for SEARCHWISE-searches
****************************************************************
production of a multiple alignment of the amylases (including their repeats)
found in step 2 with clustalw and extraction of a pattern for the alignment
part including motifs 1 and 2

SEARCHWISE-output (BLOSUM62):
  1 19470    535  :swiss|P21543|AMYB_BACPO BETA-AMYLASE (EC 3.2.1.2) / ALPHA-AMYLASE (EC
  2 19340    648  :trembl|Y00150|BPAMYB_1 product: "precursor polypeptide";  B.polymyxa 
  3 18560    538  :swiss|P06547|AMYB_BACCI BETA-AMYLASE PRECURSOR (EC 3.2.1.2) (1,4-ALPH
  4 18230   1054  :trembl|X53373|UCDSMAMY_1 product: "precursor polypeptide (AA -37 to 1
  5 17600    206  :tremblnew|Z86113|SLAMLC_2 gene: "amlC";  S.lividans amlC gene //:gpne
  6 17180    940  :trembl|X55452|BSG6AMA_1 gene: "G6-amylase";  Bacillus sp. H-167 G6-am
  7 13146    371  :swiss|P23671|AMY_CLOAB PUTATIVE ALPHA-AMYLASE (EC 3.2.1.1) (1,4-ALPHA

--> new findings
  8  4748    887  :swiss|P30269|AMY_BUTFI ALPHA-AMYLASE PRECURSOR (EC 3.2.1.1) (1,4-ALPH
  9  4660    229  :trembl|S80360|RS3605_1 product: "hepatic glycogen-binding subunit pro
 10  4568    427  :trembl|X95759|STSS_1 product: "glycogen (starch) synthase";  S.tubero
 11  4568    427  :tremblnew|X94400|STSSSIII_1 gene: "SSSIII"; product: "soluble-starch-
 12  4500    278  :trembl|U88313|CEUF15G10_2 gene: "F15G10.1";  Caenorhabditis elegans c
 13  4460    231  :tremblnew|U89924|MMU89924_1 product: "protein phosphatase 1 binding p
 14  4450    531  :swissnew|P40036|GIP2_YEAST GLC7-INTERACTING PROTEIN 2.//:swiss|P40036

SEARCHWISE-output (GON250):
  1 19940    535  :swiss|P21543|AMYB_BACPO BETA-AMYLASE (EC 3.2.1.2) / ALPHA-AMYLASE (EC
  2 19840    648  :trembl|Y00150|BPAMYB_1 product: "precursor polypeptide";  B.polymyxa
  3 19610   1054  :trembl|X53373|UCDSMAMY_1 product: "precursor polypeptide (AA -37 to 1
  4 18980    538  :swiss|P06547|AMYB_BACCI BETA-AMYLASE PRECURSOR (EC 3.2.1.2) (1,4-ALPH
  5 18800    208  :tremblnew|Z86113|SLAMLC_2 gene: "amlC";  S.lividans amlC gene //:gpne
  6 18510    940  :trembl|X55452|BSG6AMA_1 gene: "G6-amylase"; Bacillus sp. H-167 G6-am
  7 14148    466  :swiss|P23671|AMY_CLOAB PUTATIVE ALPHA-AMYLASE (EC 3.2.1.1) (1,4-ALPHA

--> new findings
  8  7324    887  :swiss|P30269|AMY_BUTFI ALPHA-AMYLASE PRECURSOR (EC 3.2.1.1) (1,4-ALPH
  9  6754    599  :trembl|U22045|BS22045_1 gene: "Amy"; product : "alpha-amylase";  Bacil
--> less likely new findings
 10  6251    612  :swiss|P14804|AMYG_NEUCR GLUCOAMYLASE PRECURSOR (EC 3.2.1.3) (GLUCAN 1
 11  6044    555  :trembl|D49448|CRGG2A_1 product: "glucoamylase G2";  Corticium rolfsii
 12  5601    372  :pdb|1INV|1INV Influenza bLEE40 neuraminidase (sialidase) complexed wi


The starch synthetases STSS_1 and STSSSIII_1 contain internal repeats.
Reciprocal BLAST-searches with the starch synthetases as well as with AMY_BUTFI
do not give new hits in the region of the profile.



Step 4 : BLAST-searches with the sequence of BS22045_1
******************************************************
region of similarity with the profile amyg-2w-gon250
                 * *       *       *   *  * * *           *   *       *
amyg-2w-go    41 GGWTAVPGVAMvSAEDGGWa.KITVPIGsASQLNAAFNNGSGt..WDNNNGNNYN
BS22045_1    542 GNWNTANAIKMNPSSYPTWKATIALPQGKAIEFKFIKKDQAGNVIWESTSNRTYT

amyg-2w-go    93 FS
BS22045_1    597 VP

BLAST-results with BS22045_1 (last part of the sequence)
/:trembl|U22045|BS22045_1 gene: "Amy"; product: "alpha-am...   630  8.1e-62   1
/:pdb|1CYG|1CYG Cyclodextrin glucanotransferase (cgtase)       249  2.1e-21   2
/:swiss|P31797|CDGT_BACST CYCLOMALTODEXTRIN GLUCANOTRANSF...   249  2.5e-21   2
/:swiss|P31746|CDGT_BACS2 CYCLOMALTODEXTRIN GLUCANOTRANSF...   220  2.0e-20   2
/:trembl|D13068|BSCYGT_1 product: "cyclodextrin glucanotr...   220  2.2e-20   2
/:swiss|P22998|AMY_STRVL ALPHA-AMYLASE PRECURSOR (EC 3.2....   245  3.6e-20   1
/:swiss|P19584|AMYB_THETU BETA-AMYLASE, THERMOPHILIC PREC...   228  1.0e-19   2
/:pdb|1CIU|1CIU cyclodextrin glycosyltransferase(cgtase)       216  1.8e-18   2
/:swiss|P26827|AMY_THETU ALPHA-AMYLASE PRECURSOR (EC 3.2....   216  2.0e-18   2
/:pdb|1CGT|1CGT Cyclodextrin glycosyltransferase               199  4.9e-16   2
/:pdb|1CGU|1CGU Cyclodextrin glycosyltransferase mutant w...   199  4.9e-16   2
/:swiss|P27036|CDGT_BACOH CYCLOMALTODEXTRIN GLUCANOTRANSF...   208  5.4e-16   1
/:trembl|D90243|BOCGTAA_1 B.ohbensis CGT gene for cyclode...   208  5.4e-16   1
/:swiss|P30920|CDGT_BACCI CYCLOMALTODEXTRIN GLUCANOTRANSF...   199  5.7e-16   2
/:swiss|P14014|CDGT_BACLI CYCLOMALTODEXTRIN GLUCANOTRANSF...   199  5.7e-16   2
/:trembl|A18991|A18991_1 product: "gamma-cyclodextrin gly...   207  6.8e-16   1
/:swiss|P17692|AMYR_BACS8 RAW-STARCH-DIGESTING AMYLASE PR...   199  7.1e-16   2
/:pdb|1CDG|1CDG Cyclodextrin glycosyltransferase (cgtase)...   197  1.0e-15   2
/:pdb|1CGV|1CGV Cyclomaltodextrin glucanotransferase (cgt...   197  1.0e-15   2
/:pdb|1CGW|1CGW Cyclomaltodextrin glucanotransferase (cgt...   197  1.0e-15   2
/:pdb|1CGX|1CGX Cyclomaltodextrin glucanotransferase (cgt...   197  1.0e-15   2
/:pdb|1CGY|1CGY Cyclomaltodextrin glucanotransferase (cgt...   197  1.0e-15   2
/:pdb|1CXF|1CXF cyclodextrin glycosyltransferase(cgtase)M...   197  1.0e-15   2
/:swiss|P43379|CDGU_BACCI CYCLOMALTODEXTRIN GLUCANOTRANSF...   197  1.2e-15   2
/:swiss|P30270|AMY_STRGR ALPHA-AMYLASE PRECURSOR (EC 3.2....   202  1.6e-15   1
/:swiss|P09794|AMY_STRLM ALPHA-AMYLASE PRECURSOR (EC 3.2....   202  1.6e-15   1
/:trembl|D49448|CRGG2A_1 product: "glucoamylase G2";  Cor...   202  1.7e-15   1
/:trembl|Z85949|SLAMLB_1 gene: "amlB"; product: "alpha-am...   201  2.1e-15   1
/:swiss|P31835|CDG2_BACMA CYCLOMALTODEXTRIN GLUCANOTRANSF...   202  2.4e-15   1
/:pdb|1PAM|1PAM-A cyclodextrin glucanotransferasebiologic...   201  2.9e-15   1
/:swiss|P09121|CDGT_BACS3 CYCLOMALTODEXTRIN GLUCANOTRANSF...   201  3.1e-15   1
/:swiss|P05618|CDGT_BACS0 CYCLOMALTODEXTRIN GLUCANOTRANSF...   201  3.1e-15   1
/:swiss|P30921|CDGT_BACSP CYCLOMALTODEXTRIN GLUCANOTRANSF...   200  4.0e-15   1
/:swiss|P31747|CDGT_BACSS CYCLOMALTODEXTRIN GLUCANOTRANSF...   191  4.1e-15   2
/:pdb|1KUL|1KUL glucoamylasefragment: binding domain, res...   185  1.2e-14   1

... and many more amylases and related enzymes

Reciprocal BLAST-searches with the related sequence portions do not produce new 
hits but verify the family. 




Step 5 : The multiple alignment
*******************************

five groups of sequences related to PTG

group G1-ptg.lis
****************
swissnew:pig2_yeast
swissnew:gip2_yeast
swissnew:gac1_yeast
swissnew:pig1_yeast
RG1.gcg
PTG.gcg
hGl.gcg
rGl.gcg
cGl.gcg
swissprot:amyg_rhior


group G2-arxad.lis
******************
swissprot:amyg_arxad
JC4510                  (Lip.kon.)


group G3-slam.lis
*****************
amycloab_1
amycloab_2
amycloab_3
slamlc_21
slamlc_22
amyb_bacci.swissprot
amyb_bacp1
amyb_bacp2
bpamyb_1.trembl
bsg6ama_1.trembl
ucdsmamy_1.trembl

group G4-stss.lis
*****************
stss_1
stsssiii_i
(two highly homologous starch synthetase sequences)


group G5-bs22.lis
*****************
bs22045_1
1cgt                  -- 13
swissprot:AMYB_BACCE  -- 14
1kul                  -- 15

See the multiple alignment of sequence groups G1, G3, and G5



Step 6 : TBLASTN searches in the EST-database
*********************************************

PTG
/:embl|W28890|HSW28890    53b9 Human retina cDNA rando... +2   732  1.1e-71   1
/:embl|W19091|HS091325    zb14g10.r1 Soares fetal lung... +1   587  6.2e-61   2
/:embl|AA112835|HSAA12835 zn54e03.r1 Stratagene muscle... +2   600  2.5e-57   1
/:embl|R84901|HSR84901    yq27d11.r1 Soares fetal live... +2   458  2.8e-42   1
/:embl|T72137|HS13745     yc63h12.r1 Homo sapiens cDNA... +2   395  1.2e-35   1
/:embl|Z42059|HSC07C021   H. sapiens partial cDNA sequ... +3   341  1.3e-29   1
/:embl|R06045|HS04575     ye89f05.r1 Homo sapiens cDNA... +2   214  1.9e-16   1
/:embl|N88218|HSN2182     K2200F Fetal heart, Lambda Z... +2   201  6.2e-15   1
/:embl|H14696|HS696163    ym19g01.r1 Homo sapiens cDNA... +2   178  1.3e-12   1
/:embl|AA094222|HSAA94222 cl2316.seq.F Fetal heart, La... +2   180  2.2e-12   1
/:embl|AA089749|HSAA89749 chn1002.seq.F Fetal heart, L... +2   164  1.8e-10   1
/:embl|R10183|HS18380     yf35c07.r1 Homo sapiens cDNA... +3   149  6.7e-09   1
--> this is a new human paralogue of PTG
/:embl|R04030|CB0301      pk16h02.s1 Caenorhabditis br... +1    81  0.30      1

rGl
/:embl|W28890|HSW28890    53b9 Human retina cDNA rando... +2   453  4.0e-42   1
/:embl|W19091|HS091325    zb14g10.r1 Soares fetal lung... +1   337  7.2e-33   2
/:embl|AA112835|HSAA12835 zn54e03.r1 Stratagene muscle... +2   348  1.3e-30   1
/:embl|R84901|HSR84901    yq27d11.r1 Soares fetal live... +2   254  1.2e-20   1
/:embl|Z42059|HSC07C021   H. sapiens partial cDNA sequ... +3   240  6.5e-19   1
/:embl|T72137|HS13745     yc63h12.r1 Homo sapiens cDNA... +2   190  6.3e-14   1
/:embl|R10183|HS18380     yf35c07.r1 Homo sapiens cDNA... +3   141  5.6e-08   1
all in the same region

cGl
/:embl|R04030|CB0301      pk16h02.s1 Caenorhabditis br... +1   209  1.9e-15   1
/:embl|W28890|HSW28890    53b9 Human retina cDNA rando... +2   167  1.0e-09   1
/:embl|Z42059|HSC07C021   H. sapiens partial cDNA sequ... +3   125  2.9e-06   1
/:embl|AA112835|HSAA12835 zn54e03.r1 Stratagene muscle... +2   106  0.00021   1

hGl
/:embl|W28890|HSW28890    53b9 Human retina cDNA rando... +2   191  4.9e-14   1
/:embl|AA112835|HSAA12835 zn54e03.r1 Stratagene muscle... +2   130  2.4e-07   1
/:embl|W19091|HS091325    zb14g10.r1 Soares fetal lung... +1   128  6.7e-07   1
/:embl|Z42059|HSC07C021   H. sapiens partial cDNA sequ... +3   114  3.2e-05   1
/:embl|H21088|HS088173    yn65b01.s1 Homo sapiens cDNA... +1   110  4.8e-05   1
/:embl|H86973|HS973241    yt07f12.s1 Homo sapiens cDNA... +3   106  0.00017   1
/:embl|H20799|HS799168    yn64g01.s1 Homo sapiens cDNA... +3   109  0.00024   1


GIP2
/:embl|W28890|HSW28890    53b9 Human retina cDNA rando... +2   152  1.2e-09   1
/:embl|Z42059|HSC07C021   H. sapiens partial cDNA sequ... +3   124  1.3e-06   1
/:embl|AA112835|HSAA12835 zn54e03.r1 Stratagene muscle... +2   104  9.0e-05   1
/:embl|R10183|HS18380     yf35c07.r1 Homo sapiens cDNA... +3    94  0.0019    1
/:embl|W19091|HS091325    zb14g10.r1 Soares fetal lung... +1    92  0.0062    1

Author: Frank.Eisenhaber@imp.ac.at on internet.
Last modified: Feb 11 2000